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Keywords: conformational change
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Biochem J (2017) 474 (12): 1993–2007.
Published: 30 May 2017
...) elicits major conformational changes of the transporters resulting in the transport of the substrate across the membrane. The availability of a crystal structure of the NBDs enabled us to elucidate the local structure and small-scale dynamics in the NBDs. Here, we labeled the ABC transporter MsbA...
Includes: Supplementary data
Biochem J (2015) 472 (3): 339–352.
Published: 27 November 2015
... 9 2015 6 10 2015 6 10 2015 conformational change recombinant protein expression small-angle X-ray scattering (SAXS) Mass spectrometry analysis reveals that the three essential subunits of the cadherin–catenin complex are phosphorylated in vivo . Examination...
Biochem J (2015) 468 (3): 475–484.
Published: 15 June 2015
.... Lastly, we explore the conformational changes that occur in class I KARIs upon binding of cofactor and metal ions. The class I KARI structures indicate that the active sites close upon binding NAD(P)H, similar to what is observed in the class II KARIs of rice and spinach and different from the opening...
Includes: Supplementary data
Biochem J (2015) 466 (3): 625–637.
Published: 06 March 2015
... functions during ESCRT-mediated membrane reorganization events. conformational change membrane curvature molecular modelling multivesicular body small angle X-ray scattering (SAXS) Active cell-surface receptors and other transmembrane proteins undergo down-regulation through the generation...
Biochem J (2011) 439 (2): 215–225.
Published: 28 September 2011
... insights into the catalytic mechanism of hGSTk and other GSTks. Substrate binding induces a conformational change of the active site from an ‘open’ conformation in the apo-form to a ‘closed’ conformation in the GTX-bound complex, facilitating formations of the G site (GSH-binding site) and the H site...
Biochem J (2010) 429 (2): 379–389.
Published: 28 June 2010
... change dermatan sulfate heparin non-essential activation substrate inhibition Allostery is the coupling of conformational changes between two separated sites. Initially described in oligomeric systems [ 1 – 3 ], allostery is known today as a widely used mechanism in the regulation...
Includes: Supplementary data
Biochem J (2009) 417 (2): 477–483.
Published: 23 December 2008
.... This minidomain bound to Zn 2+ with a stoichiometry of ≈0.7 and an apparent dissociation constant of <500 nM. Fluorescence quenching and 8-anilinonaphthalene-1-sulfonic acid fluorescence methods revealed that Zn 2+ binding resulted in conformational changes characterized by shielding of hydrophobic regions...
Includes: Supplementary data
Biochem J (2007) 401 (1): 353–363.
Published: 11 December 2006
... and in neuroendocrine cells. K d was determined to be 1.2×10 −7 M in the presence of Ca 2+ and 1.5×10 −6 M in the absence of Ca 2+ . The comparatively low Ca 2+ affinity for secretagogin and the fact that it undergoes Ca 2+ -induced conformational changes and interacts with SNAP-25 raise the possibility...
Includes: Supplementary data
Biochem J (2006) 396 (1): 17–21.
Published: 26 April 2006
... including apparent masking of changes in Δ H ‡ by an endothermic conformational change suggests a difference in mechanism involving kinetically significant conformational change. 1 Present address: Assay Development, GlaxoSmithKline Research & Development Ltd, New Frontiers Science Park (North...
Biochem J (2005) 392 (3): 443–448.
Published: 06 December 2005
... occupancy at both substrate-binding sites (Hcy plus betaine, or Hcy plus DMG). Betaine or DMG alone do not bind to the enzyme, indicating that the conformational change associated with Hcy binding creates the betaine-binding site. CBHcy [ S -(δ-carboxybutyl)- D , L -homocysteine] is a bisubstrate analogue...
Biochem J (2005) 391 (2): 203–213.
Published: 10 October 2005
... activation of gene expression is extremely rapid and change in temperature affects the DNA-binding properties of H-NS. The reduction in gene repression brought about by the increase in temperature is concomitant with a conformational change in the protein, resulting in the decrease in size of high-order...
Includes: Supplementary data
Biochem J (2005) 387 (3): 695–702.
Published: 26 April 2005
...Bill X. HUANG; Chhabil DASS; Hee-Yong KIM Mass spectrometry with chemical cross-linking was used to probe the conformational changes of HSA (human serum albumin) in solution on interaction with monounsaturated OA (oleic acid) or polyunsaturated AA (arachidonic acid) or DHA (docosahexaenoic acid...
Biochem J (2004) 380 (2): 401–407.
Published: 01 June 2004
..., the precise conformational changes that take place in head region of integrins (the site of ligand binding) during activation are not well understood. The portion of the integrin β subunit involved in ligand recognition contains a von Willebrand factor type A domain, which comprises a central β-sheet...
Biochem J (2004) 380 (1): 273–282.
Published: 15 May 2004
...Edmond WONG; Alana M. THACKRAY; Raymond BUJDOSO Prion diseases are characterized by conformational change in the copper-binding protein PrP (prion protein). Polymorphisms in ovine PrP at amino acid residues 136, 154 and 171 are associated with variation in susceptibility to scrapie. PrP VRQ [PrP...
Biochem J (2003) 372 (1): 33–40.
Published: 15 May 2003
... occurring in protein interaction. We have developed a novel approach to monitoring conformational changes of proteins in intact cells. A double-labelled fluorescent green fluorescent protein–yellow fluorescent protein (GFP–YFP) fusion protein has been constructed, allowing the exploitation of enhanced...
Biochem J (2003) 372 (1): e1–e2.
Published: 15 May 2003
...Robert INSALL This commentary describes the study by Calleja and co-workers, who have used fluorescence resonance energy transfer between two fluorescent proteins fused to one protein kinase (protein kinase B/Akt) to reveal conformational changes in a functional protein. The findings of their study...
Biochem J (2003) 369 (1): 39–44.
Published: 01 January 2003
... To whom correspondence should be addressed (e-mail: cambillau@afmb.cnrs-mrs.fr and darbon@afmb.cnrs-mrs.fr ). 2 8 2002 9 9 2002 9 9 2002 The Biochemical Society, London ©2003 2003 conformational change membrane NMR pheromone-binding protein Abbreviations used...
Biochem J (2001) 358 (3): 783–790.
Published: 10 September 2001
... phox is supported by the finding that p47 phox undergoes better phosphorylation by CK2 in the presence of arachidonic acid, a known activator of NADPH oxidase which induces conformational changes in p47 phox . In addition, 5,6-dichloro-1-β- o -ribofuranosyl benzimidazole, a CK2 inhibitor, potentiates...
Biochem J (2001) 358 (2): 281–285.
Published: 24 August 2001
...-kinase activation induced a stable change in PKC∊, which predisposed it to subsequent activation by lipid cofactors. Indeed, partial proteolysis of PKC∊ indicated that early activation of PI 3-kinase led to a conformation change in PKC∊ that persisted as the activity of PKC∊ cycled. We propose a two-step...
Biochem J (2000) 349 (3): 835–841.
Published: 25 July 2000
...-sulphonic acid as a probe showed that low pH may induce the conformational change of TCS that leads to the hydrophobic-site exposure, and the CD result showed that this conformational change did not alter its secondary structure. Such conformational change leads to an intermediate state, called the ‘molten...
Biochem J (1999) 341 (3): 545–554.
Published: 26 July 1999
... the regulatory mechanism of the reversible phosphorylation reaction toward certain enzymes is being assessed. enzyme kinetics conformational change co-operativity inactivation rate equation Biochem. J. (1999) 341, 545 554 (Printed in Great Britain) 545 Influence of substrates on in vitro...