... due to the polymeric and polydisperse nature of the substrate. A novel activity assay based on HPCE (high performance capillary electrophoresis), in conjunction with a defined low-molecular-mass XGO {XG oligosaccharide; (XXXGXXXG, where G=Glcβ1,4- and X=[Xylα1,6]Glcβ1,4-)} as the glycosyl donor...

... ]. Arabidopsis thaliana autophosphorylation calmodulin capillary electrophoresis Ca 2+ -dependent protein-kinase (CDPK)-related protein kinase (CRK) The Biochemical Society, London 2004 10 12 2003 4 6 2004 15 6 2004 15 6 2004 The nucleotide sequence data reported have...

... of activity. Furthermore, metal-substituted PTEs display differences in catalytic properties. A prerequisite for engineering highly efficient mutants of PTE is to improve their thermostability. Isoelectric focusing, capillary electrophoresis and steady-state kinetics analysis were used to determine...

...) capillary electrophoresis dissociation constant phosphorylation tobacco Abbreviations used: CaM, calmodulin; CaMK, Ca 2+ /CaM-dependent protein kinase; CBK, CaM-binding protein kinase; CCaMK, chimaeric CaMK; CDPK, Ca 2+ -dependent protein kinase; CRK, CDPK-related kinase; DTT, dithiothreitol...

.... Using a range of chemical degradations, NMR experiments and capillary electrophoresis analysis, AsuLAM was revealed as an original structure. The mannosyl-phosphatidyl- myo -inositol anchor exhibits a single acyl-form, characterized by a diacylated glycerol moiety, and contains, as one of the main fatty...

... correspondence should be addressed (e-mail [email protected] ). 20 5 2002 3 7 2002 3 7 2002 The Biochemical Society, London ©2002 2002 capillary electrophoresis pectin digests pectin methylesterase polygalacturonase polysaccharide-degrading enzymes...