... of a systematic gene deletion study, it was discovered that four cysteine protease-type deubiquitinases (DUBs) are essential for parasite survival in the promastigote stage, including DUB16. Here, we have purified and characterised recombinant DUB16 from Leishmania donovani , which belongs to the Ub C-terminal...

... Read & Publish agreement with Portland Press and the Biochemical Society under a transformative agreement with CAUL. deubiquitinase neurodegeneration polyglutamine repeat spinocerebellar ataxia type-3 ubiquitin proteasome system The human ATXN3 gene is located on chromosome...

.... In this study, we have characterized the biochemical and functional role of a novel ubiquitin-specific protease (USP) domain-containing protein of the human malaria parasite Plasmodium falciparum (Pf USP). We have shown that the Pf USP is an active deubiquitinase associated with parasite endoplasmic reticulum...

... hepatocellular carcinoma cells. Further investigation revealed that the deubiquitinase USP1 regulates CHK1 proteolytic degradation and that its down-regulation in our model systems is responsible, at least in part, for these effects. We demonstrate that treating WT Eµ-Myc lymphoma cells with the USP1 inhibitor...

... made by ubiquitin ligases that modulates protein abundance, localization, and/or activity. For example, some ubiquitin chains target proteins for degradation, while others function as scaffolds for the assembly of signaling complexes. Deubiquitinases (DUBs) are the proteases that counteract ubiquitin...

...Yi Wen; Li Shi; Yiluan Ding; Rong Cui; Wen-tian He; Hong-yu Hu; Naixia Zhang The deubiquitinase ubiquitin-specific protease 28 (Usp28) contains a ubiquitin-binding region (UBR) composed of one ubiquitin-associated domain (UBA) and one ubiquitin-interacting motif (UIM) at its N-terminus...

... in translocation of Crn7 to the TGN. Being a reversible PTM, ubiquitylation is regulated by deubiquitinases (DUBs) that hydrolyse isopeptide bonds between two Ub moieties or between Ub and the targeted protein [ 6 , 22 ]. The DUB TRABID (TRAF-binding domain-containing protein) was recently identified...

...Johanna Heideker; Ingrid E. Wertz The post-translational modification of proteins with ubiquitin represents a complex signalling system that co-ordinates essential cellular functions, including proteolysis, DNA repair, receptor signalling and cell communication. DUBs (deubiquitinases), the enzymes...