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Keywords: cytochrome P450
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Articles
Biochem J (2021) 478 (2): 377–388.
Published: 27 January 2021
...J. Patrick Connick; James R. Reed; George F. Cawley; Wayne L. Backes P450 and heme oxygenase-1 (HO-1) receive their necessary electrons by interaction with the NADPH-cytochrome P450 reductase (POR). As the POR concentration is limiting when compared with P450 and HO-1, they must effectively compete...
Includes: Supplementary data
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Biochem J (2020) 477 (2): 541–555.
Published: 31 January 2020
... License 4.0 (CC BY) . Open access for this article was enabled by the participation of University of New South Wales in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with CAUL. cholesterol cytochrome P450 DHCR24...
Includes: Supplementary data
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Biochem J (2019) 476 (23): 3661–3685.
Published: 12 December 2019
...Nadezhda Y. Davydova; Bikash Dangi; Marc A. Maldonado; Nikita E. Vavilov; Victor G. Zgoda; Dmitri R. Davydov In this study, we investigate the ability of ethanol-inducible CYP2E1 to interact with other cytochrome P450 species and affect the metabolism of their substrates. As a model system, we used...
Includes: Supplementary data
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Biochem J (2019) 476 (21): 3313–3331.
Published: 11 November 2019
... cytochrome p450 enzyme activity estrogens mass spectrometry AROM in the temporal cortex and hippocampus is localized in neurons and astrocytes [ 13 – 15 ]. Regulation of AROM activity by phosphorylation (RAAP) in the auditory cortex of songbirds, rapid estrogen synthesis (RES) (the so-called ‘E2...
Includes: Supplementary data
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Biochem J (2018) 475 (23): 3875–3886.
Published: 12 December 2018
... because enzymes need to overcome a high energy barrier. Our structural and computational analysis revealed how a member of the cytochrome P450 family evolved to oxidize a carbohydrate ligand. Using structural biology, we ascertained the molecular determinants of substrate specificity and revealed a highly...
Includes: Supplementary data
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Biochem J (2018) 475 (17): 2801–2817.
Published: 11 September 2018
...Ilona K. Jóźwik; Martin Litzenburger; Yogan Khatri; Alexander Schifrin; Marco Girhard; Vlada Urlacher; Andy-Mark W.H. Thunnissen; Rita Bernhardt Oxidative biocatalytic reactions performed by cytochrome P450 enzymes (P450s) are of high interest for the chemical and pharmaceutical industries...
Includes: Supplementary data
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Biochem J (2018) 475 (13): 2167–2177.
Published: 05 July 2018
... transformation involves combined demethylation and γ-lactone ring formation, catalyzed in bacteria by the cytochrome P450 CYP112 in three individual steps, which involves large structural changes in the transition from substrate to product, with further divergence in the recently demonstrated use of two separate...
Includes: Supplementary data
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Biochem J (2018) 475 (4): 705–722.
Published: 16 February 2018
...Stella A. Child; Elise F. Naumann; John B. Bruning; Stephen G. Bell Members of the cytochrome P450 monooxygenase family CYP268 are found across a broad range of Mycobacterium species including the pathogens Mycobacterium avium , M. colombiense , M. kansasii , and M . marinum . CYP268A2, from M...
Includes: Supplementary data
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Biochem J (2017) 474 (20): 3523–3542.
Published: 10 October 2017
...Dmitri R. Davydov; Nadezhda Y. Davydova; John T. Rodgers; Thomas H. Rushmore; Jeffrey P. Jones Functional cross-talk among human drug-metabolizing cytochrome P450 through their association is a topic of emerging importance. Here, we studied the interactions of human CYP2D6, a major metabolizer of...
Articles
Biochem J (2017) 474 (19): 3241–3252.
Published: 15 September 2017
...Ji Won Park; Aria Byrd; Choon-myung Lee; Edward T. Morgan Nitric oxide (NO) is known to down-regulate drug-metabolizing cytochrome P450 enzymes in an enzyme-selective manner. Ubiquitin–proteasome-dependent and -independent pathways have been reported. Here, we studied the regulation of expression...
Includes: Supplementary data
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Biochem J (2015) 468 (1): 25–31.
Published: 05 May 2015
...Yi Jin; Mo Chen; Trevor M. Penning; Walter L. Miller Cytochrome P450 oxidoreductase (POR) is a 2-flavin protein that transfers electrons from NADPH via its FAD and FMN moieties to all microsomal cytochrome P450 enzymes, including steroidogenic and drug-metabolizing P450s. Defects in the POR gene...
Articles
Biochem J (2015) 467 (1): 1–15.
Published: 20 March 2015
...James B.Y.H. Behrendorff; Weiliang Huang; Elizabeth M.J. Gillam Cytochrome P450 enzymes are renowned for their ability to insert oxygen into an enormous variety of compounds with a high degree of chemo- and regio-selectivity under mild conditions. This property has been exploited in Nature for an...
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Biochem J (2014) 464 (2): 241–249.
Published: 14 November 2014
...Ji Won Park; James R. Reed; Lauren M. Brignac-Huber; Wayne L. Backes Cytochrome P450 (P450) function is dependent on the ability of these enzymes to successfully interact with their redox partners, NADPH-cytochrome P450 reductase (CPR) and cytochrome b 5 , in the endoplasmic reticulum (ER). Because...
Includes: Supplementary data
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Biochem J (2014) 464 (1): 61–71.
Published: 23 October 2014
... used to model key aspects of ischaemia/reperfusion injury. A genetic suppressor screen demonstrated a direct causal role of CYP (cytochrome P450)-13A12 in this response and suggested that CYP-eicosanoids, which in mammals influence the contractility of cardiomyocytes and vascular smooth muscle cells...
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Biochem J (2014) 460 (2): 247–259.
Published: 13 May 2014
.... These high value products are complex to synthesize, but are increasingly important in drug safety testing. The vast majority of drugs are metabolized by cytochromes P450 (P450s), with oxidative transformations usually being highly regio - and stereo -selective. The PPIs (proton pump inhibitors) are...
Includes: Supplementary data
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Biochem J (2013) 454 (2): 209–216.
Published: 09 August 2013
...Yisheng Wu; Qiang Wang; Matthew L. Hillwig; Reuben J. Peters Natural products biosynthesis often requires the action of multiple CYPs (cytochromes P450), whose ability to introduce oxygen, increasing solubility, is critical for imparting biological activity. In previous investigations of rice...
Articles
Biochem J (2012) 446 (3): 489–497.
Published: 28 August 2012
... affect P450 function. To address this problem, the catalytic activities of several P450s were examined in reconstituted systems containing NADPH–POR (cytochrome P450 reductase) and a single P450. CYP2B4 (cytochrome P450 2B4)-, CYP2E1 (cytochrome P450 2E1)- and CYP1A2 (cytochrome P450 1A2)-mediated...
Includes: Supplementary data
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Biochem J (2012) 445 (3): 377–382.
Published: 13 July 2012
...Haiyan Sun; Choon-myung Lee; Shweta Tripathi; Kyung-Bo Kim; Edward T. Morgan CYP2B proteins in rat hepatocytes undergo NO-dependent proteolytic degradation, but the mechanisms and the reasons for the specificity towards only certain P450 (cytochrome P450) enzymes are yet unknown. In the present...
Includes: Supplementary data
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Biochem J (2011) 435 (3): 689–700.
Published: 13 April 2011
...Mandy Kosel; Waltraud Wild; Alexandra Bell; Michael Rothe; Carsten Lindschau; Christian E. W. Steinberg; Wolf-Hagen Schunck; Ralph Menzel Caenorhabditis elegans harbours several CYP (cytochrome P450) genes that are homologous with mammalian CYP isoforms important to the production of...
Includes: Supplementary data
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Biochem J (2011) 433 (1): 85–93.
Published: 15 December 2010
...Wen Yang; Stephen G. Bell; Hui Wang; Weihong Zhou; Mark Bartlam; Luet-Lok Wong; Zihe Rao The cytochrome P450 CYP101D2 from Novosphingobium aromaticivorans DSM12444 is closely related to CYP101D1 from the same bacterium and to P450cam (CYP101A1) from Pseudomonas putida . All three are capable of...
Includes: Supplementary data
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Biochem J (2010) 432 (3): 485–494.
Published: 25 November 2010
...Yassar Farooq; Gordon C. K. Roberts We have incorporated CYP3A4 (cytochrome P450 3A4) and CPR (NADPH-cytochrome P450 reductase) into liposomes with a high lipid/protein ratio by an improved method. In the purified proteoliposomes, CYP3A4 binds testosterone with K d (app)=36±6 μM and Hill...
Includes: Supplementary data
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Biochem J (2010) 432 (3): 505–516.
Published: 25 November 2010
... cytochrome P450s and UGTs (UDP-glucuronosyl transferases). The most highly induced genes are predominantly expressed in the worm intestine, supporting their role in drug metabolism. HPLC-MS/MS revealed the production of two novel glucoside metabolites in C. elegans identifying a major difference in the...
Includes: Supplementary data
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Biochem J (2010) 431 (3): 337–347.
Published: 11 October 2010
...Dana Morrone; Xiaoming Chen; Robert M. Coates; Reuben J. Peters KO (kaurene oxidase) is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4α methyl of the ent -kaurene olefin intermediate into the...
Includes: Supplementary data
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Biochem J (2010) 429 (3): 435–449.
Published: 14 July 2010
... and succinylcholine. The subject area developed rapidly, particularly with regard to genetic factors affecting drug disposition. There is now comprehensive understanding of the molecular basis for variable drug metabolism by the cytochromes P450 and also for variable glucuronidation, acetylation and...
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Biochem J (2010) 427 (3): 455–466.
Published: 14 April 2010
...Hazel M. Girvan; Colin W. Levy; Paul Williams; Karl Fisher; Myles R. Cheesman; Stephen E. J. Rigby; David Leys; Andrew W. Munro Bacillus megaterium flavocytochrome P450 BM3 (CYP102A1) is a biotechnologically important cytochrome P450/P450 reductase fusion enzyme. Mutants I401E, F261E and L86E were...
Includes: Supplementary data
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Biochem J (2009) 417 (1): 43–58.
Published: 12 December 2008
...Robert D. Finn; Colin J. Henderson; Claire L. Scott; C. Roland Wolf The liver is responsible for key metabolic functions, including control of normal homoeostasis in response to diet and xenobiotic metabolism/detoxification. We have shown previously that inactivation of the hepatic cytochrome P450...
Includes: Supplementary data
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Biochem J (2009) 417 (1): 65–80.
Published: 12 December 2008
...–cytochrome P450 reductase and P450 domains. Selected mutations at residue 264 in the haem (P450) domain of the enzyme lead to novel amino acid sixth (distal) co-ordination ligands to the haem iron. The catalytic, spectroscopic and thermodynamic properties of the A264M, A264Q and A264C variants were...
Includes: Supplementary data
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Biochem J (2007) 403 (1): 109–118.
Published: 13 March 2007
... function. To identify the CYP (cytochrome P450) isoforms catalysing this reaction in the mouse kidney, we analysed the substrate specificity of Cyp4a10, 4a12a, 4a12b and 4a14 and determined sex- and strain-specific expressions. All recombinant enzymes showed high lauric acid hydroxylase activities...
Articles
Biochem J (2006) 396 (2): 235–242.
Published: 15 May 2006
... 2005 23 1 2006 20 2 2006 20 2 2006 The Biochemical Society, London 2006 antiangiogenesis anticancer agent cytochrome P450 microtubule tubulin tubulysin A Most drugs currently available for the treatment of cancer are mechanistically based on the inhibition of...
Includes: Supplementary data
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Biochem J (2006) 395 (3): 641–652.
Published: 11 April 2006
...Richard K. Hughes; Eric J. Belfield; Mylrajan Muthusamay; Anuja Khan; Arthur Rowe; Stephen E. Harding; Shirley A. Fairhurst; Stephen Bornemann; Ruth Ashton; Roger N. F. Thorneley; Rod Casey We describe the detailed biochemical characterization of CYP74C3 (cytochrome P450 subfamily 74C3), a...
Includes: Supplementary data
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Biochem J (2006) 393 (3): 635–643.
Published: 13 January 2006
...Aike Stortelder; Peter H. J. Keizers; Chris Oostenbrink; Chris De Graaf; Petra De Kruijf; Nico P. E. Vermeulen; Cees Gooijer; Jan N. M. Commandeur; Gert Van Der Zwan Enzyme structure and dynamics may play a main role in substrate binding and the subsequent steps in the CYP (cytochrome P450...
Includes: Supplementary data
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Biochem J (2005) 391 (3): 631–640.
Published: 25 October 2005
...Nicole Y. Marden; Michael Murray The human cytochrome P450 2J2 (CYP2J2) generates cytoprotective epoxyeicosatrienoic acids from arachidonic acid. Expression of CYP2J2 is decreased in hypoxia, and the resultant decrease in CYP2J2-derived epoxyeicosanoids may contribute to the pathogenesis of cardiac...
Articles
Biochem J (2005) 390 (3): 719–727.
Published: 05 September 2005
... the three cis bonds for oxidative metabolism mediated by CYP (cytochrome P450) epoxygenase and hydroxylase. This was evidenced by the detection of 5,6- trans -EET (where EET stands for epoxyeicosatrienoic acid), 5,6- erythro -dihydroxyeicosatrienoic acid and an isomer of 5-HETE. A standard of 5,6...
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Biochem J (2005) 388 (3): 857–867.
Published: 07 June 2005
...Xiu Jun WANG; Mark CHAMBERLAIN; Olga VASSIEVA; Colin J. HENDERSON; C. Roland WOLF Cytochrome P450 reductase is the unique electron donor for microsomal cytochrome P450s; these enzymes play a major role in the metabolism of endogenous and xenobiotic compounds. In mice with a liver-specific deletion...
Includes: Supplementary data
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Biochem J (2004) 381 (3): 887–894.
Published: 27 July 2004
... DDBJ, EMBL, GenBank® and GSDB Nucleotide Sequence Databases under the accession number AY321510. 10 3 2004 28 4 2004 29 4 2004 29 4 2004 The Biochemical Society, London 2004 cytochrome P450 hepatocyte nuclear factor 4 (HNF-4) liver nuclear factor I (NF-I...
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Biochem J (2004) 380 (2): 353–360.
Published: 01 June 2004
...Jack U. FLANAGAN; Jean-Didier MARÉCHAL; Richard WARD; Carol A. KEMP; Lesley A. McLAUGHLIN; Michael J. SUTCLIFFE; Gordon C. K. ROBERTS; Mark J. I. PAINE; C. Roland WOLF Although the residues that determine the preference of CYP2D6 (cytochrome P450 2D6) for compounds containing a basic nitrogen are...
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Biochem J (2003) 373 (3): 669–680.
Published: 01 August 2003
...Nicole Y. MARDEN; Eva FIALA-BEER; Shi-Hua XIANG; Michael MURRAY The cytochrome P450 (CYP) 2J2 arachidonic acid epoxygenase gene was down-regulated at a pre-translational level in human hepatoma-derived HepG2 cells incubated in a hypoxic environment; under these conditions, the expression of c-Jun...
Articles
Biochem J (2003) 370 (3): 763–769.
Published: 15 March 2003
... also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible...
Articles
Biochem J (2002) 368 (3): 721–728.
Published: 15 December 2002
...Ke-He RUAN; Shui-Ping SO; Weida ZHENG; Jiaxin WU; Dawei LI; Jennifer KUNG The three-dimensional structure of a synthetic peptide corresponding to the N-terminal membrane anchor domain (residues 1—25) of prostaglandin I 2 synthase (also known as cytochrome P450 8A1), an eicosanoid-synthesizing...
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Biochem J (2002) 364 (2): 555–562.
Published: 01 June 2002
...David C. LAMB; Kay FOWLER; Tobias KIESER; Nigel MANNING; Larissa M. PODUST; Michael R. WATERMAN; Diane E. KELLY; Steven L. KELLY The annotation of the genome sequence of Streptomyces coelicolor A3(2) revealed a cytochrome P450 (CYP) resembling various sterol 14α-demethylases (CYP51). The putative...
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Biochem J (2002) 362 (3): 545–551.
Published: 08 March 2002
..., London ©2002 2002 cytochrome P450 membrane topology peptide antibody prostaglandin prostaglandin I 2 synthase Abbreviations used: ER, endoplasmic reticulum; PGIS, prostaglandin I 2 synthase; TXAS, thromboxane A 2 synthase; PGHS, prostaglandin H 2 (PGH 2 ) synthase; SLO...
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Biochem J (2001) 355 (2): 509–515.
Published: 06 April 2001
.... We recently described a novel cytochrome P450 enzyme, cyp7b1, strongly expressed in the hippocampus of rodent brain, which catalyses the metabolism of DHEA, pregnenolone and 25-hydroxycholesterol to 7α-hydroxy products. In the light of this new enzyme, we have examined its possible role in 7α...