... deacetylase isoform 5) and thereby de-repression of MEF2 (myocyte enhancer factor 2) transcription factor activity. In the present study we identify FHL1 (four-and-a-half LIM domains protein 1) and FHL2 as novel binding partners for PKD in cardiac myocytes. This was confirmed by pull-down assays using...

...Tsutomu Nakada; Bernhard E. Flucher; Toshihide Kashihara; Xiaona Sheng; Toshihide Shibazaki; Miwa Horiuchi-Hirose; Simmon Gomi; Masamichi Hirose; Mitsuhiko Yamada In cardiac myocytes, LTCCs (L-type calcium channels) form a functional signalling complex with ryanodine receptors at the JM (junctional...

... Dako, and Pierce horseradish peroxidase-conjugated anti-sheep antibodies were from ThermoFisher (catalogue number 31627). The Alexa Fluor® 488-conjugated anti-mouse secondary antibody (catalogue number A11001) and Texas Red–phalloidin (catalogue number T7471) were from Invitrogen. cardiac myocyte...

...Robert S. Haworth; Semjidmaa Dashnyam; Metin Avkiran In cardiac myocytes, sustained (3 min) intracellular acidosis activates the ERK1/2 (extracellular-signal-regulated kinase 1/2) pathway and, through this pathway, increases sarcolemmal NHE (Na + /H + exchanger) activity [Haworth, McCann, Snabaitis...

... myocytes. Although transduction of adenovirus harbouring H11 at low doses increased the cell size, at higher doses it induced apoptosis in cardiac myocytes. Apoptosis was not observed when adenovirus harbouring H11-KI (kinase-inactive mutant of H11) was used, suggesting that the proapoptotic effect of H11...

... rat cardiac myocytes). We therefore determined if the in vitro phosphorylation of substrates in these fractions could be used as assays of PKCα or ε activation. Intact cell phorbol ester treatment caused a decline in the in vitro 32 P-incorporation into several proteins in the cell-soluble fraction...