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1-27 of 27
Keywords: circular dichroism
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Articles
Journal:
Biochemical Journal
Biochem J (2022) 479 (5): 687–700.
Published: 16 March 2022
.... However, it was unclear whether and how phosphorylation could stabilize a helix in a broader context. In the current manuscript, we address this challenge using poly-Ala-based model peptides and a suite of circular dichroism and nuclear magnetic resonance spectroscopies. We show that phosphorylation...
Includes: Supplementary data
Articles
Excitation energy transfer kinetics of trimeric, monomeric and subunit-depleted Photosystem I from Synechocystis PCC 6803
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2021) 478 (7): 1333–1346.
Published: 06 April 2021
... 3 2021 9 3 2021 9 3 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 circular dichroism cyanobacteria fluorescence resonance energy transfer photosynthesis time-resolved spectroscopy Photosystem I (PSI...
Includes: Supplementary data
Articles
Macroorganisation and flexibility of thylakoid membranes
Available to PurchaseIn Collection
Photosynthesis
Journal:
Biochemical Journal
Biochem J (2019) 476 (20): 2981–3018.
Published: 28 October 2019
...–protein assemblies, aka macroorganisation, and its dynamic responses to the fluctuating physiological environment, aka flexibility, are the subject of this review. An emphasis is given on the information obtainable by spectroscopic approaches, especially circular dichroism (CD). We briefly summarise...
Articles
Caspase-9 CARD : core domain interactions require a properly formed active site
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2018) 475 (6): 1177–1196.
Published: 29 March 2018
... 27 2 2018 1 3 2018 2 3 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 apoptosis CARD caspase-9 circular dichroism interdomain interactions protein conformation Apoptosis or programmed cell death...
Includes: Supplementary data
Articles
Spectroscopic and calorimetric characterization of spermine oxidase and its association forms
Available to PurchaseAlessia Leonetti, Laura Cervoni, Fabio Polticelli, Yuta Kanamori, Zuleyha Nihan Yurtsever, Enzo Agostinelli, Paolo Mariottini, Pasquale Stano, Manuela Cervelli
Journal:
Biochemical Journal
Biochem J (2017) 474 (24): 4253–4268.
Published: 14 December 2017
... spectroscopic methods (circular dichroism, fluorescence) and thermal methods (differential scanning calorimetry), we provide new insights into the structure, the transformation, and the stability of SMOX. While the crystal structure of this protein is not available yet, experimental results are interpreted also...
Articles
Eva I. Hyde, Philip Callow, Karthik V. Rajasekar, Peter Timmins, Trushar R. Patel, Giuliano Siligardi, Rohanah Hussain, Scott A. White, Christopher M. Thomas, David J. Scott
Journal:
Biochemical Journal
Biochem J (2017) 474 (18): 3121–3135.
Published: 31 August 2017
...-operatively with other proteins, including the repressor KorA. Like many multifunctional proteins, KorB contains regions of intrinsically disordered structure, existing in a large ensemble of interconverting conformations. Using NMR spectroscopy, circular dichroism and small-angle neutron scattering, we...
Includes: Supplementary data
Articles
Catecholaminergic polymorphic ventricular tachycardia-related mutations R33Q and L167H alter calcium sensitivity of human cardiac calsequestrin
Available to PurchaseGiorgia Valle, Daniela Galla, Alessandra Nori, Silvia G. Priori, Sandor Gyorke, Vincenzo de Filippis, Pompeo Volpe
Journal:
Biochemical Journal
Biochem J (2008) 413 (2): 291–303.
Published: 26 June 2008
... or [email protected] ). 21 1 2008 8 4 2008 9 4 2008 9 4 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 calcium binding calsequestrin circular dichroism fluorescence protein stability proteolysis The homogeneity of CASQ2 proteins...
Articles
Cholesterol binding is a prerequisite for the activity of the steroidogenic acute regulatory protein (StAR)
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2008) 412 (3): 553–562.
Published: 28 May 2008
... ). 13 9 2007 13 3 2008 17 3 2008 17 3 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 cholesterol circular dichroism mitochondria steroidogenesis steroidogenic acute regulatory protein (StAR) thermodynamics Recombinant N-62 StAR...
Includes: Supplementary data
Articles
Role of Ser 11 in the stabilization of the structure of Ochrobactrum anthropi glutathione transferase
Available to PurchaseLuca Federici, Michele Masulli, Daniele Bonivento, Adele Di Matteo, Stefano Gianni, Bartolo Favaloro, Carmine Di Ilio, Nerino Allocati
Journal:
Biochemical Journal
Biochem J (2007) 403 (2): 267–274.
Published: 26 March 2007
... 2007 1 To whom correspondence should be addressed (email [email protected] ). bacterial glutathione transferase (bacterial GST) circular dichroism crystallography G-site Ochrobactrum anthropi unfolding GSTs (glutathione transferases) (EC 2.5.1.18) are a family...
Articles
Inhibition of a metal-dependent viral RNA triphosphatase by decavanadate
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2006) 398 (3): 557–567.
Published: 29 August 2006
... virus circular dichroism denaturation studies enzymology fluorescence spectroscopy Hydrolysis of the γ-phosphate of nascent mRNAs by RNA triphosphatases constitutes the first step in the synthesis of the cap structure found at the 5′ end of eukaryotic mRNAs [ 1 ]. The cap structure...
Articles
Mapping and conformational characterization of the DNA-binding region of the breast cancer susceptibility protein BRCA1
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2006) 395 (3): 529–535.
Published: 11 April 2006
... breast cancer susceptibility protein 1 (BRCA1) circular dichroism DNA-binding domain four-way junction DNA limited proteolysis The BRCA1 gene encodes a large protein of 1863 amino acids that functions as a scaffold co-ordinating protein–protein interactions and/or post-translational...
Articles
Structural and functional studies of vertebrate metallothioneins: cross-talk between domains in the absence of physical contact
Available to PurchaseClemente Capasso, Vincenzo Carginale, Orlando Crescenzi, Daniela Di Maro, Roberta Spadaccini, Piero Andrea Temussi, Elio Parisi
Journal:
Biochemical Journal
Biochem J (2005) 391 (1): 95–103.
Published: 26 September 2005
... MT than for its mammalian counterpart [ 12 , 23 ]. circular dichroism metallothionein metal mobility NMR thiol reactivity Zinc and copper are physiologically important nutrients for animals [ 1 ] and plants [ 2 ], providing essential structural and catalytic functions to a large...
Articles
A novel assay method for an amino acid racemase reaction based on circular dichroism
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2005) 389 (2): 491–496.
Published: 05 July 2005
...Masafumi Noda; Yasuyuki Matoba; Takanori Kumagai; Masanori Sugiyama We have established a novel assay method based on circular dichroism that can be used for the kinetic study of the activity of amino acid racemases, such as ALR (alanine racemase). Although an enzyme-coupled assay method has been...
Articles
TraA and its N-terminal relaxase domain of the Gram-positive plasmid pIP501 show specific oriT binding and behave as dimers in solution
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2005) 387 (2): 401–409.
Published: 05 April 2005
.... The protein solutions were diluted for subsequent experiments. aggregation state circular dichroism DNA–protein interaction plasmid pIP501 relaxase small-angle X-ray scattering For the SAXS measurements, an evacuated high-performance SAXS instrument, ‘SAXSess’ (Anton Paar KG, Graz...
Includes: Supplementary data
Articles
Glutamic acid-65 is an essential residue for catalysis in Proteus mirabilis glutathione S-transferase B1-1
Available to PurchaseNerino ALLOCATI, Michele MASULLI, Enrico CASALONE, Silvia SANTUCCI, Bartolo FAVALORO, Michael W. PARKER, Carmine DI ILIO
Journal:
Biochemical Journal
Biochem J (2002) 363 (1): 189–193.
Published: 22 March 2002
... correspondence should be addressed (e-mail [email protected] ). 24 10 2001 12 12 2001 28 1 2002 The Biochemical Society, London ©2002 2002 circular dichroism GST site-directed mutagenesis Abbreviations used: CDNB, 1-chloro-2,4-dinitrobenzene; GST, glutathione S...
Articles
Evolution of maurotoxin conformation and blocking efficacy towards Shaker B channels during the course of folding and oxidation in vitro
Available to PurchaseEric DI LUCCIO, Alessandra MATAVEL, Sandrine OPI, Imed REGAYA, Guillaume SANDOZ, Sarrah M'BAREK, Edmond CARLIER, Eric ESTÈVE, Louis CARREGA, Ziad FAJLOUN, Hervé ROCHAT, Erwann LORET, Michel DE WAARD, Jean-Marc SABATIER
Journal:
Biochemical Journal
Biochem J (2002) 361 (2): 409–416.
Published: 08 January 2002
... and alter the bioactive three-dimensional structure of sMTX. Key words: circular dichroism, half-cystine pairing, K+ channel, mass spectrometry, scorpion toxin. solubilization in a suitable oxidation buffer. Secondly, we noticed that changes in the three-dimensional (3-D) structure and in the pattern...
Articles
Hormone-triggered conformational changes within the insulin-receptor ectodomain: requirement for transmembrane anchors
Available to PurchaseRalf-Rudiger FLÖRKE, Kerstin SCHNAITH, Waltraud PASSLACK, Marc WICHERT, Lothar KUEHN, Marlies FABRY, Matthias FEDERWISCH, Hans REINAUER
Journal:
Biochemical Journal
Biochem J (2001) 360 (1): 189–198.
Published: 08 November 2001
... be addressed (e-mail [email protected] ). 2 This department is now named Abteilung für Klinische Biochemie und Pathobiochemie. 1 6 2001 16 8 2001 12 9 2001 The Biochemical Society, London ©2001 2001 circular dichroism fluorescence spectroscopy...
Articles
Structural characterization and thermal stability of Notothenia coriiceps metallothionein
Available to PurchaseSabato D'AURIA, Vincenzo CARGINALE, Rosaria SCUDIERO, Orlando CRESCENZI, Daniela DI MARO, Piero Andrea TEMUSSI, Elio PARISI, Clemente CAPASSO
Journal:
Biochemical Journal
Biochem J (2001) 354 (2): 291–299.
Published: 22 February 2001
... spectroscopy circular dichroism NMR temperature effect zinc mobility Biochem. J. (2001) 354, 291 299 (Printed in Great Britain) 291 Structural characterization and thermal stability of Notothenia coriiceps metallothionein1 Sabato D AURIA*2, Vincenzo CARGINALE*, Rosaria SCUDIERO, Orlando CRESCENZI...
Articles
Studies on a single immunoglobulin-binding domain of protein L from Peptostreptococcus magnus: the role of tyrosine-53 in the reaction with human IgG
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2001) 353 (2): 395–401.
Published: 08 January 2001
... that the phenol group of Tyr 53 in PpL is important to the stability of the PpLŐκ-chain complex. 1 To whom correspondence should be addressed (e-mail [email protected] ). 4 7 2000 2 10 2000 30 10 2000 The Biochemical Society, London © 2001 2001 circular dichroism...
Articles
Coexpression of α and β subunits of prolyl 4-hydroxylase stabilizes the triple helix of recombinant human type X collagen
Available to PurchaseKlaus WAGNER, Ernst PÖSCHL, Javier TURNAY, Jeong-mi BAIK, Taina PIHLAJANIEMI, Svenja FRISCHHOLZ, Klaus VON DER MARK
Journal:
Biochemical Journal
Biochem J (2000) 352 (3): 907–911.
Published: 08 December 2000
..., London © 2000 2000 circular dichroism hydroxylation hydroxyproline thermostability Biochem. J. (2000) 352, 907 911 (Printed in Great Britain) 907 Coexpression of a and b subunits of prolyl 4-hydroxylase stabilizes the triple helix of recombinant human type X collagen Klaus WAGNER*, Ernst PO...
Articles
Human T-lymphotrophic virus type I nucleocapsid protein NCp15: structural study and stability of the N-terminal zinc-finger
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2000) 352 (2): 293–300.
Published: 24 November 2000
... residue rather than to the Ne atom. Two-dimensional NMR techniques were used to determine the solution structure of the zinc-finger, illustrated by the existence of turns in the overall conformation. Key words: circular dichroism, mass spectrometry, peptide synthesis, solution structure, ultraviolet...
Articles
Structure–function studies on Hsp47: pH-dependent inhibition of collagen fibril formation in vitro
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2000) 349 (3): 877–883.
Published: 25 July 2000
... to be the basis of the decreased binding of Hsp47 to collagen in itro at pH 6.3 as well as its inability to inhibit collagen fibril formation at this pH. Our results also provide a functional assay for Hsp47 that can be used in studies on collagen and Hsp47 interactions. Key words: circular dichroism, collagen...
Articles
Membrane-induced conformational change in human apolipoprotein H
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2000) 348 (1): 103–106.
Published: 09 May 2000
... is correlated with a certain conformational change in the secondary structure of the protein. 1 To whom correspondence should be addressed (e-mail suisf@;mail.tsinghua.edu.cn). 7 10 1999 7 2 2000 29 2 2000 The Biochemical Society, London © 2000 2000 circular dichroism 2...
Articles
Interaction of the N-terminus of sterol carrier protein 2 with membranes: role of membrane curvature
Available to Purchase
Journal:
Biochemical Journal
Biochem J (1999) 344 (2): 593–603.
Published: 24 November 1999
... curvature as well as on lipid composition. 1 To whom correspondence should be addressed (e-mail [email protected]). 24 5 1999 1 9 1999 22 9 1999 The Biochemical Society, London © 1999 1999 amphipathic peptide cholesterol circular dichroism α-helix lipid transfer...
Articles
Lipid-binding and antimicrobial properties of synthetic peptides of bovine apolipoprotein A-II
Available to PurchaseMitsuyoshi MOTIZUKI, Takehito ITOH, Takanori SATOH, Sadaki YOKOTA, Muneo YAMADA, Seiichi SHIMAMURA, Tatsuya SAMEJIMA, Kunio TSURUGI
Journal:
Biochemical Journal
Biochem J (1999) 342 (1): 215–221.
Published: 10 August 1999
... correspondence should be addressed (e-mail [email protected]). 7 1 1999 19 4 1999 4 6 1999 The Biochemical Society, London © 1999 1999 Antimicrobial peptide apolipoprotein A-II amphipathic helix circular dichroism Biochem. J. (1999) 342, 215 221 (Printed...
Articles
Structural characterization of DNA and RNA sequences recognized by the gene 5 protein of bacteriophage fd
Available to Purchase
Journal:
Biochemical Journal
Biochem J (1999) 339 (3): 525–531.
Published: 26 April 1999
... Society, London © 1999 1999 circular dichroism dimethyl sulphate footprinting guanine quartets single-stranded-DNA-binding proteins tetraplexes Biochem. J. (1999) 339, 525 531 (Printed in Great Britain) 525 Structural characterization of DNA and RNA sequences recognized by the gene 5 protein...
Articles
Prediction and experimental testing of Bacillus acidocaldarius thioredoxin stability
Available to Purchase
Journal:
Biochemical Journal
Biochem J (1999) 339 (2): 309–317.
Published: 08 April 1999
...-directed mutagenesis. circular dichroism molecular dynamics protein engineering thermostabil Biochem. J. (1999) 339, 309 317 (Printed in Great Britain) 309 Prediction and experimental testing of Bacillus acidocaldarius thioredoxin stability1 Emilia PEDONE*, Raffaele CANNIO, Michele SAVIANO, Mose...