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Keywords: caspase
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Articles

Evaluation of the effects of phosphorylation of synthetic peptide substrates on their cleavage by caspase-3 and -7

Izabela Maluch, Justyna Grzymska, Scott J. Snipas, Guy S. Salvesen, Marcin Drag
Journal: Biochemical Journal
Biochem J (2021) 478 (12): 2233–2245.
https://doi.org/10.1042/BCJ20210255
Published: 18 June 2021
...Izabela Maluch; Justyna Grzymska; Scott J. Snipas; Guy S. Salvesen; Marcin Drag Caspases are a family of enzymes that play roles in cell death and inflammation. It has been suggested that in the execution phase of the apoptotic pathway, caspase-3, -6 and -7 are involved. The substrate specificities...
View articletitled, Evaluation of the effects of phosphorylation of synthetic peptide substrates on their cleavage by caspase-3 and -7
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Includes: Supplementary data
Articles

Resurrection of ancestral effector caspases identifies novel networks for evolution of substrate specificity

Robert D. Grinshpon, Suman Shrestha, James Titus-McQuillan, Paul T. Hamilton, Paul D. Swartz, A. Clay Clark
Journal: Biochemical Journal
Biochem J (2019) 476 (22): 3475–3492.
https://doi.org/10.1042/BCJ20190625
Published: 21 November 2019
...Robert D. Grinshpon; Suman Shrestha; James Titus-McQuillan; Paul T. Hamilton; Paul D. Swartz; A. Clay Clark Apoptotic caspases evolved with metazoans more than 950 million years ago (MYA), and a series of gene duplications resulted in two subfamilies consisting of initiator and effector caspases...
View articletitled, Resurrection of ancestral effector caspases identifies novel networks for evolution of substrate specificity
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Includes: Supplementary data
Articles

CrmA orthologs from diverse poxviruses potently inhibit caspases-1 and -8, yet cleavage site mutagenesis frequently produces caspase-1-specific variants

David T. Bloomer, Tanja Kitevska-Ilioski, Delara Pantaki-Eimany, Yanhao Ji, Mark A. Miles, Begona Heras, Christine J. Hawkins
Journal: Biochemical Journal
Biochem J (2019) 476 (9): 1335–1357.
https://doi.org/10.1042/BCJ20190202
Published: 07 May 2019
... the cleavage reaction, deforming the protease active site and preventing dissociation. Spi-2 proteins like CrmA potently inhibit caspases-1, -4 and -5, which produce proinflammatory cytokines, and caspase-8, which facilitates cytotoxic lymphocyte-mediated target cell death. It is not clear whether both...
View articletitled, CrmA orthologs from diverse poxviruses potently inhibit caspases-1 and -8, yet cleavage site mutagenesis frequently produces caspase-1-specific variants
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Includes: Supplementary data
Articles

Comparative structural analysis of the caspase family with other clan CD cysteine peptidases

Karen McLuskey, Jeremy C. Mottram
Journal: Biochemical Journal
Biochem J (2015) 466 (2): 219–232.
https://doi.org/10.1042/BJ20141324
Published: 20 February 2015
...Karen McLuskey; Jeremy C. Mottram Clan CD forms a structural group of cysteine peptidases, containing seven individual families and two subfamilies of structurally related enzymes. Historically, it is most notable for containing the mammalian caspases, on which the structures of the clan were...
View articletitled, Comparative structural analysis of the caspase family with other clan CD cysteine peptidases
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Includes: Supplementary data
Articles

The membrane adaptor LAT is proteolytically cleaved following Fas engagement in a tyrosine phosphorylation-dependent fashion

Antonio García-Blesa, Mikolaj Klossowicz, Carmen López-Osuna, Mario Martínez-Florensa, Bernard Malissen, Francisco J. García-Cózar, Arkadiusz Miazek, Enrique Aguado
Journal: Biochemical Journal
Biochem J (2013) 450 (3): 511–521.
https://doi.org/10.1042/BJ20121135
Published: 28 February 2013
... be addressed (email [email protected] ). 20 7 2012 6 12 2012 14 12 2012 14 12 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 caspase CD3 granzyme B membrane adaptors signalling T-cell receptor (TCR) T-lymphocytes detect foreign...
View articletitled, The membrane adaptor LAT is proteolytically cleaved following Fas engagement in a tyrosine phosphorylation-dependent fashion
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Articles

Inactivation of ceramide transfer protein during pro-apoptotic stress by Golgi disassembly and caspase cleavage

Suchismita Chandran, Carolyn E. Machamer
Journal: Biochemical Journal
Biochem J (2012) 442 (2): 391–401.
https://doi.org/10.1042/BJ20111461
Published: 13 February 2012
.... In the present study, we show that Golgi disassembly during pro-apoptotic stress induced by TNFα (tumour necrosis factor α) and anisomycin results in decreased levels of CERT at the Golgi region. This is accompanied by a caspase-dependent loss of full-length CERT and reduction in de novo SM synthesis. In vitro...
View articletitled, Inactivation of ceramide transfer protein during pro-apoptotic stress by Golgi disassembly and caspase cleavage
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Includes: Supplementary data
Articles

UHRF1 depletion causes a G 2 /M arrest, activation of DNA damage response and apoptosis

Amy L. Tien, Sucharita Senbanerjee, Atul Kulkarni, Raksha Mudbhary, Bernadette Goudreau, Shridar Ganesan, Kirsten C. Sadler, Chinweike Ukomadu
Journal: Biochemical Journal
Biochem J (2011) 435 (1): 175–185.
https://doi.org/10.1042/BJ20100840
Published: 15 March 2011
... regulation. In the present study, we show that depleting cancer cells of UHRF1 causes activation of the DNA damage response pathway, cell cycle arrest in G 2 /M-phase and apoptosis dependent on caspase 8. The DNA damage response in cells depleted of UHRF1 is illustrated by: phosphorylation of histone H2AX...
View articletitled, UHRF1 depletion causes a G 2 /M arrest, activation of DNA damage response and apoptosis
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Includes: Supplementary data
Articles

Identification of the BCL2/adenovirus E1B-19K protein-interacting protein 2 (BNIP-2) as a granzyme B target during human natural killer cell-mediated killing

Gina B. Scott, Paul A. Bowles, Erica B. Wilson, Josephine L. Meade, Boon Chuan Low, Adam Davison, G. Eric Blair, Graham P. Cook
Journal: Biochemical Journal
Biochem J (2010) 431 (3): 423–431.
https://doi.org/10.1042/BJ20091073
Published: 11 October 2010
... apoptosis via the cleavage of a repertoire of cellular proteins, leading to caspase activation and mitochondrial depolarization. A simple bioinformatics strategy identified a candidate granzyme B cleavage site in the widely expressed BNIP-2 (BCL2/adenovirus E1B-19K protein-interacting protein 2). Granzyme B...
View articletitled, Identification of the BCL2/adenovirus E1B-19K protein-interacting protein 2 (BNIP-2) as a granzyme B target during human natural killer cell-mediated killing
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Articles

Caspase cleavage of Ets-1 p51 generates fragments with transcriptional dominant-negative function

Souhaila Choul-Li, Catherine Leroy, Gabriel Leprivier, Clélia Laitem, David Tulasne, Marc Aumercier
Journal: Biochemical Journal
Biochem J (2010) 426 (2): 229–241.
https://doi.org/10.1042/BJ20090877
Published: 09 February 2010
... study, we have demonstrated that Ets-1 p51, but not the spliced variant Ets-1 p42, is processed in a caspase-dependent manner in Jurkat T-leukaemia cells undergoing apoptosis, resulting in three C-terminal fragments Cp20, Cp17 and Cp14 and a N-terminal fragment, Np36. In vitro cleavage of Ets-1 p51...
View articletitled, Caspase cleavage of Ets-1 p51 generates fragments with transcriptional dominant-negative function
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Articles

Cytoprotective effects of IAPs revealed by a small molecule antagonist

Stefanie Galbán, Clara Hwang, Julie M. Rumble, Karolyn A. Oetjen, Casey W. Wright, Alain Boudreault, Jon Durkin, John W. Gillard, James B. Jaquith, Stephen J. Morris, Colin S. Duckett
Journal: Biochemical Journal
Biochem J (2009) 417 (3): 765–771.
https://doi.org/10.1042/BJ20081677
Published: 16 January 2009
... of neoplastic cells, and synthetic IAP antagonists represent a promising novel class of chemotherapeutic agents. Early work focused on the ability of these compounds to block the caspase-inhibitory function of XIAP (X-linked IAP). However, recent studies have shown that IAP antagonists, although primarily...
View articletitled, Cytoprotective effects of IAPs revealed by a small molecule antagonist
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Articles

Mechanisms of neuronal death in disease: defining the models and the players

Elena M. Ribe, Esther Serrano-Saiz, Nsikan Akpan, Carol M. Troy
Journal: Biochemical Journal
Biochem J (2008) 415 (2): 165–182.
https://doi.org/10.1042/BJ20081118
Published: 25 September 2008
...Elena M. Ribe; Esther Serrano-Saiz; Nsikan Akpan; Carol M. Troy Dysregulation of life and death at the cellular level leads to a variety of diseases. In the nervous system, aberrant neuronal death is an outstanding feature of neurodegenerative diseases. Since the discovery of the caspase family...
View articletitled, Mechanisms of neuronal death in disease: defining the models and the players
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Articles

Stimuli-dependent cleavage of Dicer during apoptosis

Alexey A. Matskevich, Karin Moelling
Journal: Biochemical Journal
Biochem J (2008) 412 (3): 527–534.
https://doi.org/10.1042/BJ20071461
Published: 28 May 2008
... necrosis factor α). We have also investigated the integrity of Dicer, and provide evidence that Dicer is a target for caspases during apoptosis. The cleavage of Dicer is stimulidependent and more pronounced when apoptosis is induced by PKC (protein kinase C) inhibitors, and can also be observed in HIV-1...
View articletitled, Stimuli-dependent cleavage of Dicer during apoptosis
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Articles

Requirement of Apaf-1 for mitochondrial events and the cleavage or activation of all procaspases during genotoxic stress-induced apoptosis

Emily E. Franklin, John D. Robertson
Journal: Biochemical Journal
Biochem J (2007) 405 (1): 115–122.
https://doi.org/10.1042/BJ20061576
Published: 13 June 2007
...Emily E. Franklin; John D. Robertson Sequential activation of caspases is critical for the execution of apoptosis. Recent evidence suggests caspase 2 is a significant upstream caspase capable of initiating mitochondrial events, such as the release of cytochrome c . In particular, in vitro studies...
View articletitled, Requirement of Apaf-1 for mitochondrial events and the cleavage or activation of all procaspases during genotoxic stress-induced apoptosis
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Articles

Comparing calpain- and caspase-3-mediated degradation patterns in traumatic brain injury by differential proteome analysis

Ming Cheng Liu, Veronica Akle, Wenrong Zheng, Jitendra R. Dave, Frank C. Tortella, Ronald L. Hayes, Kevin K. W. Wang
Journal: Biochemical Journal
Biochem J (2006) 394 (3): 715–725.
https://doi.org/10.1042/BJ20050905
Published: 24 February 2006
...Ming Cheng Liu; Veronica Akle; Wenrong Zheng; Jitendra R. Dave; Frank C. Tortella; Ronald L. Hayes; Kevin K. W. Wang A major theme of TBI (traumatic brain injury) pathology is the over-activation of multiple proteases. We have previously shown that calpain-1 and -2, and caspase-3 simultaneously...
View articletitled, Comparing calpain- and caspase-3-mediated degradation patterns in traumatic brain injury by differential proteome analysis
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Includes: Supplementary data
Articles

Suppression of the pro-apoptotic function of cytochrome c by singlet oxygen via a haem redox state-independent mechanism

Daisuke Suto, Kazuaki Sato, Yoshihiro Ohba, Tetsuhiko Yoshimura, Junichi Fujii
Journal: Biochemical Journal
Biochem J (2005) 392 (2): 399–406.
https://doi.org/10.1042/BJ20050580
Published: 22 November 2005
...), caspase-9 and other cofactors. The issue of whether the redox state of the haem in cyt c affects the initiation of the apoptotic pathway is currently a subject of debate. In a cell-free reconstitution system, we found that only oxidized cyt c was capable of activating the caspase cascade. Oxidized cyt c...
View articletitled, Suppression of the pro-apoptotic function of cytochrome c by singlet oxygen via a haem redox state-independent mechanism
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Includes: Supplementary data
Articles

Mitochondrial reactive oxygen species regulate the temporal activation of nuclear factor κB to modulate tumour necrosis factor-induced apoptosis: evidence from mitochondria-targeted antioxidants

Gillian HUGHES, Michael P. MURPHY, Elizabeth C. LEDGERWOOD
Journal: Biochemical Journal
Biochem J (2005) 389 (1): 83–89.
https://doi.org/10.1042/BJ20050078
Published: 21 June 2005
... in the mitochondrial matrix) enhanced TNF-induced apoptosis of U937 cells. In time course analyses, cleavage and activation of caspase 8 in response to TNF were not affected by MitoVit E, whereas the activation of caspase 3 was significantly increased. Furthermore, there was an increased cleavage of the proapoptotic...
View articletitled, Mitochondrial reactive oxygen species regulate the temporal activation of nuclear factor κB to modulate tumour necrosis factor-induced apoptosis: evidence from mitochondria-targeted antioxidants
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Articles

An abortive apoptotic pathway induced by singlet oxygen is due to the suppression of caspase activation

Kaoru OTSU, Kazuaki SATO, Yoshitaka IKEDA, Hirotaka IMAI, Yasuhito NAKAGAWA, Yoshihiro OHBA, Junichi FUJII
Journal: Biochemical Journal
Biochem J (2005) 389 (1): 197–206.
https://doi.org/10.1042/BJ20042067
Published: 21 June 2005
.... MNPE treatment induced much less DNA ladder formation and nuclear fragmentation in cells than etoposide treatment, even though these treatments induced a similar extent of cellular damage. Singlet oxygen inhibited caspase 9 and 3 activities directly and also suppressed the activation of the caspase...
View articletitled, An abortive apoptotic pathway induced by singlet oxygen is due to the suppression of caspase activation
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Articles

The BIR domain of IAP-like protein 2 is conformationally unstable: implications for caspase inhibition

Hwain SHIN, Martin RENATUS, Brendan P. ECKELMAN, Viviane A. NUNES, Claudio A. M. SAMPAIO, Guy S. SALVESEN
Journal: Biochemical Journal
Biochem J (2005) 385 (1): 1–10.
https://doi.org/10.1042/BJ20041107
Published: 14 December 2004
...Hwain SHIN; Martin RENATUS; Brendan P. ECKELMAN; Viviane A. NUNES; Claudio A. M. SAMPAIO; Guy S. SALVESEN Several IAP (inhibitor of apoptosis) proteins regulate cell fate decisions, and the X-linked IAP (XIAP) does so in part by inhibiting caspases, proteases that execute the apoptotic pathway...
View articletitled, The BIR domain of IAP-like protein 2 is conformationally unstable: implications for caspase inhibition
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Articles

IAP proteins: sticking it to Smac

Colin S. DUCKETT
Journal: Biochemical Journal
Biochem J (2005) 385 (1): E1.
https://doi.org/10.1042/BJ20041800
Published: 14 December 2004
...Colin S. DUCKETT Dogma has it that suppression of the programmed cell death pathway by the IAP (inhibitor of apoptosis) proteins is achieved by their direct enzymic inhibition of the chief executioners of the apoptotic process, the caspases. In turn, the IAPs themselves can be neutralized by Smac...
View articletitled, IAP proteins: sticking it to Smac
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Articles

Ionic interactions near the loop L4 are important for maintaining the active-site environment and the dimer stability of (pro)caspase 3

Brett FEENEY, Cristina POP, Ashutosh TRIPATHY, A. Clay CLARK
Journal: Biochemical Journal
Biochem J (2004) 384 (3): 515–525.
https://doi.org/10.1042/BJ20040693
Published: 07 December 2004
...Brett FEENEY; Cristina POP; Ashutosh TRIPATHY; A. Clay CLARK We have examined the role of a salt bridge between Lys 242 and Glu 246 in loop L4 of procaspase 3 and of mature caspase 3, and we show that the interactions are required for stabilizing the active site. Replacing either of the residues...
View articletitled, Ionic interactions near the loop L4 are important for maintaining the active-site environment and the dimer stability of (pro)caspase 3
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Articles

The protein structures that shape caspase activity, specificity, activation and inhibition

Pablo FUENTES-PRIOR, Guy S. SALVESEN
Journal: Biochemical Journal
Biochem J (2004) 384 (2): 201–232.
https://doi.org/10.1042/BJ20041142
Published: 23 November 2004
.... Fundamentally new mechanisms in protease regulation have been disclosed. Structural evidence suggests that caspases have an unusual catalytic mechanism, and that they are activated by apparently unrelated events, depending on which position in the apoptotic pathway they occupy. Some naturally occurring caspase...
View articletitled, The protein structures that shape caspase activity, specificity, activation and inhibition
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Includes: Supplementary data
Articles

The SARS coronavirus nucleocapsid protein induces actin reorganization and apoptosis in COS-1 cells in the absence of growth factors

In Collection
Coronavirus
Milan SURJIT, Boping LIU, Shahid JAMEEL, Vincent T. K. CHOW, Sunil K. LAL
Journal: Biochemical Journal
Biochem J (2004) 383 (1): 13–18.
https://doi.org/10.1042/BJ20040984
Published: 24 September 2004
..., and activated caspases 3 and 7. However, apoptosis was independent of the p53 and Fas signalling pathways. Furthermore, activation of the p38 MAPK pathway was found to induce actin reorganization in cells devoid of growth factors. At the cytoskeletal level, SARS-CoV N down-regulated FAK (focal adhesion kinase...
View articletitled, The SARS coronavirus nucleocapsid protein induces actin reorganization and apoptosis in COS-1 cells in the absence of growth factors
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Articles

Cell detachment and apoptosis induction of immortalized human prostate epithelial cells are associated with early accumulation of a 45 kDa nuclear isoform of clusterin

Alessandro E. CACCAMO, Maurizio SCALTRITI, Andrea CAPORALI, Domenico D'ARCA, Francesca SCORCIONI, Serenella ASTANCOLLE, Massimo MANGIOLA, Saverio BETTUZZI
Journal: Biochemical Journal
Biochem J (2004) 382 (1): 157–168.
https://doi.org/10.1042/BJ20040158
Published: 10 August 2004
... correspondence should be addressed (email [email protected] ). 28 1 2004 15 4 2004 12 5 2004 12 5 2004 The Biochemical Society, London 2004 anoikis apoptosis caspase clusterin PNT1A cell prostate cancer Clusterin is a glycoprotein widely distributed...
View articletitled, Cell detachment and apoptosis induction of immortalized human prostate epithelial cells are associated with early accumulation of a 45 kDa nuclear isoform of clusterin
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Articles

Signalling of the M 3 -muscarinic receptor to the anti-apoptotic pathway

David C. BUDD, Elizabeth J. SPRAGG, Katie RIDD, Andrew B. TOBIN
Journal: Biochemical Journal
Biochem J (2004) 381 (1): 43–49.
https://doi.org/10.1042/BJ20031705
Published: 22 June 2004
.... apoptosis Bcl-2 caspase G-protein-coupled receptor (GPCR) M 3 -muscarinic receptor transcription GPCRs (G-protein-coupled receptors) represent one of the largest gene families in the mammalian genome responding to ligands as diverse as biogenic amines, peptides, ions and light. The key role...
View articletitled, Signalling of the M 3 -muscarinic receptor to the anti-apoptotic pathway
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Articles

The regulatory domain of the inositol 1,4,5-trisphosphate receptor is necessary to keep the channel domain closed: possible physiological significance of specific cleavage by caspase 3

Tomohiro NAKAYAMA, Mitsuharu HATTORI, Keiko UCHIDA, Takeshi NAKAMURA, Yoko TATEISHI, Hiroko BANNAI, Miwako IWAI, Takayuki MICHIKAWA, Takafumi INOUE, Katsuhiko MIKOSHIBA
Journal: Biochemical Journal
Biochem J (2004) 377 (2): 299–307.
https://doi.org/10.1042/bj20030599
Published: 15 January 2004
... in generating complex Ca 2+ signalling patterns. IP 3 R1 consists of three domains: a ligand-binding domain, a regulatory domain and a channel domain. In order to investigate the function of these domains in its gating machinery and the physiological significance of specific cleavage by caspase 3...
View articletitled, The regulatory domain of the inositol 1,4,5-trisphosphate receptor is necessary to keep the channel domain closed: possible physiological significance of specific cleavage by caspase 3
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Articles

Caspase processing activates atypical protein kinase C ζ by relieving autoinhibition and destabilizes the protein

Lucinda SMITH, Zhi WANG, Jeffrey B. SMITH
Journal: Biochemical Journal
Biochem J (2003) 375 (3): 663–671.
https://doi.org/10.1042/bj20030926
Published: 01 November 2003
...Lucinda SMITH; Zhi WANG; Jeffrey B. SMITH Treatment of HeLa cells with tumour necrosis factor α (TNFα) induced caspase processing of ectopic PKC (protein kinase C) ζ, which converted most of the holoenzyme into the freed kinase domain and increased immune-complex kinase activity. The goal...
View articletitled, Caspase processing activates atypical protein kinase C ζ by relieving autoinhibition and destabilizes the protein
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Articles

Identification of ubiquitination sites on the X-linked inhibitor of apoptosis protein

Hwain SHIN, Kazuya OKADA, John C. WILKINSON, Katherine M. SOLOMON, Colin S. DUCKETT, John C. REED, Guy S. SALVESEN
Journal: Biochemical Journal
Biochem J (2003) 373 (3): 965–971.
https://doi.org/10.1042/bj20030583
Published: 01 August 2003
.... Key words: caspase, cell death, inhibitor of apoptosis protein (IAP), mass spectrometry, ubiquitin. INTRODUCTION Inhibitor of apoptosis proteins (IAPs), originally found in baculoviruses, are present in organisms from viruses to yeast to humans [1]. Characterized by the presence of one to three tandem...
View articletitled, Identification of ubiquitination sites on the X-linked inhibitor of apoptosis protein
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Articles

Molecular cloning and characterization of CIDE-3, a novel member of the cell-death-inducing DNA-fragmentation-factor (DFF45)-like effector family

Liang LIANG, Mujun ZHAO, Zhenhua XU, Kazunari K. YOKOYAMA, Tsaiping LI
Journal: Biochemical Journal
Biochem J (2003) 370 (1): 195–203.
https://doi.org/10.1042/bj20020656
Published: 15 February 2003
...Liang LIANG; Mujun ZHAO; Zhenhua XU; Kazunari K. YOKOYAMA; Tsaiping LI DNA fragmentation is one of the critical steps in apoptosis, which is induced by DNA fragmentation factor (DFF). DFF is composed of two subunits, a 40kDa caspase-activated nuclease (DFF40) and a 45kDa inhibitor (DFF45). Recently...
View articletitled, Molecular cloning and characterization of CIDE-3, a novel member of the cell-death-inducing DNA-fragmentation-factor (DFF45)-like effector family
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Articles

Oxidation-triggered c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase pathways for apoptosis in human leukaemic cells stimulated by epigallocatechin-3-gallate (EGCG): a distinct pathway from those of chemically induced and receptor-mediated apoptosis

Koichi SAEKI, Norihiko KOBAYASHI, Yuko INAZAWA, Hong ZHANG, Hideki NISHITOH, Hidenori ICHIJO, Kumiko SAEKI, Mamoru ISEMURA, Akira YUO
Journal: Biochemical Journal
Biochem J (2002) 368 (3): 705–720.
https://doi.org/10.1042/bj20020101
Published: 15 December 2002
... cells. In U937 cells, the apoptosis and activation of caspases-3 and −9 induced by EGCG but not VP16 and TNF were inhibited with SB203580, a specific inhibitor of p38, while those induced by EGCG and VP16 but not TNF were inhibited with SB202190, a rather broad inhibitor of JNK and p38. In contrast...
View articletitled, Oxidation-triggered c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase pathways for apoptosis in human leukaemic cells stimulated by epigallocatechin-3-gallate (EGCG): a distinct pathway from those of chemically induced and receptor-mediated apoptosis
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Articles

Positive feedback of protein kinase C proteolytic activation during apoptosis

Sabrina LEVERRIER, Alice VALLENTIN, Dominique JOUBERT
Journal: Biochemical Journal
Biochem J (2002) 368 (3): 905–913.
https://doi.org/10.1042/bj20021253
Published: 15 December 2002
...Sabrina LEVERRIER; Alice VALLENTIN; Dominique JOUBERT In contrast with protein kinase Cα (PKCα) and PKC∊, which are better known for promoting cell survival, PKCΔ is known for its pro-apoptotic function, which is exerted mainly through a caspase-3-dependent proteolytic activation pathway...
View articletitled, Positive feedback of protein kinase C proteolytic activation during apoptosis
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Articles

Induction of apoptosis in human leukaemic cells by IPENSpm, a novel polyamine analogue and anti-metabolite

Alison V. FRASER, Patrick M. WOSTER, Heather M. WALLACE
Journal: Biochemical Journal
Biochem J (2002) 367 (1): 307–312.
https://doi.org/10.1042/bj20020156
Published: 01 October 2002
.... IPENSpm was cytotoxic to these cells at concentrations greater than 2.5 μ M. It induced apoptosis in a caspase-dependent manner and its toxicity profile was comparable with etoposide, a well-known anti-tumour agent and inducer of apoptosis. IPENSpm decreased intracellular polyamine content as a result...
View articletitled, Induction of apoptosis in human leukaemic cells by IPENSpm, a novel polyamine analogue and anti-metabolite
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Articles

Doxorubicin and octreotide induce a 40 kDa breakdown product of p53 in human hepatoma and tumoral colon cell lines

Zahia SADJI-OUATAS, Malika LASFER, Sophie JULIEN, Gérard FELDMANN, Florence REYL-DESMARS
Journal: Biochemical Journal
Biochem J (2002) 364 (3): 881–885.
https://doi.org/10.1042/bj20020349
Published: 15 June 2002
... a cleavage of the p53 C-terminal region (p40ΔC). Furthermore, this cleavage was not dependent on caspase activity. In conclusion, these results support the hypothesis that this post-translational modification plays a significant role in the regulation of multiple p53 signalling pathways. These results also...
View articletitled, Doxorubicin and octreotide induce a 40 kDa breakdown product of p53 in human hepatoma and tumoral colon cell lines
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Articles

Activation of c-Jun N-terminal kinase promotes survival of cardiac myocytes after oxidative stress

Christopher J. DOUGHERTY, Lori A. KUBASIAK, Howard PRENTICE, Peter ANDREKA, Nanette H. BISHOPRIC, Keith A. WEBSTER
Journal: Biochemical Journal
Biochem J (2002) 362 (3): 561–571.
https://doi.org/10.1042/bj3620561
Published: 08 March 2002
... is protective. Transfection or infection with JNK inhibitory mutants increased the rates of apoptosis by almost 2-fold compared with control cultures grown aerobically or subjected to hypoxia and reoxygenation. Caspase 9 activity, measured by LEHD cleavage, increased > 3-fold during reoxygenation...
View articletitled, Activation of c-Jun N-terminal kinase promotes survival of cardiac myocytes after oxidative stress
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Articles

Measurement of proteases using chemiluminescence-resonance-energy-transfer chimaeras between green fluorescent protein and aequorin

Jonathan P. WAUD, Alexandra BERMÚDEZ FAJARDO, Thankiah SUDHAHARAN, Andrew R. TRIMBY, Jinny JEFFERY, Ann JONES, Anthony K. CAMPBELL
Journal: Biochemical Journal
Biochem J (2001) 357 (3): 687–697.
https://doi.org/10.1042/bj3570687
Published: 25 July 2001
... measurements in cell extracts or clinical samples. Here we demonstrate the principle of chemiluminescence resonance energy transfer (CRET) as a homogeneous assay system, using two proteases as models, one extracellular (α-thrombin) and the other intracellular (caspase-3). Chimaeras were engineered...
View articletitled, Measurement of proteases using chemiluminescence-resonance-energy-transfer chimaeras between green fluorescent protein and aequorin
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Articles

Rat embryo fibroblasts require both the cell-binding and the heparin-binding domains of fibronectin for survival

Jinsook JEONG, Innoc HAN, Yangmi LIM, Jungyean KIM, Ilseon PARK, Anne WOODS, John R. COUCHMAN, Eok-Soo OH
Journal: Biochemical Journal
Biochem J (2001) 356 (2): 531–537.
https://doi.org/10.1042/bj3560531
Published: 24 May 2001
... in the HepII domain [peptide V, WQPPRARI (single-letter amino acid codes)], which has previously been implicated in cytoskeletal organization, rescued apoptotic changes. Consistently, pp125FAK phosphorylation was increased, and both cleavage of pp125FAK and activation of caspase 3 on FN120 were partly blocked...
View articletitled, Rat embryo fibroblasts require both the cell-binding and the heparin-binding domains of fibronectin for survival
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Articles

Resistance of bone marrow-derived macrophages to apoptosis is associated with the expression of X-linked inhibitor of apoptosis protein in primary cultures of bone marrow cells

Hong LIN, Catheryne CHEN, Ben D.-M. CHEN
Journal: Biochemical Journal
Biochem J (2001) 353 (2): 299–306.
https://doi.org/10.1042/bj3530299
Published: 08 January 2001
... cells were induced to undergo proliferation and differentiation into mature macrophages in vitro with cell morphology similar to that of tissue macrophages by day 7Ő10. Immunoblot analyses showed that bone marrow precursors express appreciable levels of caspase-3 and caspase-9 but no or very low levels...
View articletitled, Resistance of bone marrow-derived macrophages to apoptosis is associated with the expression of X-linked inhibitor of apoptosis protein in primary cultures of bone marrow cells
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Articles

Apoptosis of haematopoietic cells upon thymidylate synthase inhibition is independent of p53 accumulation and CD95–CD95 ligand interaction

Cristina MUÑOZ-PINEDO, F. Javier OLIVER, Abelardo LÓPEZ-RIVAS
Journal: Biochemical Journal
Biochem J (2001) 353 (1): 101–108.
https://doi.org/10.1042/bj3530101
Published: 18 December 2000
... decreased following FUdR treatment. Moreover, blocking CD95–CD95L interactions with antagonistic CD95 monoclonal antibody did not prevent drug-induced apoptosis. Furthermore, analysis of caspase involvement showed important differences in apoptosis induced by CD95-triggering or FUdR treatment. In summary...
View articletitled, Apoptosis of haematopoietic cells upon thymidylate synthase inhibition is independent of p53 accumulation and CD95–CD95 ligand interaction
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Articles

Polyamines directly induce release of cytochrome c from heart mitochondria

Claudio STEFANELLI, Ivana STANIC', Maddalena ZINI, Francesca BONAVITA, Flavio FLAMIGNI, Laura ZAMBONIN, Laura LANDI, Carla PIGNATTI, Carlo GUARNIERI, Claudio M. CALDARERA
Journal: Biochemical Journal
Biochem J (2000) 347 (3): 875–880.
https://doi.org/10.1042/bj3470875
Published: 25 April 2000
... of the caspase cascade. In this report, we show that addition of micromolar concentrations of polyamines to isolated rat heart mitochondria induces the release of cytochrome c . Spermine, which is effective at concentrations of 10-100 μ M, is more potent than spermidine, whereas putrescine has no effect up to 1...
View articletitled, Polyamines directly induce release of cytochrome c from heart mitochondria
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Articles

Heat-shock protein 70 antisense oligomers enhance proteasome inhibitor-induced apoptosis

John D. ROBERTSON, Kaushik DATTA, Shyam S. BISWAL, James P. KEHRER
Journal: Biochemical Journal
Biochem J (1999) 344 (2): 477–485.
https://doi.org/10.1042/bj3440477
Published: 24 November 1999
... that proteasome activities were the same in these two cell lines. At 24 h after treatment with 500 nM MG132, apoptosis in bcl-x L cells (22%) was less than that observed in control cells (34%). Concomitantly, caspase activity in control cells, as assessed by N -acetyl- L -aspartyl- L -glutamyl- L -valyl- L...
View articletitled, Heat-shock protein 70 antisense oligomers enhance proteasome inhibitor-induced apoptosis
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