... ]. The tentative sequence was used as the basis for a BLASTP search of Rba. sphaeroides , identifying the N-terminal transmembrane helix of a 102 amino acid protein with a molecular mass of 10 564 Da as the best candidate; we provisionally name this component as protein-Z. bacteriochlorophylls carotenoids...

..., which is stabilized by a series of protein–protein, BChl–protein, BChl–BChl, carotenoid–BChl and carotenoid–protein contacts. LH1α consists of a short N-terminal helix of nine residues that lies parallel to the surface of the membrane on the cytoplasmic side, followed by a transmembrane domain and a 14...

... to be exclusive to the core ChlG–HliD assembly, is unclear. Here we generated mutants of Synechocystis sp. PCC 6803 lacking specific combinations of carotenoids or HliD in a background with FLAG- or His-tagged ChlG. Immunoprecipitation experiments and analysis of isolated membranes demonstrate that the absence...