... the evolution of antibiotic resistance, mutations that confer drug resistance often have pleiotropic effects on the structure and function of antibiotic-target proteins, usually essential metabolic enzymes. In the present study, we show that trimethoprim (TMP)-resistant alleles of dihydrofolate reductase from...

...Melissa A. Vargo; W. Edward Martucci; Karen S. Anderson In contrast with most species, including humans, which have monofunctional forms of the folate biosynthetic enzymes TS (thymidylate synthase) and DHFR (dihydrofolate reductase), several pathogenic protozoal parasites, including Cryptosporidium...

... correspondence should be addressed (email [email protected] ). 11 9 2007 30 10 2007 2 11 2007 2 11 2007 © The Authors Journal compilation © 2008 Biochemical Society 2008 degradation dihydrofolate reductase ornithine decarboxylase (ODC) proteasome structural...

...Richard S. Swanwick; Giovanni Maglia; Lai-hock Tey; Rudolf K. Allemann The enzyme DHFR (dihydrofolate reductase) catalyses hydride transfer from NADPH to, and protonation of, dihydrofolate. The physical basis of the hydride transfer step catalysed by DHFR from Escherichia coli has been studied...

...Ningwen TAI; John C. SCHMITZ; Tian-min CHEN; Edward CHU Previous studies have shown that human DHFR (dihydrofolate reductase), in addition to its critical role in DNA biosynthesis, functions as an RNA-binding protein. The interaction between DHFR and its own mRNA results in translational repression...