... analysis was performed, and the crystal structure of each mutant protein within the cycle was determined respectively to interpret the coupling energy. The ΔΔ G o values of Y14F/D99L (Tyr 14 →Phe/Asp 99 →Leu) KSI, 25.5 kJ/mol for catalysis and 28.9 kJ/mol for stability, were smaller than the sums (i.e...

... ultracentrifuge techniques, were similar to that of the wild-type enzyme. Initial velocity experiments were performed to derive the various kinetic parameters, which were used to analyse further the free energy change and the coupling energy (ΔΔ G int ) between any two residues. The dissociation constants for Mn...