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1-12 of 12
Keywords: cryo-electron microscopy
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Awards
Journal:
Biochemical Journal
Biochem J (2021) 478 (24): 4169–4185.
Published: 16 December 2021
... 4.0 (CC BY) . computational biochemistry cryo-electron microscopy imaging techniques structural biology Structure determination of biological macromolecules through single-particle analysis of cryo-EM images has recently reached a milestone by obtaining atomic resolution...
Articles
Pu Qian, Tristan I. Croll, David J.K. Swainsbury, Pablo Castro-Hartmann, Nigel W. Moriarty, Kasim Sader, C. Neil Hunter
Journal:
Biochemical Journal
Biochem J (2021) 478 (17): 3253–3263.
Published: 07 September 2021
...Pu Qian; Tristan I. Croll; David J.K. Swainsbury; Pablo Castro-Hartmann; Nigel W. Moriarty; Kasim Sader; C. Neil Hunter The reaction centre light-harvesting 1 (RC–LH1) complex is the core functional component of bacterial photosynthesis. We determined the cryo-electron microscopy (cryo-EM...
Includes: Supplementary data
Articles
Conserved L464 in p97 D1–D2 linker is critical for p97 cofactor regulated ATPase activity
Available to PurchaseXiaoyi Zhang, Lin Gui, Shan Li, Purbasha Nandi, Rod Carlo Columbres, Daniel E. Wong, Derek R. Moen, Henry J. Lin, Po-Lin Chiu, Tsui-Fen Chou
Journal:
Biochemical Journal
Biochem J (2021) 478 (17): 3185–3204.
Published: 07 September 2021
... by p37 and NU is affected by D1–D2 linker mutations to some extent. anti-caner cryo-electron microscopy enzyme kinetics p97/VCP The N–D1 linker (residues 185–210) and the D1–D2 linker (residues 460–481) connect NTD–D1 and D1–D2 domains, respectively (Figure 1A). This structural...
Includes: Supplementary data
Articles
Clathrin: the molecular shape shifter
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2021) 478 (16): 3099–3123.
Published: 26 August 2021
... and Huntington-interacting protein 1-related protein (HIP1R), both of which are known to bind to actin, clathrin and PI(4,5)P 2 [ 111–115 ]. Correspondence: Corinne J. Smith ([email protected]) clathrin cryo-electron microscopy endocytosis Figure 3. Stages in clathrin...
Articles
Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions
Open AccessIdo Caspy, Mariia Fadeeva, Sebastian Kuhlgert, Anna Borovikova-Sheinker, Daniel Klaiman, Gal Masrati, Friedel Drepper, Nir Ben-Tal, Michael Hippler, Nathan Nelson
Journal:
Biochemical Journal
Biochem J (2021) 478 (12): 2371–2384.
Published: 23 June 2021
... 10.1073/pnas.1621051114 17 Zheng , S.Q. , Palovcak , E. , Armache , J.P. , Verba , K.A. , Cheng , Y. and Agard , D.A. (2017) Motioncor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy . Nat. Methods 14 , 331 – 332 10.1038/nmeth...
Includes: Multimedia, Supplementary data
Articles
Current data processing strategies for cryo-electron tomography and subtomogram averaging
Open AccessIn Collection
Awards
Journal:
Biochemical Journal
Biochem J (2021) 478 (10): 1827–1845.
Published: 18 May 2021
... BY) . Recent developments in cryo-electron microscopy (EM) have enabled the determination of structures of macromolecules at atomic resolution using single-particle analysis (SPA) [ 1 , 2 ]. Obtaining atomic and near-atomic resolution enhances our understanding of the precise mechanisms of protein function...
Articles
The transport mechanism of P4 ATPase lipid flippases
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2020) 477 (19): 3769–3790.
Published: 12 October 2020
... cations. Correspondence: Rosa Laura López-Marqués ( [email protected] ) 2 7 2020 2 9 2020 16 9 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 autoinhibition CDC50 protein cryo-electron microscopy flippases lipid...
Articles
Synergistic role of nucleotides and lipids for the self-assembly of Shs1 septin oligomers
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2020) 477 (14): 2697–2714.
Published: 29 July 2020
... mM MgCl 2 in some cases) and 300 mM NaCl. cryo-electron microscopy membranes nucleotide septin At the plasma membrane, in budding yeast Saccharomyces cerevisiae , septins assemble into a collar-shaped structure at the bud neck during cell division ( Figure 1A ). The collar splits...
Includes: Multimedia, Supplementary data
Articles
Effects of N-glycosylation on hyperpolarization-activated cyclic nucleotide-gated (HCN) channels
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2015) 466 (1): 77–84.
Published: 06 February 2015
...) channels, and some but not all of the four subunits of the HCN channel need to be glycosylated for trafficking to cell membrane. cryo-electron microscopy HCN2 fluorescence ion channel membrane reconstitution membrane trafficking N-linked glycosylation The hyperpolarization-activated...
Articles
A urea channel from Bacillus cereus reveals a novel hexameric structure
Available to PurchaseGerard H. M. Huysmans, Nathan Chan, Jocelyn M. Baldwin, Vincent L. G. Postis, Svetomir B. Tzokov, Sarah E. Deacon, Sylvia Y. M. Yao, James D. Young, Michael J. McPherson, Per A. Bullough, Stephen A. Baldwin
Journal:
Biochemical Journal
Biochem J (2012) 445 (2): 157–166.
Published: 27 June 2012
... of seven TM (transmembrane) helices, with a cytoplasmic C-terminus. In detergent, UAC Bc exists largely as a hexamer, as demonstrated by both cross-linking and size-exclusion chromatography. A 9 Å (1 Å=0.1 nm) resolution projection map obtained by cryo-electron microscopy of two-dimensional crystals shows...
Includes: Supplementary data
Articles
Solution structure of the natively assembled yeast ribosomal stalk determined by small-angle X-ray scattering
Available to PurchasePrzemysław Grela, Michal J. Gajda, Jean-Paul Armache, Roland Beckmann, Dawid Krokowski, Dmitri I. Svergun, Nikodem Grankowski, Marek Tchórzewski
Journal:
Biochemical Journal
Biochem J (2012) 444 (2): 205–209.
Published: 11 May 2012
... © The Authors Journal compilation © 2012 Biochemical Society 2012 cryo-electron microscopy GTPase centre ribosomal P protein ribosomal stalk ribosome small-angle X-ray scattering (SAXS) At all stages of protein synthesis the ribosome requires protein factors, known as tGTPases...
Articles
Localization of PKA phosphorylation site, Ser 2030 , in the three-dimensional structure of cardiac ryanodine receptor
Available to PurchasePeter P. Jones, Xing Meng, Bailong Xiao, Shitian Cai, Jeff Bolstad, Terence Wagenknecht, Zheng Liu, S. R. Wayne Chen
Journal:
Biochemical Journal
Biochem J (2008) 410 (2): 261–270.
Published: 12 February 2008
... T2023-GFP and was subsequently used as a basis for three-dimensional reconstruction. Cryo-electron microscopy and single particle image processing of the purified RyR2(A4860G) T2023-GFP protein revealed the location of the inserted GFP, and hence the Ser 2030 PKA site in domain 4, a region that may...