... mm pathlength rectangular demountable quartz cells. Scans were made between 300 and 190 nm with a band width of 1 nm and a scan speed of 100 nm/min. An average of 16 scans were measured for each experiment and a background spectrum (measured in the absence of protein) was subtracted. cytokinesis...

...Hélia Neto; Louise L. Collins; Gwyn W. Gould All cells complete cell division by the process of cytokinesis. At the end of mitosis, eukaryotic cells accurately mark the site of division between the replicated genetic material and assemble a contractile ring comprised of myosin II, actin filaments...

... unclear how pMRLC regulates myosin II and F-actin at the CR to control furrow ingression during cytokinesis. In the present study, to clarify the roles of pMRLC, we measured the turnover of myosin II and actin at the CR in dividing HeLa cells expressing fluorescent-tagged MRLCs and actin by FRAP...

... compilation © 2009 Biochemical Society 2009 cytokinesis GTP hydrolysis microtubule OTBA (3-{5-[4-oxo-2-thioxo-3-(3-trifluoromethyl-phenyl)-thiazolidin-5-ylidenemethyl]-furan-2-yl}-benzoic acid) protofilament Z-ring A conventional method of drug discovery has been to search for antibiotics from...

... are defective in endocytosis. Furthermore, towards the end of cytokinesis, constriction of the actomyosin ring and dissociation of type II myosin and septum materials are substantially delayed in the absence of functional Sip1. These results indicate that the conserved Sip1 protein comprises a novel non-SCF F...

...Jingsong Yang; Francesca Zappacosta; Roland S. Annan; Kelvin Nurse; Peter J. Tummino; Robert A. Copeland; Zhihong Lai Aurora kinases are a family of serine/threonine protein kinases that play essential roles in mitosis and cytokinesis. AurB (Aurora B kinase) has shown a clear link to cancer...

...Asuka Kato; Souichi Kurita; Aya Hayashi; Noriko Kaji; Kazumasa Ohashi; Kensaku Mizuno Cofilin regulates actin filament dynamics by stimulating actin filament disassembly and plays a critical role in cytokinesis and chemotactic migration. Aip1 (actin-interacting protein 1), also called WDR1 (WD...

...Joseph D. Dukes; Judith D. Richardson; Ruth Simmons; Paul Whitley In eukaryotic cells, the completion of cytokinesis is dependent on membrane trafficking events to deliver membrane to the site of abscission. Golgi and recycling endosomal-derived proteins are required for the terminal stages...

...Changjun Zhu; Ella Bossy-Wetzel; Wei Jiang The INCENP (inner centromere protein) is a chromosomal passenger protein that plays multiple roles in regulating mitosis and cytokinesis. The MKLP1 (mitotic kinesin-like protein) is a component of centralspindlin complex that has been implicated...

... at spermatocyte stage, but exhibits significant levels of expression in most cell types. In somatic cells, MgcRacGAP protein was found to both concentrate in the midzone/midbody and be required for cytokinesis. As a RhoGAP, MgcRacGAP has been proposed to down-regulate RhoA, which is localized to the cleavage...