...-clamp measurements (i.e. measurements of electrical current flowing through a microscopic membrane patch) on parasitized erythrocytes have revealed a number of discrete single-channel conductances. The selectivity and pharmacological characteristics of these conductances show similarities to the NPP...

... by the presence of threonine side chains in the channel. Finally, the roles of the intracellular metal-binding sites have been assigned to increased thermostability and regulation of the gating. These mechanisms most likely apply to the entire CorA family as they are regulated by the highly conserved amino acids...

..., a dynamic nanomachine, lies at the centre of this process and acts as a protein-conducting channel with a unique property; allowing both forward transfer of secretory proteins but also lateral release into the lipid bilayer with high fidelity and efficiency. This process, tightly orchestrated at the expense...

...Li-Ting Su; Wei Liu; Hsiang-Chin Chen; Omayra González-Pagán; Raymond Habas; Loren W. Runnels TRPM7 (transient receptor potential melastatin 7) is a Ca 2+ - and Mg 2+ -permeant ion channel in possession of its own kinase domain. As a kinase, the protein has been linked to the control of actomyosin...

... in studies of membrane protein refolding. 1 To whom correspondence should be addressed (e-mail [email protected] ). 14 8 2003 5 12 2003 8 12 2003 10 12 2003 The Biochemical Society, London ©2004 2004 β-barrel channel heat-modifiable outer...

... (intracellular Ca 2+ concentration) oscillations, with a period of 1–4 min, which persisted for the duration of recording (20–30 min). Pretreatment with nifedipine, which blocks T- and L-type voltage-operated Ca 2+ channels in spermatogenic cells, did not modify the characteristics of the oscillations...

... in the absence of a Ca 2+ gradient, but more pronounced incremental behaviour when released Ca 2+ builds up at the mouth of the channel. The sensitivity to Ins P 3 is critically dependent on the density of Ins P 3 receptors, so that different stores can respond to different concentration ranges of Ins P 3...

... and was facilitated by a cell-surface protein(s). Within 5min at 37°C, cell-bound Ib generated Na + /K + permeable channels that were blocked by Ia. However, Ib-induced channels or oligomers were not formed at 4°C. Two monoclonal antibodies raised against Ib that recognize unique, neutralizing epitopes within...

... translocated to mitochondria, inserts into the outer membrane, oligomerizes and triggers the release of cytochrome c , possibly by channel formation. The BH3 domain-only protein Bid induces a conformational change in Bax before its insertion into the outer membrane. The mechanism by which Bid promotes Bax...

... changes in NAADP levels. 1 To whom correspondence should be addressed (e-mail [email protected] ). 24 8 2000 26 9 2000 11 10 2000 The Biochemical Society, London © 2000 2000 calcium channel sea urchin egg signal transduction Biochem. J. (2000...