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Articles

Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana

Snežana Vojvodić, Marina Stanić, Bernd Zechmann, Tanja Dučić, Milan Žižić, Milena Dimitrijević, Jelena Danilović Luković, Milica R. Milenković, Jon K. Pittman, Ivan Spasojević

Biochem J (2020) 477 (19): 3729–3741.

https://doi.org/10.1042/BCJ20200600

Published: 05 October 2020

...Snežana Vojvodić; Marina Stanić; Bernd Zechmann; Tanja Dučić; Milan Žižić; Milena Dimitrijević; Jelena Danilović Luković; Milica R. Milenković; Jon K. Pittman; Ivan Spasojević Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal...

View articletitled, Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana

Articles

Response to the letter ‘Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus ’

Yong-Sung Park, Suzie Kang, Hyewon Seo, Cheol-Won Yun

Biochem J (2020) 477 (16): 3055–3058.

https://doi.org/10.1042/BCJ20200341

Published: 26 August 2020

...Yong-Sung Park; Suzie Kang; Hyewon Seo; Cheol-Won Yun The answer to the letter ‘Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus ’ has been prepared. We explained our data and showed supplementary information to answer the questions. And we respect the results...

View articletitled, Response to the letter ‘Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus ’

Includes: Supplementary data

Articles

Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus

Annie Yap, Matthias Misslinger, Hubertus Haas

Biochem J (2020) 477 (16): 2967–2970.

https://doi.org/10.1042/BCJ20200286

Published: 19 August 2020

...Annie Yap; Matthias Misslinger; Hubertus Haas Aspergillus fumigatus is the most common cause of invasive aspergillosis, a life-threatening infection mainly affecting immunocompromised patients. The essential metals copper and iron play crucial roles in virulence of this mold. Recently, the copper...

View articletitled, Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus

Articles

Copper-dependent ATP7B up-regulation drives the resistance of TMEM16A-overexpressing head-and-neck cancer models to platinum toxicity

Avani Vyas, Umamaheswar Duvvuri, Kirill Kiselyov

Biochem J (2019) 476 (24): 3705–3719.

https://doi.org/10.1042/BCJ20190591

Published: 17 December 2019

... (NOX2), by the antioxidant N-Acetyl-Cysteine (NAC) and by copper chelation using cuprizone and bathocuproine sulphonate (BCS). Pretreatment with either chelator significantly increased cisplatin's sensitivity, particularly in the context of TMEM16A overexpression. We propose that increased oxidative...

View articletitled, Copper-dependent ATP7B up-regulation drives the resistance of TMEM16A-overexpressing head-and-neck cancer models to platinum toxicity

Articles

A copper transcription factor, AfMac1, regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus

Yong-Sung Park, Suzie Kang, Hyewon Seo, Cheol-Won Yun

Biochem J (2018) 475 (17): 2831–2845.

https://doi.org/10.1042/BCJ20180399

Published: 11 September 2018

...Yong-Sung Park; Suzie Kang; Hyewon Seo; Cheol-Won Yun Although iron and copper are co-ordinately regulated in living cells, the homeostatic effects of each of these metals on the other remain unknown. Here, we show the function of AfMac1, a transcriptional activator of the copper and iron regulons...

View articletitled, A copper transcription factor, AfMac1, regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus

Includes: Supplementary data

Articles

Functional characterization of the copper transcription factor AfMac1 from Aspergillus fumigatus

Yong-Sung Park, Tae-Hyoung Kim, Cheol-Won Yun

Biochem J (2017) 474 (14): 2365–2378.

https://doi.org/10.1042/BCJ20170191

Published: 03 July 2017

...Yong-Sung Park; Tae-Hyoung Kim; Cheol-Won Yun Although copper functions as a cofactor in many physiological processes, copper overload leads to harmful effects in living cells. Thus, copper homeostasis is tightly regulated. However, detailed copper metabolic pathways have not yet been identified...

View articletitled, Functional characterization of the copper transcription factor AfMac1 from Aspergillus fumigatus

Articles

Transition metals activate TFEB in overexpressing cells

Karina A. Peña, Kirill Kiselyov

Biochem J (2015) 470 (1): 65–76.

https://doi.org/10.1042/BJ20140645

Published: 06 August 2015

... to drive gene expression in response to transition metal exposure and that such activation may influence transition metal toxicity. We found that transition metals copper (Cu) and iron (Fe) activate recombinant TFEB and stimulate the expression of TFEB-dependent genes in TFEB-overexpressing cells. In cells...

View articletitled, Transition metals activate TFEB in overexpressing cells

Articles

Cu 2+ accentuates distinct misfolding of Aβ (1–40) and Aβ (1–42) peptides, and potentiates membrane disruption

Christian J. Matheou, Nadine D. Younan, John H. Viles

Biochem J (2015) 466 (2): 233–242.

https://doi.org/10.1042/BJ20141168

Published: 20 February 2015

...+ accentuate differences in the propensity of Aβ (1–40) and Aβ (1–42) to form synaptotoxic oligomers, providing an explanation for the connection between disrupted Cu 2+ homoeostasis and elevated Aβ (1–42) neurotoxicity in Alzheimer's disease. Alzheimer’s disease amyloid copper oligomers lipid-membrane...

View articletitled, Cu 2+ accentuates distinct misfolding of Aβ (1–40) and Aβ (1–42) peptides, and potentiates membrane disruption

Articles

Serum copper as a novel biomarker for resistance to thyroid hormone

Jens Mittag, Thomas Behrends, Kristina Nordström, Joao Anselmo, Björn Vennström, Lutz Schomburg

Biochem J (2012) 443 (1): 103–109.

https://doi.org/10.1042/BJ20111817

Published: 14 March 2012

...-secreting pituitary adenomas, which present with clinically similar symptoms. In the present paper we show that serum copper levels are regulated by thyroid hormone, which stimulates the synthesis and the export of the hepatic copper-transport protein ceruloplasmin into the serum. This is accompanied...

View articletitled, Serum copper as a novel biomarker for resistance to thyroid hormone

Includes: Supplementary data

Articles

Clioquinol induces cytoplasmic clearance of the X-linked inhibitor of apoptosis protein (XIAP): therapeutic indication for prostate cancer

Michael A. Cater, Ygal Haupt

Biochem J (2011) 436 (2): 481–491.

https://doi.org/10.1042/BJ20110123

Published: 13 May 2011

...Michael A. Cater; Ygal Haupt Clioquinol (5-chloro-7-iodo-8-quinolinol) is a copper ionophore that was used primarily during the 1950–1970s as an oral antimicrobial agent. It has been established that clioquinol displays toxicity towards malignant cells, inducing caspase-dependent apoptosis...

View articletitled, Clioquinol induces cytoplasmic clearance of the X-linked inhibitor of apoptosis protein (XIAP): therapeutic indication for prostate cancer

Includes: Supplementary data

Articles

Cadmium regulates copper homoeostasis by inhibiting the activity of Mac1, a transcriptional activator of the copper regulon, in Saccharomyces cerevisiae

Dong-Hyuk Heo, In-Joon Baek, Hyun-Jun Kang, Ji-Hyun Kim, Miwha Chang, Mi-Young Jeong, Tae-Hyoung Kim, Il-Dong Choi, Cheol-Won Yun

Biochem J (2010) 431 (2): 257–265.

https://doi.org/10.1042/BJ20100638

Published: 28 September 2010

... as a model system to study cadmium toxicity at the molecular level because it has been used to identify the molecular mechanisms of toxicity found in higher organisms. cDNA microarray and Northern blot analyses revealed that cadmium salts inhibited the expression of genes related to copper metabolism...

View articletitled, Cadmium regulates copper homoeostasis by inhibiting the activity of Mac1, a transcriptional activator of the copper regulon, in Saccharomyces cerevisiae

Articles

Characterization of the transition-metal-binding properties of hepcidin

Chris Tselepis, Samuel J. Ford, Andrew T. McKie, Wolfgang Vogel, Heinz Zoller, Robert J. Simpson, Javier Diaz Castro, Tariq H. Iqbal, Douglas G. Ward

Biochem J (2010) 427 (2): 289–296.

https://doi.org/10.1042/BJ20091521

Published: 29 March 2010

... the binding of a range of metal ions to wild-type and mutant human and murine hepcidins. In addition, the biological effects of these point mutations were tested on Caco-2 and HEK-293T human cell lines and in mice. Our results show that hepcidin-25 can form complexes with copper, nickel and zinc; however, we...

View articletitled, Characterization of the transition-metal-binding properties of hepcidin

Articles

Differential contributions of Glu 96 , Asp 102 and Asp 111 to coagulation Factor V/Va metal ion binding and subunit stability

Jina Song, Kimberley Talbot, Jeffrey Hewitt, Ross T. A. MacGillivray, Edward L. G. Pryzdial

Biochem J (2009) 422 (2): 257–264.

https://doi.org/10.1042/BJ20090405

Published: 13 August 2009

... coagulation copper Factor V subunit In the presence of Ca 2+ and an aPL (anionic phospholipid)-containing membrane, coagulation FVa [activated FV (Factor V)] accelerates the activation of prothrombin to thrombin by the serine protease FXa (activated Factor X); this represents the critical step...

View articletitled, Differential contributions of Glu 96 , Asp 102 and Asp 111 to coagulation Factor V/Va metal ion binding and subunit stability

Articles

Intracellular copper deficiency increases amyloid-β secretion by diverse mechanisms

Michael A. Cater, Kelly T. McInnes, Qiao-Xin Li, Irene Volitakis, Sharon La Fontaine, Julian F. B. Mercer, Ashley I. Bush

Biochem J (2008) 412 (1): 141–152.

https://doi.org/10.1042/BJ20080103

Published: 25 April 2008

...Michael A. Cater; Kelly T. McInnes; Qiao-Xin Li; Irene Volitakis; Sharon La Fontaine; Julian F. B. Mercer; Ashley I. Bush In Alzheimer's disease there is abnormal brain copper distribution, with accumulation of copper in amyloid plaques and a deficiency of copper in neighbouring cells. Excess...

View articletitled, Intracellular copper deficiency increases amyloid-β secretion by diverse mechanisms

Articles

The chemistry of copper binding to PrP: is there sufficient evidence to elucidate a role for copper in protein function?

Paul Davies, David R. Brown

Biochem J (2008) 410 (2): 237–244.

https://doi.org/10.1042/BJ20071477

Published: 12 February 2008

...Paul Davies; David R. Brown There has been an enormous body of literature published in the last 10 years concerning copper and PrP (prion protein). Despite this, there is still no generally accepted role for copper in the function of PrP or any real consensus as to how and to what affinity copper...

View articletitled, The chemistry of copper binding to PrP: is there sufficient evidence to elucidate a role for copper in protein function?

Articles

Differential modulation of Alzheimer's disease amyloid β-peptide accumulation by diverse classes of metal ligands

Aphrodite Caragounis, Tai Du, Gulay Filiz, Katrina M. Laughton, Irene Volitakis, Robyn A. Sharples, Robert A. Cherny, Colin L. Masters, Simon C. Drew, Andrew F. Hill, Qiao-Xin Li, Peter J. Crouch, Kevin J. Barnham, Anthony R. White

Biochem J (2007) 407 (3): 435–450.

https://doi.org/10.1042/BJ20070579

Published: 12 October 2007

... 8HQ and phenanthroline derivatives and the sulfur compound PDTC (pyrrolidine dithiocarbamate) elevated cellular metal levels (copper and zinc), and resulted in substantial loss of secreted Aβ. Generally, the ability to inhibit Aβ levels correlated with a higher lipid solubility of the ligands...

View articletitled, Differential modulation of Alzheimer's disease amyloid β-peptide accumulation by diverse classes of metal ligands

Articles

Hormonal regulation of the Menkes and Wilson copper-transporting ATPases in human placental Jeg-3 cells

Belinda Hardman, Agnes Michalczyk, Mark Greenough, James Camakaris, Julian F. B. Mercer, M. Leigh Ackland

Biochem J (2007) 402 (2): 241–250.

https://doi.org/10.1042/BJ20061099

Published: 12 February 2007

...Belinda Hardman; Agnes Michalczyk; Mark Greenough; James Camakaris; Julian F. B. Mercer; M. Leigh Ackland Copper deficiency during pregnancy results in early embryonic death and foetal structural abnormalities including skeletal, pulmonary and cardiovascular defects. During pregnancy, copper...

View articletitled, Hormonal regulation of the Menkes and Wilson copper-transporting ATPases in human placental Jeg-3 cells

Articles

Regulation of ceruloplasmin in human hepatic cells by redox active copper: identification of a novel AP-1 site in the ceruloplasmin gene

Dola Das, Nisha Tapryal, Shyamal K. Goswami, Paul L. Fox, Chinmay K. Mukhopadhyay

Biochem J (2007) 402 (1): 135–141.

https://doi.org/10.1042/BJ20060963

Published: 25 January 2007

...Dola Das; Nisha Tapryal; Shyamal K. Goswami; Paul L. Fox; Chinmay K. Mukhopadhyay Cp (ceruloplasmin), a copper containing plasma protein, mainly synthesized in the liver, is known to be functional between the interface of iron and copper metabolism. We have reported previously that Cp is regulated...

View articletitled, Regulation of ceruloplasmin in human hepatic cells by redox active copper: identification of a novel AP-1 site in the ceruloplasmin gene

Articles

Characterization of COMMD protein–protein interactions in NF-κB signalling

Prim de Bie, Bart van de Sluis, Ezra Burstein, Karen J. Duran, Ruud Berger, Colin S. Duckett, Cisca Wijmenga, Leo W. J. Klomp

Biochem J (2006) 398 (1): 63–71.

https://doi.org/10.1042/BJ20051664

Published: 27 July 2006

...Prim de Bie; Bart van de Sluis; Ezra Burstein; Karen J. Duran; Ruud Berger; Colin S. Duckett; Cisca Wijmenga; Leo W. J. Klomp COMMD [copper metabolism gene MURR1 (mouse U2af1-rs1 region 1) domain] proteins constitute a recently identified family of NF-κB (nuclear factor κB)-inhibiting proteins...

View articletitled, Characterization of COMMD protein–protein interactions in NF-κB signalling

Articles

Functional expression of a low-affinity zinc uptake transporter ( Fr ZIP2) from pufferfish ( Takifugu rubripes ) in MDCK cells

Andong Qiu, Christer Hogstrand

Biochem J (2005) 390 (3): 777–786.

https://doi.org/10.1042/BJ20050568

Published: 05 September 2005

... ). The nucleotide sequence data reported will appear in DDBJ, EMBL, GenBank® and GSDB Nucleotide Sequence Databases under the accession number AY529486. 6 4 2005 19 5 2005 20 5 2005 20 5 2005 The Biochemical Society, London 2005 copper influx MDCK cell pufferfish SLC39A3...

View articletitled, Functional expression of a low-affinity zinc uptake transporter ( Fr ZIP2) from pufferfish ( Takifugu rubripes ) in MDCK cells

Articles

Separation of native prion protein (PrP) glycoforms by copper-binding using immobilized metal affinity chromatography (IMAC)

Henrik MÜLLER, Alexander STROM, Gerhard HUNSMANN, Andreas W. STUKE

Biochem J (2005) 388 (1): 371–378.

https://doi.org/10.1042/BJ20041291

Published: 10 May 2005

...-linked glycosylation sites and binds copper under physiological conditions. In contrast with PrP C , PrP Sc is insoluble in non-ionic detergents and does not bind to Cu 2+ ions. Hence, we utilized copper binding to separate and characterize both PrP isoforms. Infected and uninfected murine brain...

View articletitled, Separation of native prion protein (PrP) glycoforms by copper-binding using immobilized metal affinity chromatography (IMAC)

Articles

Fragmentation and dimerization of copper-loaded prion protein by copper-catalysed oxidation

Noriyuki SHIRAISHI, Yoko INAI, Wenxiang BI, Morimitsu NISHIKIMI

Biochem J (2005) 387 (1): 247–255.

https://doi.org/10.1042/BJ20041561

Published: 22 March 2005

... that the N-terminal domain binds five Cu 2+ ions. In the present study, we have investigated copper-catalysed oxidation of a recombinant mouse prion protein, PrP 23–231 . The copper-loaded PrP 23–231 was found to be carbonylated by incubation with dopamine. Besides the formation of carbonyls, a cross-linked...

View articletitled, Fragmentation and dimerization of copper-loaded prion protein by copper-catalysed oxidation

Articles

Copper homoeostasis in Drosophila melanogaster S2 cells

Adam SOUTHON, Richard BURKE, Melanie NORGATE, Philip BATTERHAM, James CAMAKARIS

Biochem J (2004) 383 (2): 303–309.

https://doi.org/10.1042/BJ20040745

Published: 08 October 2004

...Adam SOUTHON; Richard BURKE; Melanie NORGATE; Philip BATTERHAM; James CAMAKARIS Copper homoeostasis was investigated in the Drosophila melanogaster S2 cell line to develop an insect model for the study of copper regulation. Real-time PCR studies have demonstrated expression in S2 cells of putative...

View articletitled, Copper homoeostasis in Drosophila melanogaster S2 cells

Articles

Copper pathways in Plasmodium falciparum infected erythrocytes indicate an efflux role for the copper P-ATPase

Dominique RASOLOSON, Lirong SHI, Curtis R. CHONG, Bjorn F. KAFSACK, David J. SULLIVAN, Jr

Biochem J (2004) 381 (3): 803–811.

https://doi.org/10.1042/BJ20040335

Published: 27 July 2004

...Dominique RASOLOSON; Lirong SHI; Curtis R. CHONG; Bjorn F. KAFSACK; David J. SULLIVAN, Jr Copper, like iron, is a transition metal that can generate oxygen radicals by the Fenton reaction. The Plasmodium parasite invades an erythrocyte host cell containing 20 μM copper, of which 70% is contained...

View articletitled, Copper pathways in Plasmodium falciparum infected erythrocytes indicate an efflux role for the copper P-ATPase

Articles

Intracellular trafficking of the human Wilson protein: the role of the six N-terminal metal-binding sites

Michael A. CATER, John FORBES, Sharon La FONTAINE, Diane COX, Julian F. B. MERCER

Biochem J (2004) 380 (3): 805–813.

https://doi.org/10.1042/bj20031804

Published: 15 June 2004

...Michael A. CATER; John FORBES; Sharon La FONTAINE; Diane COX; Julian F. B. MERCER The Wilson protein (ATP7B) is a copper-transporting CPx-type ATPase defective in the copper toxicity disorder Wilson disease. In hepatocytes, ATP7B delivers copper to apo-ceruloplasmin and mediates the excretion...

View articletitled, Intracellular trafficking of the human Wilson protein: the role of the six N-terminal metal-binding sites

Articles

Copper induces increased beta-sheet content in the scrapie-susceptible ovine prion protein PrPVRQ compared with the resistant allelic variant PrPARR

Edmond WONG, Alana M. THACKRAY, Raymond BUJDOSO

Biochem J (2004) 380 (1): 273–282.

https://doi.org/10.1042/bj20031767

Published: 15 May 2004

...Edmond WONG; Alana M. THACKRAY; Raymond BUJDOSO Prion diseases are characterized by conformational change in the copper-binding protein PrP (prion protein). Polymorphisms in ovine PrP at amino acid residues 136, 154 and 171 are associated with variation in susceptibility to scrapie. PrP VRQ [PrP...

View articletitled, Copper induces increased beta-sheet content in the scrapie-susceptible ovine prion protein PrPVRQ compared with the resistant allelic variant PrPARR

Articles

Copper stimulates trafficking of a distinct pool of the Menkes copper ATPase (ATP7A) to the plasma membrane and diverts it into a rapid recycling pool

Luke PASE, Ilia VOSKOBOINIK, Mark GREENOUGH, James CAMAKARIS

Biochem J (2004) 378 (3): 1031–1037.

https://doi.org/10.1042/bj20031181

Published: 15 March 2004

...Luke PASE; Ilia VOSKOBOINIK; Mark GREENOUGH; James CAMAKARIS MNK (Menkes copper-translocating P-type ATPase, or the Menkes protein; ATP7A) plays a key role in regulating copper homoeostasis in humans. MNK has been shown to have a dual role in the cell: it delivers copper to cuproenzymes...

View articletitled, Copper stimulates trafficking of a distinct pool of the Menkes copper ATPase (ATP7A) to the plasma membrane and diverts it into a rapid recycling pool

Articles

Metallothionein is crucial for safe intracellular copper storage and cell survival at normal and supra-physiological exposure levels

Lucía TAPIA, Mauricio GONZÁLEZ-AGÜERO, Mónica F. CISTERNAS, Miriam SUAZO, Verónica CAMBIAZO, Ricardo UAUY, Mauricio GONZÁLEZ

Biochem J (2004) 378 (2): 617–624.

https://doi.org/10.1042/bj20031174

Published: 01 March 2004

...Lucía TAPIA; Mauricio GONZÁLEZ-AGÜERO; Mónica F. CISTERNAS; Miriam SUAZO; Verónica CAMBIAZO; Ricardo UAUY; Mauricio GONZÁLEZ MTs (metallothioneins) increase the resistance of cells to exposure to high Cu (copper) levels. Characterization of the MT–Cu complex suggests that MT has an important role...

View articletitled, Metallothionein is crucial for safe intracellular copper storage and cell survival at normal and supra-physiological exposure levels

Articles

The nucleotide-binding domain of the Zn2+-transporting P-type ATPase from Escherichia coli carries a glycine motif that may be involved in binding of ATP

Juha OKKERI, Liisa LAAKKONEN, Tuomas HALTIA

Biochem J (2004) 377 (1): 95–105.

https://doi.org/10.1042/bj20030740

Published: 01 January 2004

... be addressed (e-mail [email protected] or [email protected] ). 20 5 2003 5 9 2003 26 9 2003 26 9 2003 The Biochemical Society, London ©2004 2004 copper metal ion transport nucleotide-binding domain P 1 -type ATPase Wilson disease Abbreviations...

View articletitled, The nucleotide-binding domain of the Zn2+-transporting P-type ATPase from Escherichia coli carries a glycine motif that may be involved in binding of ATP

Articles

Inhibition of oxygen evolution in Photosystem II by Cu(II) ions is associated with oxidation of cytochrome b559

Kvetoslava BURDA, Jerzy KRUK, Georg H. SCHMID, Kazimierz STRZALKA

Biochem J (2003) 371 (2): 597–601.

https://doi.org/10.1042/bj20021265

Published: 15 April 2003

...Kvetoslava BURDA; Jerzy KRUK; Georg H. SCHMID; Kazimierz STRZALKA We have found that elevated copper concentrations, apart from the inhibition of oxygen evolution, changed the initial states distribution of the oxygen-evolving complex. Already at low concentrations, copper ions oxidized the low...

View articletitled, Inhibition of oxygen evolution in Photosystem II by Cu(II) ions is associated with oxidation of cytochrome b559

Articles

Probing the catalytic mechanism of Escherichia coli amine oxidase using mutational variants and a reversible inhibitor as a substrate analogue

Colin G. SAYSELL, Winston S. TAMBYRAJAH, Jeremy M. MURRAY, Carrie M. WILMOT, Simon E.V. PHILLIPS, Michael J. McPHERSON, Peter F. KNOWLES

Biochem J (2002) 365 (3): 809–816.

https://doi.org/10.1042/bj20011435

Published: 01 August 2002

...Colin G. SAYSELL; Winston S. TAMBYRAJAH; Jeremy M. MURRAY; Carrie M. WILMOT; Simon E.V. PHILLIPS; Michael J. McPHERSON; Peter F. KNOWLES Copper amine oxidases are homodimeric enzymes containing one Cu 2+ ion and one 2,4,5-trihydroxyphenylalanine quinone (TPQ) per monomer. Previous studies...

View articletitled, Probing the catalytic mechanism of Escherichia coli amine oxidase using mutational variants and a reversible inhibitor as a substrate analogue

Articles

Multiple forms of the catalytic centre, CuZ, in the enzyme nitrous oxide reductase from Paracoccus pantotrophus

Tim RASMUSSEN, Ben C. BERKS, Julea N. BUTT, Andrew J. THOMSON

Biochem J (2002) 364 (3): 807–815.

https://doi.org/10.1042/bj20020055

Published: 15 June 2002

...Tim RASMUSSEN; Ben C. BERKS; Julea N. BUTT; Andrew J. THOMSON Nitrous oxide reductase catalyses the reduction of nitrous oxide to dinitrogen at a unique tetranuclear copper site, called Cu Z , which has a central inorganic sulphide ligand. Limited incubation with oxygen during the preparation...

View articletitled, Multiple forms of the catalytic centre, CuZ, in the enzyme nitrous oxide reductase from Paracoccus pantotrophus

Articles

Metal imbalance and compromised antioxidant function are early changes in prion disease

Alana M. THACKRAY, Robert KNIGHT, Stephen J. HASWELL, Raymond BUJDOSO, David R. BROWN

Biochem J (2002) 362 (2): 253–258.

https://doi.org/10.1042/bj3620253

Published: 22 February 2002

...Alana M. THACKRAY; Robert KNIGHT; Stephen J. HASWELL; Raymond BUJDOSO; David R. BROWN The prion protein (PrP) has been shown to bind copper. In the present study we have investigated whether prion disease in a mouse scrapie model resulted in modification of metal concentrations. We found changes...

View articletitled, Metal imbalance and compromised antioxidant function are early changes in prion disease

Articles

Nitric oxide-dependent pro-oxidant and pro-apoptotic effect of metallothioneins in HL-60 cells challenged with cupric nitrilotriacetate

Shang-Xi LIU, Kazuaki KAWAI, Vladimir A. TYURIN, Yulia Y. TYURINA, Grigory G. BORISENKO, James P. FABISIAK, Peter J. QUINN, Bruce R. PITT, Valerian E. KAGAN

Biochem J (2001) 354 (2): 397–406.

https://doi.org/10.1042/bj3540397

Published: 22 February 2001

... and protecting against electrophiles. We report that MT protection against Cu-induced cytotoxicity can be reversed and pro-oxidant and pro-apoptotic effects can be induced in HL-60 cells exposed to NO. We demonstrate that in ZnCl 2 -pretreated HL-60 cells loaded with copper nitrilotriacetate (Cu-NTA), exposure...

View articletitled, Nitric oxide-dependent pro-oxidant and pro-apoptotic effect of metallothioneins in HL-60 cells challenged with cupric nitrilotriacetate

Articles

PrPSc-like prion protein peptide inhibits the function of cellular prion protein

David R. BROWN

Biochem J (2000) 352 (2): 511–518.

https://doi.org/10.1042/bj3520511

Published: 24 November 2000

... interaction of PrP106–126 with the prion protein was found to make cells more susceptible to copper toxicity, inhibited copper uptake into cells and inhibited the superoxide dismutase-like activity of the prion protein. Direct inhibition of prion protein function by PrP Sc may be necessary...

View articletitled, PrPSc-like prion protein peptide inhibits the function of cellular prion protein

Articles

Normal prion protein has an activity like that of superoxide dismutase

David R. BROWN, Boon-Seng WONG, Farida HAFIZ, Christine CLIVE, Stephen J. HASWELL, Ian M. JONES

Biochem J (1999) 344 (1): 1–5.

https://doi.org/10.1042/bj3440001

Published: 08 November 1999

... with recombinant chicken PrP C confirming the evolutionary conserved phenotype suggested by sequence similarity. Acquisition of copper by PrP C during protein folding endowed SOD activity on the protein but the addition of copper following refolding did not. PrP C dependent SOD activity was abolished by deletion...

View articletitled, Normal prion protein has an activity like that of superoxide dismutase

Articles

Protein and gene structure of a blue laccase from Pleurotus ostreatus

Paola GIARDINA, Gianna PALMIERI, Andrea SCALONI, Bianca FONTANELLA, Vincenza FARACO, Giovanna CENNAMO, Giovanni SANNIA

Biochem J (1999) 341 (3): 655–663.

https://doi.org/10.1042/bj3410655

Published: 26 July 1999

...Paola GIARDINA; Gianna PALMIERI; Andrea SCALONI; Bianca FONTANELLA; Vincenza FARACO; Giovanna CENNAMO; Giovanni SANNIA A new laccase isoenzyme (POXA1b, where POX is phenol oxidase), produced by Pleurotus ostreatus in cultures supplemented with copper sulphate, has been purified and fully...

View articletitled, Protein and gene structure of a blue laccase from Pleurotus ostreatus

Articles

Pyrrolidine dithiocarbamate up-regulates the expression of the genes encoding the catalytic and regulatory subunits of γ-glutamylcysteine synthetase and increases intracellular glutathione levels

Angela C. WILD, R. Timothy MULCAHY

Biochem J (1999) 338 (3): 659–665.

https://doi.org/10.1042/bj3380659

Published: 08 March 1999

... PDTC exposure to increases in the expression of the GCS catalytic and regulatory subunit genes demonstrated that induction by PDTC could be partially blocked by treatment with the thiol agent N -acetylcysteine and by the copper chelator bathocuproine disulphonic acid. These findings suggested...

View articletitled, Pyrrolidine dithiocarbamate up-regulates the expression of the genes encoding the catalytic and regulatory subunits of γ-glutamylcysteine synthetase and increases intracellular glutathione levels

Articles

Identification and characterization of a recombinant metallothionein protein from a marine alga, Fucus vesiculosus

Ceri A. MORRIS, Beate NICOLAUS, Viola SAMPSON, John L. HARWOOD, Peter KILLE

Biochem J (1999) 338 (2): 553–560.

https://doi.org/10.1042/bj3380553

Published: 22 February 1999

...Ceri A. MORRIS; Beate NICOLAUS; Viola SAMPSON; John L. HARWOOD; Peter KILLE A cDNA library was constructed from macroalgae adapted to prolonged elevated environmental copper levels. To investigate the possible existence of a metallothionein (MT) gene, the library was screened with degenerate probes...

View articletitled, Identification and characterization of a recombinant metallothionein protein from a marine alga, Fucus vesiculosus

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