... appeared after 1 day and did not change appearance after 4 days. Data collection and analysis was performed as described above. Model and structure factors have been deposited to the PDB with the ID 6FDZ (crystallographic parameters in Supplementary Table S1). conformational changes enzyme...

... or [email protected] ). 13 4 2016 25 4 2016 19 5 2016 19 5 2016 © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society 2016 coagulation Factor IX conformational changes crystal structure enzyme kinetics substrate-binding site...

... transiently associate to form a tetramer that acts as an allosteric switch for higher rate of GMP formation. Biochemical and biophysical studies reveal that sequential conformational changes and interdomain communications regulate tetramer formation via dimer. Our studies also show that overexpression...

... biochemical data are inconsistent with a known high-resolution crystal structure of the catalytic intermediate of the protein, and biophysical data indicate that the protein undergoes conformational changes that affect enzyme activity. In order to further elucidate the solution structure of this important...

..., this equilibrium depends on the presence or absence of nucleotides. To gain further insights into the underlying conformational changes, we applied FRET techniques of single cysteine mutants of OpuAA in solution. Since the crystal structures of MalK from E. coli are available in the nucleotide-free and ATP...

... in affinity for binding of warfarin to H242Q and K199A and a smaller decrease in affinity for W214A and R218H. The findings show that Trp-214 is not as essential for the high-affinity binding ofwarfarin as has previously been thought. Key words: conformational changes, high-affinity binding, protein stability...