...Dipanwita Bhattacharya; Kanchan Sinha; Dulal Panda The role of FtsZ-associated proteins in the regulation of the assembly dynamics of Mycobacterium smegmatis FtsZ is not clear. In this work, we examined the effect of M. smegmatis SepF on the assembly and stability of M. smegmatis FtsZ polymers. We...

...Sacha A. Jensen; Penny A. Handford The 10–12 nm diameter microfibrils of the extracellular matrix (ECM) impart both structural and regulatory properties to load-bearing connective tissues. The main protein component is the calcium-dependent glycoprotein fibrillin, which assembles into microfibrils...

... (really interesting new gene) E3 ubiquitin ligases] draw particular attention, being the largest family of E3s. The CRLs assemble into functional multisubunit complexes using a repertoire of substrate receptors, adaptors, Cullin scaffolds and RING-box proteins. Drug discovery targeting CRLs is growing...

...Laetitia Sabatier; Jelena Djokic; Christine Fagotto-Kaufmann; Marian Chen; Douglas S. Annis; Deane F. Mosher; Dieter P. Reinhardt Fibrillins constitute the backbone of extracellular multifunctional assemblies present in elastic and non-elastic matrices, termed microfibrils. Assembly of fibrillins...

...Xiaodi Yu; Ganeshram R. Visweswaran; Zoe Duck; Srisailam Marupakula; Sheila MacIntyre; Stefan D. Knight; Anton V. Zavialov The chaperone/usher pathway controls assembly of fibres of adhesive organelles of Gram-negative bacteria. The final steps of fibre assembly and fibre translocation to the cell...

... and for assembly of the retromer complex. Mutation of residues within the conserved region results in Vps35p/VPS35 mutants, which cannot bind to Vps26p/VPS26 and are not efficiently targeted to the endosomal membrane. These data implicate Vps26p/VPS26 in regulating Vps35p/VPS35 membrane association and therefore...

... the intercellular propagation of electrical signals to the selective intercellular passage of small regulatory molecules. Importantly, the extent of gap-junctional intercellular communication is under the direct control of regulatory events associated with channel assembly and turnover, as the vast majority...

... in vitro with a number of extracellular matrix proteins, e.g. Fn (fibronectin). In view of its strong binding to Fn, we examined in the present study whether the released soluble ectodomain can bind to the fibrillar Fn matrix under cell-culture conditions and, if so, influence its assembly. In this study...

...Susannah Horan; Ingrid Bourges; Jan-Willem Taanman; Brigitte Meunier Cytochrome oxidase catalyses the reduction of oxygen to water. The mitochondrial enzyme contains up to 13 subunits, 11 in yeast, of which three, Cox1p, Cox2p and Cox3p, are mitochondrially encoded. The assembly pathway...

...Anton V. Zavialov; Vladimir M. Tischenko; Laura J. Fooks; Bjørn O. Brandsdal; Johan Åqvist; Vladimir P. Zav'yalov; Sheila Macintyre; Stefan D. Knight Periplasmic chaperone/usher machineries are used for assembly of filamentous adhesion organelles of Gram-negative pathogens in a process that has...

... these mutations affect the self- and/or hetero-assembly of the corresponding proteins. To our knowledge, despite significant progress in understanding the role of various amino acids in TM–TM interactions in vivo , the direct effect of positively charged residues on these interactions has not been studied...

... should be addressed (e-mail [email protected] ). 18 10 2001 6 12 2001 19 1 2002 The Biochemical Society, London ©2002 2002 assembly cardioencephalomyopathy COX deficiency mitochondria respiratory chain Abbreviations used: COX, cytochrome c oxidase; CS, citrate...

... words: adult, assembly, embryonic, NMR. Haem disorder is absent in myoglobins reconstituted with completely symmetric synthetic haems [10]. Along with nuclear- Overhauser-effect measurements [11], these experiments have demonstrated unambiguously that haem disorder arises from distinct haem orientations...