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Articles

Revisiting the roles of cAMP signalling in the progression of prostate cancer

Emma C. Parsons, Ralf Hoffmann, George S. Baillie

Biochem J (2023) 480 (20): 1599–1614.

https://doi.org/10.1042/BCJ20230297

Published: 13 October 2023

... androgen signalling systems and a variety of other transduction pathways which drive differentiation of these cells towards castration-resistance. One such signalling pathway is the ubiquitous cAMP signalling axis which functions to activate spatially restricted pools of cAMP effectors such as protein...

View article titled, Revisiting the roles of cAMP signalling in the progression of prostate cancer

Articles

Allosteric pluripotency: challenges and opportunities

Madoka Akimoto, Karla Martinez Pomier, Bryan VanSchouwen, Jung Ah Byun, Mariia Khamina, Giuseppe Melacini

Biochem J (2022) 479 (7): 825–838.

https://doi.org/10.1042/BCJ20210528

Published: 11 April 2022

... ]. Interestingly, similar agonism-antagonism switches have also been reported for other systems, suggesting that allosteric pluripotency is a general phenomenon not limited exclusively to estrogen receptors. For example, the response of protein kinase A (PKA) to the phosphorothioate analog Rp-cAMPS (or Rp...

View article titled, Allosteric pluripotency: challenges and opportunities

Articles

The cAMP-phosphodiesterase 4 (PDE4) controls β-adrenoceptor- and CFTR-dependent saliva secretion in mice

Abigail Boyd, Ileana V. Aragon, Lina Abou Saleh, Dylan Southers, Wito Richter

Biochem J (2021) 478 (10): 1891–1906.

https://doi.org/10.1042/BCJ20210212

Published: 24 May 2021

..., that is ablated by the β-blocker Propranolol and is absent from homozygous ΔF508-CFTR mice lacking functional CFTR. These data suggest that PDE4 acts within salivary glands to gate saliva secretion that is contingent upon the cAMP/PKA-dependent activation of CFTR. Indeed, PDE4 contributes the majority of total...

View article titled, The cAMP-phosphodiesterase 4 (PDE4) controls β-adrenoceptor- and CFTR-dependent saliva secretion in mice

Includes: Supplementary data

Articles

Protein kinase A negatively regulates VEGF-induced AMPK activation by phosphorylating CaMKK2 at serine 495

Katrin Spengler, Darya Zibrova, Angela Woods, Christopher G. Langendorf, John W. Scott, David Carling, Regine Heller

Biochem J (2020) 477 (17): 3453–3469.

https://doi.org/10.1042/BCJ20200555

Published: 17 September 2020

... (CaMKK2) represents a pro-angiogenic pathway, whose regulation and function is incompletely understood. This study investigates whether the VEGF/AMPK pathway is regulated by cAMP-mediated signalling. We show that cAMP elevation in endothelial cells by forskolin, an activator of the adenylate cyclase...

View article titled, Protein kinase A negatively regulates VEGF-induced AMPK activation by phosphorylating CaMKK2 at serine 495

Includes: Supplementary data

Articles

Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation

João D. Santos, Francisco R. Pinto, João F. Ferreira, Margarida D. Amaral, Manuela Zaccolo, Carlos M. Farinha

Biochem J (2020) 477 (13): 2561–2580.

https://doi.org/10.1042/BCJ20200287

Published: 10 July 2020

... Regulator (CFTR) protein, a cAMP-regulated chloride channel expressed at the apical surface of epithelial cells. Cyclic AMP regulates both CFTR channel gating through a protein kinase A (PKA)-dependent process and plasma membane (PM) stability through activation of the exchange protein directly activated...

View article titled, Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation

Includes: Supplementary data

Articles

Ezrin-anchored PKA phosphorylates serine 369 and 373 on connexin 43 to enhance gap junction assembly, communication, and cell fusion

Aleksandra R. Dukic, Pascale Gerbaud, Jean Guibourdenche, Bernd Thiede, Kjetil Taskén, Guillaume Pidoux

Biochem J (2018) 475 (2): 455–476.

https://doi.org/10.1042/BCJ20170529

Published: 31 January 2018

...) that provides cAMP-mediated control of gap junction communication. Here, we examined the associated phosphorylation events. Inhibition of PKA activity resulted in decreased Cx43 phosphorylation, which was associated with reduced trophoblast fusion and differentiation. In vitro studies using peptide arrays...

View article titled, Ezrin-anchored PKA phosphorylates serine 369 and 373 on connexin 43 to enhance gap junction assembly, communication, and cell fusion

Includes: Supplementary data

Articles

Synthesis and degradation of cAMP in Giardia lamblia : possible role and characterization of a nucleotidyl cyclase with a single cyclase homology domain

Vanina Saraullo, Nicolas Di Siervi, Belen Jerez, Carlos Davio, Adolfo Zurita

Biochem J (2017) 474 (23): 4001–4017.

https://doi.org/10.1042/BCJ20170590

Published: 21 November 2017

...Vanina Saraullo; Nicolas Di Siervi; Belen Jerez; Carlos Davio; Adolfo Zurita Despite its importance in the regulation of growth and differentiation processes of a variety of organisms, the mechanism of synthesis and degradation of cAMP (cyclic AMP) has not yet been described in Giardia lamblia...

View article titled, Synthesis and degradation of cAMP in Giardia lamblia : possible role and characterization of a nucleotidyl cyclase with a single cyclase homology domain

Includes: Supplementary data

Articles

Mutations of PKA cyclic nucleotide-binding domains reveal novel aspects of cyclic nucleotide selectivity

Robin Lorenz, Eui-Whan Moon, Jeong Joo Kim, Sven H. Schmidt, Banumathi Sankaran, Ioannis V. Pavlidis, Choel Kim, Friedrich W. Herberg

Biochem J (2017) 474 (14): 2389–2403.

https://doi.org/10.1042/BCJ20160969

Published: 06 July 2017

...′-cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and the 3′,5′-cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG), are preferentially activated by cAMP and cGMP, respectively. However, the molecular basis of this cyclic nucleotide selectivity is still not fully...

View article titled, Mutations of PKA cyclic nucleotide-binding domains reveal novel aspects of cyclic nucleotide selectivity

Includes: Supplementary data

Articles

Identification of a multifunctional docking site on the catalytic unit of phosphodiesterase-4 (PDE4) that is utilised by multiple interaction partners

Kirsty F. Houslay, Frank Christian, Ruth MacLeod, David R. Adams, Miles D. Houslay, George S. Baillie

Biochem J (2017) 474 (4): 597–609.

https://doi.org/10.1042/BCJ20160849

Published: 03 February 2017

...Kirsty F. Houslay; Frank Christian; Ruth MacLeod; David R. Adams; Miles D. Houslay; George S. Baillie Cyclic AMP (cAMP)-specific phosphodiesterase-4 (PDE4) enzymes underpin compartmentalised cAMP signalling by localising to distinct signalling complexes. PDE4 long isoforms can be phosphorylated...

View article titled, Identification of a multifunctional docking site on the catalytic unit of phosphodiesterase-4 (PDE4) that is utilised by multiple interaction partners

Articles

Effects of heterologous expression of human cyclic nucleotide phosphodiesterase 3A (hPDE3A) on redox regulation in yeast

Dong Keun Rhee, Jung Chae Lim, Steven C. Hockman, Faiyaz Ahmad, Dong Ho Woo, Youn Wook Chung, Shiwei Liu, Allison L. Hockman, Vincent C. Manganiello

Biochem J (2016) 473 (22): 4205–4225.

https://doi.org/10.1042/BCJ20160572

Published: 10 November 2016

... of YAP1 and YAP1 -dependent genes was impaired, and the thioredoxin system malfunctioned. H 2 O 2 increased cyclic adenosine monophosphate (cAMP)-hydrolyzing activity of WT hPDE3A, but not K13R hPDE3A, through PKA-dependent phosphorylation of hPDE3A, which was correlated with its ubiquitinylation...

View article titled, Effects of heterologous expression of human cyclic nucleotide phosphodiesterase 3A (hPDE3A) on redox regulation in yeast

Includes: Supplementary data

Articles

HCN2 ion channels: basic science opens up possibilities for therapeutic intervention in neuropathic pain

Christoforos Tsantoulas, Elizabeth R. Mooney, Peter A. McNaughton

Biochem J (2016) 473 (18): 2717–2736.

https://doi.org/10.1042/BCJ20160287

Published: 12 September 2016

... or nociceptors. Hypersensitivity: An exaggerated pain response of the nociceptive nervous system. cAMP HCN2 ivabradine inflammation Hyperalgesia: Increased pain response to noxious (painful) stimuli (overlaps with hypersensitivity). Allodynia: Augmented pain response to innocuous...

View article titled, HCN2 ion channels: basic science opens up possibilities for therapeutic intervention in neuropathic pain

Articles

Exchange protein directly activated by cAMP modulates regulatory T-cell-mediated immunosuppression

Muayad Almahariq, Fang C. Mei, Hui Wang, Anthony T. Cao, Suxia Yao, Lynn Soong, Jiaren Sun, Yingzi Cong, Ju Chen, Xiaodong Cheng

Biochem J (2015) 465 (2): 295–303.

https://doi.org/10.1042/BJ20140952

Published: 06 January 2015

...Muayad Almahariq; Fang C. Mei; Hui Wang; Anthony T. Cao; Suxia Yao; Lynn Soong; Jiaren Sun; Yingzi Cong; Ju Chen; Xiaodong Cheng The cAMP signalling pathway plays an essential role in immune functions. In the present study we examined the role of the cAMP/EPAC1 (exchange protein directly activated...

View article titled, Exchange protein directly activated by cAMP modulates regulatory T-cell-mediated immunosuppression

Includes: Supplementary data

Articles

cAMP ameliorates inflammation by modulation of macrophage receptor for advanced glycation end-products

So Motoyoshi, Yasuhiko Yamamoto, Seiichi Munesue, Hirobumi Igawa, Ai Harashima, Hidehito Saito, Dong Han, Takuo Watanabe, Hiroshi Sato, Hiroshi Yamamoto

Biochem J (2014) 463 (1): 75–82.

https://doi.org/10.1042/BJ20140084

Published: 08 September 2014

... study, we show that cAMP-induced modulation of the RAGE isoform in macrophages can control the inflammatory state in both in vitro and in vivo experimental conditions. The RAGE ligand S100B stimulated MCP-1 (monocyte chemoattractant protein-1) secretion from peritoneal macrophages, but cAMP elevation...

View article titled, cAMP ameliorates inflammation by modulation of macrophage receptor for advanced glycation end-products

Includes: Supplementary data

Articles

C/EBPβ (CCAAT/enhancer-binding protein β) mediates progesterone production through transcriptional regulation in co-operation with SF-1 (steroidogenic factor-1)

Tetsuya Mizutani, Yunfeng Ju, Yoshitaka Imamichi, Tsukasa Osaki, Takashi Yazawa, Shinya Kawabe, Shin Ishikane, Takehiro Matsumura, Masafumi Kanno, Yasue Kamiki, Kohei Kimura, Naoto Minamino, Kaoru Miyamoto

Biochem J (2014) 460 (3): 459–471.

https://doi.org/10.1042/BJ20131522

Published: 29 May 2014

... together with SF-1 are poorly understood. C/EBPβ knockdown attenuated cAMP-induced progesterone production in granulosa tumour-derived KGN cells by altering STAR (steroidogenic acute regulatory protein), CYP11A1 (cytochrome P450, family 11, subfamily A, polypeptide 1) and HSD3B2 (hydroxy-δ-5-steroid...

View article titled, C/EBPβ (CCAAT/enhancer-binding protein β) mediates progesterone production through transcriptional regulation in co-operation with SF-1 (steroidogenic factor-1)

Includes: Supplementary data

Articles

The upstream conserved regions (UCRs) mediate homo- and hetero-oligomerization of type 4 cyclic nucleotide phosphodiesterases (PDE4s)

Moses Xie, Brigitte Blackman, Colleen Scheitrum, Delphine Mika, Elise Blanchard, Tao Lei, Marco Conti, Wito Richter

Biochem J (2014) 459 (3): 539–550.

https://doi.org/10.1042/BJ20131681

Published: 11 April 2014

... and short variants by the presence or absence of the N-terminal UCR domains. The UCR domains mediate homo- as well as hetero-oligomerization of long PDE4s, whereas short variants, which lack portions of the UCRs, are monomers. cAMP cyclic nucleotide phosphodiesterase 4 (PDE4) dimer oligomerization...

View article titled, The upstream conserved regions (UCRs) mediate homo- and hetero-oligomerization of type 4 cyclic nucleotide phosphodiesterases (PDE4s)

Includes: Supplementary data

Articles

A LOV-domain-mediated blue-light-activated adenylate (adenylyl) cyclase from the cyanobacterium Microcoleus chthonoplastes PCC 7420

Sarah Raffelberg, Linzhu Wang, Shiqiang Gao, Aba Losi, Wolfgang Gärtner, Georg Nagel

Biochem J (2013) 455 (3): 359–365.

https://doi.org/10.1042/BJ20130637

Published: 10 October 2013

... of the purified protein at saturating light and pH 8 is estimated to 1 cAMP/mPAC per s at 25°C (2 cAMP/mPAC per s at 35°C). The lifetime of light-activated cAMP production after a BL flash was ~14 s at 20°C. The temperature optimum was determined to 35°C and the pH optimum to 8.0. The value for half-maximal...

View article titled, A LOV-domain-mediated blue-light-activated adenylate (adenylyl) cyclase from the cyanobacterium Microcoleus chthonoplastes PCC 7420

Articles

Phosphorylation of the guanine-nucleotide-exchange factor CalDAG-GEFI by protein kinase A regulates Ca 2+ -dependent activation of platelet Rap1b GTPase

Gianni Francesco Guidetti, Daria Manganaro, Alessandra Consonni, Ilaria Canobbio, Cesare Balduini, Mauro Torti

Biochem J (2013) 453 (1): 115–123.

https://doi.org/10.1042/BJ20130131

Published: 13 June 2013

...Gianni Francesco Guidetti; Daria Manganaro; Alessandra Consonni; Ilaria Canobbio; Cesare Balduini; Mauro Torti In blood platelets the small GTPase Rap1b is activated by cytosolic Ca 2+ and promotes integrin α IIb β 3 inside-out activation and platelet aggregation. cAMP is the major inhibitor...

View article titled, Phosphorylation of the guanine-nucleotide-exchange factor CalDAG-GEFI by protein kinase A regulates Ca 2+ -dependent activation of platelet Rap1b GTPase

Articles

Differential regulation of glucose transport activity in yeast by specific cAMP signatures

Clara Bermejo, Farzad Haerizadeh, Mayuri S. C. Sadoine, Diane Chermak, Wolf B. Frommer

Biochem J (2013) 452 (3): 489–497.

https://doi.org/10.1042/BJ20121736

Published: 31 May 2013

... to glucose by activating the Hxt5p (hexose transporter 5) glucose transporter, which provides an advantage during early phases after glucose resupply. cAMP and glucose FRET (fluorescence resonance energy transfer) sensors were used to identify three signalling pathways that co-operate in the anticipatory...

View article titled, Differential regulation of glucose transport activity in yeast by specific cAMP signatures

Includes: Supplementary data

Articles

Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells

Tetsuya Kitaguchi, Manami Oya, Yoshiko Wada, Takashi Tsuboi, Atsushi Miyawaki

Biochem J (2013) 450 (2): 365–373.

https://doi.org/10.1042/BJ20121022

Published: 15 February 2013

...Tetsuya Kitaguchi; Manami Oya; Yoshiko Wada; Takashi Tsuboi; Atsushi Miyawaki Intracellular cAMP and Ca 2+ are important second messengers that regulate insulin secretion in pancreatic β-cells; however, the molecular mechanism underlying their mutual interaction for exocytosis is not fully...

View article titled, Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells

Includes: Supplementary data

Articles

Identification of a pathway by which glucose regulates β-catenin signalling via the cAMP/protein kinase A pathway in β-cell models

Emmanuelle Cognard, Coralie G. Dargaville, Deborah L. Hay, Peter R. Shepherd

Biochem J (2013) 449 (3): 803–811.

https://doi.org/10.1042/BJ20121454

Published: 09 January 2013

... its transcriptional activity. In a search for possible signalling intermediates we found forskolin and cell-permeable cAMP analogues recapitulated the glucose effects, suggesting a role for cAMP and PKA (cAMP-dependent protein kinase/protein kinase A) downstream of glucose. Furthermore, glucose caused...

View article titled, Identification of a pathway by which glucose regulates β-catenin signalling via the cAMP/protein kinase A pathway in β-cell models

Includes: Supplementary data

Articles

The A-kinase-anchoring protein AKAP-Lbc facilitates cardioprotective PKA phosphorylation of Hsp20 on Ser 16

Helen V. Edwards, John D. Scott, George S. Baillie

Biochem J (2012) 446 (3): 437–443.

https://doi.org/10.1042/BJ20120570

Published: 28 August 2012

... require its phosphorylation by PKA (cAMP-dependent protein kinase) on Ser 16 . Although the extracellular stimuli that promote cAMP-responsive phosphorylation of Hsp20 are well known, less is understood about the molecular processes that regulate this modification. AKAPs (A-kinase-anchoring proteins...

View article titled, The A-kinase-anchoring protein AKAP-Lbc facilitates cardioprotective PKA phosphorylation of Hsp20 on Ser 16

Articles

The AMPK-related kinase SIK2 is regulated by cAMP via phosphorylation at Ser 358 in adipocytes

Emma Henriksson, Helena A. Jones, Kashyap Patel, Mark Peggie, Nicholas Morrice, Kei Sakamoto, Olga Göransson

Biochem J (2012) 444 (3): 503–514.

https://doi.org/10.1042/BJ20111932

Published: 29 May 2012

... in adipocytes in response to cellular stimuli with relevance for adipocyte function and/or AMPK signalling. None of the treatments, including insulin, cAMP inducers or AICAR (5-amino-4-imidazolecarboxamide riboside), affected SIK2 activity towards peptide or protein substrates in vitro . However, stimulation...

View article titled, The AMPK-related kinase SIK2 is regulated by cAMP via phosphorylation at Ser 358 in adipocytes

Articles

Interaction with receptor for activated C-kinase 1 (RACK1) sensitizes the phosphodiesterase PDE4D5 towards hydrolysis of cAMP and activation by protein kinase C

Rebecca J. Bird, George S. Baillie, Stephen J. Yarwood

Biochem J (2010) 432 (1): 207–219.

https://doi.org/10.1042/BJ20101010

Published: 25 October 2010

...Rebecca J. Bird; George S. Baillie; Stephen J. Yarwood We have previously identified the PKC (protein kinase C)-anchoring protein RACK1 (receptor for activated C-kinase 1), as a specific binding partner for the cAMP-specific phosphodiesterase PDE4D5, suggesting a potential site for cross-talk...

View article titled, Interaction with receptor for activated C-kinase 1 (RACK1) sensitizes the phosphodiesterase PDE4D5 towards hydrolysis of cAMP and activation by protein kinase C

Includes: Supplementary data

Articles

Inhibition of the mitochondrial permeability transition by protein kinase A in rat liver mitochondria and hepatocytes

Peter Pediaditakis, Jae-Sung Kim, Lihua He, Xun Zhang, Lee M. Graves, John J. Lemasters

Biochem J (2010) 431 (3): 411–421.

https://doi.org/10.1042/BJ20091741

Published: 11 October 2010

... of hepatic cytosol in combination with cyclic nucleotides to delay onset of the calcium-induced MPT was evaluated in isolated rat liver mitochondria. Liver cytosol plus cGMP or cAMP dose-dependently inhibited the MPT, required ATP hydrolysis for inhibition and did not inhibit mitochondrial calcium uptake...

View article titled, Inhibition of the mitochondrial permeability transition by protein kinase A in rat liver mitochondria and hepatocytes

Articles

Selective SUMO modification of cAMP-specific phosphodiesterase-4D5 (PDE4D5) regulates the functional consequences of phosphorylation by PKA and ERK

Xiang Li, Suryakiran Vadrevu, Allan Dunlop, Jon Day, Noopur Advant, Jessica Troeger, Enno Klussmann, Ellis Jaffrey, Ron T. Hay, David R. Adams, Miles D. Houslay, George S. Baillie

Biochem J (2010) 428 (1): 55–65.

https://doi.org/10.1042/BJ20091672

Published: 28 April 2010

...Xiang Li; Suryakiran Vadrevu; Allan Dunlop; Jon Day; Noopur Advant; Jessica Troeger; Enno Klussmann; Ellis Jaffrey; Ron T. Hay; David R. Adams; Miles D. Houslay; George S. Baillie Enzymes from the PDE (phosphodiesterase) 4 cAMP-specific PDE family are crucial for the maintenance...

View article titled, Selective SUMO modification of cAMP-specific phosphodiesterase-4D5 (PDE4D5) regulates the functional consequences of phosphorylation by PKA and ERK

Articles

Design of proteolytically stable RI-anchoring disruptor peptidomimetics for in vivo studies of anchored type I protein kinase A-mediated signalling

Eirik A. Torheim, Elisabeth Jarnæss, Birgitte Lygren, Kjetil Taskén

Biochem J (2009) 424 (1): 69–78.

https://doi.org/10.1042/BJ20090933

Published: 23 October 2009

... synapse of T-cells. This increases immune reactivity by reducing the threshold for activation and may prove a feasible approach for improving immune function in patients with cAMP-mediated T-cell dysfunction. However, the use of RIAD in biological systems is restricted by its susceptibility to enzymatic...

View article titled, Design of proteolytically stable RI-anchoring disruptor peptidomimetics for in vivo studies of anchored type I protein kinase A-mediated signalling

Articles

Binding of cyclic nucleotides to phosphodiesterase 10A and 11A GAF domains does not stimulate catalytic activity

Karina Matthiesen, Jacob Nielsen

Biochem J (2009) 423 (3): 401–409.

https://doi.org/10.1042/BJ20090982

Published: 12 October 2009

... described PDEs and it has been suggested that their GAF domains bind to cAMP and cGMP respectively. We have developed a scintillation proximity-based assay to directly measure cyclic nucleotide binding to the PDE2A, PDE10A and PDE11A GAF domains, and in the present study we demonstrate binding of cyclic...

View article titled, Binding of cyclic nucleotides to phosphodiesterase 10A and 11A GAF domains does not stimulate catalytic activity

Articles

Structure–function relationships in Escherichia coli adenylate cyclase

Jürgen U. Linder

Biochem J (2008) 415 (3): 449–454.

https://doi.org/10.1042/BJ20080350

Published: 15 October 2008

..., which has been studied intensively in the last few decades [ 12 – 14 ]. When cells are grown in a mixture of the preferred carbon source glucose and a less preferred source such as lactose, only glucose is used, and the cAMP levels are low. Once glucose is scarce, AC is activated and the cAMP levels...

View article titled, Structure–function relationships in Escherichia coli adenylate cyclase

Includes: Supplementary data

Articles

Human PDE4A8, a novel brain-expressed PDE4 cAMP-specific phosphodiesterase that has undergone rapid evolutionary change

Kirsty F. Mackenzie, Emma C. Topping, Bozena Bugaj-Gaweda, Chengjun Deng, York-Fong Cheung, Aileen E. Olsen, Cecil R. Stockard, Lisa High Mitchell, George S. Baillie, William E. Grizzle, Michael De Vivo, Miles D. Houslay, Daguang Wang, Graeme B. Bolger

Biochem J (2008) 411 (2): 361–369.

https://doi.org/10.1042/BJ20071251

Published: 27 March 2008

... (phosphodiesterase 4 isoform A8), a new human cAMP-specific PDE4 isoform encoded by the PDE4A gene. PDE4A8 has a novel N-terminal region of 85 amino acids that differs from those of the related ‘long’ PDE4A4, PDE4A10 and PDE4A11 isoforms. The human PDE4A8 N-terminal region has diverged substantially from...

View article titled, Human PDE4A8, a novel brain-expressed PDE4 cAMP-specific phosphodiesterase that has undergone rapid evolutionary change

Articles

Regulation of type V adenylate cyclase by Ric8a, a guanine nucleotide exchange factor

Shyi-Chyi Wang, Hsing-Lin Lai, Yi-Ting Chiu, Ren Ou, Chuen-Lin Huang, Yijuang Chern

Biochem J (2007) 406 (3): 383–388.

https://doi.org/10.1042/BJ20070512

Published: 29 August 2007

... the rat striatum or 30 μg of the purified recombinant N-terminus of AC5 (AC5N 1–215 ) at 4 °C for 1 h, washed five times with ice-cold harvest buffer, and resolved by SDS/PAGE and Western blot analysis. cAMP guanine nucleotide exchange factor (GEF) Ric8a type V adenylate cyclase (AC5) ACs...

View article titled, Regulation of type V adenylate cyclase by Ric8a, a guanine nucleotide exchange factor

Articles

Fluvastatin inhibits regulated secretion of endothelial cell von Willebrand factor in response to diverse secretagogues

Richard J. Fish, Hong Yang, Christelle Viglino, Raoul Schorer, Sylvie Dunoyer-Geindre, Egbert K. O. Kruithof

Biochem J (2007) 405 (3): 597–604.

https://doi.org/10.1042/BJ20070404

Published: 13 July 2007

...Richard J. Fish; Hong Yang; Christelle Viglino; Raoul Schorer; Sylvie Dunoyer-Geindre; Egbert K. O. Kruithof Regulated secretion of EC (endothelial cell) vWF (von Willebrand factor) is part of the haemostatic response. It occurs in response to secretagogues that raise intracellular calcium or cAMP...

View article titled, Fluvastatin inhibits regulated secretion of endothelial cell von Willebrand factor in response to diverse secretagogues

Includes: Supplementary data

Articles

Activation mechanisms for the cystic fibrosis transmembrane conductance regulator protein involve direct binding of cAMP

Malcolm M. C. Pereira, Jody Parker, Fiona L. L. Stratford, Margaret McPherson, Robert L. Dormer

Biochem J (2007) 405 (1): 181–189.

https://doi.org/10.1042/BJ20061879

Published: 13 June 2007

... mechanisms. Other CFTR functions such as regulation of exocytotic protein secretion are also activated by cyclic nucleotide elevating agents. A soluble protein comprising the first NBD (nucleotide-binding domain) and R-domain of CFTR (NBD1–R) was synthesized to determine directly whether CFTR binds cAMP...

View article titled, Activation mechanisms for the cystic fibrosis transmembrane conductance regulator protein involve direct binding of cAMP

Articles

Mapping binding sites for the PDE4D5 cAMP-specific phosphodiesterase to the N- and C-domains of β-arrestin using spot-immobilized peptide arrays

George S. Baillie, David R. Adams, Narinder Bhari, Thomas M. Houslay, Suryakiran Vadrevu, Dong Meng, Xiang Li, Allan Dunlop, Graeme Milligan, Graeme B. Bolger, Enno Klussmann, Miles D. Houslay

Biochem J (2007) 404 (1): 71–80.

https://doi.org/10.1042/BJ20070005

Published: 26 April 2007

... (family 4 cAMP-specific phosphodiesterase, subfamily D, isoform 5) can be recruited in complex with β-arrestin, whereupon it regulates PKA (cAMP-dependent protein kinase) phosphorylation of the β 2 -AR. In the present study, we have used novel technology, employing a library of overlapping peptides (25...

View article titled, Mapping binding sites for the PDE4D5 cAMP-specific phosphodiesterase to the N- and C-domains of β-arrestin using spot-immobilized peptide arrays

Articles

Regulation of adiponectin and leptin secretion and expression by insulin through a PI3K-PDE3B dependent mechanism in rat primary adipocytes

Li Cong, Ke Chen, Ji Li, Ping Gao, Qiang Li, Shuhua Mi, Xin Wu, Allan Z. Zhao

Biochem J (2007) 403 (3): 519–525.

https://doi.org/10.1042/BJ20061478

Published: 12 April 2007

..., such an inhibitory effect could be blocked by insulin. The opposing effects of isoprenaline and insulin could be explained by differential regulation of intracellular cAMP levels, since cAMP analogues suppressed adiponectin secretion and expression in a fashion similar to isoprenaline, and insulin blocked...

View article titled, Regulation of adiponectin and leptin secretion and expression by insulin through a PI3K-PDE3B dependent mechanism in rat primary adipocytes

Includes: Supplementary data

Articles

Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP

Sonya Bader, Arjan Kortholt, Peter J. M. Van Haastert

Biochem J (2007) 402 (1): 153–161.

https://doi.org/10.1042/BJ20061153

Published: 25 January 2007

... nucleotides: (i) extracellular cAMP that induces chemotaxis during aggregation and differentiation in slugs; (ii) intracellular cAMP that mediates development; and (iii) intracellular cGMP that mediates chemotaxis. It appears that each cyclic nucleotide pool is degraded by a combination of enzymes that have...

View article titled, Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP

Articles

Pharmacological profiling of the Dictyostelium adenylate cyclases ACA, ACB and ACG

Elisa Alvarez-Curto, Karin E. Weening, Pauline Schaap

Biochem J (2007) 401 (1): 309–316.

https://doi.org/10.1042/BJ20060880

Published: 11 December 2006

...Elisa Alvarez-Curto; Karin E. Weening; Pauline Schaap Intracellular and secreted cAMPs play crucial roles in controlling cell movement and gene regulation throughout development of the social amoeba Dictyostelium discoideum . cAMP is produced by three structurally distinct ACs (adenylate cyclases...

View article titled, Pharmacological profiling of the Dictyostelium adenylate cyclases ACA, ACB and ACG

Articles

Characterization of a novel cAMP-binding, cAMP-specific cyclic nucleotide phosphodiesterase (TcrPDEB1) from Trypanosoma cruzi

Rocío Díaz-Benjumea, Sunil Laxman, Thomas R. Hinds, Joseph A. Beavo, Ana Rascón

Biochem J (2006) 399 (2): 305–314.

https://doi.org/10.1042/BJ20060757

Published: 27 September 2006

...Rocío Díaz-Benjumea; Sunil Laxman; Thomas R. Hinds; Joseph A. Beavo; Ana Rascón Trypanosoma cruzi , the causative agent of Chagas disease, encodes a number of different cAMP-specific PDE (phosphodiesterase) families. Here we report the identification and characterization of TcrPDEB1 and its...

View article titled, Characterization of a novel cAMP-binding, cAMP-specific cyclic nucleotide phosphodiesterase (TcrPDEB1) from Trypanosoma cruzi

Includes: Supplementary data

Articles

Recombinant human hyaluronan synthase 3 is phosphorylated in mammalian cells

Brian J. Goentzel, Paul H. Weigel, Robert A. Steinberg

Biochem J (2006) 396 (2): 347–354.

https://doi.org/10.1042/BJ20051782

Published: 15 May 2006

... cells, we show that HAS3 is serine-phosphorylated and that this phosphorylation can be enhanced by a number of effectors – most significantly by a membrane-permeable analogue of cAMP. By employing a novel FLAG-tagged phosphorylated reference protein derived from EGFP (enhanced green fluorescent protein...

View article titled, Recombinant human hyaluronan synthase 3 is phosphorylated in mammalian cells

Articles

Regulation of prokaryotic adenylyl cyclases by CO 2

Arne Hammer, David R. W. Hodgson, Martin J. Cann

Biochem J (2006) 396 (2): 215–218.

https://doi.org/10.1042/BJ20060372

Published: 15 May 2006

...) supported this work. 1 To whom correspondence should be addressed (email [email protected] ). 8 3 2006 28 3 2006 30 3 2006 30 3 2006 The Biochemical Society, London 2006 adenylyl cyclase (adenyl cyclase, adenylate cyclase) bicarbonate (HCO 3 − ) cAMP...

View article titled, Regulation of prokaryotic adenylyl cyclases by CO 2

Articles

Gene expression profiling of mice with genetically modified muscle glycogen content

Gretchen E. Parker, Bartholomew A. Pederson, Mariko Obayashi, Jill M. Schroeder, Robert A. Harris, Peter J. Roach

Biochem J (2006) 395 (1): 137–145.

https://doi.org/10.1042/BJ20051456

Published: 15 March 2006

..., London 2006 branched-chain amino acid oxidation cAMP glyco-gen glycogen synthase phosphatase inhibitor 1 (I-1) Wnt Glycogen is a branched polymer of glucose and the principal reserve of carbohydrate in mammals, with the largest deposits being in liver and muscle [ 1 ]. Conversion...

View article titled, Gene expression profiling of mice with genetically modified muscle glycogen content

Includes: Supplementary data

Articles

Cyclic nucleotide phosphodiesterases as targets for treatment of haematological malignancies

Adam Lerner, Paul M. Epstein

Biochem J (2006) 393 (1): 21–41.

https://doi.org/10.1042/BJ20051368

Published: 12 December 2005

...Adam Lerner; Paul M. Epstein The cAMP signalling pathway has emerged as a key regulator of haematopoietic cell proliferation, differentiation and apoptosis. In parallel, general understanding of the biology of cyclic nucleotide PDEs (phosphodiesterases) has advanced considerably, revealing...

View article titled, Cyclic nucleotide phosphodiesterases as targets for treatment of haematological malignancies

Articles

Phosphodiesterase 3A binds to 14-3-3 proteins in response to PMA-induced phosphorylation of Ser 428

Mercedes Pozuelo Rubio, David G. Campbell, Nicholas A. Morrice, Carol Mackintosh

Biochem J (2005) 392 (1): 163–172.

https://doi.org/10.1042/BJ20051103

Published: 08 November 2005

... of PDE3A was phosphorylated in an H-89-sensitive response to forskolin, indicative of phosphorylation by PKA (cAMP-dependent protein kinase), but phosphorylation at this site did not stimulate 14-3-3 binding. Thus 14-3-3 proteins can discriminate between sites in a region of multisite phosphorylation...

View article titled, Phosphodiesterase 3A binds to 14-3-3 proteins in response to PMA-induced phosphorylation of Ser 428

Includes: Supplementary data

Articles

Anti-inflammatory potential of CB1-mediated cAMP elevation in mast cells

Andrea L. SMALL-HOWARD, Lori M. N. SHIMODA, Chaker N. ADRA, Helen TURNER

Biochem J (2005) 388 (2): 465–473.

https://doi.org/10.1042/BJ20041682

Published: 24 May 2005

... cell activation. Our results show that CB1 and CB2 mediate diametrically opposed effects on cAMP levels in mast cells. The observed long-term stimulation of cAMP levels by the Gα i/o -coupled CB1 is paradoxical, and our results indicate that it may be attributed to CB1-mediated transcriptional...

View article titled, Anti-inflammatory potential of CB1-mediated cAMP elevation in mast cells

Articles

Biosynthesis of agmatine in isolated mitochondria and perfused rat liver: studies with 15 N-labelled arginine

Oksana HORYN, Bohdan LUHOVYY, Adam LAZAROW, Yevgeny DAIKHIN, Ilana NISSIM, Marc YUDKOFF, Itzhak NISSIM

Biochem J (2005) 388 (2): 419–425.

https://doi.org/10.1042/BJ20041260

Published: 24 May 2005

... to address this question and to determine the flux through the ADC reaction in isolated mitochondria obtained from rat liver. In addition, liver perfusion system was used to examine a possible action of insulin, glucagon or cAMP on a flux through the ADC reaction. In mitochondria and liver perfusion, 15 N...

View article titled, Biosynthesis of agmatine in isolated mitochondria and perfused rat liver: studies with 15 N-labelled arginine

Articles

Characterization of phylogenetically distant members of the adenylate cyclase family from mycobacteria: Rv1647 from Mycobacterium tuberculosis and its orthologue ML1399 from M. leprae

Avinash R. SHENOY, Nandini P. SREENATH, Mohana MAHALINGAM, Sandhya S. VISWESWARIAH

Biochem J (2005) 387 (2): 541–551.

https://doi.org/10.1042/BJ20041040

Published: 05 April 2005

... and perhaps the pathology of these medically important bacteria. adenylate cyclase cAMP enzyme Mycobacterium leprae Mycobacterium tuberculosis phylogenetic analysis The sequence data for the Rv1647 gene from Mycobacterium smegmatis will appear in the DDBJ, EMBL, GenBank® and GSDB...

View article titled, Characterization of phylogenetically distant members of the adenylate cyclase family from mycobacteria: Rv1647 from Mycobacterium tuberculosis and its orthologue ML1399 from M. leprae

Articles

Functional studies of the 5′-untranslated region of human 5-HT 4 receptor mRNA

Marjorie MAILLET, Monique GASTINEAU, Pascal BOCHET, Marie-Liesse ASSELIN-LABAT, Eric MOREL, Jean-Noël LAVERRIÈRE, Anne-Marie LOMPRÉ, Rodolphe FISCHMEISTER, Frank LEZOUALC'H

Biochem J (2005) 387 (2): 463–471.

https://doi.org/10.1042/BJ20040744

Published: 05 April 2005

... 2005 cAMP 5-hydroxytryptamine (serotonin) mRNA Nkx2.5 transcriptional regulation 5′-untranslated region Serotonin (5-HT, 5-hydroxytryptamine) is a major neurotransmitter in both the central nervous system and peripheral tissues. Of the 14 mammalian 5-HT receptor subtypes identified so...

View article titled, Functional studies of the 5′-untranslated region of human 5-HT 4 receptor mRNA

Articles

Regulation of adenylate cyclase type VIII splice variants by acute and chronic G i/o -coupled receptor activation

Debora STEINER, Tomer AVIDOR-REISS, Ester SCHALLMACH, Elena BUTOVSKY, Nirit LEV, Zvi VOGEL

Biochem J (2005) 386 (2): 341–348.

https://doi.org/10.1042/BJ20041670

Published: 22 February 2005

... to be inhibited by acute agonist exposure, agonist withdrawal after prolonged treatment led to a similar superactivation of all three splice variants, with no significant change in AC-VIII expression. AC-VIII superactivation was not affected by pre-incubation with a cell permeable cAMP analogue, indicating...

View article titled, Regulation of adenylate cyclase type VIII splice variants by acute and chronic G i/o -coupled receptor activation

Articles

Inhibition of Rac activation as a mechanism for negative regulation of actin cytoskeletal reorganization and cell motility by cAMP

Shin-ya NAGASAWA, Noriko TAKUWA, Naotoshi SUGIMOTO, Hiroshi MABUCHI, Yoh TAKUWA

Biochem J (2005) 385 (3): 737–744.

https://doi.org/10.1042/BJ20041060

Published: 24 January 2005

...Shin-ya NAGASAWA; Noriko TAKUWA; Naotoshi SUGIMOTO; Hiroshi MABUCHI; Yoh TAKUWA cAMP has been found to play a role in mediating the negative regulation of cell motility, although its underlying molecular mechanism remains poorly understood. By using CHO (Chinese-hamster ovary) cells that express...

View article titled, Inhibition of Rac activation as a mechanism for negative regulation of actin cytoskeletal reorganization and cell motility by cAMP

Articles

Cyclo-oxygenase type 2-dependent prostaglandin E 2 secretion is involved in retrovirus-induced T-cell dysfunction in mice

Souad RAHMOUNI, Einar Martin AANDAHL, Btissam NAYJIB, Mustapha ZEDDOU, Sandra GIANNINI, Myriam VERLAET, Roland GREIMERS, Jaques BONIVER, Kjetil TASKEN, Michel MOUTSCHEN

Biochem J (2004) 384 (3): 469–476.

https://doi.org/10.1042/BJ20031859

Published: 07 December 2004

... immunodeficiency and T-cell anergy combined with a lymphoproliferative disease affecting both B- and T-cells. Hyperactivation of the cAMP-protein kinase A pathway is involved in the T-cell dysfunction of MAIDS and HIV by inhibiting T-cell activation through the T-cell receptor. In the present study, we show...

View article titled, Cyclo-oxygenase type 2-dependent prostaglandin E 2 secretion is involved in retrovirus-induced T-cell dysfunction in mice

Articles

Noradrenaline represses PPAR (peroxisome-proliferator-activated receptor) γ2 gene expression in brown adipocytes: intracellular signalling and effects on PPARγ2 and PPARγ1 protein levels

Eva M. LINDGREN, Ronni NIELSEN, Natasa PETROVIC, Anders JACOBSSON, Susanne MANDRUP, Barbara CANNON, Jan NEDERGAARD

Biochem J (2004) 382 (2): 597–606.

https://doi.org/10.1042/BJ20031622

Published: 24 August 2004

... and differentiating cells, with a lagtime of 1 h and lasting up to 4 h, after which expression gradually recovered. The down-regulation was β-adrenoceptor-induced and intracellularly mediated via cAMP and protein kinase A; the signalling pathway did not involve phosphoinositide 3-kinase, Src, p38 mitogen-activated...

View article titled, Noradrenaline represses PPAR (peroxisome-proliferator-activated receptor) γ2 gene expression in brown adipocytes: intracellular signalling and effects on PPARγ2 and PPARγ1 protein levels

Includes: Supplementary data

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