... interaction and protein–DNA interaction. In the present study, we found that the Pph3–Psy2 phosphatase complex is responsible for Rfa2 dephosphorylation both during normal G 1 -phase and under DNA replication stress in Candida albicans . Phosphorylated Rfa2 extracted from pph3 Δ or psy2 Δ G 1 cells exhibited...

...-mediated Rac-dependent changes in endothelial cell morphology. MS analysis of wild-type P-Rex1 and a PP1α-binding-deficient mutant revealed that endogenous PP1α dephosphorylates P-Rex1 on at least three residues, Ser 834 , Ser 1001 and Ser 1165 . Site-directed mutagenesis of Ser 1165 to alanine caused...

.... Although there is no direct evidence supporting dephosphorylation of the type I receptor by PP1, SARA-recruited PP1c can reduce the phosphorylation level of the type I receptor and negatively regulate Dpp signalling [ 64 ]. Thus SARA is a novel targeting subunit that recruits PP1c to the type I receptor...

.... We also show that PAPP2C effectively dephosphorylates phytochromes in vitro . Interestingly, PAPP2C indirectly mediates the dephosphorylation of PIF3 (phytochrome-interacting factor 3) in vitro . Taken together, we suggest that PAPP2C functions as a regulator of PIF3 by dephosphorylating phytochromes...

...: dephosphorylation, polyglutamine tract, protein phos- phatase-1 (PP1), SIPP1, spliceosome, splicing. INTRODUCTION Protein phosphatase-1 (PP1) is a ubiquitous protein serine/threo- nine phosphatase that functions in a variety of processes, includ- ing intermediate metabolism, protein synthesis, cell-cycle pro...

... 2002 down-regulation dephosphorylation proteasome protein phosphatase Abbreviations used: BIM-I, bisindolylmaleimide I; FCS, foetal calf serum; GFP, green fluorescent protein; HA, haemagglutinin; MEF, mouse embryonic fibroblast; PKC, protein kinase C; PP1, protein phosphatase 1; PP2A...

...-specificity MKP. This enzyme selectively interacts with, and is catalytically activated by, the ‘classical’ extracellular signal-regulated kinases (ERKs) 1 and 2 and, to a lesser extent, the stress-activated MAPK p38α. These properties define the ability of this enzyme to dephosphorylate and inactivate ERK1/2...

... Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, U.S.A. cell adhesion dephosphorylation insulin receptor Src protein tyrosine kinase PTP Biochem. J. (2001) 354, 581 590 (Printed in Great Britain) 581 Comparative study of protein tyrosine phosphatase-e isoforms : membrane...

.... 1 To whom correspondence should be addressed (e-mail [email protected] ). The Biochemical Society, London © 2001 2001 cell cycle dephosphorylation methylation PP2A tumour suppressor Biochem. J. (2001) 353, 417 439 (Printed in Great Britain) 417 REVIEW ARTICLE Protein...

... independently, mutation of either domain completely abolished the ability of NIPP1 to inhibit the dephosphorylation of myelin basic protein. The inhibitory potency of the C-terminal site of NIPP1 was decreased by phosphorylation of Tyr-335 and by the addition of RNA. Tyr-335 could be phosphorylated by tyrosine...

... as a fluorogenic substrate for protein tyrosine phosphatases (PTPs). In the peptide, the fluorescent Mca group is quenched efficiently by the DNP group. Although the fluorescence intensity of the substrate was practically unchanged upon PTP-catalysed dephosphorylation, it increased approx. 120-fold upon subsequent...

... of phosphorylase a, which prevents the dephosphorylation of glycogen synthase by PP1G. The myosin- phosphatase targeting subunits MYPT1 and MYPT2 promote the dephosphorylation of myosin by PP1 C in smooth muscle and striated muscle respectively [5,6]. MYPT1 is a substrate for phosphorylation by Rho-binding kinases...