... 2 3 2022 © 2022 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2022 chlorophyll D1 repair non-photochemical quenching photoinhibition It is still under investigation to what extent NPQ is effective in preventing the light-induced...

.... Potential ligands of the [4Fe-4S] cluster (Cys 204 , Cys 208 , Cys 211 ), of SAM (Phe 210 , Glu 308 and Lys 320 ) and of the proposed cobalamin cofactor (Asp 248 , Glu 249 , Leu 29 , Thr 71 , Val 97 ) were identified. BchE chlorophyll Mg-protoporphyrin IX monomethyl ester cyclase radical SAM enzyme...

...Matthew S. Proctor; Marek Pazderník; Philip J. Jackson; Jan Pilný; Elizabeth C. Martin; Mark J. Dickman; Daniel P. Canniffe; Matthew P. Johnson; C. Neil Hunter; Roman Sobotka; Andrew Hitchcock Chlorophyll synthase (ChlG) catalyses a terminal reaction in the chlorophyll biosynthesis pathway...

...Guangyu E. Chen; C. Neil Hunter The unique isocyclic E ring of chlorophylls contributes to their role as light-absorbing pigments in photosynthesis. The formation of the E ring is catalyzed by the Mg-protoporphyrin IX monomethyl ester cyclase, and the O 2 -dependent cyclase in prokaryotes consists...

... in GSB, while also pointing out some areas in need of further investigation. bacteriochlorophyll chlorophyll energy transfer light harvesting near-infrared spectroscopy time-resolved spectroscopy The two BChl molecules in LH1 monomers were extensively characterized prior to any high...

...Michal Gabruk; Beata Mysliwa-Kurdziel; Jerzy Kruk Light-dependent protochlorophyllide oxidoreductase (POR) is a plant enzyme involved in the chlorophyll biosynthesis pathway. POR reduces one of the double bonds of the protochlorophyllide (Pchlide) using NADPH and light. In the present study, we...

...Sarah Hollingshead; Sophie Bliss; Patrick J. Baker; C. Neil Hunter Chlorophylls (Chls) are modified tetrapyrrole molecules, essential for photosynthesis. These pigments possess an isocyclic E ring formed by the Mg-protoporphyrin IX monomethylester cyclase (MgPME–cyclase). We assessed the in vivo...

...Nathan B. P. Adams; Christopher J. Marklew; Pu Qian; Amanda A. Brindley; Paul A. Davison; Per A. Bullough; C. Neil Hunter Magnesium chelatase (MgCH) initiates chlorophyll biosynthesis by catalysing the ATP-dependent insertion of Mg 2+ into protoporphyrin. This large enzyme complex comprises ChlH, I...

...Daniel P. Canniffe; Jack W. Chidgey; C. Neil Hunter Most of the chlorophylls and bacteriochlorophylls utilized for light harvesting by phototrophic organisms carry an ethyl group at the C8 position of the molecule, the product of a C8-vinyl reductase acting on a chlorophyll/bacteriochlorophyll...

.... These genes were subsequently expressed in a cyanobacterial mutant that cannot synthesize C8-ethyl chlorophyll a . R . sphaeroides rsp_3070 was able to restore synthesis of the WT (wild-type) C8-ethyl chlorophyll a in the mutant, whereas bchJ did not. The results of the present study demonstrate that Rsp_3070...

...Nick Sirijovski; Ulf Olsson; Joakim Lundqvist; Salam Al-Karadaghi; Robert D. Willows; Mats Hansson Magnesium chelatase inserts Mg 2+ into protoporphyrin IX and is the first unique enzyme of the chlorophyll biosynthetic pathway. It is a heterotrimeric enzyme, composed of I- (40 kDa), D- (70 kDa...

...Lucien C. D. GIBSON; Poul Erik JENSEN; C. Neil HUNTER The enzyme magnesium-protoporphyrin IX chelatase (Mg chelatase) catalyses the insertion of Mg into protoporphyrin IX, the first committed step in (bacterio)chlorophyll biosynthesis. In the photosynthetic bacterium Rhodobacter sphaeroides...