... compounds exhibiting high binding affinities. Among these compounds, Saquinavir (SQV) emerged as a potent PAD4 inhibitor, demonstrating competitive inhibition with a low IC 50 value of 1.21 ± 0.04 µM. In vitro assays, including enzyme kinetics and biophysical analyses, highlighted significant changes...

... that a SPAG1 sub-fragment, containing a putative P-loop motif, cannot efficiently bind and hydrolyze GTP in vitro . Our data challenge the interpretation of SPAG1 possessing GTPase activity. We propose instead that SPAG1 regulates nucleotide hydrolysis activity of the HSP and RUVBL1/2 partners. biophysics...

... role in membrane fusion. To investigate this dual function, we used two independent cell cultures coupled with biophysical techniques. The data reveal that the PBD mediates T-cell suppression by stabilizing a TCR-binding conformation in the membrane. Moreover, we show that the clinically used HIV...

... by the properties of the AMPs using a series of peptides composed of six lysines and nine leucines, which differ in their sequences and hence their biophysical properties. Treatment with bactericidal peptides at non-inhibitory concentrations resulted in reduced biofilm growth, for some starting from 25 nM which...

... aid in deciphering the function of DHSM in biological membranes. biophysics HIV HIV entry inhibitor membrane fusion membrane protein protein folding HIV-1 entry into the cells is blocked by alteration in the levels of Chol (cholesterol) and sphingolipids that are essential...

... the diagonal of the 2D-IR spectrum. If c 2D is zero, the line will be uncorrelated and circular in shape. Finally, a c 2D value of −1 indicates an anticorrelated lineshape, which will be elongated along the antidiagonal of the spectrum. biophysics haem protein two-dimensional infrared (2D-IR...

... studied their behaviour in solution by complementary biochemical and biophysical techniques, identified the regions which promote self-association and determined their overall three-dimensional shape. The present study paves the way to further studies and rationalizes the existing knowledge on the self...