... to current models of brain energy metabolism. However, structural and biophysical considerations suggest that the pump should also have access to mitochondrial ATP, which is much more abundant. In the present study, we have investigated the source of ATP that fuels the Na + pump in astrocytes, taking...

... missing the first predicted transmembrane domain. astrocyte bestrophin chloride channel glioblastoma multiforme splicing VMD2 The bestrophin family is composed of four human genes that form transmembrane proteins [ 1 – 3 ]. When heteroexpressed in HEK (human embryonic kidney)-293 cells...

... we set out to explore the specificity and the mode of action of the reported inhibitory effect of the TCA (tricyclic antidepressant) amitriptyline on DNMT (DNA methyltransferase) activity in primary astrocytes from the rat cortex. We found that the impact on DNMT was shared by another TCA, imipramine...

... astrocyte cultures. Using ferricyanide as the analytical oxidant we demonstrate that tPMET is enhanced by dehydroascorbate uptake via facilitative glucose transporters, and subsequent accumulation of intracellular ascorbate. Additionally, we demonstrate that this stimulation is not due to ascorbate...

... cells and neurons. The aim of the present study was to assess the biological significance of the impact of NO • on the cell's thiol status with emphasis on S-glutathionylation of targeted proteins. Exposure of primary cortical neurons or astrocytes to increasing flow rates of NO • (0.061–0.25 μM/s...

... neuropeptides whose respective central actions are currently unknown. In the present study, we have compared the mechanism of action of URP and UII on cultured astrocytes. Competition experiments performed with [ 125 I]UII showed the presence of very-high- and high-affinity binding sites for UII, and a single...

...-mail [email protected] ). 21 1 2000 5 5 2000 25 5 2000 The Biochemical Society, London © 2000 2000 antiporter arginine transport astrocyte brain metabolism glutamine transport Biochem. J. (2000) 349, 787 795 (Printed in Great Britain) 787 The heterodimeric amino acid...