...://pymol.org ). calcium calcium binding CBL chlamydomonas EF-hand motif signaling Chlamydomonas reinhardtii wild type strain CC-124 was procured from Chlamydomonas resource center ( www.chlamycollection.org ). Culture was grown using TAP (tris-acetate-phosphate media, pH 7.4...

... regulates energy metabolism through its sensitivity to adenine nucleotides, NADH and Ca 2+ . In the present study, we show definitively that the E1 subunit is the site of action for these regulators, and identify the Ca 2+ -binding site. calcium binding citrate cycle energy metabolism E1 subunit...

...Marie Boudsocq; Marie-Jo Droillard; Leslie Regad; Christiane Laurière CDPKs (calcium-dependent protein kinases), which contain both calmodulin-like calcium binding and serine/threonine protein kinase domains, are only present in plants and some protozoans. Upon activation by a stimulus...

... or [email protected] ). 21 1 2008 8 4 2008 9 4 2008 9 4 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 calcium binding calsequestrin circular dichroism fluorescence protein stability proteolysis The homogeneity of CASQ2 proteins...

... Antarctic bacterium antifreeze protein calcium binding β-helix Marinomonas primoryensis repeats-in-toxin proteins (RTX proteins) Since their discovery over 30 years ago in Antarctic fish [ 1 ], antifreeze proteins (AFPs) have been found in a wide array of organisms that inhabit freezing...

... 6 2006 22 8 2006 30 8 2006 30 8 2006 The Biochemical Society, London 2007 calcium binding conformational change EF-hand 25 kDa synaptosome-associated protein (SNAP-25) secretagogin sensor Secretagogin is a recently described protein that is expressed...

... for MMP-12 activity and the properties of the different Ca 2+ sites, the activity as a function of [Ca 2+ ] and the effect of pH was investigated. The enzymatic activity was directly correlated to calcium binding and a Langmuir isotherm for three binding sites described the activity as a function of [Ca 2...

... to acidic phospholipids at high calcium concentrations. We show for the first time that myristoylation of annexin A13b enables the direct binding to phosphatidylcholine, raft-like liposomes and acidic phospholipids in a calcium-independent manner. The conformational switch induced by calcium binding, from...

...-mail [email protected] ) 4 6 2003 18 8 2003 28 8 2003 28 8 2003 The Biochemical Society, London ©2003 2003 calcium binding EF hand enzyme oligomerization neutral trehalase activity Schizosaccharomyces pombe Abbreviations used: GST, glutathione S-transferase; Ha...

... reveals two main secondary-structure contributions, α-helix and random coil (approx. 30% each), corresponding mainly to the annexin C-terminal tetrad and the N-terminus respectively. On calcium binding, an increase in α-helix and a decrease in random coil are detected. Fluorescence spectroscopy reveals...

... a kinetic denaturation mechanism where the calcium-depleted amylase denatures irreversibly at low temperature and if calcium ions are in excess, the amylase denatures irreversibly at high temperatures. The two denaturation reactions are coupled with the calcium-binding equilibrium between calcium-bound...

... modulator and a peptidase. Key words: acidic domain, calcium binding, domain deletion, M16 family. 47% identity in the E 200 amino acid catalytic region conserved among the family members [15]. NRDc was the first reported M16 member to present an insertion in the catalytic region. This insertion...

... that this protease has a limited substrate specificity, so this application was not explored further. Key words: calcium binding, heat stable, specificity. an alternative thermostable Cadependent protease was sought that was more commercially viable to produce. Thus Bacillus st. Ak.1 protease was targeted...

... Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, U.K. 2 To whom correspondence should be addressed (e-mail [email protected]). 29 11 1999 16 2 2000 10 3 2000 The Biochemical Society, London © 2000 2000 calcium binding co-operativity...