... Using in silico , biochemical, enzymatic and genetic approaches, we have identified and characterized a new periplasmic thioredoxin-like protein from Neisseria meningitidis probably involved in the cytochrome c maturation process and essential for the pathogen viability cytochrome c deletion...

... of calibration, bovine carbonic anhydrase ( M r =29000), BSA ( M r =66000, yeast alcohol dehydrogenase ( M r =150000) and amylase ( M r =200000) were used as marker proteins and chromatographed under identical conditions. chloroplast import in vitro deletion mutant functional...

.... Sialic acids are important compounds of mammalian glycoconjugates, mediating several biological processes, such as cell–cell or cell–matrix interactions. In order to characterize the function of UDP- N -acetylglucosamine 2-epimerase/ N -acetylmannosamine kinase, a number of deletion mutants were...

..., the truncated mutant which removes the C-terminal lid, Δ630, and the internal deletion mutant without the mobile loop, Δ567–582, impaired the binding affinity for ThDP (thiamine diphosphate), and showed different elution profiles representing a monomeric form in gel-filtration chromatography. Our results...

... reported will appear in GenBank®, DDBJ, EMBL and GSDB Nucleotide Sequence Databases under accession numbers AY346106–AY346109. 11 8 2003 7 10 2003 24 10 2003 24 10 2003 The Biochemical Society, London ©2004 2004 adaptor protein μ chain adenosine kinase deletion mutant...