... assessed by gel filtration chromatography using a Superdex 200 HR (10/30) column, equilibrated with 0.1 M Tris/HCl buffer (pH 7.5). Protein concentrations were determined by the protein-dye binding method of Bradford using BSA as the standard [ 20 ]. The argininosuccinate lyase activity of δ...

...Liliana M. SAMPALEANU; Penelope W. CODDING; Yuri D. LOBSANOV; May TSAI; G. David SMITH; Cathy HORVATIN; P. Lynne HOWELL δ Crystallin, a taxon-specific crystallin present in avian eye lenses, is homologous to the urea cycle enzyme ASL (argininosuccinate lyase). Although there are two δ crystallin...