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Keywords: cryo-electron microscopy
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Biochem J (2021) 478 (24): 4169–4185.
Published: 16 December 2021
... 4.0 (CC BY) . computational biochemistry cryo-electron microscopy imaging techniques structural biology Structure determination of biological macromolecules through single-particle analysis of cryo-EM images has recently reached a milestone by obtaining atomic resolution...
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Biochem J (2021) 478 (17): 3253–3263.
Published: 07 September 2021
...Pu Qian; Tristan I. Croll; David J.K. Swainsbury; Pablo Castro-Hartmann; Nigel W. Moriarty; Kasim Sader; C. Neil Hunter The reaction centre light-harvesting 1 (RC–LH1) complex is the core functional component of bacterial photosynthesis. We determined the cryo-electron microscopy (cryo-EM...
Includes: Supplementary data
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Biochem J (2021) 478 (17): 3185–3204.
Published: 07 September 2021
... by p37 and NU is affected by D1–D2 linker mutations to some extent. anti-caner cryo-electron microscopy enzyme kinetics p97/VCP The N–D1 linker (residues 185–210) and the D1–D2 linker (residues 460–481) connect NTD–D1 and D1–D2 domains, respectively (Figure 1A). This structural...
Includes: Supplementary data
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Biochem J (2021) 478 (16): 3099–3123.
Published: 26 August 2021
... and Huntington-interacting protein 1-related protein (HIP1R), both of which are known to bind to actin, clathrin and PI(4,5)P 2 [ 111–115 ]. Correspondence: Corinne J. Smith ([email protected]) clathrin cryo-electron microscopy endocytosis Figure 3. Stages in clathrin...
Articles
Biochem J (2021) 478 (12): 2371–2384.
Published: 23 June 2021
... 10.1073/pnas.1621051114 17 Zheng , S.Q. , Palovcak , E. , Armache , J.P. , Verba , K.A. , Cheng , Y. and Agard , D.A. (2017) Motioncor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy . Nat. Methods 14 , 331 – 332 10.1038/nmeth...
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Biochem J (2021) 478 (10): 1827–1845.
Published: 18 May 2021
... BY) . Recent developments in cryo-electron microscopy (EM) have enabled the determination of structures of macromolecules at atomic resolution using single-particle analysis (SPA) [ 1 , 2 ]. Obtaining atomic and near-atomic resolution enhances our understanding of the precise mechanisms of protein function...
Articles
Biochem J (2020) 477 (19): 3769–3790.
Published: 12 October 2020
... cations. Correspondence: Rosa Laura López-Marqués ( [email protected] ) 2 7 2020 2 9 2020 16 9 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 autoinhibition CDC50 protein cryo-electron microscopy flippases lipid...
Articles
Biochem J (2020) 477 (14): 2697–2714.
Published: 29 July 2020
... mM MgCl 2 in some cases) and 300 mM NaCl. cryo-electron microscopy membranes nucleotide septin At the plasma membrane, in budding yeast Saccharomyces cerevisiae , septins assemble into a collar-shaped structure at the bud neck during cell division ( Figure 1A ). The collar splits...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2015) 466 (1): 77–84.
Published: 06 February 2015
...) channels, and some but not all of the four subunits of the HCN channel need to be glycosylated for trafficking to cell membrane. cryo-electron microscopy HCN2 fluorescence ion channel membrane reconstitution membrane trafficking N-linked glycosylation The hyperpolarization-activated...
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Biochem J (2012) 445 (2): 157–166.
Published: 27 June 2012
... of seven TM (transmembrane) helices, with a cytoplasmic C-terminus. In detergent, UAC Bc exists largely as a hexamer, as demonstrated by both cross-linking and size-exclusion chromatography. A 9 Å (1 Å=0.1 nm) resolution projection map obtained by cryo-electron microscopy of two-dimensional crystals shows...
Includes: Supplementary data
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Biochem J (2012) 444 (2): 205–209.
Published: 11 May 2012
... © The Authors Journal compilation © 2012 Biochemical Society 2012 cryo-electron microscopy GTPase centre ribosomal P protein ribosomal stalk ribosome small-angle X-ray scattering (SAXS) At all stages of protein synthesis the ribosome requires protein factors, known as tGTPases...
Articles
Biochem J (2008) 410 (2): 261–270.
Published: 12 February 2008
... T2023-GFP and was subsequently used as a basis for three-dimensional reconstruction. Cryo-electron microscopy and single particle image processing of the purified RyR2(A4860G) T2023-GFP protein revealed the location of the inserted GFP, and hence the Ser 2030 PKA site in domain 4, a region that may...