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Articles

Tweaking the redox properties of PpcA from Geobacter metallireducens with protein engineering

Pilar C. Portela, Marta A. Silva, Alexandre Almeida, Gonçalo F. Damas, Carlos A. Salgueiro

Biochem J (2024) 481 (24): 2017–2036.

https://doi.org/10.1042/BCJ20240423

Published: 23 December 2024

... in this bacteria was named PpcF [ 26 ]. cytochrome C electron transfer Geobacter NMR spectroscopy protein engineering As previously mentioned, the mutants were selected based on their E app values. However, to determine the complete set of thermodynamic parameters, namely the individual haem...

View articletitled, Tweaking the redox properties of PpcA from Geobacter metallireducens with protein engineering

Includes: Supplementary data

Articles

Altered structure and dynamics of pathogenic cytochrome c variants correlate with increased apoptotic activity

Matthias Fellner, Rinky Parakra, Kirstin O. McDonald, Itamar Kass, Guy N.L. Jameson, Sigurd M. Wilbanks, Elizabeth C. Ledgerwood

Biochem J (2021) 478 (3): 669–684.

https://doi.org/10.1042/BCJ20200793

Published: 12 February 2021

...Matthias Fellner; Rinky Parakra; Kirstin O. McDonald; Itamar Kass; Guy N.L. Jameson; Sigurd M. Wilbanks; Elizabeth C. Ledgerwood Mutation of cytochrome c in humans causes mild autosomal dominant thrombocytopenia. The role of cytochrome c in platelet formation, and the molecular mechanism underlying...

View articletitled, Altered structure and dynamics of pathogenic cytochrome c variants correlate with increased apoptotic activity

Includes: Supplementary data

Articles

Osmotic pressure effects identify dehydration upon cytochrome c –cytochrome c oxidase complex formation contributing to a specific electron pathway formation

Wataru Sato, Seiji Hitaoka, Takeshi Uchida, Kyoko Shinzawa-Itoh, Kazunari Yoshizawa, Shinya Yoshikawa, Koichiro Ishimori

Biochem J (2020) 477 (8): 1565–1578.

https://doi.org/10.1042/BCJ20200023

Published: 29 April 2020

...Wataru Sato; Seiji Hitaoka; Takeshi Uchida; Kyoko Shinzawa-Itoh; Kazunari Yoshizawa; Shinya Yoshikawa; Koichiro Ishimori In the electron transfer (ET) reaction from cytochrome c (Cyt c ) to cytochrome c oxidase (C c O), we determined the number and sites of the hydration water released from...

View articletitled, Osmotic pressure effects identify dehydration upon cytochrome c –cytochrome c oxidase complex formation contributing to a specific electron pathway formation

Includes: Supplementary data

Articles

The chromogranin A-derived antifungal peptide CGA-N9 induces apoptosis in Candida tropicalis

Ruifang Li, Chen Chen, Beibei Zhang, Hongjuan Jing, Zichao Wang, Chunling Wu, Pu Hao, Yong Kuang, Minghang Yang

Biochem J (2019) 476 (20): 3069–3080.

https://doi.org/10.1042/BCJ20190483

Published: 30 October 2019

...) increased. Accordingly, we assessed apoptotic processes in Candida tropicalis cells post-treatment with CGA-N9 and found cytochrome c leakage, chromatin condensation and DNA degradation. The interaction of CGA-N9 with DNA in vitro showed that CGA-N9 did not degrade DNA but bound to DNA via an electrostatic...

View articletitled, The chromogranin A-derived antifungal peptide CGA-N9 induces apoptosis in Candida tropicalis

Articles

CGA-N12, a peptide derived from chromogranin A, promotes apoptosis of Candida tropicalis by attenuating mitochondrial functions

Ruifang Li, Ruiling Zhang, Yanhui Yang, Xueqin Wang, Yanjie Yi, Pei Fan, Zhengwei Liu, Chen Chen, Junpeng Chang

Biochem J (2018) 475 (7): 1385–1396.

https://doi.org/10.1042/BCJ20170894

Published: 16 April 2018

... membrane potential, in which the former plays key roles in the initiation of apoptosis and the latter is a sign of the cell apoptosis. Accordingly, we assessed the apoptosis features of Candida tropicalis cells after treatment with CGA-N12 and found the following: leakage of cytochrome c and uptake...

View articletitled, CGA-N12, a peptide derived from chromogranin A, promotes apoptosis of Candida tropicalis by attenuating mitochondrial functions

Articles

Biochemical and functional characterization of a periplasmic disulfide oxidoreductase from Neisseria meningitidis essential for meningococcal viability

Adeline Gand, Laure Selme-Roussel, Sabrina Collin, Guy Branlant, Christophe Jacob, Sandrine Boschi-Muller

Biochem J (2015) 468 (2): 271–282.

https://doi.org/10.1042/BJ20140868

Published: 22 May 2015

...Adeline Gand; Laure Selme-Roussel; Sabrina Collin; Guy Branlant; Christophe Jacob; Sandrine Boschi-Muller TlpAs (thioredoxin-like proteins) are bacterial thioredoxin-like periplasmic disulfide oxidoreductases generally involved in cytochrome c maturation (Ccm) process. They contain a characteristic...

View articletitled, Biochemical and functional characterization of a periplasmic disulfide oxidoreductase from Neisseria meningitidis essential for meningococcal viability

Includes: Supplementary data

Articles

Enhancing the peroxidase activity of cytochrome c by mutation of residue 41: implications for the peroxidase mechanism and cytochrome c release

Tracy M. Josephs, Ian M. Morison, Catherine L. Day, Sigurd M. Wilbanks, Elizabeth C. Ledgerwood

Biochem J (2014) 458 (2): 259–265.

https://doi.org/10.1042/BJ20131386

Published: 14 February 2014

...Tracy M. Josephs; Ian M. Morison; Catherine L. Day; Sigurd M. Wilbanks; Elizabeth C. Ledgerwood The peroxidase activity of cytochrome c may play a key role in the release of cytochrome c from the mitochondrial intermembrane space in the intrinsic apoptosis pathway. Induction of the peroxidase...

View articletitled, Enhancing the peroxidase activity of cytochrome c by mutation of residue 41: implications for the peroxidase mechanism and cytochrome c release

Includes: Supplementary data

Articles

The hydrogen-peroxide-induced radical behaviour in human cytochrome c –phospholipid complexes: implications for the enhanced pro-apoptotic activity of the G41S mutant

Badri S. Rajagopal, Ann N. Edzuma, Michael A. Hough, Katie L. I. M. Blundell, Valerian E. Kagan, Alexandr A. Kapralov, Lewis A. Fraser, Julea N. Butt, Gary G. Silkstone, Michael T. Wilson, Dimitri A. Svistunenko, Jonathan A. R. Worrall

Biochem J (2013) 456 (3): 441–452.

https://doi.org/10.1042/BJ20130758

Published: 22 November 2013

... of the G41S mutant of human cytochrome c can be explained by a higher than wild-type peroxidase activity triggered by phospholipid binding. A key complex in mitochondrial apoptosis involves cytochrome c and the phospholipid cardiolipin. In this complex cytochrome c has its native axial Met 80 ligand...

View articletitled, The hydrogen-peroxide-induced radical behaviour in human cytochrome c –phospholipid complexes: implications for the enhanced pro-apoptotic activity of the G41S mutant

Includes: Supplementary data

Articles

Cytochrome c -mediated formation of S -nitrosothiol in cells

Katarzyna A. Broniowska, Agnes Keszler, Swati Basu, Daniel B. Kim-Shapiro, Neil Hogg

Biochem J (2012) 442 (1): 191–197.

https://doi.org/10.1042/BJ20111294

Published: 27 January 2012

..., and no efficient protein-mediated process has been identified. Recently, we observed that ferric cytochrome c can promote S -nitrosoglutathione formation from NO and glutathione by acting as an electron acceptor under anaerobic conditions. In the present study, we show that this mechanism is also robust under...

View articletitled, Cytochrome c -mediated formation of S -nitrosothiol in cells

Articles

The role of oxidized cytochrome c in regulating mitochondrial reactive oxygen species production and its perturbation in ischaemia

Philippe Pasdois, Joanne E. Parker, Elinor J. Griffiths, Andrew P. Halestrap

Biochem J (2011) 436 (2): 493–505.

https://doi.org/10.1042/BJ20101957

Published: 13 May 2011

...Philippe Pasdois; Joanne E. Parker; Elinor J. Griffiths; Andrew P. Halestrap Oxidized cytochrome c is a powerful superoxide scavenger within the mitochondrial IMS (intermembrane space), but the importance of this role in situ has not been well explored. In the present study, we investigated...

View articletitled, The role of oxidized cytochrome c in regulating mitochondrial reactive oxygen species production and its perturbation in ischaemia

Includes: Supplementary data

Articles

Decreased cytochrome c mediates an age-related decline of oxidative phosphorylation in rat kidney mitochondria

John F. O'Toole, Hiral V. Patel, Colin J. Naples, Hisashi Fujioka, Charles L. Hoppel

Biochem J (2010) 427 (1): 105–112.

https://doi.org/10.1042/BJ20091373

Published: 15 March 2010

... 344 rats. OXPHOS (oxidative phosphorylation) of intact mitochondria and cytochrome c oxidase activity in permeabilized mitochondria were determined with polarographic assays. The activities of the ETC (electron transport chain) complexes and the cytochrome content in solubilized mitochondria were...

View articletitled, Decreased cytochrome c mediates an age-related decline of oxidative phosphorylation in rat kidney mitochondria

Includes: Supplementary data

Articles

Thermodynamic characterization of the redox centres in a representative domain of a novel c -type multihaem cytochrome

Leonor Morgado, Ana P. Fernandes, Yuri Y. Londer, P. Raj Pokkuluri, Marianne Schiffer, Carlos A. Salgueiro

Biochem J (2009) 420 (3): 485–492.

https://doi.org/10.1042/BJ20082428

Published: 27 May 2009

... of these proteins, two with 12 haems (GSU1996, GSU0592) and one with 27 haems (GSU2210), suggest that they are formed with domains homologous to the trihaem cytochrome c 7 . Although all three haems have bis-His co-ordination in cytochromes c 7 , in each domain of the above polymers, the haem equivalent to haem IV...

View articletitled, Thermodynamic characterization of the redox centres in a representative domain of a novel c -type multihaem cytochrome

Includes: Supplementary data

Articles

Cytochrome c impaled: investigation of the extended lipid anchorage of a soluble protein to mitochondrial membrane models

Erta Kalanxhi, Carmichael J. A. Wallace

Biochem J (2007) 407 (2): 179–187.

https://doi.org/10.1042/BJ20070459

Published: 25 September 2007

...Erta Kalanxhi; Carmichael J. A. Wallace Cyt c (cytochrome c ) has been traditionally envisioned as rapidly diffusing in two dimensions at the surface of the mitochondrial inner membrane when not engaged in redox reactions with physiological partners. However, the discovery of the extended lipid...

View articletitled, Cytochrome c impaled: investigation of the extended lipid anchorage of a soluble protein to mitochondrial membrane models

Articles

Requirement of Apaf-1 for mitochondrial events and the cleavage or activation of all procaspases during genotoxic stress-induced apoptosis

Emily E. Franklin, John D. Robertson

Biochem J (2007) 405 (1): 115–122.

https://doi.org/10.1042/BJ20061576

Published: 13 June 2007

...Emily E. Franklin; John D. Robertson Sequential activation of caspases is critical for the execution of apoptosis. Recent evidence suggests caspase 2 is a significant upstream caspase capable of initiating mitochondrial events, such as the release of cytochrome c . In particular, in vitro studies...

View articletitled, Requirement of Apaf-1 for mitochondrial events and the cleavage or activation of all procaspases during genotoxic stress-induced apoptosis

Articles

Reduction of ferric haemoproteins by tetrahydropterins: a kinetic study

Chantal Capeillere-Blandin, Delphine Mathieu, Daniel Mansuy

Biochem J (2005) 392 (3): 583–587.

https://doi.org/10.1042/BJ20050437

Published: 06 December 2005

... and cytochrome c , there was a good correlation between the second-order reduction rate constant and the redox potential, E m (Fe III /Fe II ): The rate of reduction of cytochrome c by BH 4 [(6 R )-5,6,7,8-tetrahydrobiopterin] was determined to be similar to that of the reduction of cytochrome c by diMePH 4...

View articletitled, Reduction of ferric haemoproteins by tetrahydropterins: a kinetic study

Articles

Suppression of the pro-apoptotic function of cytochrome c by singlet oxygen via a haem redox state-independent mechanism

Daisuke Suto, Kazuaki Sato, Yoshihiro Ohba, Tetsuhiko Yoshimura, Junichi Fujii

Biochem J (2005) 392 (2): 399–406.

https://doi.org/10.1042/BJ20050580

Published: 22 November 2005

...Daisuke Suto; Kazuaki Sato; Yoshihiro Ohba; Tetsuhiko Yoshimura; Junichi Fujii Stimuli for apoptotic signalling typically induce release of cyt c (cytochrome c ) from mitochondria. Cyt c then initiates the formation of the apoptosome, comprising Apaf-1 (apoptotic protease-activating factor 1...

View articletitled, Suppression of the pro-apoptotic function of cytochrome c by singlet oxygen via a haem redox state-independent mechanism

Includes: Supplementary data

Articles

An abortive apoptotic pathway induced by singlet oxygen is due to the suppression of caspase activation

Kaoru OTSU, Kazuaki SATO, Yoshitaka IKEDA, Hirotaka IMAI, Yasuhito NAKAGAWA, Yoshihiro OHBA, Junichi FUJII

Biochem J (2005) 389 (1): 197–206.

https://doi.org/10.1042/BJ20042067

Published: 21 June 2005

... the release of cytochrome c from mitochondria into the cytosol and exhibited cytotoxicity, but NDPE did not. RBL cells, a rat basophil leukaemia-derived line, that overexpress phospholipid hydroperoxide glutathione peroxidase in mitochondria were found to be highly resistant to the cytotoxic effect of MNPE...

View articletitled, An abortive apoptotic pathway induced by singlet oxygen is due to the suppression of caspase activation

Articles

Oligomeric states of the voltage-dependent anion channel and cytochrome c release from mitochondria

Ran ZALK, Adrian ISRAELSON, Erez S. GARTY, Heftsi AZOULAY-ZOHAR, Varda SHOSHAN-BARMATZ

Biochem J (2005) 386 (1): 73–83.

https://doi.org/10.1042/BJ20041356

Published: 08 February 2005

...Ran ZALK; Adrian ISRAELSON; Erez S. GARTY; Heftsi AZOULAY-ZOHAR; Varda SHOSHAN-BARMATZ The VDAC (voltage-dependent anion channel) plays a central role in apoptosis, participating in the release of apoptogenic factors including cytochrome c . The mechanisms by which VDAC forms a protein-conducting...

View articletitled, Oligomeric states of the voltage-dependent anion channel and cytochrome c release from mitochondria

Articles

A novel role of Mgm1p, a dynamin-related GTPase, in ATP synthase assembly and cristae formation/maintenance

Boominathan AMUTHA, Donna M. GORDON, Yajuan GU, Debkumar PAIN

Biochem J (2004) 381 (1): 19–23.

https://doi.org/10.1042/BJ20040566

Published: 22 June 2004

... protein stability, ATP synthase assembly, cristae morphology and cytochrome c storage within cristae. 1 To whom correspondence should be addressed (e-mail [email protected] ). 5 5 2004 6 4 2004 30 4 2004 5 5 2004 The Biochemical Society, London 2004 ATP synthase...

View articletitled, A novel role of Mgm1p, a dynamin-related GTPase, in ATP synthase assembly and cristae formation/maintenance

Articles

Calcineurin-mediated Bad translocation regulates cyanide-induced neuronal apoptosis

Yan SHOU, Li LI, Krishnan PRABHAKARAN, Joseph L. BOROWITZ, Gary E. ISOM

Biochem J (2004) 379 (3): 805–813.

https://doi.org/10.1042/bj20031107

Published: 01 May 2004

... of Bax (Bcl-associated X protein) to mitochondria is linked with release of cytochrome c and subsequent activation of a caspase cascade [Shou, Li, Prabhakaran, Borowitz and Isom (2003) Toxicol. Sci. 75 , 99–107]. In the present study, the relationship of the cyanide-induced increase in cytosolic free Ca...

View articletitled, Calcineurin-mediated Bad translocation regulates cyanide-induced neuronal apoptosis

Articles

Bax-induced cytochrome c release from mitochondria depends on alpha-helices-5 and -6

Gerd HEIMLICH, Alastair D. McKINNON, Katussevani BERNARDO, Dieter BRDICZKA, John C. REED, Renate KAIN, Martin KRÖNKE, Juliane M. JÜRGENSMEIER

Biochem J (2004) 378 (1): 247–255.

https://doi.org/10.1042/bj20031152

Published: 15 February 2004

... and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol. However, the domains of Bax that are essential for the induction of cytochrome c release are still elusive. Therefore various Bax deletion mutants were generated...

View articletitled, Bax-induced cytochrome c release from mitochondria depends on alpha-helices-5 and -6

Articles

Mitochondrial cytochrome c release may occur by volume-dependent mechanisms not involving permeability transition

Vladimir GOGVADZE, John D. ROBERTSON, Mari ENOKSSON, Boris ZHIVOTOVSKY, Sten ORRENIUS

Biochem J (2004) 378 (1): 213–217.

https://doi.org/10.1042/bj20031193

Published: 15 February 2004

...Vladimir GOGVADZE; John D. ROBERTSON; Mari ENOKSSON; Boris ZHIVOTOVSKY; Sten ORRENIUS The mechanisms regulating mitochondrial outer-membrane permeabilization and the release of cytochrome c during apoptosis remain controversial. In the present study, we show in an in vitro model system...

View articletitled, Mitochondrial cytochrome c release may occur by volume-dependent mechanisms not involving permeability transition

Articles

Cytochrome c is reduced mainly by plastoquinol and not by superoxide in thylakoid membranes at low and medium light intensities: its specific interaction with thylakoid membrane lipids

Jerzy KRUK, Małgorzata JEMIOŁA-RZEMIŃSKA, Kazimierz STRZAŁKA

Biochem J (2003) 375 (1): 215–220.

https://doi.org/10.1042/bj20021820

Published: 01 October 2003

...Jerzy KRUK; Małgorzata JEMIOŁA-RZEMIŃSKA; Kazimierz STRZAŁKA We have found that, at low light intensity (5–10 μmol photons·m −2 ·s −1 ), photoreduction of cyt (cytochrome) c by isolated thylakoids was not inhibited by dinitrophenylether of iodonitrothymol, an inhibitor of the cyt b 6 – f complex...

View articletitled, Cytochrome c is reduced mainly by plastoquinol and not by superoxide in thylakoid membranes at low and medium light intensities: its specific interaction with thylakoid membrane lipids

Articles

c-Jun N-terminal kinase (JNK)-mediated modulation of brain mitochondria function: new target proteins for JNK signalling in mitochondrion-dependent apoptosis

Hagen SCHROETER, Clinton S. BOYD, Ruhi AHMED, Jeremy P. E. SPENCER, Roger F. DUNCAN, Catherine RICE-EVANS, Enrique CADENAS

Biochem J (2003) 372 (2): 359–369.

https://doi.org/10.1042/bj20030201

Published: 01 June 2003

...Hagen SCHROETER; Clinton S. BOYD; Ruhi AHMED; Jeremy P. E. SPENCER; Roger F. DUNCAN; Catherine RICE-EVANS; Enrique CADENAS The molecular mechanisms underlying the initiation and control of the release of cytochrome c during mitochondrion-dependent apoptosis are thought to involve...

View articletitled, c-Jun N-terminal kinase (JNK)-mediated modulation of brain mitochondria function: new target proteins for JNK signalling in mitochondrion-dependent apoptosis

Articles

c-Myc potentiates the mitochondrial pathway of apoptosis by acting upstream of apoptosis signal-regulating kinase 1 (Ask1) in the p38 signalling cascade

Katia M. DESBIENS, Réna G. DESCHESNES, Mireille M. LABRIE, Yan DESFOSSÉS, Herman LAMBERT, Jacques LANDRY, Kerstin BELLMANN

Biochem J (2003) 372 (2): 631–641.

https://doi.org/10.1042/bj20021565

Published: 01 June 2003

... with deregulated expression of c-Myc resulted in nuclear fragmentation that was accompanied in all cells by the activation of Bax and the translocation of cytochrome c from the mitochondria to the cytoplasm. None of these features of apoptosis was induced in control Rat-1 cells. p38 was also activated by cisplatin...

View articletitled, c-Myc potentiates the mitochondrial pathway of apoptosis by acting upstream of apoptosis signal-regulating kinase 1 (Ask1) in the p38 signalling cascade

Articles

Nitric oxide donors inhibit formation of the Apaf-1/caspase-9 apoptosome and activation of caspases

Birgit ZECH, Roman KÖHL, Andreas von KNETHEN, Bernhard BRÜNE

Biochem J (2003) 371 (3): 1055–1064.

https://doi.org/10.1042/bj20021720

Published: 01 May 2003

... caspase activation and apoptosis (downstream of mitochondrial membrane depolarization) and cytochrome c release. However, caspase activity was not restored by the strong reducing agent dithiothreitol, as predicted for S-nitrosation reactions, thereby excluding active-site-thiol modification of caspases...

View articletitled, Nitric oxide donors inhibit formation of the Apaf-1/caspase-9 apoptosome and activation of caspases

Articles

The anti-apoptotic effect of leukotriene D4 involves the prevention of caspase 8 activation and Bid cleavage

Katarina WIKSTRÖM, Maria JUHAS, Anita SJÖLANDER

Biochem J (2003) 371 (1): 115–124.

https://doi.org/10.1042/bj20021669

Published: 01 April 2003

...-nitrophenyl)methane sulphonamide has been shown previously to cause apoptosis in intestinal epithelial cells. In the present study, we attempted to elucidate the underlying mechanisms and we can now show that a mitochondrial pathway is employed. Inhibition of COX-2 causes release of cytochrome c , as shown...

View articletitled, The anti-apoptotic effect of leukotriene D4 involves the prevention of caspase 8 activation and Bid cleavage

Articles

Selenite sensitizes mitochondrial permeability transition pore opening in vitro and in vivo: a possible mechanism for chemo-protection

Shani SHILO, Anna ARONIS, Rita KOMARNITSKY, Oren TIROSH

Biochem J (2003) 370 (1): 283–290.

https://doi.org/10.1042/bj20021022

Published: 15 February 2003

...—10 μ M) strongly induced cyclosporin A-sensitive mitochondrial swelling. Selenium also promoted both calcium release from the matrix of isolated mitochondria and uncoupled respiration. The MPT-inducing effect of selenium provoked the release of cytochrome c , a pro-apoptotic factor...

View articletitled, Selenite sensitizes mitochondrial permeability transition pore opening in vitro and in vivo: a possible mechanism for chemo-protection

Articles

Bax oligomerization in mitochondrial membranes requires tBid (caspase-8-cleaved Bid) and a mitochondrial protein

Xavier ROUCOU, Sylvie MONTESSUIT, Bruno ANTONSSON, Jean-Claude MARTINOU

Biochem J (2002) 368 (3): 915–921.

https://doi.org/10.1042/bj20020972

Published: 15 December 2002

...Xavier ROUCOU; Sylvie MONTESSUIT; Bruno ANTONSSON; Jean-Claude MARTINOU In response to various apoptotic stimuli, Bax, a pro-apoptotic member of the Bcl-2 family, is oligomerized and permeabilizes the mitochondrial outer membrane to apoptogenic factors, including cytochrome c . Bax oligomerization...

View articletitled, Bax oligomerization in mitochondrial membranes requires tBid (caspase-8-cleaved Bid) and a mitochondrial protein

Articles

Cloning, sequencing and heterologous expression of the gene for lupanine hydroxylase, a quinocytochrome c from a Pseudomonas sp.

David J. HOPPER, Mustak A. KADERBHAI, Shirley A. MARRIOTT, Michael YOUNG, Jerzy ROGOZINSKI

Biochem J (2002) 367 (2): 483–489.

https://doi.org/10.1042/bj20020729

Published: 15 October 2002

... the transcriptional control of a phoA promotor and with the Escherichia coli alkaline phosphatase signal sequence, was expressed in E. coli containing a plasmid expressing the genes for cytochrome c maturation proteins constitutively. Haem-containing inactive protein in inclusion bodies was renatured and reactivated...

View articletitled, Cloning, sequencing and heterologous expression of the gene for lupanine hydroxylase, a quinocytochrome c from a Pseudomonas sp.

Articles

Biphasic translocation of Bax to mitochondria

Michela CAPANO, Martin CROMPTON

Biochem J (2002) 367 (1): 169–178.

https://doi.org/10.1042/bj20020805

Published: 01 October 2002

... the cytosol was relatively small. In the second phase, Bax was very largely removed from the cytosol and sequestered into large aggregates associated with the mitochondria. To test which of the phases involved cytochrome c release, cells were transfected with a red fluorescent protein—cytochrome c fusion...

View articletitled, Biphasic translocation of Bax to mitochondria

Articles

Mitochondrial respiratory chain-dependent generation of superoxide anion and its release into the intermembrane space

Derick HAN, Everett WILLIAMS, Enrique CADENAS

Biochem J (2001) 353 (2): 411–416.

https://doi.org/10.1042/bj3530411

Published: 08 January 2001

...-superoxide dismutase. Release of superoxide anion into the intermembrane space is a controversial topic, partly unresolved by the reaction of superoxide anion with cytochrome c , which faces the intermembrane space and is present in this compartment at a high concentration. This study was aimed at assessing...

View articletitled, Mitochondrial respiratory chain-dependent generation of superoxide anion and its release into the intermembrane space

Articles

A possible origin of differences between calorimetric and equilibrium estimates of stability parameters of proteins

Amita SINHA, Sushma YADAV, Rizwan AHMAD, Faizan AHMAD

Biochem J (2000) 345 (3): 711–717.

https://doi.org/10.1042/bj3450711

Published: 25 January 2000

...Amita SINHA; Sushma YADAV; Rizwan AHMAD; Faizan AHMAD To test the validity of thermodynamic parameters from the equilibrium method, we have studied the reversible heat-induced denaturations of lysozyme, ribonuclease A, cytochrome c and myoglobin at various pH values, using absorption spectral...

View articletitled, A possible origin of differences between calorimetric and equilibrium estimates of stability parameters of proteins

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