... proteins. A role of IQGAP1 in adipocytes has not been reported. We therefore investigated the cellular IQGAP1 interactome in primary human adipocytes. Immunoprecipitation and quantitative mass spectrometry identified caveolae and caveolae-associated proteins as the major IQGAP1 interactors alongside...

.... The glycolytic enzyme ENO-1 (enolase-1) is translocated from the cytoplasm to the cell surface, where it binds PLG (plasminogen) to enhance pericellular plasmin production and cell motility. In the present study, ENO-1 was found to localize to a specialized subset of lipid rafts called caveolae as demonstrated...

...Richard R. Sprenger; Ruud D. Fontijn; Jan van Marle; Hans Pannekoek; Anton J. G. Horrevoets Lipid rafts and caveolae are biochemically similar, specialized domains of the PM (plasma membrane) that cluster specific proteins. However, they are morphologically distinct, implying different, possibly...

...Nabila ABOULAICH; Julia P. VAINONEN; Peter STRÅLFORS; Alexander V. VENER Caveolae, the specialized invaginations of plasma membranes, formed sealed vesicles with outwards-orientated cytosolic surface after isolation from primary human adipocytes. This morphology allowed differential, vectorial...

... domain of phospholipase C-γ1; this interaction was not, however, detected under more physiological conditions. Specific anti-peptide antisera identified SR-BII in human monocyte/macrophage THP-1 cells and, in recombinant cells, revealed receptor localization to caveolae, a plasma membrane microdomain...

... the beads were internalized, and their subsequent intracellular routing, was strongly dependent on particle size. Internalization of microspheres with a diameter <200 nm involved clathrin-coated pits. With increasing size, a shift to a mechanism that relied on caveolae-mediated internalization became...

... To whom correspondence should be addressed (e-mail [email protected] ). 4 10 2001 20 10 2001 19 11 2001 The Biochemical Society, London ©2002 2002 caveolae hydrogen peroxide post-translational modification Abbreviation used: ECs, endothelial cells. Biochem. J. (2002...

... with and potentiated the transactivation of an ERa mutant that cannot be phosphorylated by extracellular signal-regulated kinase (ERK)1}2 [ERa(SerAla Thus caveolin-1 is a novel ERa regulator that drives ERK1}2- independent phosphorylation and activation of AF-1. Key words: caveolae, receptor activation, sex hormone...

... investigated the sphingomyelinase content of caveolae, invaginated plasma-membrane microdomains that contain a variety of signalling molecules. These structures are highly enriched in sphingomyelin as well as in ceramide, which suggests that metabolism of these lipids might, to some extent, occur locally...

... inhibited by K44A dynamin (22.4±3.7%), and was insensitive to arrestin-(319–418), which are dominant-negative mutants of dynamin I and β-arrestin respectively. Furthermore, the B 2 R-mediated sequestration of BK was completely insensitive to both mutants, as was the association of BK with a caveolae...

... acylated a-subunits Key words: fatty acylation, caveolae, palmitoylation, my- ristoylation. buted throughout the plasma membrane, but enriched within Triton-insoluble low-buoyancy domains rich in sphingomyelin, glycosphingolipids and cholesterol [hereafter termed rafts or Triton-insoluble floating...

... into the cell. Key words: caveolae, cholesterol, coated pits, endocytosis, lipoprotein. extracellular matrix [5], or a possible role for the syndecan transmembrane domain on the basis of the persistence of detergent insolubility after deletion of all except the first four intracellular amino acyl residues [6...

... characteristics of detergent-insoluble glycolipid-rich domains (DIGs). However, ultrastructural analysis of the CLM fraction revealed that it contained a heterogeneous collection of vesicles, some with sizes greater than that expected for individual caveolae. Experiments with detergent-solubilized cells...