... -endosulfatase, such chondroitin sulfate (CS)/dermatan sulfate (DS) sulfatase is a useful tool for exploring the structure–function relationship of CS/DS. A commercial preparation (200 mg) of CS-A and CS-E were partially digested with 0.1 U endolyase HCLase, and the resulting oligosaccharides were...

... of proteoglycans (PGs). Among these, chondroitin sulfate (CS) and dermatan sulfate, side chains of PGs, are widely distributed in animal kingdom and show structural variations, such as sulfation patterns and degree of epimerization, which are responsible for their physiological functions through interactions...

...Kyohei Higashi; Keita Takeda; Ann Mukuno; Yusuke Okamoto; Sayaka Masuko; Robert J. Linhardt; Toshihiko Toida Glycosaminoglycans (GAGs), including chondroitin sulfate (CS), dermatan sulfate, heparin, heparan sulfate and keratan sulfate (KS) are linear sulfated repeating disaccharide sequences...

... To whom correspondence should be addressed (email [email protected] ). 28 2 2013 21 5 2013 4 6 2013 4 6 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 chondroitin sulfate exostosin-like 2 (EXTL2) glycosaminoglycan heparan sulfate...

...Tomomi Izumikawa; Toshiyasu Koike; Hiroshi Kitagawa Recently, it has been shown that a deficiency in ChGn-1 (chondroitin N -acetylgalactosaminyltransferase-1) reduced the numbers of CS (chondroitin sulfate) chains, leading to skeletal dysplasias in mice. Although these results indicate that ChGn-1...

...Tomomi Izumikawa; Yuka Okuura; Toshiyasu Koike; Naoki Sakoda; Hiroshi Kitagawa Previously, we demonstrated that sog9 cells, a murine L cell mutant, are deficient in the expression of C4ST (chondroitin 4-O-sulfotransferase)-1 and that they synthesize fewer and shorter CS (chondroitin sulfate) chains...

... Attribution Non-Commercial Licence ( http://creativecommons.org/licenses/by-nc/2.5/ ) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. cartilage chondroitin sulfate collagen fibre N...

... of cathepsin K that exploit the conformational flexibility of the enzyme to regulate its activity and stability against autoproteolysis. All sulfated glycosaminoglycans act as non-essential activators in assays using low-molecular-mass substrates. Chondroitin sulfate and dermatan sulfate bind at one site...

... of FAM20B increased the amount of both chondroitin sulfate and heparan sulfate in HeLa cells, whereas the RNA interference of FAM20B resulted in a reduction of their amount in the cells. Gel-filtration analysis of the glycosaminoglycan chains synthesized in the overexpressing cells revealed...

...Shuji Mizumoto; Tadahisa Mikami; Daiki Yasunaga; Naoki Kobayashi; Hajime Yamauchi; Ayumi Miyake; Nobuyuki Itoh; Hiroshi Kitagawa; Kazuyuki Sugahara CS (chondroitin sulfate) has been implicated in a variety of biological processes during development. Its biological functions are closely associated...

...Frøy Grøndahl; Heidi Tveit; Kristian Prydz PGs (proteoglycans) are proteins acquiring long, linear and sulfated GAG (glycosaminoglycan) chains during Golgi passage. In MDCK cells (Madin–Darby canine kidney cells), most of the CS (chondroitin sulfate) PGs are secreted apically, whereas most...

... of the invariant chain cytosolic domain sequences, mediated through assembly of invariant chain into homotrimers. A small subset of invariant chain is modified by the addition of chondroitin sulfate and is expressed on the cell surface in association with MHCII. In the present study, we have followed...

... matrix. These proteins exhibit very diverse conformations and supramolecular assemblies. Type XV collagen is a large macromolecule distinguished by a highly interrupted collagenous domain and many utilized sites of attachment for CS (chondroitin sulfate) and HS (heparan sulfate) glycosaminoglycan chains...

... of the chondroitin polymerase activity of ChSy-1. These results prompted us to evaluate the effects of co-expression of the recently cloned CSS3 (chondroitin sulfate synthase-3) with ChPF, because ChSy-1 and CSS3 have similar properties, i.e. they possess GalNAcT-II ( N -acetylgalactosaminyltransferase-II) and GlcAT...

...Joji Iida; Krista L. Wilhelmson; Janet Ng; Peter Lee; Charlotte Morrison; Eric Tam; Christopher M. Overall; James B. McCarthy We previously reported that CS (chondroitin sulfate) GAG (glycosaminoglycan), expressed on MCSP (melanoma-specific CS proteoglycan), is important for regulating MT3-MMP...