... 4.0 (CC BY) . computational biochemistry cryo-electron microscopy imaging techniques structural biology Structure determination of biological macromolecules through single-particle analysis of cryo-EM images has recently reached a milestone by obtaining atomic resolution...

...Pu Qian; Tristan I. Croll; David J.K. Swainsbury; Pablo Castro-Hartmann; Nigel W. Moriarty; Kasim Sader; C. Neil Hunter The reaction centre light-harvesting 1 (RC–LH1) complex is the core functional component of bacterial photosynthesis. We determined the cryo-electron microscopy (cryo-EM...

... by p37 and NU is affected by D1–D2 linker mutations to some extent. anti-caner cryo-electron microscopy enzyme kinetics p97/VCP The N–D1 linker (residues 185–210) and the D1–D2 linker (residues 460–481) connect NTD–D1 and D1–D2 domains, respectively (Figure 1A). This structural...

... and Huntington-interacting protein 1-related protein (HIP1R), both of which are known to bind to actin, clathrin and PI(4,5)P 2 [ 111–115 ]. Correspondence: Corinne J. Smith ([email protected]) clathrin cryo-electron microscopy endocytosis Figure 3. Stages in clathrin...

... 10.1073/pnas.1621051114 17 Zheng , S.Q. , Palovcak , E. , Armache , J.P. , Verba , K.A. , Cheng , Y. and Agard , D.A. (2017) Motioncor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy . Nat. Methods 14 , 331 – 332 10.1038/nmeth...

... BY) . Recent developments in cryo-electron microscopy (EM) have enabled the determination of structures of macromolecules at atomic resolution using single-particle analysis (SPA) [ 1 , 2 ]. Obtaining atomic and near-atomic resolution enhances our understanding of the precise mechanisms of protein function...

... cations. Correspondence: Rosa Laura López-Marqués ( [email protected] ) 2 7 2020 2 9 2020 16 9 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 autoinhibition CDC50 protein cryo-electron microscopy flippases lipid...

... mM MgCl 2 in some cases) and 300 mM NaCl. cryo-electron microscopy membranes nucleotide septin At the plasma membrane, in budding yeast Saccharomyces cerevisiae , septins assemble into a collar-shaped structure at the bud neck during cell division ( Figure 1A ). The collar splits...

...) channels, and some but not all of the four subunits of the HCN channel need to be glycosylated for trafficking to cell membrane. cryo-electron microscopy HCN2 fluorescence ion channel membrane reconstitution membrane trafficking N-linked glycosylation The hyperpolarization-activated...

... of seven TM (transmembrane) helices, with a cytoplasmic C-terminus. In detergent, UAC Bc exists largely as a hexamer, as demonstrated by both cross-linking and size-exclusion chromatography. A 9 Å (1 Å=0.1 nm) resolution projection map obtained by cryo-electron microscopy of two-dimensional crystals shows...

... © The Authors Journal compilation © 2012 Biochemical Society 2012 cryo-electron microscopy GTPase centre ribosomal P protein ribosomal stalk ribosome small-angle X-ray scattering (SAXS) At all stages of protein synthesis the ribosome requires protein factors, known as tGTPases...

... T2023-GFP and was subsequently used as a basis for three-dimensional reconstruction. Cryo-electron microscopy and single particle image processing of the purified RyR2(A4860G) T2023-GFP protein revealed the location of the inserted GFP, and hence the Ser 2030 PKA site in domain 4, a region that may...