... with CsA (cyclosporin A) before the addition of Ca 2+ . In energized and de-energized mitochondria, Ca 2+ induced MPT regardless of the anion present, although the magnitude differed between P i , As i and V i . However, in all cases, pre-treatment with CsA significantly inhibited MPT. Moreover...

... became pyknotic and caspases were activated. The activities of caspase-3 and caspase-6 (effector caspases) were higher than those of caspase-8 and caspase-9 (initiator caspases). The activation of all four caspases was inhibited by cyclosporin A, with the order of susceptibility caspase-8=caspase-9...

..., and is mediated by FKBP12.6. The Ca 2+ -dependent interaction between FKBP12.6 and the RyR was also found by co-immunoprecipitation. Effects of calcineurin inhibitors were tested on neonatal-rat-heart cardiomyocytes. Treatment of neonatal cardiomyocytes with 20 µ M deltamethrin, 10 µ M cyclosporin A (CsA), or 10...

... of spermine is not affected by cyclosporin A, differently from the effect of permeability transition inducers. In a cardiac cell-free model of apoptosis, the latent caspase activity of cytosolic extracts from cardiomyocytes could be activated by cytochrome c released from spermine-treated heart mitochondria...

...Veronica DOYLE; Sukaina VIRJI; Martin CROMPTON Cyclophilin-A is the cytosolic isoform of a family of peptidylproline cis - trans -isomerases that bind cyclosporin A. This study investigates the role of cyclophilin-A in necrotic cell death, induced by ‘chemical ischaemia’ and by t-butylhydroperoxide...