...Tania Martins-Marques; Steve Catarino; Monica Zuzarte; Carla Marques; Paulo Matafome; Paulo Pereira; Henrique Girão In this study we demonstrate that ischemia-induced impairment of intercellular communication between cardiomyocytes is due to the degradation of the gap junction protein Connexin43...

... contractions of rat cardiomyocytes and slowing their twitch relaxation; however, it did not induce spontaneous twitches. AdE-1 increased the duration of the cardiomyocyte action potential and decreased its amplitude and its time-to-peak in a concentration-dependent manner, without affecting its threshold...

... cysteine residue arrangement as sea anemone type 1 and 2 Na + channel toxins, its sequence contains many substitutions in conserved and essential sites and its overall homology to other toxins identified to date is low (<36%). Physiologically, AdE-1 increases the amplitude of cardiomyocyte contraction...

...Emre Amirak; Stephen J. Fuller; Peter H. Sugden; Angela Clerk ERK1/2 (extracellular-signal-regulated kinase 1/2) and their substrates RSKs (p90 ribosomal S6 kinases) phosphorylate different transcription factors, contributing differentially to transcriptomic profiles. In cardiomyocytes ERK1/2...

... penicillin and 100 μg/ml streptomycin, and transiently transfected with VDAC2 and RB#4 plasmid DNA at 70% confluence using Lipofectamine™ LTX (Invitrogen) according to the manufacturer's protocol. Adult rat cardiomyocytes were isolated from 10–14-week-old male Sprague–Dawley rats (240–260 g) after...

... robust and scalable, and validated commercial reagents become available. Production of human cardiomyocytes is now feasible on a daily basis for many laboratories with tissue culture expertise. An additional recent surge of interest resulting from the first production of human iPSCs (induced pluripotent...

...Scott P. Lawrence; Geoffrey D. Holman; Françoise Koumanov The Na + /H + exchanger NHE1 is a highly regulated membrane protein that is required for pH homoeostasis in cardiomyocytes. The activation of NHE1 leads to proton extrusion, which is essential for counteracting cellular acidity that occurs...

... or ischaemia [ 1 , 2 ]. However, this concept has proved technically difficult to examine using direct indices of mitochondrial function in intact myocytes. cardiomyocyte extracellular flux glycolysis heart lipid peroxidation mitochondrion oxidative stress Secondary products of oxidative stress...

...Katrin Bühler; Isabelle Plaisance; Thomas Dieterle; Marijke Brink Ucn2 (urocortin 2) has been shown to exert potent beneficial effects in the cardiovascular system, including inhibition of apoptosis, improvement of cardiomyocyte contractility and decrease of oxidative stress. The mechanisms...

... in the regulation of processes unrelated to stress, for example, in T lymphocytes and cardiomyocytes. In order to identify molecular targets responsible for the housekeeping functions of p38 MAPKs, we have analysed the differences in the transcriptomes of normally proliferating wild-type and p38α knockout...

...Katrina A. BICKNELL; Carmen H. COXON; Gavin BROOKS Repair of the mature mammalian myocardium following injury is impaired by the inability of the majority of cardiomyocytes to undergo cell division. We show that overexpression of the cyclin B1–CDC2 (cell division cycle 2 kinase) complex re...

... cardiomyocytes. The 2307bp 5′-flanking region of the rat ANP gene was cloned and fused to the luciferase gene. Evidence of the promoter activity was only apparent in the myocytes and was induced by hypoxia and HIF-1 inducers. The overexpression of HIF-1α markedly enhanced ANP promoter activity, and a dominant...