...Helen S. Toogood; Nigel S. Scrutton Nitroreductases catalyse the NAD(P)H-dependent nitro reduction in nitrofuran antibiotics, which activates them into cytotoxic molecules leading to cell death. The design of new effective nitrofuran antibiotics relies on knowledge of the kinetic mechanism...

... of nitrofuran antibiotics. The crystal structure of free Escherichia coli NfsA and several homologues have been determined previously, but there is no structure of the enzyme with ligands. We present here crystal structures of oxidised E. coli NfsA in the presence of several ligands, including the antibiotic...

... of novel anti-infective agents. However, bacteria show a remarkable plasticity for isoprenoid biosynthesis that should be taken into account when targeting this metabolic pathway for the development of new antibiotics. For example, a few bacteria use the MVA pathway instead of the MEP pathway, whereas...

... into the energetics of RND-type transporters and their assembly into multiprotein complexes. antibiotic MexB multidrug resistance pathogen Pseudomonas aeruginosa resistance–nodulation–cell division transporter (RND transporter) 1 To whom correspondence should be addressed (email hv214...

... (email vogel@ucalgary.ca ). 31 3 2010 19 5 2010 24 5 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 acyl carrier protein antibiotic fatty acid biosynthesis lipid protein complex De novo FA (fatty acid) synthesis represents a crucial...

.... Because anal-3 shows high selectivity towards bacterial and fungal cells, it may provide an avenue for the development of new antibiotics. 1 To whom correspondence should be addressed (email ymkim@konkuk.ac.kr ). The atomic co-ordinates for 20 final structures reported will appear...

...Tracey L. Campbell; Denis M. Daigle; Eric D. Brown We present an analysis of the cellular phenotype and biochemical activity of a conserved bacterial GTPase of unknown function (YloQ and YjeQ in Bacillus subtilis and Escherichia coli respectively) using a collection of antibiotics of diverse...

...Arthur ABELIAN; Andrew P. WALSH; Georg LENTZEN; Fareed ABOUL-ELA; Michael J. GAIT The bacterial ribosome comprises 30 S and 50 S ribonucleoprotein subunits, contains a number of binding sites for known antibiotics and is an attractive target for selection of novel antibacterial agents. On the 30 S...

...Roger S. CHITTOCK; Simon WARD; Alan-Shaun WILKINSON; Patrick CASPERS; Barbara MENSCH; Malcolm G. P. PAGE; Christopher W. WHARTON A soluble form of Streptococcus pneumoniae PBP2x, a molecular target of penicillin and cephalosporin antibiotics, has been expressed and purified. IR difference spectra...