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Keywords: cGMP-dependent protein kinase (cGK)
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Articles
Design of fluorescence resonance energy transfer (FRET)-based cGMP indicators: a systematic approach
Available to PurchaseMichael Russwurm, Florian Mullershausen, Andreas Friebe, Ronald Jäger, Corina Russwurm, Doris Koesling
Journal:
Biochemical Journal
Biochem J (2007) 407 (1): 69–77.
Published: 12 September 2007
... protein kinase (cGK) fluorescence resonance energy transfer (FRET) phosphodiesterase (PDE) The indicators were transiently transfected into HEK-293 (human embryonic kidney) cells using Fugene6, according to the manufacturer's (Roche) instructions. Transfected cells (1×10 7 cells) were harvested...
Includes: Multimedia, Supplementary data
