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Keywords: assay
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In Collection
Awards
David J. Fallon, Alex Phillipou, Christopher J. Schofield, David House, Nicholas C. O. Tomkinson, Jacob T. Bush
Journal:
Biochemical Journal
Biochem J (2023) 480 (15): 1183–1197.
Published: 31 August 2023
...David J. Fallon; Alex Phillipou; Christopher J. Schofield; David House; Nicholas C. O. Tomkinson; Jacob T. Bush The development and optimisation of a photoaffinity labelling (PAL) displacement assay is presented, where a highly efficient PAL probe was used to report on the relative binding...
Includes: Supplementary data
Articles
DNA-linked inhibitor antibody assay (DIANA) as a new method for screening influenza neuraminidase inhibitors
Open AccessMilan Kožíšek, Václav Navrátil, Kateřina Rojíková, Jana Pokorná, Carlos Berenguer Albiñana, Petr Pachl, Jitka Zemanová, Aleš Machara, Pavel Šácha, Jason Hudlický, Ivana Císařová, Pavlína Řezáčová, Jan Konvalinka
Journal:
Biochemical Journal
Biochem J (2018) 475 (23): 3847–3860.
Published: 10 December 2018
... influenza strains. A facile, sensitive, and inexpensive screening method would help achieve this goal. Recently, we described a multiwell plate-based DNA-linked inhibitor antibody assay (DIANA). This highly sensitive method can quantify femtomolar concentrations of enzymes. DIANA also has been applied...
Includes: Supplementary data
Articles
Kinetic analysis using low-molecular mass xyloglucan oligosaccharides defines the catalytic mechanism of a Populus xyloglucan endotransglycosylase
Available to PurchaseMarc Saura-Valls, Régis Fauré, Sergi Ragàs, Kathleen Piens, Harry Brumer, Tuula T. Teeri, Sylvain Cottaz, Hugues Driguez, Antoni Planas
Journal:
Biochemical Journal
Biochem J (2006) 395 (1): 99–106.
Published: 15 March 2006
..., and are thought to be important enzymes in the formation and remodelling of the cellulose-XG three-dimensional network in the primary plant cell wall. Current methods to assay XET activity use the XG polysaccharide as the donor substrate, and present limitations for kinetic and mechanistic studies of XET action...
Includes: Supplementary data