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Keywords: cholesterol
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Articles
Biochem J (2020) 477 (21): 4243–4261.
Published: 13 November 2020
.... cholesterol glycolipid immune modulation lipid metabolism NKT cell © 2020 The Author(s) 2020 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY-NC-ND) . 30 6...
Articles
Biochem J (2020) 477 (2): 541–555.
Published: 31 January 2020
...Nicola A. Scott; Laura J. Sharpe; Isabelle M. Capell-Hattam; Samuel J. Gullo; Winnie Luu; Andrew J. Brown Cholesterol synthesis is a tightly controlled pathway, with over 20 enzymes involved. Each of these enzymes can be distinctly regulated, helping to fine-tune the production of cholesterol and...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (18): 2545–2560.
Published: 20 September 2019
...Ngee Kiat Chua; Nicola A. Scott; Andrew J. Brown Squalene monooxygenase (SM) is an essential rate-limiting enzyme in cholesterol synthesis. SM degradation is accelerated by excess cholesterol, and this requires the first 100 amino acids of SM (SM N100). This process is part of a protein quality...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (6): 951–963.
Published: 22 March 2019
...Sarah C. Proudfoot; Daisy Sahoo High-density lipoproteins (HDLs) facilitate reverse cholesterol transport, a process in which HDL removes cholesterol from circulation and carries it to the liver for biliary excretion. Reverse cholesterol transport is also facilitated by HDL's high-affinity receptor...
Articles
Biochem J (2018) 475 (24): 3917–3919.
Published: 14 December 2018
...Normand Cyr In a recent issue of Biochemical Journal , Kathuria et al . [ Biochem. J. (2018) 475 , 3039–3055] report that membrane binding of the pore-forming toxin Vibrio cholerae cytolysin (VCC) is facilitated by the presence of cholesterol, and the presence of this sterol within the lipid...
Articles
Biochem J (2018) 475 (19): 3039–3055.
Published: 10 October 2018
... shown that pore formation by VCC requires the presence of cholesterol in the liposome membranes. However, the exact role of cholesterol in the mode of action of VCC still remains unclear. Most importantly, implication of cholesterol, if any, in regulating the pore-formation mechanism of VCC in the...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (10): 1839–1859.
Published: 31 May 2018
...). Changes in brain cholesterol homeostasis have been suggested to affect Aβ metabolism. Cholesterol homeostasis is maintained in the brain by apolipoprotein E (apoE). The apoE4 isoform constitutes the major risk factor for AD. Here, we investigated the effect of apoE forms on Aβ generation and on BACE1...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (3): 621–642.
Published: 09 February 2018
...Lee C. Mangum; Xiang Hou; Abdolsamad Borazjani; Jung Hwa Lee; Matthew K. Ross; J. Allen Crow Macrophage foam cells store excess cholesterol as cholesteryl esters, which need to be hydrolyzed for cholesterol efflux. We recently reported that silencing expression of carboxylesterase 1 (CES1) in human...
Includes: Supplementary data
Articles
Biochem J (2015) 471 (2): 243–253.
Published: 02 October 2015
... cholesterol accumulation in late endosomes (LE) and lysosomes (Ly). Nascent or mutated NPC1 is degraded through the ubiquitin–proteasome pathway, but how NPC1 degradation is regulated remains currently unknown. In the present study, we demonstrated a link between NPC1 degradation and the Akt (protein kinase B...
Includes: Supplementary data
Articles
Biochem J (2015) 469 (2): 177–187.
Published: 06 July 2015
... cholesterol reverses the suppressive effects of salicylate and metformin on cell survival indicating the inhibition of de novo lipogenesis is probably important. Pre-clinical studies evaluating the use of salicylate based drugs alone and in combination with metformin to inhibit de novo lipogenesis and the...
Articles
Biochem J (2015) 469 (2): e9–e11.
Published: 06 July 2015
... 2015 © 2015 Authors; published by Portland Press Limited 2015 1 To whom correspondence should be sent (email baip@med.unideb.hu ). ACBD3 cholesterol ERK fatty acid lipotoxicity PARP PARP [poly(ADP-ribose) polymerase]-1 is a well-characterized stress enzyme that is...
Articles
Biochem J (2015) 467 (3): 495–505.
Published: 17 April 2015
... reflects the MOM phospholipid composition [ 28 – 31 ] and has been shown numerous times to support Bid and Bax-mediated pore formation [ 5 , 10 , 11 , 17 , 54 – 57 ]. Other lipids were added to the membrane as indicated: MLCL, phosphatidylglycerol (PG) and cholesterol. The surface charge of the membrane...
Articles
Biochem J (2015) 465 (2): 305–314.
Published: 06 January 2015
... characterized the ill-defined signal in the TMR. A sequence comparison suggests that the leucine residue of VIL might be part of a CCM (cholesterol consensus motif) that is known to bind cholesterol to seven-transmembrane receptors. The signal also comprises a lysine residue and a tryptophan residue on one and...
Articles
Biochem J (2014) 461 (3): 435–442.
Published: 10 July 2014
...Julian Stevenson; Winnie Luu; Ika Kristiana; Andrew J. Brown SM (squalene mono-oxygenase) catalyses the first oxygenation step in cholesterol synthesis, immediately before the formation of the steroid backbone at lanosterol. SM is an important control point in the pathway, and is regulated at the...
Articles
Biochem J (2014) 459 (2): 301–312.
Published: 28 March 2014
...Paresh P. Chothe; Peter W. Swaan The sodium/bile acid co-transporter ASBT [apical sodium-dependent bile acid transporter; SLC10A2 (solute carrier family 10 member 2)] plays a key role in the enterohepatic recycling of the bile acids and indirectly contributes to cholesterol homoeostasis. ASBT...
Articles
Biochem J (2014) 459 (2): e1–e3.
Published: 28 March 2014
...Nicolas Venteclef; Pascal Ferré Cholesterol plays an indispensable role in regulating the properties of cell membranes in mammalian cells. Accumulation of cholesterol and its intermediates, such as oxysterols, lead to activation of the nuclear receptors LXRs (liver X receptors). LXR is an important...
Articles
Biochem J (2014) 458 (3): 481–489.
Published: 28 February 2014
...Romina F. Vazquez; Sabina M. Maté; Laura S. Bakás; Marisa M. Fernández; Emilio L. Malchiodi; Vanesa S. Herlax Several toxins that act on animal cells present different, but specific, interactions with cholesterol or sphingomyelin. In the present study we demonstrate that HlyA (α-haemolysin) of...
Includes: Supplementary data
Articles
Biochem J (2014) 457 (3): 463–472.
Published: 10 January 2014
... approach, we show that membrane lipids modulate the structure, subcellular localization and activity of FAAH. We report that the FAAH dimer is stabilized by the lipid bilayer and shows a higher membrane-binding affinity and enzymatic activity within membranes containing both cholesterol and the natural...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2014) 457 (1): 99–105.
Published: 10 December 2013
... reticulum). Binding of the LDLR to PCSK9 in the ER promotes autocatalytic cleavage of PCSK9. The cleaved PCSK9 promotes the exit of the LDLR out of the ER. chaperone cholesterol endoplasmic reticulum low-density lipoprotein receptor proprotein convertase subtilisin/kexin type 9 (PCSK9) proteasome...
Includes: Supplementary data
Articles
Biochem J (2013) 450 (2): 387–395.
Published: 15 February 2013
... during solubilization, whereas a detergent-induced dissociation of the ABCG2 dimer causes an irreversible inactivation. By using the purified and reconstituted protein we demonstrate that cholesterol is an essential activator, whereas bile acids are important modulators of ABCG2 activity. Both wild-type...
Includes: Supplementary data
Articles
Biochem J (2012) 447 (2): 301–311.
Published: 26 September 2012
... reductase and the synthesis of cholesterol in cardiomyocytes, their main target cells. Surprisingly this did not promote intracellular cholesterol accumulation. The glycosides activated the liver X receptor transcription factor and increased the expression of ABCA1 (ATP-binding cassette protein A1...
Includes: Supplementary data
Articles
Biochem J (2012) 447 (1): 51–60.
Published: 12 September 2012
...Maaike Kockx; Donna L. Dinnes; Kuan-Yen Huang; Laura J. Sharpe; Wendy Jessup; Andrew J. Brown; Leonard Kritharides Cholesterol excess is typical of various diseases including atherosclerosis. We have investigated whether cholesterol accumulation in the ER (endoplasmic reticulum) can inhibit exit of...
Includes: Supplementary data
Articles
Biochem J (2012) 446 (2): 191–201.
Published: 14 August 2012
...James R. Krycer; Lisa Phan; Andrew J. Brown There is growing evidence showing that prostate cancer cells have perturbed cholesterol homoeostasis, accumulating cholesterol to promote cell growth. Consequently, cholesterol-lowering drugs such as statins are being evaluated in prostate cancer...
Includes: Supplementary data
Articles
Biochem J (2011) 439 (3): 497–504.
Published: 13 October 2011
... possible regulators of this process in the direct and indirect apical trafficking pathways respectively. To test this possibility, we took a biochemical approach. We determined that MAL, but not MAL2, self-associates, forms higher-order cholesterol-dependent complexes with apical proteins and promotes the...
Articles
Biochem J (2011) 437 (3): 541–553.
Published: 13 July 2011
...Sabine Muth; Anja Fries; Gerald Gimpl Recent studies suggest that cholesterol binding is widespread among GPCRs (G-protein-coupled receptors). In the present study, we analysed putative cholesterol-induced changes in the OTR [OT (oxytocin) receptor], a prototype of cholesterol-interacting GPCRs...
Includes: Supplementary data
Articles
Biochem J (2011) 437 (1): 13–24.
Published: 14 June 2011
... gwyn.gould@glasgow.ac.uk ) 25 1 2011 23 2 2011 1 3 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 cholesterol cytokinesis cytoskeleton membrane trafficking phospholipid soluble N -ethylmaleimide-sensitive fusion protein-attachment protein receptor...
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Biochem J (2010) 429 (2): 235–242.
Published: 28 June 2010
... To whom correspondence should be addressed (email elina.ikonen@helsinki.fi ). 23 2 2010 14 4 2010 6 5 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 cholesterol imaging lipid lipidosis model organism zebrafish The number of diseases...
Articles
Biochem J (2010) 429 (1): 13–24.
Published: 14 June 2010
...Mike H. Ngo; Terry R. Colbourne; Neale D. Ridgway Cholesterol and its numerous oxygenated derivatives (oxysterols) profoundly affect the biophysical properties of membranes, and positively and negatively regulate sterol homoeostasis through interaction with effector proteins. As the bulk of...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2010) 428 (1): 95–101.
Published: 28 April 2010
... efficiently than mock-treated PrP Sc . Whereas the addition of PrP Sc increased cellular cholesterol levels and was predominantly found within lipid raft micro-domains, PrP Sc -G-lyso-PI did not alter cholesterol levels and most of it was found outside lipid rafts. We conclude that the nature of the GPI...
Articles
Biochem J (2009) 424 (2): e5–e6.
Published: 11 November 2009
...’. Model membrane studies revealed that these lipid rafts are related to the more ordered liquid phase that forms in a ternary mixture of cholesterol with a phospholipid containing saturated acyl chains and one with unsaturated acyl chains. Giant plasma membrane vesicles that pinch off from cells undergo...
Articles
Biochem J (2009) 423 (1): 1–14.
Published: 14 September 2009
... literature on protein and lipid roles in exocytosis, with emphasis on the multiple roles of cholesterol in exocytosis and membrane fusion, in an effort to promote a more molecular systems-level view of the as yet poorly understood process of Ca 2+ -triggered membrane mergers. 1 To whom correspondence...
Articles
Biochem J (2009) 420 (3): 373–381.
Published: 27 May 2009
... remodelling of the PM, including a rapid rearrangement of the glycosphingolipid GM1 and cholesterol into newly formed structures, only partial solubilization of fluid domains and the formation of condensed domains that cover 51% of the remaining membrane. TX does not appear to induce the coalescence of pre...
Articles
Biochem J (2009) 420 (2): 179–191.
Published: 13 May 2009
... delivery vectors. In the present study, qualitative and quantitative analysis of the influence of temperature, peptide concentration and plasma membrane cholesterol on the uptake and subcellular distribution of the model cell-penetrating peptide octa-arginine was performed in a number of suspension and...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2009) 419 (3): 577–584.
Published: 14 April 2009
... cellular LDLc (LDL-cholesterol) and a concomitant increase in serum LDLc. Central to the function of PCSK9 is a direct protein–protein interaction formed with the LDLR. In the present study, we investigated a strategy to modulate LDL uptake by blocking this interaction using specific antibodies directed...
Articles
Biochem J (2009) 418 (2): 369–378.
Published: 11 February 2009
...) are essential for survival inside of host macrophages. These genes act as an operon and have been suggested to be involved in cholesterol metabolism. However, the role of NAT in this catabolic pathway has not been determined. In an effort to better understand the function of these proteins, we have...
Includes: Supplementary data
Articles
Biochem J (2009) 418 (1): 209–217.
Published: 28 January 2009
...Karsten Gehrig; Thomas A. Lagace; Neale D. Ridgway In addition to suppressing cholesterol synthesis and uptake, oxysterols also activate glycerophospholipid and SM (sphingomyelin) synthesis, possibly to buffer cells from excess sterol accumulation. In the present study, we investigated the effects...
Articles
Biochem J (2009) 417 (2): 525–533.
Published: 23 December 2008
... to chelation of cholesterol, maintain outside-out orientation of membrane glycoproteins, have prolonged (18 h) stability at 37 °C, and are vesicles or sheets up to 150–200 nm diameter. DRMs containing GPI (glycosylphosphatidylinositol)-anchored proteins PrP (prion protein) and Thy-1 can be separated...
Includes: Supplementary data
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Biochem J (2008) 415 (1): 87–96.
Published: 12 September 2008
... Toxoplasma cells and their purified rhoptries. This comparative lipidomic analysis revealed an enrichment of cholesterol, sphingomyelin and, most of all, saturated fatty acids in the rhoptries. These lipids are known, when present in membranes, to contribute to their rigidity and, interestingly, fluorescence...
Includes: Supplementary data
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Biochem J (2008) 412 (3): 553–562.
Published: 28 May 2008
...Alireza Roostaee; Élie Barbar; Jean-Guy LeHoux; Pierre Lavigne Steroidogenesis depends on the delivery of cholesterol from the outer to the inner mitochondrial membrane by StAR (steroidogenic acute regulatory protein). However, the mechanism by which StAR binds to cholesterol and its importance in...
Includes: Supplementary data
Articles
Biochem J (2008) 410 (2): 391–400.
Published: 12 February 2008
... 2008 cholesterol computational modelling mutagenesis structure/function Efficient conservation of the bile acid pool occurs via multiple active transporters expressed along the route of the enterohepatic circulation. Among these integral membrane proteins, ASBT (apical Na...
Articles
Biochem J (2007) 405 (3): 473–480.
Published: 13 July 2007
...]25OH (25-hydroxycholesterol)-binding assay with purified recombinant proteins as well as live cell photo-cross-linking with [ 3 H]photo-25OH and [ 3 H]photoCH (photo-cholesterol), to investigate sterol binding by the mammalian ORPs. ORP1 and ORP2 [a short ORP consisting of an ORD (OSBP-related ligand...
Articles
Biochem J (2007) 403 (1): 167–175.
Published: 13 March 2007
... (parasitophorous vacuole) that separates the host cell cytoplasm from the parasite surface. The pore-forming toxin, SLO (streptolysin O), binds to cholesterol-containing membranes and can be used to selectively permeabilize the host cell membrane while leaving the PV membrane intact. We found that in mixtures of...
Includes: Multimedia, Supplementary data
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Biochem J (2007) 402 (1): 117–124.
Published: 25 January 2007
... lipid-free/lipid-poor form and promotes ABCA1 (ATP-binding cassette transporter A1)-dependent cellular cholesterol efflux. In contrast with lipid-free apoA-I and apoE, lipid-free SAA was recently reported to mobilize SR-BI (scavenger receptor class B, type I)-dependent cellular cholesterol efflux [Van...
Articles
Biochem J (2007) 401 (2): 541–549.
Published: 21 December 2006
... raft-dependent internalization of ALP and induction of apoptosis. Similar results were obtained by treatment of the cells with myriocin/ISP-1, an inhibitor of general sphingolipid synthesis, or with U18666A, a cholesterol homoeostasis perturbing agent. U18666A is known to inhibit Niemann–Pick C1...
Articles
Biochem J (2007) 401 (2): 597–605.
Published: 21 December 2006
... the lipid bilayer and that cholesterol in the bilayer also interacts with MDR1. However, the effects of cholesterol on drug–MDR1 interactions are still unclear. To examine these effects, human MDR1 was expressed in insect cells and purified. The purified MDR1 protein was reconstituted in...
Articles
Biochem J (2006) 399 (2): 205–214.
Published: 27 September 2006
...-secretase and its trafficking into cholesterol-rich microdomains. As statins influence these lipid modifications in addition to their effects on cholesterol biosynthesis, we have investigated the effects of lovastatin and SIMVA (simvastatin) at a range of concentrations chosen to distinguish different...
Articles
Biochem J (2006) 398 (3): 485–495.
Published: 29 August 2006
... of proton leak phenomena. We have used stopped-flow fluorimetry to probe the influence of two lipid raft components, chol (cholesterol) and SM (sphingomyelin), on H + /OH − and water permeability. Increasing the concentrations of both lipids in POPC (palmitoyl-2-oleoyl phosphatidylcholine) liposomes...
Articles
Biochem J (2006) 398 (3): 423–430.
Published: 29 August 2006
... normally and did not show significant difference in the levels of cholesterol, BAs or any other bile constituents. However, they did not form cholesterol gallstones when fed a cholic acid-containing high-fat diet, and liver-specific gene expression indicated that Gpbar1-deficient mice have altered feedback...
Includes: Supplementary data
Articles
Biochem J (2006) 397 (3): 407–416.
Published: 13 July 2006
...Eduard B. Babiychuk; Annette Draeger Lateral segregation of cholesterol- and sphingomyelin-rich rafts and glycerophospholipid-containing non-raft microdomains has been proposed to play a role in a variety of biological processes. The most compelling evidence for membrane segregation is based on the...
Articles
Biochem J (2005) 392 (1): 29–38.
Published: 08 November 2005
... with the raft marker flotillin-2, but not caveolin-1, in high-density gradient fractions. The integrity of the polycystin multiprotein complex was sensitive to cholesterol depletion, as shown by cyclodextrin treatment of immunoprecipitated complexes. The overexpressed C-terminus of polycystin-1...
Includes: Supplementary data