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Articles

The unfolding role of ceramide in coordinating retinoid-based cancer therapy

Botheina Ghandour, Ghassan Dbaibo, Nadine Darwiche

Biochem J (2021) 478 (19): 3621–3642.

https://doi.org/10.1042/BCJ20210368

Published: 14 October 2021

...Botheina Ghandour; Ghassan Dbaibo; Nadine Darwiche Sphingolipid-mediated regulation in cancer development and treatment is largely ceramide-centered with the complex sphingolipid metabolic pathways unfolding as attractive targets for anticancer drug discovery. The dynamic interconversion...

View article titled, The unfolding role of ceramide in coordinating retinoid-based cancer therapy

Articles

Substrate specificity, kinetic properties and inhibition by fumonisin B 1 of ceramide synthase isoforms from Arabidopsis

Kyle D. Luttgeharm, Edgar B. Cahoon, Jonathan E. Markham

Biochem J (2016) 473 (5): 593–603.

https://doi.org/10.1042/BJ20150824

Published: 24 February 2016

...Kyle D. Luttgeharm; Edgar B. Cahoon; Jonathan E. Markham Ceramide makes up the acyl-backbone of sphingolipids and plays a central role in determining the function of these essential membrane lipids. In Arabidopsis, the varied chemical composition of ceramide is determined by the specificity...

View article titled, Substrate specificity, kinetic properties and inhibition by fumonisin B 1 of ceramide synthase isoforms from Arabidopsis

Includes: Supplementary data

Articles

Akt kinase C-terminal modifications control activation loop dephosphorylation and enhance insulin response

Tung O. Chan, Jin Zhang, Brian C. Tiegs, Brian Blumhof, Linda Yan, Nikhil Keny, Morgan Penny, Xue Li, John M. Pascal, Roger S. Armen, Ulrich Rodeck, Raymond B. Penn

Biochem J (2015) 471 (1): 37–51.

https://doi.org/10.1042/BJ20150325

Published: 21 September 2015

... ) increased phosphatase resistance of the phosphorylated activation loop (pThr 308 ) and amplified Akt phosphorylation. Furthermore, the phosphatase-resistant Akt was refractory to ceramide-dependent dephosphorylation and amplified insulin-dependent Thr 308 phosphorylation in a regulated fashion. Collectively...

View article titled, Akt kinase C-terminal modifications control activation loop dephosphorylation and enhance insulin response

Includes: Supplementary data

Articles

Neutral sphingomyelinase-2 is a redox sensitive enzyme: role of catalytic cysteine residues in regulation of enzymatic activity through changes in oligomeric state

P. Patrick Dotson, II, Alexander A. Karakashian, Mariana N. Nikolova-Karakashian

Biochem J (2015) 465 (3): 371–382.

https://doi.org/10.1042/BJ20140665

Published: 22 January 2015

... Society 2015 Neutral sphingomyelinase-2 (nSMase-2) regulates cellular levels of ceramide. This manuscript shows that nSMase-2 is a redox-sensitive enzyme that oligomerizes via Cys 617 -mediated intermolecular bonds that is linked to suppressed enzyme activity, and may be a substrate for thioredoxin...

View article titled, Neutral sphingomyelinase-2 is a redox sensitive enzyme: role of catalytic cysteine residues in regulation of enzymatic activity through changes in oligomeric state

Includes: Supplementary data

Articles

c-Src-induced activation of ceramide metabolism impairs membrane microdomains and promotes malignant progression by facilitating the translocation of c-Src to focal adhesions

Kentaro Kajiwara, Takayuki Yamada, Takeshi Bamba, Eiichiro Fukusaki, Fumio Imamoto, Masato Okada, Chitose Oneyama

Biochem J (2014) 458 (1): 81–93.

https://doi.org/10.1042/BJ20130527

Published: 20 January 2014

... and initiates intracellular signalling cascades that promote malignant transformation, but the underlying mechanisms for c-Src translocation remain unclear. In the present study we show that c-Src up-regulation perturbs sphingolipid/cholesterol-enriched membrane microdomains by activating ceramide synthesis...

View article titled, c-Src-induced activation of ceramide metabolism impairs membrane microdomains and promotes malignant progression by facilitating the translocation of c-Src to focal adhesions

Includes: Supplementary data

Articles

Mitochondrial fission mediates ceramide-induced metabolic disruption in skeletal muscle

Melissa E. Smith, Trevor S. Tippetts, Eric S. Brassfield, Braden J. Tucker, Adelaide Ockey, Adam C. Swensen, Tamil S. Anthonymuthu, Trevor D. Washburn, Daniel A. Kane, John T. Prince, Benjamin T. Bikman

Biochem J (2013) 456 (3): 427–439.

https://doi.org/10.1042/BJ20130807

Published: 22 November 2013

...Melissa E. Smith; Trevor S. Tippetts; Eric S. Brassfield; Braden J. Tucker; Adelaide Ockey; Adam C. Swensen; Tamil S. Anthonymuthu; Trevor D. Washburn; Daniel A. Kane; John T. Prince; Benjamin T. Bikman Ceramide is a sphingolipid that serves as an important second messenger in an increasing number...

View article titled, Mitochondrial fission mediates ceramide-induced metabolic disruption in skeletal muscle

Includes: Supplementary data

Articles

BAK activation is necessary and sufficient to drive ceramide synthase-dependent ceramide accumulation following inhibition of BCL2-like proteins

Levi J. Beverly, Lauren A. Howell, Maria Hernandez-Corbacho, Lavona Casson, Jerry E. Chipuk, Leah J. Siskind

Biochem J (2013) 452 (1): 111–119.

https://doi.org/10.1042/BJ20130147

Published: 25 April 2013

... combinations of drugs that more potently kill cancer cells while sparing normal cells. The BCL2 family of proteins and bioactive sphingolipids are intricately linked during apoptotic cell death. In fact, many chemotherapeutic drugs are known to cause accumulation of the pro-apoptotic sphingolipid ceramide...

View article titled, BAK activation is necessary and sufficient to drive ceramide synthase-dependent ceramide accumulation following inhibition of BCL2-like proteins

Includes: Supplementary data

Articles

Modulation of cellular S1P levels with a novel, potent and specific inhibitor of sphingosine kinase-1

Biochem J (2012) 444 (1): 79–88.

https://doi.org/10.1042/BJ20111929

Published: 26 April 2012

... 3 2012 8 3 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 ceramide sphingosine sphingosine kinase (SphK) sphingosine kinase inhibitor (SphK inhibitor) sphingosine 1-phosphate (S1P) Total RNA was isolated from frozen human 1483 cells using the 6100...

View article titled, Modulation of cellular S1P levels with a novel, potent and specific inhibitor of sphingosine kinase-1

Includes: Supplementary data

Articles

Ceramide synthases at the centre of sphingolipid metabolism and biology

Thomas D. Mullen, Yusuf A. Hannun, Lina M. Obeid

Biochem J (2012) 441 (3): 789–802.

https://doi.org/10.1042/BJ20111626

Published: 16 January 2012

...Thomas D. Mullen; Yusuf A. Hannun; Lina M. Obeid Sphingolipid metabolism in metazoan cells consists of a complex interconnected web of numerous enzymes, metabolites and modes of regulation. At the centre of sphingolipid metabolism reside CerSs (ceramide synthases), a group of enzymes that catalyse...

View article titled, Ceramide synthases at the centre of sphingolipid metabolism and biology

Articles

Mass and relative elution time profiling: two-dimensional analysis of sphingolipids in Alzheimer's disease brains

Leila Hejazi, Jason W.H. Wong, Danni Cheng, Nicholas Proschogo, Diako Ebrahimi, Brett Garner, Anthony S. Don

Biochem J (2011) 438 (1): 165–175.

https://doi.org/10.1042/BJ20110566

Published: 27 July 2011

...) analysis of sphingolipids based on both mass and hydrophobicity, and use this method to characterize the SM (sphingomyelin), ceramide and GalCer (galactosylceramide) content of hippocampus from AD (Alzheimer's disease) and control subjects. Using a mathematical relationship we exclude the influence...

View article titled, Mass and relative elution time profiling: two-dimensional analysis of sphingolipids in Alzheimer's disease brains

Includes: Supplementary data

Articles

Ceramide synthase 4 and de novo production of ceramides with specific N-acyl chain lengths are involved in glucolipotoxicity-induced apoptosis of INS-1 β-cells

Julien Véret, Nicolas Coant, Evgeny V. Berdyshev, Anastasia Skobeleva, Nicole Therville, Danielle Bailbé, Irina Gorshkova, Viswanathan Natarajan, Bernard Portha, Hervé Le Stunff

Biochem J (2011) 438 (1): 177–189.

https://doi.org/10.1042/BJ20101386

Published: 27 July 2011

...Julien Véret; Nicolas Coant; Evgeny V. Berdyshev; Anastasia Skobeleva; Nicole Therville; Danielle Bailbé; Irina Gorshkova; Viswanathan Natarajan; Bernard Portha; Hervé Le Stunff Pancreatic β-cell apoptosis induced by palmitate requires high glucose concentrations. Ceramides have been suggested...

View article titled, Ceramide synthase 4 and de novo production of ceramides with specific N-acyl chain lengths are involved in glucolipotoxicity-induced apoptosis of INS-1 β-cells

Includes: Supplementary data

Articles

Neutral sphingomyelinase 2 (nSMase2) is the primary neutral sphingomyelinase isoform activated by tumour necrosis factor-α in MCF-7 cells

Christopher J. Clarke, Emily A. Cloessner, Patrick L. Roddy, Yusuf A. Hannun

Biochem J (2011) 435 (2): 381–390.

https://doi.org/10.1042/BJ20101752

Published: 29 March 2011

... or untagged nSMase3 in multiple cell lines had no significant effect on in vitro N-SMase activity. Moreover, only overexpression of nSMase2, but not nSMase1 or nSMase3, had significant effects on cellular sphingolipid levels, increasing ceramide and decreasing sphingomyelin. Additionally, only siRNA (small...

View article titled, Neutral sphingomyelinase 2 (nSMase2) is the primary neutral sphingomyelinase isoform activated by tumour necrosis factor-α in MCF-7 cells

Includes: Supplementary data

Articles

A lipidomic screen of palmitate-treated MIN6 β-cells links sphingolipid metabolites with endoplasmic reticulum (ER) stress and impaired protein trafficking

Ebru Boslem, Gemma MacIntosh, Amanda M. Preston, Clarissa Bartley, Anna K. Busch, Maria Fuller, D. Ross Laybutt, Peter J. Meikle, Trevor J. Biden

Biochem J (2011) 435 (1): 267–276.

https://doi.org/10.1042/BJ20101867

Published: 15 March 2011

... by 70% under these conditions, without any significant alteration in the amounts of either ceramide or sphingomyelin. However, flux into ceramide (measured by [ 3 H]serine incorporation) was augmented by chronic palmitate, and inhibition of ceramide synthesis decreased both ER stress and apoptosis. ER...

View article titled, A lipidomic screen of palmitate-treated MIN6 β-cells links sphingolipid metabolites with endoplasmic reticulum (ER) stress and impaired protein trafficking

Includes: Supplementary data

Articles

Dihydroceramide desaturase activity is modulated by oxidative stress

Jolanta Idkowiak-Baldys, Aintzane Apraiz, Li Li, Mehrdad Rahmaniyan, Christopher J. Clarke, Jacqueline M. Kraveka, Aintzane Asumendi, Yusuf A. Hannun

Biochem J (2010) 427 (2): 265–274.

https://doi.org/10.1042/BJ20091589

Published: 29 March 2010

...Jolanta Idkowiak-Baldys; Aintzane Apraiz; Li Li; Mehrdad Rahmaniyan; Christopher J. Clarke; Jacqueline M. Kraveka; Aintzane Asumendi; Yusuf A. Hannun Oxidative stress has been implicated previously in the regulation of ceramide metabolism. In the present study, its effects on dihydroceramide...

View article titled, Dihydroceramide desaturase activity is modulated by oxidative stress

Articles

Disruption of ceramide synthesis by CerS2 down-regulation leads to autophagy and the unfolded protein response

Stefka D. Spassieva, Thomas D. Mullen, Danyelle M. Townsend, Lina M. Obeid

Biochem J (2009) 424 (2): 273–283.

https://doi.org/10.1042/BJ20090699

Published: 11 November 2009

...Stefka D. Spassieva; Thomas D. Mullen; Danyelle M. Townsend; Lina M. Obeid Ceramide metabolism has come under recent scrutiny because of its role in cellular stress responses. CerS2 (ceramide synthase 2) is one of the six mammalian isoforms of ceramide synthase and is responsible for the synthesis...

View article titled, Disruption of ceramide synthesis by CerS2 down-regulation leads to autophagy and the unfolded protein response

Includes: Supplementary data

Articles

Modulating serine palmitoyl transferase (SPT) expression and activity unveils a crucial role in lipid-induced insulin resistance in rat skeletal muscle cells

Maria L. Watson, Matthew Coghlan, Harinder S. Hundal

Biochem J (2009) 417 (3): 791–801.

https://doi.org/10.1042/BJ20081149

Published: 16 January 2009

...Maria L. Watson; Matthew Coghlan; Harinder S. Hundal Saturated fatty acids, such as palmitate, promote accumulation of ceramide, which impairs activation and signalling of PKB (protein kinase B; also known as Akt) to important end points such as glucose transport. SPT (serine palmitoyl transferase...

View article titled, Modulating serine palmitoyl transferase (SPT) expression and activity unveils a crucial role in lipid-induced insulin resistance in rat skeletal muscle cells

Includes: Supplementary data

Articles

Sphingomyelin synthases regulate production of diacylglycerol at the Golgi

Maristella Villani, Marimuthu Subathra, Yeong-Bin Im, Young Choi, Paola Signorelli, Maurizio Del Poeta, Chiara Luberto

Biochem J (2008) 414 (1): 31–41.

https://doi.org/10.1042/BJ20071240

Published: 29 July 2008

...Maristella Villani; Marimuthu Subathra; Yeong-Bin Im; Young Choi; Paola Signorelli; Maurizio Del Poeta; Chiara Luberto SMS [SM (sphingomyelin) synthase] is a class of enzymes that produces SM by transferring a phosphocholine moiety on to ceramide. PC (phosphatidylcholine) is believed...

View article titled, Sphingomyelin synthases regulate production of diacylglycerol at the Golgi

Includes: Supplementary data

Articles

Targeting of PKCζ and PKB to caveolin-enriched microdomains represents a crucial step underpinning the disruption in PKB-directed signalling by ceramide

Eric Hajduch, Sophie Turban, Xavier Le Liepvre, Soazig Le Lay, Christopher Lipina, Nikolaos Dimopoulos, Isabelle Dugail, Harinder S. Hundal

Biochem J (2008) 410 (2): 369–379.

https://doi.org/10.1042/BJ20070936

Published: 12 February 2008

...Eric Hajduch; Sophie Turban; Xavier Le Liepvre; Soazig Le Lay; Christopher Lipina; Nikolaos Dimopoulos; Isabelle Dugail; Harinder S. Hundal Elevated ceramide concentrations in adipocytes and skeletal muscle impair PKB (protein kinase B; also known as Akt)-directed insulin signalling to key hormonal...

View article titled, Targeting of PKCζ and PKB to caveolin-enriched microdomains represents a crucial step underpinning the disruption in PKB-directed signalling by ceramide

Includes: Supplementary data

Articles

Phosphatidylcholine/sphingomyelin metabolism crosstalk inside the nucleus

Elisabetta Albi, Remo Lazzarini, Mariapia Viola Magni

Biochem J (2008) 410 (2): 381–389.

https://doi.org/10.1042/BJ20070758

Published: 12 February 2008

..., which enrich the intranuclear diacylglycerol pool, and the effect of phosphatidylcholine-specific phospholipase C activity on neutral sphingomyelinase and reverse sphingomyelin synthase, which enrich the intranuclear ceramide pool, was investigated. The results show that in chromatin, there exists...

View article titled, Phosphatidylcholine/sphingomyelin metabolism crosstalk inside the nucleus

Articles

Endosomes and lysosomes play distinct roles in sulfatide-induced neuroblastoma apoptosis: potential mechanisms contributing to abnormal sulfatide metabolism in related neuronal diseases

Youchun Zeng, Hua Cheng, Xuntian Jiang, Xianlin Han

Biochem J (2008) 410 (1): 81–92.

https://doi.org/10.1042/BJ20070976

Published: 29 January 2008

... in these disease states remains unknown. In the present study, we investigated the sequelae of NB (neuroblastoma) cells upon sulfatide supplementation and the biochemical mechanisms contributing to the sulfatide-induced changes. By using shotgun lipidomics, we showed dramatic accumulations of sulfatide, ceramide...

View article titled, Endosomes and lysosomes play distinct roles in sulfatide-induced neuroblastoma apoptosis: potential mechanisms contributing to abnormal sulfatide metabolism in related neuronal diseases

Articles

A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1

Claudio Costantini, Mi Hee Ko, Mary Cabell Jonas, Luigi Puglielli

Biochem J (2007) 407 (3): 383–395.

https://doi.org/10.1042/BJ20070040

Published: 12 October 2007

...Claudio Costantini; Mi Hee Ko; Mary Cabell Jonas; Luigi Puglielli The lipid second messenger ceramide regulates the rate of β cleavage of the Alzheimer's disease APP (amyloid precursor protein) by affecting the molecular stability of the β secretase BACE1 (β-site APP cleaving enzyme 1...

View article titled, A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1

Includes: Supplementary data

Articles

Is the mammalian serine palmitoyltransferase a high-molecular-mass complex?

Thorsten Hornemann, Yu Wei, Arnold von Eckardstein

Biochem J (2007) 405 (1): 157–164.

https://doi.org/10.1042/BJ20070025

Published: 13 June 2007

... ml of 0.1 M KCl. 0.5 ml of the lower organic phase was removed and counted using a scintillation counter. ceramide serine palmitoyltransferase (SPT) sphingolipid Sphingolipids are an ubiquitously distributed class of lipids that can be found in all higher organisms. Sphingoid bases...

View article titled, Is the mammalian serine palmitoyltransferase a high-molecular-mass complex?

Articles

Yeast sphingolipids do not need to contain very long chain fatty acids

Vanessa Cerantola, Christine Vionnet, Olivier F. Aebischer, Titus Jenny, Jens Knudsen, Andreas Conzelmann

Biochem J (2007) 401 (1): 205–216.

https://doi.org/10.1042/BJ20061128

Published: 11 December 2006

... for ceramide synthesis by the Lag1p (longevity-assurance gene 1)/Lac1p (longevity-assurance gene cognate 1)/Lip1p (Lag1p/Lac1p interacting protein) ceramide synthase. LAG1 and LAC1 are redundant but LIP1 is essential. Here we show that 4Δ ( lag1 Δ lac1 Δ ypc1 Δ ydc1 Δ) cells devoid of all known endogenous...

View article titled, Yeast sphingolipids do not need to contain very long chain fatty acids

Includes: Supplementary data

Articles

Lack of ceramide generation and altered sphingolipid composition are associated with drug resistance in human ovarian carcinoma cells

Alessandro Prinetti, Danilo Millimaggi, Sandra D'Ascenzo, Matilda Clarkson, Arianna Bettiga, Vanna Chigorno, Sandro Sonnino, Antonio Pavan, Vincenza Dolo

Biochem J (2006) 395 (2): 311–318.

https://doi.org/10.1042/BJ20051184

Published: 28 March 2006

.... This study was undertaken to gain insight into the molecular alterations producing PTX resistance in ovarian cancer. PTX treatment is able to induce apoptosis in the human ovarian carcinoma cell line, CABA I. PTX-induced apoptosis in CABA I cells was accompanied by an increase in the cellular Cer (ceramide...

View article titled, Lack of ceramide generation and altered sphingolipid composition are associated with drug resistance in human ovarian carcinoma cells

Articles

Distinct roles for de novo versus hydrolytic pathways of sphingolipid biosynthesis in Saccharomyces cerevisiae

L. Ashley Cowart, Yasuo Okamoto, Xinghua Lu, Yusuf A. Hannun

Biochem J (2006) 393 (3): 733–740.

https://doi.org/10.1042/BJ20050643

Published: 13 January 2006

...L. Ashley Cowart; Yasuo Okamoto; Xinghua Lu; Yusuf A. Hannun Saccharomyces cerevisiae produces the sphingolipid ceramide by de novo synthesis as well as by hydrolysis of complex sphingolipids by Isc1p (inositolphosphoceramide-phospholipase C), which is homologous with the mammalian neutral...

View article titled, Distinct roles for de novo versus hydrolytic pathways of sphingolipid biosynthesis in Saccharomyces cerevisiae

Includes: Supplementary data

Articles

Regulation of the sphingosine-recycling pathway for ceramide generation by oxidative stress, and its role in controlling c-Myc/Max function

Iyad Sultan, Can E. Senkal, Suriyan Ponnusamy, Jacek Bielawski, Zdzislaw Szulc, Alicja Bielawska, Yusuf A. Hannun, Besim Ogretmen

Biochem J (2006) 393 (2): 513–521.

https://doi.org/10.1042/BJ20051083

Published: 23 December 2005

... of endogenous long-chain ceramide in response to exogenous C 6 -cer (C 6 -ceramide), which is FB1 (fumonisin B1)-sensitive, was employed to probe the sphingosine-recycling pathway. The data showed that ceramide formation via this pathway was significantly blocked by GSH and NAC ( N -acetylcysteine) whereas...

View article titled, Regulation of the sphingosine-recycling pathway for ceramide generation by oxidative stress, and its role in controlling c-Myc/Max function

Articles

Human T-cell lymphotropic virus type I-transformed T-cells have a partial defect in ceramide synthesis in response to N -(4-hydroxyphenyl)retinamide

Nadine Darwiche, Ghada Abou-Lteif, Tarek Najdi, Lina Kozhaya, Ahmad Abou Tayyoun, Ali Bazarbachi, Ghassan S. Dbaibo

Biochem J (2005) 392 (1): 231–239.

https://doi.org/10.1042/BJ20050578

Published: 08 November 2005

...-negative malignant T-cells [ 8 ]. It was observed that HPR-mediated growth inhibition was associated with ceramide accumulation only in HTLV-I-negative cells. The aim of the present study was to investigate the mechanism by which HPR differentially regulates ceramide metabolism in HTLV-I-negative and HTLV...

View article titled, Human T-cell lymphotropic virus type I-transformed T-cells have a partial defect in ceramide synthesis in response to N -(4-hydroxyphenyl)retinamide

Articles

A TrkA-to-p75 NTR molecular switch activates amyloid β-peptide generation during aging

Claudio Costantini, Richard Weindruch, Giuliano Della Valle, Luigi Puglielli

Biochem J (2005) 391 (1): 59–67.

https://doi.org/10.1042/BJ20050700

Published: 26 September 2005

... reduces, whereas p75 NTR activates, β-cleavage of APP. The p75 NTR -dependent effect requires NGF (nerve growth factor) binding and activation of the second messenger ceramide. We also show that normal aging activates Aβ generation in the brain by ‘switching’ from the TrkA to the p75 NTR receptor system...

View article titled, A TrkA-to-p75 NTR molecular switch activates amyloid β-peptide generation during aging

Includes: Supplementary data

Articles

A mammalian fatty acid hydroxylase responsible for the formation of α-hydroxylated galactosylceramide in myelin

Matthias ECKHARDT, Afshin YAGHOOTFAM, Simon N. FEWOU, Inge ZÖLLER, Volkmar GIESELMANN

Biochem J (2005) 388 (1): 245–254.

https://doi.org/10.1042/BJ20041451

Published: 10 May 2005

...Matthias ECKHARDT; Afshin YAGHOOTFAM; Simon N. FEWOU; Inge ZÖLLER; Volkmar GIESELMANN Hydroxylation is an abundant modification of the ceramides in brain, skin, intestinal tract and kidney. Hydroxylation occurs at the sphingosine base at C-4 or within the amide-linked fatty acid. In myelin...

View article titled, A mammalian fatty acid hydroxylase responsible for the formation of α-hydroxylated galactosylceramide in myelin

Articles

Inositol phosphoceramide synthase is a regulator of intracellular levels of diacylglycerol and ceramide during the G 1 to S transition in Saccharomyces cerevisiae

Jorge CERBÓN, Alejandro FALCON, Carlos HERNÁNDEZ-LUNA, David SEGURA-COBOS

Biochem J (2005) 388 (1): 169–176.

https://doi.org/10.1042/BJ20040475

Published: 10 May 2005

...-Sanchez (2003) Biochem. J. 373 , 917–924]. In yeast cells, IPC (inositol phosphoceramide) synthase catalyses the transfer of phosphoinositol from phosphatidylinositol to ceramide to form IPC and generates DAG. In the present study, we found that, during the G 1 to S transition after N 2 -starvation...

View article titled, Inositol phosphoceramide synthase is a regulator of intracellular levels of diacylglycerol and ceramide during the G 1 to S transition in Saccharomyces cerevisiae

Articles

A mitochondrial pool of sphingomyelin is involved in TNFα-induced Bax translocation to mitochondria

Helene BIRBES, Chiara LUBERTO, Yi-Te HSU, Samer EL BAWAB, Yusuf A. HANNUN, Lina M. OBEID

Biochem J (2005) 386 (3): 445–451.

https://doi.org/10.1042/BJ20041627

Published: 08 March 2005

...Helene BIRBES; Chiara LUBERTO; Yi-Te HSU; Samer EL BAWAB; Yusuf A. HANNUN; Lina M. OBEID We recently showed that targeting bSMase (bacterial sphingomyelinase) specifically to mitochondria caused accumulation of ceramide in mitochondria, and induced cytochrome c release and cell death [Birbes, El...

View article titled, A mitochondrial pool of sphingomyelin is involved in TNFα-induced Bax translocation to mitochondria

Articles

Activation of serine/threonine protein phosphatase-1 is required for ceramide-induced survival of sympathetic neurons

Greg PLUMMER, Kathleen R. PERREAULT, Charles F. B. HOLMES, Elena I. POSSE de CHAVES

Biochem J (2005) 385 (3): 685–693.

https://doi.org/10.1042/BJ20040929

Published: 24 January 2005

...Greg PLUMMER; Kathleen R. PERREAULT; Charles F. B. HOLMES; Elena I. POSSE de CHAVES In sympathetic neurons, C 6 -ceramide, as well as endogenous ceramides, blocks apoptosis elicited by NGF (nerve growth factor) deprivation. The mechanism(s) involved in ceramide-induced neuronal survival are poorly...

View article titled, Activation of serine/threonine protein phosphatase-1 is required for ceramide-induced survival of sympathetic neurons

Articles

Subcellular compartmentalization of ceramide metabolism: MAM (mitochondria-associated membrane) and/or mitochondria?

Clara BIONDA, Jacques PORTOUKALIAN, Daniel SCHMITT, Claire RODRIGUEZ-LAFRASSE, Dominique ARDAIL

Biochem J (2004) 382 (2): 527–533.

https://doi.org/10.1042/BJ20031819

Published: 24 August 2004

...Clara BIONDA; Jacques PORTOUKALIAN; Daniel SCHMITT; Claire RODRIGUEZ-LAFRASSE; Dominique ARDAIL Recent studies by our group and others have disclosed the presence of ceramides in mitochondria, and the activities of ceramide synthase and reverse ceramidase in mitochondria have also been reported...

View article titled, Subcellular compartmentalization of ceramide metabolism: MAM (mitochondria-associated membrane) and/or mitochondria?

Articles

Ceramide metabolite, not intact ceramide molecule, may be responsible for cellular toxicity

Kou-Yi TSERNG, Ronda L. GRIFFIN

Biochem J (2004) 380 (3): 715–722.

https://doi.org/10.1042/bj20031733

Published: 15 June 2004

...Kou-Yi TSERNG; Ronda L. GRIFFIN Ceramides, which are produced from the hydrolysis of sphingomyelin or synthesized from serine and palmitate in a de novo pathway, are regarded as important cellular signals for inducing apoptosis. However, controversy over this proposed role of ceramides exists...

View article titled, Ceramide metabolite, not intact ceramide molecule, may be responsible for cellular toxicity

Articles

Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiae

Nils J. FÆRGEMAN, Søren FEDDERSEN, Janne K. CHRISTIANSEN, Morten K. LARSEN, Roger SCHNEITER, Christian UNGERMANN, Kudzai MUTENDA, Peter ROEPSTORFF, Jens KNUDSEN

Biochem J (2004) 380 (3): 907–918.

https://doi.org/10.1042/bj20031949

Published: 15 June 2004

...Nils J. FÆRGEMAN; Søren FEDDERSEN; Janne K. CHRISTIANSEN; Morten K. LARSEN; Roger SCHNEITER; Christian UNGERMANN; Kudzai MUTENDA; Peter ROEPSTORFF; Jens KNUDSEN In the present study, we show that depletion of acyl-CoA-binding protein, Acb1p, in yeast affects ceramide levels, protein trafficking...

View article titled, Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiae

Articles

Ceramide sensitizes astrocytes to oxidative stress: protective role of cannabinoids

Arkaitz CARRACEDO, Math J. H. GEELEN, María DIEZ, Kentaro HANADA, Manuel GUZMÁN, Guillermo VELASCO

Biochem J (2004) 380 (2): 435–440.

https://doi.org/10.1042/bj20031714

Published: 01 June 2004

...Arkaitz CARRACEDO; Math J. H. GEELEN; María DIEZ; Kentaro HANADA; Manuel GUZMÁN; Guillermo VELASCO Cannabinoids induce apoptosis on glioma cells via stimulation of ceramide synthesis de novo , whereas they do not affect viability of primary astrocytes. In the present study, we show that incubation...

View article titled, Ceramide sensitizes astrocytes to oxidative stress: protective role of cannabinoids

Articles

Haematopoietic protein tyrosine phosphatase (HePTP) phosphorylation by cAMP-dependent protein kinase in T-cells: dynamics and subcellular location

Konstantina NIKA, Huong HYUNH, Scott WILLIAMS, Surojit PAUL, Nunzio BOTTINI, Kjetil TASKÉN, Paul J. LOMBROSO, Tomas MUSTELIN

Biochem J (2004) 378 (2): 335–342.

https://doi.org/10.1042/bj20031244

Published: 01 March 2004

.... Activation of PP1 by treatment of the cells with ceramide suppressed Ser-23 phosphorylation, as did transfection of the catalytic subunit of PP1. Phosphorylation at Ser-23 is also increased in a transient manner upon T-cell antigen receptor ligation. In contrast, treatment of cells with phorbol ester had...

View article titled, Haematopoietic protein tyrosine phosphatase (HePTP) phosphorylation by cAMP-dependent protein kinase in T-cells: dynamics and subcellular location

Articles

Analysis of the role of protein kinase B (cAKT) in insulin-dependent induction of glucokinase and sterol regulatory element-binding protein 1 (SREBP1) mRNAs in hepatocytes

Pascale G. RIBAUX, Patrick B. IYNEDJIAN

Biochem J (2003) 376 (3): 697–705.

https://doi.org/10.1042/bj20031287

Published: 15 December 2003

... of PKB by insulin was inhibited by pretreatment of the hepatocytes with C2 ceramide. This resulted in the inhibition of insulin-dependent increases in GCK and SREBP1 mRNAs. A triple mutant of PKB failed to interfere with insulin activation of PKB in hepatocytes even at high overexpression levels achieved...

View article titled, Analysis of the role of protein kinase B (cAKT) in insulin-dependent induction of glucokinase and sterol regulatory element-binding protein 1 (SREBP1) mRNAs in hepatocytes

Articles

Ceramide and glutathione define two independently regulated pathways of cell death initiated by p53 in Molt-4 leukaemia cells

Wissal EL-ASSAAD, Lina KOZHAYA, Sawsan ARAYSI, Shoghag PANJARIAN, Fadi F. BITAR, Elizabeth BAZ, Marwan E. EL-SABBAN, Ghassan S. DBAIBO

Biochem J (2003) 376 (3): 725–732.

https://doi.org/10.1042/bj20030888

Published: 15 December 2003

... of the sphingolipid ceramide and reactive oxygen species are among these pathways. Crossregulation of these two pathways is possible owing to the demonstrated inhibition of neutral sphingomyelinase by glutathione, the predominant cellular antioxidant, and has been observed in some cytokine-dependent cell-death models...

View article titled, Ceramide and glutathione define two independently regulated pathways of cell death initiated by p53 in Molt-4 leukaemia cells

Articles

A neutral ceramidase homologue from Dictyostelium discoideum exhibits an acidic pH optimum

Hatsumi MONJUSHO, Nozomu OKINO, Motohiro TANI, Mineko MAEDA, Motonobu YOSHIDA, Makoto ITO

Biochem J (2003) 376 (2): 473–479.

https://doi.org/10.1042/bj20030652

Published: 01 December 2003

... ceramidase is available on the DNA Data Bank of Japan (DDBJ). 2 5 2003 12 8 2003 28 8 2003 28 8 2003 The Biochemical Society, London ©2003 2003 ceramidase ceramide Dictyostelium discoideum pH dependency sphingolipid Abbreviations used: CDase, ceramidase; Cer...

View article titled, A neutral ceramidase homologue from Dictyostelium discoideum exhibits an acidic pH optimum

Articles

Ceramide induces a loss in cytosolic peroxide levels in mononuclear cells

Darren C. PHILLIPS, Helen R. GRIFFITHS

Biochem J (2003) 375 (3): 567–579.

https://doi.org/10.1042/bj20030693

Published: 01 November 2003

...Darren C. PHILLIPS; Helen R. GRIFFITHS Ceramide (a sphingolipid) and reactive oxygen species are each partly responsible for intracellular signal transduction in response to a variety of agents. It has been reported that ceramide and reactive oxygen species are intimately linked and show reciprocal...

View article titled, Ceramide induces a loss in cytosolic peroxide levels in mononuclear cells

Articles

Membrane disruption and cytotoxicity of hydrophobic N-alkylated imino sugars is independent of the inhibition of protein and lipid glycosylation

Howard R. MELLOR, Frances M. PLATT, Raymond A. DWEK, Terry D. BUTTERS

Biochem J (2003) 374 (2): 307–314.

https://doi.org/10.1042/bj20030348

Published: 01 September 2003

... moiety is coincident with increased compound-induced cytotoxicity. Therefore, in the present study, we examined the mechanism of this cytotoxicity in detail. Despite N -butyl-deoxynojirimycin and N -butyl-deoxygalactonojirimycin inhibiting the glycosylation of ceramide to glucosylceramide, ceramide...

View article titled, Membrane disruption and cytotoxicity of hydrophobic N-alkylated imino sugars is independent of the inhibition of protein and lipid glycosylation

Articles

Cell-cycle arrest in Jurkat leukaemic cells: a possible role for docosahexaenoic acid

Rafat A. SIDDIQUI, Laura J. JENSKI, Kevin A. HARVEY, Jacqueline D. WIESEHAN, William STILLWELL, Gary P. ZALOGA

Biochem J (2003) 371 (2): 621–629.

https://doi.org/10.1042/bj20021667

Published: 15 April 2003

... results in activation of caspase 3, implying induction of apoptosis. DHA (10 μ M) is shown to increase ceramide levels after 6h of incubation and, after 24h, the cells appear to be arrested in S phase. With DHA, the amount of phosphorylated retinoblastoma protein (pRb) decreases significantly. Western...

View article titled, Cell-cycle arrest in Jurkat leukaemic cells: a possible role for docosahexaenoic acid

Articles

A lysophosphatidic acid analogue is revealed as a potent inhibitor of phosphatidylcholine synthesis, inducing apoptosis

Geneviéve GUEGUEN, Virginie GRANCI, Pierre ROGALLE, Fabienne BRIAND-MÉSANGE, Michéle WILSON, Alain KLAÉBÉ, François TERCÉ, Hugues CHAP, Jean-Pierre SALLES, Marie-Françoise SIMON, Frédérique GAITS

Biochem J (2002) 368 (2): 447–459.

https://doi.org/10.1042/bj20020273

Published: 01 December 2002

... to apoptosis. Three proapoptotic responses were observed: the activation of caspase-3, the production of ceramides from newly synthesized pools (as demonstrated by the inhibitor Fumonisin B1) and finally the activation of stress-activated protein kinase, p38 and c-Jun N-terminal kinases 1/2, as a result...

View article titled, A lysophosphatidic acid analogue is revealed as a potent inhibitor of phosphatidylcholine synthesis, inducing apoptosis

Articles

Biochemical identification of a neutral sphingomyelinase 1 (NSM1)-like enzyme as the major NSM activity in the DT40 B-cell line: absence of a role in the apoptotic response to endoplasmic reticulum stress

Amanda C. FENSOME, Michelle JOSEPHS, Matilda KATAN, Fernando RODRIGUES-LIMA

Biochem J (2002) 365 (1): 69–77.

https://doi.org/10.1042/bj20020120

Published: 01 July 2002

... with the ability of these cells to accumulate ceramide and undergo apoptosis. When DT40 cells were stimulated to apoptose by a variety of agents, including the ER stress, an increase in endogenous ceramide levels was observed. However, these responses were not enhanced compared with another B-cell line (Nalm-6...

View article titled, Biochemical identification of a neutral sphingomyelinase 1 (NSM1)-like enzyme as the major NSM activity in the DT40 B-cell line: absence of a role in the apoptotic response to endoplasmic reticulum stress

Articles

A neutral sphingomyelinase resides in sphingolipid-enriched microdomains and is inhibited by the caveolin-scaffolding domain: potential implications in tumour necrosis factor signalling

Robert J. VELDMAN, Nicolas MAESTRE, Osama M. ADUIB, Jeffrey A. MEDIN, Robert SALVAYRE, Thierry LEVADE

Biochem J (2001) 355 (3): 859–868.

https://doi.org/10.1042/bj3550859

Published: 24 April 2001

...Robert J. VELDMAN; Nicolas MAESTRE; Osama M. ADUIB; Jeffrey A. MEDIN; Robert SALVAYRE; Thierry LEVADE Sphingomyelinases hydrolyse sphingomyelin to ceramide, a process involved in signal-transduction routes leading to apoptosis and various other cellular responses. In the present study, we...

View article titled, A neutral sphingomyelinase resides in sphingolipid-enriched microdomains and is inhibited by the caveolin-scaffolding domain: potential implications in tumour necrosis factor signalling

Articles

The skin of atopic dermatitis patients contains a novel enzyme, glucosylceramide sphingomyelin deacylase, which cleaves the N-acyl linkage of sphingomyelin and glucosylceramide

Kazuhiko HIGUCHI, Junko HARA, Reiko OKAMOTO, Makoto KAWASHIMA, Genji IMOKAWA

Biochem J (2000) 350 (3): 747–756.

https://doi.org/10.1042/bj3500747

Published: 08 September 2000

.... Further the pI-4.2 protein purified from extracts of the stratum corneum of ADe patients was able to hydrolyse N -[palmitoyl-1- 14 C]SM and GCer, but not N -[palmitoyl-1- 14 C]ceramide. These results indicate that a hitherto undiscovered epidermal enzyme, termed here glucosylceramide sphingomyelin...

View article titled, The skin of atopic dermatitis patients contains a novel enzyme, glucosylceramide sphingomyelin deacylase, which cleaves the N-acyl linkage of sphingomyelin and glucosylceramide

Articles

Neutral sphingomyelinase activity dependent on Mg2+ and anionic phospholipids in the intraerythrocytic malaria parasite Plasmodium falciparum

Kentaro HANADA, Toshihide MITAMURA, Masayoshi FUKASAWA, Pamela A. MAGISTRADO, Toshihiro HORII, Masahiro NISHIJIMA

Biochem J (2000) 346 (3): 671–677.

https://doi.org/10.1042/bj3460671

Published: 07 March 2000

... for SM hydrolysis and SM synthesis are mediated by different catalysts. Our finding that the malaria parasites possess SMase activity might explain why the parasites seem to have an SM synthase activity but no activity to synthesize ceramide de novo . 1 To whom correspondence should be addressed...

View article titled, Neutral sphingomyelinase activity dependent on Mg2+ and anionic phospholipids in the intraerythrocytic malaria parasite Plasmodium falciparum

Articles

Evidence for phospholipases from Trypanosoma cruzi active on phosphatidylinositol and inositolphosphoceramide

Laura E. BERTELLO, Maria Júlia M. ALVES, Walter COLLI, Rosa M. de LEDERKREMER

Biochem J (2000) 345 (1): 77–84.

https://doi.org/10.1042/bj3450077

Published: 17 December 1999

...Laura E. BERTELLO; Maria Júlia M. ALVES; Walter COLLI; Rosa M. de LEDERKREMER The lipid moiety in the glycosylphosphatidylinositol anchors of glycoproteins of Trypanosoma cruzi consists of an alkylacylglycerol, a lysoalkylglycerol or a ceramide. Previously, we showed...

View article titled, Evidence for phospholipases from Trypanosoma cruzi active on phosphatidylinositol and inositolphosphoceramide

Articles

Predominance of the acylation route in the metabolic processing of exogenous sphingosine in neural and extraneural cells in culture

Laura RIBONI, Rosaria BASSI, Alessandro PRINETTI, Paola VIANI, Guido TETTAMANTI

Biochem J (1999) 338 (1): 147–151.

https://doi.org/10.1042/bj3380147

Published: 08 February 1999

... processed, either by degradation (assessed as 3 H 2 O release into the culture medium) or by N-acylation (mainly to radioactive ceramide, sphingomyelin, neutral glycolipids and gangliosides). [ 3 H]Sphingosine 1-phosphate accounted for less than 2% of the total radioactivity incorporated in all cases...

View article titled, Predominance of the acylation route in the metabolic processing of exogenous sphingosine in neural and extraneural cells in culture

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