...-like toxin, and a two-domain antitoxin. In Streptococcus agalactiae , the antitoxin AbiEi negatively autoregulates abiE expression through positively co-operative binding to inverted repeats within the promoter. The human pathogen Mycobacterium tuberculosis encodes four DUF1814 putative toxins, two...

... by nucleotide binding. Although such regulatory domains are found in important enzymes and transporters, the underlying regulatory mechanism has only begun to come into focus. We reported previously that CBS domains bind nucleotides co-operatively and induce positive kinetic co-operativity (non-Michaelian...

... results provide for the first time the experimental evidence of co-operative behaviour in class 1A DHODH regulated by DHO binding and reveal that the initial reductive flavin half-reaction follows a mechanism with two steps. The first step is consistent with FMN reduction and shows a hyperbolic dependence...

... [email protected] or [email protected] ). co-operativity crystal structure DNA-binding protein DNA repair Mycobacterium tuberculosis O 6 -methylguanine-DNA methyltransferase 24 7 2015 19 10 2015 28 10 2015 28 10 2015 © 2016 Authors; published by Portland...

... architecture. At different binding sites, effectors control enzyme activity by inducing allosteric changes at the dimer interface influencing binding of fructose-6-phosphate (F6P). allostery cancer co-operativity glycolysis kinase Tarui's disease 6-Phosphofructokinase (Pfk; EC 2.7.1.11...

... (tryptophan fluorescence) of human WT (wild-type) and GKRP-P446L (a mutation associated with high serum triacylglycerol) in the presence of non-fluorescent GK with its tryptophan residues mutated. Titration of GKRP-WT by GK resulted in a sigmoidal increase in TF, suggesting co-operative PPIs (protein–protein...

.... 13 7 2012 5 11 2012 19 11 2012 19 11 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 co-operativity hexanoyl-CoA Mycobacterium tuberculosis short-chain dehydrogenase/reductase β-oxoacyl reductase Type II fatty acid metabolism...

... transporters able to bind ligands with different stoichiometry, selectivity and co-operativity. The molecular determinants and energetics of interaction are the observables that connect the microscopic to the macroscopic frameworks. The present paper addresses the study and proposes a mechanism for the multi...

... that the protonation of residues Lys 215 and Tyr 224 exhibit co-operativity that is important for MgATP 2− and MgPP i 2− binding/dissociation, and suggest these residues function in electrostatic, but not in general acid, catalysis. 1 To whom correspondence should be sent. Present address: The Genomics Research...

..., which form a discrete domain so that most family members have two, four or six EF-hands. This pairing also enables communication, and many EF-hands display positive co-operativity, thereby minimizing the Ca 2+ signal required to reach protein saturation. The conformational effects of Ca 2+ binding...

... and at moderate ionic strength, in addition to positive co-operativity ( h =2.0–2.4) at pH 7.8 and 6.8, and at pH 9.8 in the presence of salt. Furthermore, NADH binding is positively co-operative below 20 °C ( h ∼3) and negatively co-operative at 40–50 °C ( h ∼0.7), as determined at moderate ionic strength and pH...

..., Escherichia coli -based expression system. Effects of the mutations on kinetic properties, including negative co-operative binding of γ-glutamyl substrate, were evaluated. The mutation P314L did not have any major effect on these parameters and was classified as a neutral mutation. The remaining mutations...

... forms of ACCase were characterized by herbicide-binding co-operativity, whereas, in contrast, sensitive forms of the enzymes were not. Our studies on isolated individual isoforms of ACCase from grasses support and extend previous indications that herbicide binding co-operativity is the only kinetic...

.... CBM28 bound to cellulose having a significant non-crystalline content with an association constant similar to that for its binding to soluble glucans. CBM17 (CBM family 17) and CBM28 modules naturally occur as tandems. The CBM17/CBM28 tandem from Cel5A bound with apparent co-operativity to barley β...

... 9 2000 2 1 2001 21 2 2001 The Biochemical Society, London © 2001 2001 allosteric enzymes co-operativity glucokinase metabolic control regulatory protein Biochem. J. (2001) 355, 787 793 (Printed in Great Britain) 787 Occurrence of paradoxical or sustained control...

.... 2 To whom correspondence should be addressed (e-mail [email protected] ). 10 3 2000 30 5 2000 28 6 2000 The Biochemical Society, London © 2000 2000 contractility isometric force Ca2+-regulation regulatory proteins co-operativity Biochem. J. (2000) 350, 693 699...

... lower than 1 mM. The results suggest that all the EF-hands can contribute co-operatively to calpain activation, but that EF-hand 3, in both subunits, has the highest intrinsic affinity for Ca 2+ and provides the major driving force for conformational change. 1 Present address: MRC Protein...

... and hsp70 with an affinity in the range of 10 5 M -1 . Dynamics of hsp90-p60 complex formation is modulated by ATP through changes in the co-operativity of interaction. At low protein concentrations ATP stabilizes the complex. Binding of p23 to hsp90 did not change the affinity of the hsp90-p60 complex...

...-mail [email protected]). 23 6 1999 14 10 1999 5 11 1999 The Biochemical Society, London © 2000 2000 co-operativity Hehre mechanism transglycosylation Biochem. J. (2000) 345, 315 319 (Printed in Great Britain) 315 Hydrolyses of a- and b-cellobiosyl fluorides by Cel6A...

... the regulatory mechanism of the reversible phosphorylation reaction toward certain enzymes is being assessed. enzyme kinetics conformational change co-operativity inactivation rate equation Biochem. J. (1999) 341, 545 554 (Printed in Great Britain) 545 Influence of substrates on in vitro...