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Keywords: antibodies
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Biochem J (2024) 481 (24): 1977–1995.
Published: 23 December 2024
... antibodies that lengthen filopodia-like structures formed in a cell-free reconstitution system, and used them to uncover a key molecular switch governing length regulation. Using immunolabelling, enzyme-linked immunosorbent assays, immunoprecipitation and immunoblock experiments, we identified four...
Includes: Multimedia, Supplementary data
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Biochem J (2024) 481 (10): 643–651.
Published: 10 May 2024
... these reagents have not been rigorously validated, leading to potentially misleading results. In this report we tested several commercially-available antibodies for GCN5L1, and found that two-thirds of those available did not unambiguously detect the protein by western blot in cultured mouse cells or ex vivo...
Includes: Supplementary data
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Biochem J (2021) 478 (8): 1485–1509.
Published: 21 April 2021
...J. Sebastian Temme; Dorothy L. Butler; Jeffrey C. Gildersleeve Carbohydrate-binding antibodies play diverse and critical roles in human health. Endogenous carbohydrate-binding antibodies that recognize bacterial, fungal, and other microbial carbohydrates prevent systemic infections and help...
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Biochem J (2019) 476 (1): 39–50.
Published: 07 January 2019
... and antibodies has clear clinical benefit. Here, we report the isolation and functional characterization of novel camelid single-domain antibodies ( sdAbs or V H Hs ) directed against human EGFR. The source of these V H Hs was a llama immunized with cDNA encoding human EGFR ectodomain alone (no protein or cell...
Includes: Supplementary data
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Biochem J (2017) 474 (4): 517–519.
Published: 03 February 2017
... the host immune system. Many anti-fHbp monoclonal antibodies (mAbs) produced against fHbp fail to trigger complement-mediated bacteriolysis when used alone in vitro , but are highly synergistic and bactericidal when used in combination. This opened the door to defining the structural basis by which mAbs...
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Biochem J (1999) 344 (2): 293–296.
Published: 24 November 1999
... functional assays for the cytotoxic domain of Aβ and suggest a mechanism for some of the observed actions of Aβ plus catalase in vitro . 1 e-mail [email protected] 1 9 1999 24 9 1999 1 10 1999 The Biochemical Society, London © 1999 1999 Alzheimers antibodies...