... of CMS-G, the mitochondrial sterilizing factor causing CMS-G is still unknown. By characterizing biochemically CMS-G, we found that the expression of several mitochondrial proteins is altered in CMS-G plants. In particular, Cox1, a core subunit of the cytochrome c oxidase (complex IV), is larger but can...

...Kateřina Hejzlarová; Vilma Kaplanová; Hana Nůsková; Nikola Kovářová; Pavel Ješina; Zdeněk Drahota; Tomáš Mráček; Sara Seneca; Josef Houštěk MtDNA 9205delTA microdeletion in the MT-ATP6 / MT-CO3 gene alters the structure of ATP synthase, down-regulates the content of cytochrome c oxidase and thus...

...Raksha Dodia; Brigitte Meunier; Christopher W. M. Kay; Peter R. Rich Subunit 5 of Saccharomyces cerevisiae cytochrome c oxidase (C c O) is essential for assembly and has two isoforms, 5A and 5B. 5A is expressed under normoxic conditions, whereas 5B is expressed at very low oxygen tensions...

... A domain of CcO (cytochrome c oxidase). How Sco obtains copper once secreted to the extracytoplasmic environment is unknown. In the present paper we report on a protein possessing an HX 6 MX 21 HXM motif that binds a single cuprous ion with subfemtomolar affinity. High-resolution X-ray structures...

...Brigitte Meunier; Amandine Maréchal; Peter R. Rich Yeast C c O (cytochrome c oxidase) has been developed as a facile system for the production and analysis of mutants of a mitochondrial form of C c O for mechanistic studies. First, a 6H tag (His 6 tag) was fused to the C-terminus of a nuclear...

...Daniela Fornuskova; Lukas Stiburek; Laszlo Wenchich; Kamila Vinsova; Hana Hansikova; Jiri Zeman Mammalian CcO (cytochrome c oxidase) is a hetero-oligomeric protein complex composed of 13 structural subunits encoded by both the mitochondrial and nuclear genomes. To study the role of nuclear-encoded...

... 344 rats. OXPHOS (oxidative phosphorylation) of intact mitochondria and cytochrome c oxidase activity in permeabilized mitochondria were determined with polarographic assays. The activities of the ETC (electron transport chain) complexes and the cytochrome content in solubilized mitochondria were...

...Maria G. Mason; Peter Nicholls; Chris E. Cooper The steady-state behaviour of isolated mammalian cytochrome c oxidase was examined by increasing the rate of reduction of cytochrome c . Under these conditions the enzyme's 605 (haem a ), 655 (haem a 3 /Cu B ) and 830 (Cu A ) nm spectral features...

..., accumulation of 3-NT paralleled the hypoxia gradient in vivo and 3-NT levels were increased in mitochondrial proteins. Liver mitochondria from mice fed the HFD for 16 weeks exhibited depressed state 3 respiration, uncoupled respiration, cytochrome c oxidase activity, and mitochondrial membrane potential...

... production of cGMP. NO also binds to the binuclear centre of COX (cytochrome c oxidase) and inhibits mitochondrial respiration in competition with oxygen and in a reversible manner. Although sGC is more sensitive to endogenous NO than COX at atmospheric oxygen tension, the more relevant question is which...

...Aimee Landar; Victor M. Darley-Usmar Haem is used as a versatile receptor for redox active molecules; most notably NO (nitric oxide) and oxygen. Three haem-containing proteins, myoglobin, haemoglobin and cytochrome c oxidase, are now known to bind NO, and in all these cases competition with oxygen...

...Claudia Piccoli; Rosella Scrima; Domenico Boffoli; Nazzareno Capitanio Recent measurements of the flux control exerted by cytochrome c oxidase on the respiratory activity in intact cells have led to a re-appraisal of its regulatory function. We have further extended this in vivo study...

...Lukas Stiburek; Katerina Vesela; Hana Hansikova; Petr Pecina; Marketa Tesarova; Leona Cerna; Josef Houstek; Jiri Zeman The biogenesis of eukaryotic COX (cytochrome c oxidase) requires several accessory proteins in addition to structural subunits and prosthetic groups. We have analysed the assembly...

... electrophoresis COX2 mutant cytochrome c oxidase haem aa 3 yeast Deficiencies in the activity of the respiratory chain enzyme cytochrome oxidase are associated with a range of diseases in humans. Mutations in the core subunits of the enzyme and in the proteins required for its assembly have been...

... as a function of age, using either NAD + - or FAD-linked substrates; however, there were no differences in state 4 respiration (ADP-depleted) rates. There was also a significant age-related decline in the activity of cytochrome c oxidase (complex IV), but not of the other mitochondrial oxidoreductases examined...

... cause of severe mitochondrial encephalomyopathies. We have investigated a rare mtDNA mutation, i.e. a 2 bp deletion of TA at positions 9205 and 9206 (9205ΔTA), which affects the STOP codon of the ATP6 gene and the cleavage site between the RNAs for ATP6 and COX3 (cytochrome c oxidase 3). The mutation...

... and immunoprecipitation analyses indicated that the protein is subunit IV of the cytochrome c oxidase complex (COIV). In vitro phosphorylation of COIV was attenuated by PKC pseudosubstrate peptides. Introduction of an PKCε-selective translocation inhibitor [Johnson, Gray, Chen and Mochly-Rosen (1996) J. Biol. Chem. 271...

...) stabilize HIF-1α at all O 2 concentrations tested. This effect does not involve the respiratory chain, since it is preserved in cells lacking functional mitochondria (ρ 0 -cells) and is not reproduced by other inhibitors of the cytochrome c oxidase. By contrast, lower concentrations of NO (<400 nM) cause...

... 2000 29 11 2000 2 1 2001 The Biochemical Society, London © 2001 2001 cytochrome c oxidase disease-associated mutations Saccharomyces cerevisiae Biochem. J. (2001) 354, 407 412 (Printed in Great Britain) 407 Site-directed mutations in the mitochondrially encoded subunits I...

...´-UTRs of nuclear-encoded mRNAs involved in the biogenesis of mitochondria have an intrinsic TEA. TEA is found in the 3´-UTR of the mRNAs encoding the α- and β-subunits of the rat H + -ATP synthase complex, as well as in subunit IV of cytochrome c oxidase. No TEA is present in the 3´-UTR of the somatic...