... revealed sedlin to be predominantly expressed in proliferating and hypertrophic chondrocytes. Cell culture studies showed that the wild-type protein localized predominantly in the vicinity of the nucleus and the Golgi, with further localization around the cytoplasm, whereas mutation resulted...

... a yeast two-hybrid screening using CCN2/CTGF peptide as a bait and a cDNA library from a chondrocytic cell line, HCS-2/8. In the present paper, we report the identification of aggrecan, which is a major proteoglycan of the extracellular matrix in cartilage, as a CCN2/CTGF-binding protein. Among the four...

...Simon R. Tew; Peraphan Pothacharoen; Theoni Katopodi; Timothy E. Hardingham The transcription factor SOX9 (Sry-type high-mobility-group box 9) is expressed in all chondrocytes and is essential for the expression of aggrecan, which during biosynthesis is substituted with more than 10 times its...

...Bela G. Starkman; John D. Cravero; Marcello Delcarlo, Jr; Richard F. Loeser The IGF-I (insulin-like growth factor-I) signalling pathway responsible for regulation of proteoglycan synthesis in chondrocytes has not been defined and is the focus of the present study. Chondrocytes isolated from normal...

... expression via RARs (retinoic acid receptors) working as heterodimers with RXRs (retinoid X receptors). RXRs also heterodimerize with other nuclear receptors. In this issue of the Biochemical Journal , Harris et al. have shown that an enhancer responsible for chondrocyte-specific expression of the col11a2...

... pathways and production of MMPs (matrix metalloproteases) and chemokines. Stimulation of human skin fibroblasts or articular chondrocytes with IL-1 or TNF-α in the presence of HC-gp39 resulted in a marked reduction of both p38 mitogen-activated protein kinase and stress-activated protein kinase/Jun N...

...Lee-Anne STANTON; Shalev SABARI; Arthur V. SAMPAIO; T. Michael UNDERHILL; Frank BEIER Longitudinal growth of endochondral bones is accomplished through the co-ordinated proliferation and hypertrophic differentiation of growth plate chondrocytes. The molecular mechanisms and signalling cascades...

...Steven P. LUCKMAN; Elaine REES; Alvin P. L. KWAN Type X collagen is a short-chain non-fibrillar collagen that is deposited exclusively at sites of new bone formation. Although this collagen has been implicated in chondrocyte hypertrophy and endochondral ossification, its precise function remains...

...Anneliese D. RECKLIES; Chantal WHITE; Hua LING Human cartilage glycoprotein 39 (HC-gp39) is a glycoprotein secreted by articular chondrocytes, synoviocytes and macrophages. Increased levels of HC-gp39 have been demonstrated in synovial fluids of patients with rheumatoid or osteoarthritis...

...Cordula MAGEE; Maria NURMINSKAYA; Thomas F. LINSENMAYER Previously, we have performed subtractive hybridization to identify genes up-regulated in hypertrophic chondrocytes of the avian epiphyseal growth plate. In the present study, we report the identification of one of the clones as UDP-glucose...

...Heather BRYSON; David J. BUTTLE; L. Didem KOZACI; Rachel N. JOHNATTY; Rowena A. D. BUNNING The effect of mannosamine, an inhibitor of glycosylphosphatidylinositol (GPI) anchor formation, on chondrocyte-mediated cartilage proteoglycan breakdown was investigated using cartilage explant cultures...

... the structural organization and biomechanical properties of cartilage matrix or regulation of a chondrocytic phenotype. 1 To whom correspondence should be addressed (e-mail [email protected]). 3 12 1998 12 4 1999 25 5 1999 The Biochemical Society, London © 1999 1999 chondrocyte...