... of Medicine, University of Helsinki, 00014 University of Helsinki, Finland. 4 8 2022 9 11 2022 11 11 2022 © 2022 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2022 archaea carotenogenesis degron LonB protease phytoene synthase...

... of the protein to constrain DNA supercoils. Correspondence: Yong Gong ( [email protected] ) or Li Huang ( [email protected] ) Archaea chromatin protein Cren7 DNA bending Sulfolobus All living organisms face the challenge of packaging long genomic DNA into a small cell or nucleus while...

...Antonella Caforio; Samta Jain; Peter Fodran; Melvin Siliakus; Adriaan J. Minnaard; John van der Oost; Arnold J.M. Driessen In archaea, the membrane phospholipids consist of isoprenoid hydrocarbon chains that are ether-linked to a sn -glycerol1-phosphate backbone. This unique structure is believed...

... core binds both E1 and E3 component enzymes to form for the first time a catalytically active mini-complex. archaea macromolecular assembly multienzyme complex 2-oxoacid dehydrogenase thermophile X-ray crystallography From the structures of the octahedral and icosahedral cores...

... Society 2012 Archaea DNA ligase DNA polymerase nuclease Okazaki fragment Accurate elimination of RNA initiators at the replication fork is a crucial process to ensure genome integrity. In eukaryotic cells, RNA initiators, 7–12 nt in length, are generated by the primase activity...

...Li Phing Liew; Stephen D. Bell The MCM (minichromosome maintenance) proteins of archaea are widely believed to be the replicative DNA helicase of these organisms. Most archaea possess a single MCM orthologue that forms homo-multimeric assemblies with a single hexamer believed to be the active form...

...Clare Jelinska; Biljana Petrovic-Stojanovska; W. John Ingledew; Malcolm F. White Archaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro , and we show...

...Hala Chamieh; Dorian Guetta; Bruno Franzetti The PAN (proteasome-activating nucleotidase) proteins from archaea represent homologues of the eukaryotic 26S proteasome regulatory ATPases. In vitro the PAN complex has been previously shown to have a stimulatory effect on the peptidase activities...

...Alan I. Majerník; James P. J. Chong To date, methanogens are the only group within the archaea where firing DNA replication origins have not been demonstrated in vivo . In the present study we show that a previously identified cluster of ORB (origin recognition box) sequences do indeed function...

..., mixtures were incubated for 5 min at 95 °C which contained the labelled and the complementary unlabelled oligonucleotides at a 1:2 molar ratio. The samples were then slowly cooled at room temperature (25 °C). © The Authors Journal compilation © 2007 Biochemical Society 2007 Archaea DNA...

... may autoregulate their own translation in a manner analogous to that of the well characterized thymidylate synthase A proteins, although there is no significant amino acid sequence similarity between them. archaea in vitro translation Pyrococcus furiosus RNA-binding protein stem–loop...

... electrophoresis, the gel was soaked in 20% trichloroacetic acid and analysed using a PhosphorImager. The reaction products were quantified, and any free oligonucleotide in the absence of enzyme was subtracted. Archaea DNA helicase DNA replication helicaseloader Sulfolobus solfataricus thermophilic...

...). The Biochemical Society, London 2004 archaea catalytic mechanism Halobacterium Okazaki fragment RNA–DNA junction RNase H Plasmid pHASH-Halo1 was constructed for complementation assays by ligating the DNA fragment containing the Halobacterium Vng0255c gene ( Halo-rnhA ) to the Eco RV...

...Corné H. VERHEES; Servé W. M. KENGEN; Judith E. TUININGA; Gerrit J. SCHUT; Michael W. W. ADAMS; Willem M. de VOS; John van der OOST An early divergence in evolution has resulted in two prokaryotic domains, the Bacteria and the Archaea. Whereas the central metabolic routes of bacteria and eukaryotes...

... for the FAU-1 protein from Pyrococcus furiosus has been deposited in the DDBJ, EMBL, GenBank® and GSDB Nucleotide Sequence Databases under the accession number AB055587. 19 12 2002 3 3 2003 4 3 2003 4 3 2003 The Biochemical Society, London ©2003 2003 archaea RNA-binding...

... an opportunity to exploit Nature's phylogenetic diversity as a vehicle for critically examining this hypothesis. In tracing the origins and evolution of protein phosphorylation/dephosphorylation, the members of the Archaea, the so-called ‘third domain of life’, will play a critical role. Whereas several studies...

... ). 1 7 2002 1 8 2002 6 8 2002 6 8 2002 The Biochemical Society, London ©2002 2002 archaea hyperthermophile protease protein degradation Sulfolobus Abbreviations used: Ac, acetyl; AMC, 7-amido-4-methylcoumarin; CBz, benzyloxycarbonyl; E-64, trans...

... heterologous protein to be expressed in the Haloarchaea. 1 To whom correspondence should be addressed (e-mail Stewart.Nuttall@;hsn.csiro.au). 18 11 1999 4 1 2000 14 1 2000 The Biochemical Society, London © 2000 2000 antibiotic resistance Archaea Haloarchaea protein...