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1-14 of 14
Keywords: calcium binding
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Articles
Genome-wide identification and biochemical characterization of calcineurin B-like calcium sensor proteins in Chlamydomonas reinhardtii
Available to PurchaseManoj Kumar, Komal Sharma, Akhilesh K. Yadav, Kajal Kanchan, Madhu Baghel, Suneel Kateriya, Girdhar K. Pandey
Journal:
Biochemical Journal
Biochem J (2020) 477 (10): 1879–1892.
Published: 28 May 2020
...://pymol.org ). calcium calcium binding CBL chlamydomonas EF-hand motif signaling Chlamydomonas reinhardtii wild type strain CC-124 was procured from Chlamydomonas resource center ( www.chlamycollection.org ). Culture was grown using TAP (tris-acetate-phosphate media, pH 7.4...
Includes: Supplementary data
Articles
Studies on the regulation of the human E1 subunit of the 2-oxoglutarate dehydrogenase complex, including the identification of a novel calcium-binding site
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2014) 459 (2): 369–381.
Published: 28 March 2014
... regulates energy metabolism through its sensitivity to adenine nucleotides, NADH and Ca 2+ . In the present study, we show definitively that the E1 subunit is the site of action for these regulators, and identify the Ca 2+ -binding site. calcium binding citrate cycle energy metabolism E1 subunit...
Includes: Supplementary data
Articles
Characterization of Arabidopsis calcium-dependent protein kinases: activated or not by calcium?
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2012) 447 (2): 291–299.
Published: 26 September 2012
...Marie Boudsocq; Marie-Jo Droillard; Leslie Regad; Christiane Laurière CDPKs (calcium-dependent protein kinases), which contain both calmodulin-like calcium binding and serine/threonine protein kinase domains, are only present in plants and some protozoans. Upon activation by a stimulus...
Includes: Supplementary data
Articles
Catecholaminergic polymorphic ventricular tachycardia-related mutations R33Q and L167H alter calcium sensitivity of human cardiac calsequestrin
Available to PurchaseGiorgia Valle, Daniela Galla, Alessandra Nori, Silvia G. Priori, Sandor Gyorke, Vincenzo de Filippis, Pompeo Volpe
Journal:
Biochemical Journal
Biochem J (2008) 413 (2): 291–303.
Published: 26 June 2008
... or [email protected] ). 21 1 2008 8 4 2008 9 4 2008 9 4 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 calcium binding calsequestrin circular dichroism fluorescence protein stability proteolysis The homogeneity of CASQ2 proteins...
Articles
A Ca 2+ -dependent bacterial antifreeze protein domain has a novel β-helical ice-binding fold
Available to PurchaseChristopher P. Garnham, Jack A. Gilbert, Christopher P. Hartman, Robert L. Campbell, Johanna Laybourn-Parry, Peter L. Davies
Journal:
Biochemical Journal
Biochem J (2008) 411 (1): 171–180.
Published: 13 March 2008
... Antarctic bacterium antifreeze protein calcium binding β-helix Marinomonas primoryensis repeats-in-toxin proteins (RTX proteins) Since their discovery over 30 years ago in Antarctic fish [ 1 ], antifreeze proteins (AFPs) have been found in a wide array of organisms that inhabit freezing...
Articles
Binding of calcium ions and SNAP-25 to the hexa EF-hand protein secretagogin
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2007) 401 (1): 353–363.
Published: 11 December 2006
... 6 2006 22 8 2006 30 8 2006 30 8 2006 The Biochemical Society, London 2007 calcium binding conformational change EF-hand 25 kDa synaptosome-associated protein (SNAP-25) secretagogin sensor Secretagogin is a recently described protein that is expressed...
Includes: Supplementary data
Articles
Characterization of Ca 2+ interactions with matrix metallopeptidase-12: implications for matrix metallopeptidase regulation
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2006) 398 (3): 393–398.
Published: 29 August 2006
... for MMP-12 activity and the properties of the different Ca 2+ sites, the activity as a function of [Ca 2+ ] and the effect of pH was investigated. The enzymatic activity was directly correlated to calcium binding and a Langmuir isotherm for three binding sites described the activity as a function of [Ca 2...
Articles
Structure–function relationship in annexin A13, the founder member of the vertebrate family of annexins
Available to PurchaseJavier Turnay, Emilio Lecona, Sara Fernández-Lizarbe, Ana Guzmán-Aránguez, María Pilar Fernández, Nieves Olmo, Ma Antonia Lizarbe
Journal:
Biochemical Journal
Biochem J (2005) 389 (3): 899–911.
Published: 26 July 2005
... to acidic phospholipids at high calcium concentrations. We show for the first time that myristoylation of annexin A13b enables the direct binding to phosphatidylcholine, raft-like liposomes and acidic phospholipids in a calcium-independent manner. The conformational switch induced by calcium binding, from...
Articles
A role for calcium in the regulation of neutral trehalase activity in the fission yeast Schizosaccharomyces pombe
Available to PurchaseAlejandro FRANCO, Teresa SOTO, Jero VICENTE-SOLER, Vanessa PAREDES, Marisa MADRID, Mariano GACTO, José CANSADO
Journal:
Biochemical Journal
Biochem J (2003) 376 (1): 209–217.
Published: 15 November 2003
...-mail [email protected] ) 4 6 2003 18 8 2003 28 8 2003 28 8 2003 The Biochemical Society, London ©2003 2003 calcium binding EF hand enzyme oligomerization neutral trehalase activity Schizosaccharomyces pombe Abbreviations used: GST, glutathione S-transferase; Ha...
Articles
Structural and functional characterization of recombinant mouse annexin A11: influence of calcium binding
Available to PurchaseEmilio LECONA, Javier TURNAY, Nieves OLMO, Ana GUZMÁN-ARÁNGUEZ, Reginald O. MORGAN, Maria-Pilar FERNANDEZ, Ma Antonia LIZARBE
Journal:
Biochemical Journal
Biochem J (2003) 373 (2): 437–449.
Published: 15 July 2003
... reveals two main secondary-structure contributions, α-helix and random coil (approx. 30% each), corresponding mainly to the annexin C-terminal tetrad and the N-terminus respectively. On calcium binding, an increase in α-helix and a decrease in random coil are detected. Fluorescence spectroscopy reveals...
Articles
Effect of calcium ions on the irreversible denaturation of a recombinant Bacillus halmapalus alpha-amylase: a calorimetric investigation
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2003) 373 (2): 337–343.
Published: 15 July 2003
... a kinetic denaturation mechanism where the calcium-depleted amylase denatures irreversibly at low temperature and if calcium ions are in excess, the amylase denatures irreversibly at high temperatures. The two denaturation reactions are coupled with the calcium-binding equilibrium between calcium-bound...
Articles
The metalloendopeptidase nardilysin (NRDc) is potently inhibited by heparin-binding epidermal growth factor-like growth factor (HB-EGF)
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2002) 367 (1): 229–238.
Published: 01 October 2002
... modulator and a peptidase. Key words: acidic domain, calcium binding, domain deletion, M16 family. 47% identity in the E 200 amino acid catalytic region conserved among the family members [15]. NRDc was the first reported M16 member to present an insertion in the catalytic region. This insertion...
Articles
Purification and characterization of Ak.1 protease, a thermostable subtilisin with a disulphide bond in the substrate-binding cleft
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2000) 350 (1): 321–328.
Published: 09 August 2000
... that this protease has a limited substrate specificity, so this application was not explored further. Key words: calcium binding, heat stable, specificity. an alternative thermostable Cadependent protease was sought that was more commercially viable to produce. Thus Bacillus st. Ak.1 protease was targeted...
Articles
Roles of individual EF-hands in the activation of m-calpain by calcium
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2000) 348 (1): 37–43.
Published: 09 May 2000
... Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, U.K. 2 To whom correspondence should be addressed (e-mail [email protected]). 29 11 1999 16 2 2000 10 3 2000 The Biochemical Society, London © 2000 2000 calcium binding co-operativity...