... affinities of compounds to specific binding sites in multiple recombinant protein domains in tandem. The N- and C-terminal bromodomains of BRD4 were used as example target proteins. A test set of 264 compounds annotated with activity against the bromodomain and extra-terminal domain (BET) family in ChEMBL...

...Xiao Yang; Xuelei Wu; Jiahai Zhang; Xuecheng Zhang; Chao Xu; Shanhui Liao; Xiaoming Tu Histone modification plays an important role in various biological processes, including gene expression regulation. Bromodomain, as one of histone readers, recognizes specifically the ε- N -lysine acetylation...