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1-4 of 4
Keywords: biotin-binding protein
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Articles
Satu H. Helppolainen, Kirsi P. Nurminen, Juha A. E. Määttä, Katrin K. Halling, J. Peter Slotte, Tuulia Huhtala, Timo Liimatainen, Seppo Ylä-Herttuala, Kari J. Airenne, Ale Närvänen, Janne Jänis, Pirjo Vainiotalo, Jarkko Valjakka, Markku S. Kulomaa, Henri R. Nordlund
Journal:
Biochemical Journal
Biochem J (2007) 405 (3): 397–405.
Published: 13 July 2007
... dissociation and ITC (isothermal titration calorimetry) experiments showed dimeric rhizavidin to be a high-affinity biotin-binding protein. Its thermal stability was lower than that of avidin; although similar to streptavidin, it was insensitive to proteinase K. The immunological cross-reactivity of rhizavidin...
Articles
Vesa P. HYTÖNEN, Olli H. LAITINEN, Alessandro GRAPPUTO, Anu KETTUNEN, Janne SAVOLAINEN, Nisse KALKKINEN, Ari T. MARTTILA, Henri R. NORDLUND, Thomas K. M. NYHOLM, Giovanni PAGANELLI, Markku S. KULOMAA
Journal:
Biochemical Journal
Biochem J (2003) 372 (1): 219–225.
Published: 15 May 2003
...-mail [email protected] ). 2 10 2002 17 1 2003 31 1 2003 31 1 2003 The Biochemical Society, London ©2003 2003 avidin—biotin technology biotin-binding protein cancer treatment immunoreactivity targeted drug delivery Abbreviations used: AP, alkaline phosphatase; DSC...
Articles
Ari T. MARTTILA, Vesa P. HYTÖNEN, Olli H. LAITINEN, Edward A. BAYER, Meir WILCHEK, Markku S. KULOMAA
Journal:
Biochemical Journal
Biochem J (2003) 369 (2): 249–254.
Published: 15 January 2003
... of Kuopio, P.O Box 1627, FIN-70211 Kuopio, Finland. 2 To whom correspondence should be addressed (e-mail [email protected] ). 10 6 2002 9 8 2002 2 10 2002 2 10 2002 The Biochemical Society, London ©2003 2003 Avidin–biotin technology biotin-binding protein reversibility...
Articles
Olli H. LAITINEN, Vesa P. HYTÖNEN, Mervi K. AHLROTH, Olli T. PENTIKÄINEN, Ciara GALLAGHER, Henri R. NORDLUND, Vladimir OVOD, Ari T. MARTTILA, Eevaleena PORKKA, Sanna HEINO, Mark S. JOHNSON, Kari J. AIRENNE, Markku S. KULOMAA
Journal:
Biochemical Journal
Biochem J (2002) 363 (3): 609–617.
Published: 24 April 2002
... applications. Key words: avidin biotin technology, biotin-binding protein, molecular evolution, molecular modelling, structure function relationship. bridges, whereas avidin has a carbohydrate side chain and one intramolecular disulphide bond. Furthermore, the pI of strep- tavidin is acidic (pIE 6) in contrast...
