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Keywords: cullin ligase
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Articles
Reconstitution of human CMG helicase ubiquitylation by CUL2 LRR1 and multiple E2 enzymes
Open Access
Journal:
Biochemical Journal
Biochem J (2021) 478 (14): 2825–2842.
Published: 23 July 2021
... that cullin ligases also drive CMG-MCM7 ubiquitylation in human cells, thereby making the helicase into a substrate for the p97 unfoldase. Using purified human proteins, including a panel of E2 ubiquitin-conjugating enzymes, we have reconstituted CMG helicase ubiquitylation, dependent upon neddylated CUL2...
Includes: Supplementary data
