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Keywords: ceramide
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Articles
Biochem J (2021) 478 (19): 3621–3642.
Published: 14 October 2021
...Botheina Ghandour; Ghassan Dbaibo; Nadine Darwiche Sphingolipid-mediated regulation in cancer development and treatment is largely ceramide-centered with the complex sphingolipid metabolic pathways unfolding as attractive targets for anticancer drug discovery. The dynamic interconversion...
Articles
Biochem J (2016) 473 (5): 593–603.
Published: 24 February 2016
...Kyle D. Luttgeharm; Edgar B. Cahoon; Jonathan E. Markham Ceramide makes up the acyl-backbone of sphingolipids and plays a central role in determining the function of these essential membrane lipids. In Arabidopsis, the varied chemical composition of ceramide is determined by the specificity...
Includes: Supplementary data
Articles
Biochem J (2015) 471 (1): 37–51.
Published: 21 September 2015
... ) increased phosphatase resistance of the phosphorylated activation loop (pThr 308 ) and amplified Akt phosphorylation. Furthermore, the phosphatase-resistant Akt was refractory to ceramide-dependent dephosphorylation and amplified insulin-dependent Thr 308 phosphorylation in a regulated fashion. Collectively...
Includes: Supplementary data
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Biochem J (2015) 465 (3): 371–382.
Published: 22 January 2015
... levels of ceramide. This manuscript shows that nSMase-2 is a redox-sensitive enzyme that oligomerizes via Cys 617 -mediated intermolecular bonds that is linked to suppressed enzyme activity, and may be a substrate for thioredoxin. ceramide cysteine oligomerization oxidative stress...
Includes: Supplementary data
Articles
Biochem J (2014) 458 (1): 81–93.
Published: 20 January 2014
... and initiates intracellular signalling cascades that promote malignant transformation, but the underlying mechanisms for c-Src translocation remain unclear. In the present study we show that c-Src up-regulation perturbs sphingolipid/cholesterol-enriched membrane microdomains by activating ceramide synthesis...
Includes: Supplementary data
Articles
Biochem J (2013) 456 (3): 427–439.
Published: 22 November 2013
...Melissa E. Smith; Trevor S. Tippetts; Eric S. Brassfield; Braden J. Tucker; Adelaide Ockey; Adam C. Swensen; Tamil S. Anthonymuthu; Trevor D. Washburn; Daniel A. Kane; John T. Prince; Benjamin T. Bikman Ceramide is a sphingolipid that serves as an important second messenger in an increasing number...
Includes: Supplementary data
Articles
Biochem J (2013) 452 (1): 111–119.
Published: 25 April 2013
... combinations of drugs that more potently kill cancer cells while sparing normal cells. The BCL2 family of proteins and bioactive sphingolipids are intricately linked during apoptotic cell death. In fact, many chemotherapeutic drugs are known to cause accumulation of the pro-apoptotic sphingolipid ceramide...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (1): 79–88.
Published: 26 April 2012
... ]. In fact, S1P-neutralizing monoclonal antibodies have been shown to reduce tumour progression in animal xenograft and allograft models [ 6 ]. ceramide sphingosine sphingosine kinase (SphK) sphingosine kinase inhibitor (SphK inhibitor) sphingosine 1-phosphate (S1P) Total RNA was isolated from...
Includes: Supplementary data
Articles
Biochem J (2012) 441 (3): 789–802.
Published: 16 January 2012
...Thomas D. Mullen; Yusuf A. Hannun; Lina M. Obeid Sphingolipid metabolism in metazoan cells consists of a complex interconnected web of numerous enzymes, metabolites and modes of regulation. At the centre of sphingolipid metabolism reside CerSs (ceramide synthases), a group of enzymes that catalyse...
Articles
Biochem J (2011) 438 (1): 177–189.
Published: 27 July 2011
...Julien Véret; Nicolas Coant; Evgeny V. Berdyshev; Anastasia Skobeleva; Nicole Therville; Danielle Bailbé; Irina Gorshkova; Viswanathan Natarajan; Bernard Portha; Hervé Le Stunff Pancreatic β-cell apoptosis induced by palmitate requires high glucose concentrations. Ceramides have been suggested...
Includes: Supplementary data
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Biochem J (2011) 438 (1): 165–175.
Published: 27 July 2011
...) analysis of sphingolipids based on both mass and hydrophobicity, and use this method to characterize the SM (sphingomyelin), ceramide and GalCer (galactosylceramide) content of hippocampus from AD (Alzheimer's disease) and control subjects. Using a mathematical relationship we exclude the influence...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (2): 381–390.
Published: 29 March 2011
... or untagged nSMase3 in multiple cell lines had no significant effect on in vitro N-SMase activity. Moreover, only overexpression of nSMase2, but not nSMase1 or nSMase3, had significant effects on cellular sphingolipid levels, increasing ceramide and decreasing sphingomyelin. Additionally, only siRNA (small...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (1): 267–276.
Published: 15 March 2011
... by 70% under these conditions, without any significant alteration in the amounts of either ceramide or sphingomyelin. However, flux into ceramide (measured by [ 3 H]serine incorporation) was augmented by chronic palmitate, and inhibition of ceramide synthesis decreased both ER stress and apoptosis. ER...
Includes: Supplementary data
Articles
Biochem J (2010) 427 (2): 265–274.
Published: 29 March 2010
...Jolanta Idkowiak-Baldys; Aintzane Apraiz; Li Li; Mehrdad Rahmaniyan; Christopher J. Clarke; Jacqueline M. Kraveka; Aintzane Asumendi; Yusuf A. Hannun Oxidative stress has been implicated previously in the regulation of ceramide metabolism. In the present study, its effects on dihydroceramide...
Articles
Biochem J (2009) 424 (2): 273–283.
Published: 11 November 2009
...Stefka D. Spassieva; Thomas D. Mullen; Danyelle M. Townsend; Lina M. Obeid Ceramide metabolism has come under recent scrutiny because of its role in cellular stress responses. CerS2 (ceramide synthase 2) is one of the six mammalian isoforms of ceramide synthase and is responsible for the synthesis...
Includes: Supplementary data
Articles
Biochem J (2009) 417 (3): 791–801.
Published: 16 January 2009
...Maria L. Watson; Matthew Coghlan; Harinder S. Hundal Saturated fatty acids, such as palmitate, promote accumulation of ceramide, which impairs activation and signalling of PKB (protein kinase B; also known as Akt) to important end points such as glucose transport. SPT (serine palmitoyl transferase...
Includes: Supplementary data
Articles
Biochem J (2008) 414 (1): 31–41.
Published: 29 July 2008
...Maristella Villani; Marimuthu Subathra; Yeong-Bin Im; Young Choi; Paola Signorelli; Maurizio Del Poeta; Chiara Luberto SMS [SM (sphingomyelin) synthase] is a class of enzymes that produces SM by transferring a phosphocholine moiety on to ceramide. PC (phosphatidylcholine) is believed...
Includes: Supplementary data
Articles
Biochem J (2008) 410 (2): 381–389.
Published: 12 February 2008
..., which enrich the intranuclear diacylglycerol pool, and the effect of phosphatidylcholine-specific phospholipase C activity on neutral sphingomyelinase and reverse sphingomyelin synthase, which enrich the intranuclear ceramide pool, was investigated. The results show that in chromatin, there exists...
Articles
Biochem J (2008) 410 (2): 369–379.
Published: 12 February 2008
...Eric Hajduch; Sophie Turban; Xavier Le Liepvre; Soazig Le Lay; Christopher Lipina; Nikolaos Dimopoulos; Isabelle Dugail; Harinder S. Hundal Elevated ceramide concentrations in adipocytes and skeletal muscle impair PKB (protein kinase B; also known as Akt)-directed insulin signalling to key hormonal...
Includes: Supplementary data
Articles
Biochem J (2008) 410 (1): 81–92.
Published: 29 January 2008
... in these disease states remains unknown. In the present study, we investigated the sequelae of NB (neuroblastoma) cells upon sulfatide supplementation and the biochemical mechanisms contributing to the sulfatide-induced changes. By using shotgun lipidomics, we showed dramatic accumulations of sulfatide, ceramide...
Articles
Biochem J (2007) 407 (3): 383–395.
Published: 12 October 2007
...Claudio Costantini; Mi Hee Ko; Mary Cabell Jonas; Luigi Puglielli The lipid second messenger ceramide regulates the rate of β cleavage of the Alzheimer's disease APP (amyloid precursor protein) by affecting the molecular stability of the β secretase BACE1 (β-site APP cleaving enzyme 1...
Includes: Supplementary data
Articles
Biochem J (2007) 405 (1): 157–164.
Published: 13 June 2007
.... Argraves K. M. Enzymes of sphingolipid metabolism: from modular to integrative signaling Biochemistry 2001 40 4893 4903 2 Hannun Y. A. Obeid L. M. The ceramide-centric universe of lipid-mediated cell regulation: stress encounters of the lipid kind J. Biol. Chem. 2002 277...
Articles
Biochem J (2007) 401 (1): 205–216.
Published: 11 December 2006
... for ceramide synthesis by the Lag1p (longevity-assurance gene 1)/Lac1p (longevity-assurance gene cognate 1)/Lip1p (Lag1p/Lac1p interacting protein) ceramide synthase. LAG1 and LAC1 are redundant but LIP1 is essential. Here we show that 4Δ ( lag1 Δ lac1 Δ ypc1 Δ ydc1 Δ) cells devoid of all known endogenous...
Includes: Supplementary data
Articles
Biochem J (2006) 395 (2): 311–318.
Published: 28 March 2006
.... This study was undertaken to gain insight into the molecular alterations producing PTX resistance in ovarian cancer. PTX treatment is able to induce apoptosis in the human ovarian carcinoma cell line, CABA I. PTX-induced apoptosis in CABA I cells was accompanied by an increase in the cellular Cer (ceramide...
Articles
Biochem J (2006) 393 (3): 733–740.
Published: 13 January 2006
...L. Ashley Cowart; Yasuo Okamoto; Xinghua Lu; Yusuf A. Hannun Saccharomyces cerevisiae produces the sphingolipid ceramide by de novo synthesis as well as by hydrolysis of complex sphingolipids by Isc1p (inositolphosphoceramide-phospholipase C), which is homologous with the mammalian neutral...
Includes: Supplementary data
Articles
Biochem J (2006) 393 (2): 513–521.
Published: 23 December 2005
... of endogenous long-chain ceramide in response to exogenous C 6 -cer (C 6 -ceramide), which is FB1 (fumonisin B1)-sensitive, was employed to probe the sphingosine-recycling pathway. The data showed that ceramide formation via this pathway was significantly blocked by GSH and NAC ( N -acetylcysteine) whereas...
Articles
Biochem J (2005) 392 (1): 231–239.
Published: 08 November 2005
...-negative malignant T-cells [ 8 ]. It was observed that HPR-mediated growth inhibition was associated with ceramide accumulation only in HTLV-I-negative cells. The aim of the present study was to investigate the mechanism by which HPR differentially regulates ceramide metabolism in HTLV-I-negative and HTLV...
Articles
Biochem J (2005) 391 (1): 59–67.
Published: 26 September 2005
... reduces, whereas p75 NTR activates, β-cleavage of APP. The p75 NTR -dependent effect requires NGF (nerve growth factor) binding and activation of the second messenger ceramide. We also show that normal aging activates Aβ generation in the brain by ‘switching’ from the TrkA to the p75 NTR receptor system...
Includes: Supplementary data
Articles
Biochem J (2005) 388 (1): 245–254.
Published: 10 May 2005
...Matthias ECKHARDT; Afshin YAGHOOTFAM; Simon N. FEWOU; Inge ZÖLLER; Volkmar GIESELMANN Hydroxylation is an abundant modification of the ceramides in brain, skin, intestinal tract and kidney. Hydroxylation occurs at the sphingosine base at C-4 or within the amide-linked fatty acid. In myelin...
Articles
Biochem J (2005) 388 (1): 169–176.
Published: 10 May 2005
...-Sanchez (2003) Biochem. J. 373 , 917–924]. In yeast cells, IPC (inositol phosphoceramide) synthase catalyses the transfer of phosphoinositol from phosphatidylinositol to ceramide to form IPC and generates DAG. In the present study, we found that, during the G 1 to S transition after N 2 -starvation...
Articles
Biochem J (2005) 386 (3): 445–451.
Published: 08 March 2005
...Helene BIRBES; Chiara LUBERTO; Yi-Te HSU; Samer EL BAWAB; Yusuf A. HANNUN; Lina M. OBEID We recently showed that targeting bSMase (bacterial sphingomyelinase) specifically to mitochondria caused accumulation of ceramide in mitochondria, and induced cytochrome c release and cell death [Birbes, El...
Articles
Biochem J (2005) 385 (3): 685–693.
Published: 24 January 2005
...Greg PLUMMER; Kathleen R. PERREAULT; Charles F. B. HOLMES; Elena I. POSSE de CHAVES In sympathetic neurons, C 6 -ceramide, as well as endogenous ceramides, blocks apoptosis elicited by NGF (nerve growth factor) deprivation. The mechanism(s) involved in ceramide-induced neuronal survival are poorly...
Articles
Biochem J (2004) 382 (2): 527–533.
Published: 24 August 2004
...Clara BIONDA; Jacques PORTOUKALIAN; Daniel SCHMITT; Claire RODRIGUEZ-LAFRASSE; Dominique ARDAIL Recent studies by our group and others have disclosed the presence of ceramides in mitochondria, and the activities of ceramide synthase and reverse ceramidase in mitochondria have also been reported...
Articles
Biochem J (2004) 380 (3): 715–722.
Published: 15 June 2004
...Kou-Yi TSERNG; Ronda L. GRIFFIN Ceramides, which are produced from the hydrolysis of sphingomyelin or synthesized from serine and palmitate in a de novo pathway, are regarded as important cellular signals for inducing apoptosis. However, controversy over this proposed role of ceramides exists...
Articles
Biochem J (2004) 380 (3): 907–918.
Published: 15 June 2004
...Nils J. FÆRGEMAN; Søren FEDDERSEN; Janne K. CHRISTIANSEN; Morten K. LARSEN; Roger SCHNEITER; Christian UNGERMANN; Kudzai MUTENDA; Peter ROEPSTORFF; Jens KNUDSEN In the present study, we show that depletion of acyl-CoA-binding protein, Acb1p, in yeast affects ceramide levels, protein trafficking...
Articles
Biochem J (2004) 380 (2): 435–440.
Published: 01 June 2004
...Arkaitz CARRACEDO; Math J. H. GEELEN; María DIEZ; Kentaro HANADA; Manuel GUZMÁN; Guillermo VELASCO Cannabinoids induce apoptosis on glioma cells via stimulation of ceramide synthesis de novo , whereas they do not affect viability of primary astrocytes. In the present study, we show that incubation...
Articles
Biochem J (2004) 378 (2): 335–342.
Published: 01 March 2004
.... Activation of PP1 by treatment of the cells with ceramide suppressed Ser-23 phosphorylation, as did transfection of the catalytic subunit of PP1. Phosphorylation at Ser-23 is also increased in a transient manner upon T-cell antigen receptor ligation. In contrast, treatment of cells with phorbol ester had...
Articles
Biochem J (2003) 376 (3): 697–705.
Published: 15 December 2003
... of PKB by insulin was inhibited by pretreatment of the hepatocytes with C2 ceramide. This resulted in the inhibition of insulin-dependent increases in GCK and SREBP1 mRNAs. A triple mutant of PKB failed to interfere with insulin activation of PKB in hepatocytes even at high overexpression levels achieved...
Articles
Biochem J (2003) 376 (3): 725–732.
Published: 15 December 2003
... of the sphingolipid ceramide and reactive oxygen species are among these pathways. Crossregulation of these two pathways is possible owing to the demonstrated inhibition of neutral sphingomyelinase by glutathione, the predominant cellular antioxidant, and has been observed in some cytokine-dependent cell-death models...
Articles
Biochem J (2003) 376 (2): 473–479.
Published: 01 December 2003
... ceramidase is available on the DNA Data Bank of Japan (DDBJ). 2 5 2003 12 8 2003 28 8 2003 28 8 2003 The Biochemical Society, London ©2003 2003 ceramidase ceramide Dictyostelium discoideum pH dependency sphingolipid Abbreviations used: CDase, ceramidase; Cer...
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Biochem J (2003) 375 (3): 567–579.
Published: 01 November 2003
...Darren C. PHILLIPS; Helen R. GRIFFITHS Ceramide (a sphingolipid) and reactive oxygen species are each partly responsible for intracellular signal transduction in response to a variety of agents. It has been reported that ceramide and reactive oxygen species are intimately linked and show reciprocal...
Articles
Biochem J (2003) 374 (2): 307–314.
Published: 01 September 2003
... moiety is coincident with increased compound-induced cytotoxicity. Therefore, in the present study, we examined the mechanism of this cytotoxicity in detail. Despite N -butyl-deoxynojirimycin and N -butyl-deoxygalactonojirimycin inhibiting the glycosylation of ceramide to glucosylceramide, ceramide...
Articles
Biochem J (2003) 371 (2): 621–629.
Published: 15 April 2003
... results in activation of caspase 3, implying induction of apoptosis. DHA (10 μ M) is shown to increase ceramide levels after 6h of incubation and, after 24h, the cells appear to be arrested in S phase. With DHA, the amount of phosphorylated retinoblastoma protein (pRb) decreases significantly. Western...
Articles
Biochem J (2002) 368 (2): 447–459.
Published: 01 December 2002
... to apoptosis. Three proapoptotic responses were observed: the activation of caspase-3, the production of ceramides from newly synthesized pools (as demonstrated by the inhibitor Fumonisin B1) and finally the activation of stress-activated protein kinase, p38 and c-Jun N-terminal kinases 1/2, as a result...
Articles
Biochem J (2002) 365 (1): 69–77.
Published: 01 July 2002
... with the ability of these cells to accumulate ceramide and undergo apoptosis. When DT40 cells were stimulated to apoptose by a variety of agents, including the ER stress, an increase in endogenous ceramide levels was observed. However, these responses were not enhanced compared with another B-cell line (Nalm-6...
Articles
Biochem J (2001) 355 (3): 859–868.
Published: 24 April 2001
...Robert J. VELDMAN; Nicolas MAESTRE; Osama M. ADUIB; Jeffrey A. MEDIN; Robert SALVAYRE; Thierry LEVADE Sphingomyelinases hydrolyse sphingomyelin to ceramide, a process involved in signal-transduction routes leading to apoptosis and various other cellular responses. In the present study, we...
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Biochem J (2000) 350 (3): 747–756.
Published: 08 September 2000
.... Further the pI-4.2 protein purified from extracts of the stratum corneum of ADe patients was able to hydrolyse N -[palmitoyl-1- 14 C]SM and GCer, but not N -[palmitoyl-1- 14 C]ceramide. These results indicate that a hitherto undiscovered epidermal enzyme, termed here glucosylceramide sphingomyelin...
Articles
Biochem J (2000) 346 (3): 671–677.
Published: 07 March 2000
... for SM hydrolysis and SM synthesis are mediated by different catalysts. Our finding that the malaria parasites possess SMase activity might explain why the parasites seem to have an SM synthase activity but no activity to synthesize ceramide de novo . 1 To whom correspondence should be addressed...
Articles
Biochem J (2000) 345 (1): 77–84.
Published: 17 December 1999
...Laura E. BERTELLO; Maria Júlia M. ALVES; Walter COLLI; Rosa M. de LEDERKREMER The lipid moiety in the glycosylphosphatidylinositol anchors of glycoproteins of Trypanosoma cruzi consists of an alkylacylglycerol, a lysoalkylglycerol or a ceramide. Previously, we showed...
Articles
Biochem J (1999) 338 (1): 147–151.
Published: 08 February 1999
... processed, either by degradation (assessed as 3 H 2 O release into the culture medium) or by N-acylation (mainly to radioactive ceramide, sphingomyelin, neutral glycolipids and gangliosides). [ 3 H]Sphingosine 1-phosphate accounted for less than 2% of the total radioactivity incorporated in all cases...