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1-41 of 41
Keywords: dimerization
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Articles
Journal:
Biochemical Journal
Biochem J (2020) 477 (20): 3951–3962.
Published: 23 October 2020
... and enzymes to exhibit a wide variety of biological effects. The 3FTxs have further expanded their pharmacological space through covalent or noncovalent dimerization. Synergistic-type toxins (SynTxs) isolated from the deadly mamba venoms, although nontoxic, have been known to enhance the toxicity of other...
Includes: Supplementary data
Articles
Jinle Tang, Yingjie Wang, Huan Zhou, Yuxin Ye, Manisha Talukdar, Ziyang Fu, Zhihong Liu, Jihui Li, Dante Neculai, Jiali Gao, Hao Huang
Journal:
Biochemical Journal
Biochem J (2020) 477 (17): 3387–3399.
Published: 17 September 2020
... to other structures in PDB. Molecular dynamics simulations and dynamic light scattering results support that the binding of sunitinib in the PK domain destabilized the dimer conformation of RNase L and allosterically inhibited its ribonuclease activity. Our study suggested that dimer destabilization could...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2017) 474 (3): 427–443.
Published: 20 January 2017
... or variant amino acids. These variable residues are positioned near the zinc-binding (and dimerization) site and the stability determinant for the B-repeat fold. We have characterized four B-repeat constructs to assess the functional relevance of the two Aap B-repeat subtypes. Analytical ultracentrifugation...
Includes: Supplementary data
Articles
Victor Muleya, Claudius Marondedze, Janet I. Wheeler, Ludivine Thomas, Yee-Fong Mok, Michael D.W. Griffin, David T. Manallack, Lusisizwe Kwezi, Kathryn S. Lilley, Christoph Gehring, Helen R. Irving
Journal:
Biochemical Journal
Biochem J (2016) 473 (19): 3081–3098.
Published: 27 September 2016
... of the phosphotyrosine (Tyr888) residue in the activation loop suppressed kinase activity, while neither mutation affected guanylate cyclase activity. Size exclusion and analytical ultracentrifugation analysis of the PSKR1cd suggest that it is reversibly dimeric in solution, which was further confirmed by biflourescence...
Includes: Supplementary data
Articles
Elena Butturini, Giovanni Gotte, Daniele Dell'Orco, Giulia Chiavegato, Valerio Marino, Diana Canetti, Flora Cozzolino, Maria Monti, Piero Pucci, Sofia Mariotto
Journal:
Biochemical Journal
Biochem J (2016) 473 (19): 3205–3219.
Published: 27 September 2016
... in response to many cytokines and growth factors. Recently, the roles for unphosphorylated STAT3 (U-STAT3) have been described in response to cytokine stimulation, in cancers, and in the maintenance of heterochromatin stability. It has been reported that U-STAT3 dimerizes, shuttles between the cytoplasm...
Includes: Supplementary data
Articles
Deo R. Singh, QingQing Cao, Christopher King, Matt Salotto, Fozia Ahmed, Xiang Yang Zhou, Elena B. Pasquale, Kalina Hristova
Journal:
Biochemical Journal
Biochem J (2015) 471 (1): 101–109.
Published: 21 September 2015
... in a variety of cancers. EphA3 activation is believed to follow a ‘seeding mechanism’ model, in which ligand binding to the monomeric receptor acts as a trigger for signal-productive receptor clustering. We study EphA3 lateral interactions on the surface of live cells and we demonstrate that EphA3 forms dimers...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2015) 467 (1): 37–46.
Published: 20 March 2015
...Tilen Koklic; Rima Chattopadhyay; Rinku Majumder; Barry R. Lentz Factor Va competes factor Xa off of inactive factor Xa dimers to amplify thrombin production, both because it releases dimer inhibition and because of its cofactor activity. This suggests an unanticipated mechanism by which platelet...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2015) 465 (1): 139–147.
Published: 12 December 2014
... strongly implicated in patients with an LRRK2 mutation. Our data confirm previous assumptions that the C-terminal subdomain of COR functions as a dimerization device. We show that the dimer formation is independent of nucleotide. The affinity for GDP/GTP is in the micromolar range, the result of which...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2014) 463 (1): 83–92.
Published: 08 September 2014
...Jason C. Crack; Melanie R. Stapleton; Jeffrey Green; Andrew J. Thomson; Nick E. Le Brun The fumarate and nitrate reduction (FNR) regulator is the master switch for the transition between anaerobic and aerobic respiration in Escherichia coli . Reaction of dimeric [4Fe-4S] FNR with O 2 results...
Articles
Journal:
Biochemical Journal
Biochem J (2013) 454 (1): 49–57.
Published: 26 July 2013
... and reproduction in any medium, provided the original work is properly cited. dimerization lipid kinase activity phosphatidylinositol 5-phosphate (PtdIns5 P ) phosphatidylinositol 5-phosphate 4-kinase (PI5P4K) PI5P4Ks (PtdIns5 P 4-kinases, EC 2.7.1.149) phosphorylate PtdIns5 P to PtdIns(4,5) P...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 446 (3): 469–475.
Published: 28 August 2012
...-ular polarization, mobilization and endocytosis. The N-terminal 250 amino acids of MIM undergo homodimerization and form a structural module with the characteristic of an I-BAR [inverse BAR (Bin/amphiphysin/Rvs)] domain. To discern the role of the dimeric configuration in the function of MIM, we...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 444 (2): e3–e5.
Published: 11 May 2012
... mechanism and the substrate specificity of MALT1. These intriguing findings convincingly demonstrate the importance of MALT1 dimerization for its catalytic activity and pave the way for novel therapeutic approaches that target this crucial regulator of lymphoma survival and proliferation. dimerization...
Articles
Journal:
Biochemical Journal
Biochem J (2011) 436 (2): e1–e2.
Published: 13 May 2011
... receptors are related GTPases, and they are predicted to engage in homodimerization and/or heterodimerization. Although such dimerization has been studied extensively, its exact function in vivo remains elusive. In this issue of the Biochemical Journal , Oreb et al. present evidence that homodimerization...
Articles
Journal:
Biochemical Journal
Biochem J (2010) 429 (3): 553–563.
Published: 14 July 2010
... function in mitochondrial protein import. Based on analytical ultracentrifugation, cross-linking, size-exclusion chromatography and multi-angle light scattering, we found that the soluble cytosolic fragment of human Tom70 exists in equilibrium between monomer and dimer. A point mutation introduced...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2010) 426 (1): 99–108.
Published: 27 January 2010
... transport. In the present study, we use Blue-native PAGE to show that HPNhaA forms a dimer. Cysteine-scanning mutagenesis of residues 55–61 in a putative β-sheet region of loop1 and subsequent functional analyses revealed that the Q58C mutation resulted in an intermolecular disulfide bond. G56C, I59C...
Articles
Journal:
Biochemical Journal
Biochem J (2008) 416 (3): 357–364.
Published: 26 November 2008
... experiments carried out with His-tagged forms, gel-filtration experiments and dimerization assays provided the first unequivocal experimental results for the role of the C-terminal residues in dimerization of the enzyme. There is a hydrophobic tunnel on the surface of HTLV-1 protease close to the C-terminal...
Articles
Sabine Middelhaufe, Livia Garzia, Uta-Maria Ohndorf, Barbara Kachholz, Massimo Zollo, Clemens Steegborn
Journal:
Biochemical Journal
Biochem J (2007) 407 (2): 199–205.
Published: 25 September 2007
... 2007 4 6 2007 27 7 2007 27 7 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 dimerization metastasis non-metastatic protein 23 (NM23-H1) phosphodiesterase (PDE) protein–protein interaction prune PDE activity was measured using an SPA...
Articles
Journal:
Biochemical Journal
Biochem J (2006) 399 (3): 397–404.
Published: 13 October 2006
...-associated) domain at the C-terminus, separated by a central region containing Sti1-like repeats. Little is known about regulation of the interaction of ubiquilin with other proteins. In the present study, we show that ubiquilin is capable of forming dimers, and that dimerization requires the central region...
Articles
Richard B. Parsons, Gemma C. Price, Joanna K. Farrant, Daryl Subramaniam, Jubril Adeagbo-Sheikh, Brian M. Austen
Journal:
Biochemical Journal
Biochem J (2006) 399 (2): 205–214.
Published: 27 September 2006
...Richard B. Parsons; Gemma C. Price; Joanna K. Farrant; Daryl Subramaniam; Jubril Adeagbo-Sheikh; Brian M. Austen We have previously reported that protein lipidation in the form of palmitoylation and farnesylation is critical for the production of Aβ (amyloid β-peptide), the dimerization of β...
Articles
Journal:
Biochemical Journal
Biochem J (2006) 396 (2): 201–214.
Published: 15 May 2006
... for expression in one particular tissue or cell type. Three-dimensional structures of several S100 family members have shown that the proteins assume a dimeric structure consisting of two EF-hand motifs per monomer. Calcium binding to these S100 proteins, with the exception of S100A10, results in an approx. 40...
Articles
Journal:
Biochemical Journal
Biochem J (2006) 394 (3): 693–698.
Published: 24 February 2006
...) domain, is the dimerization domain of SH3PX1. Truncation of as little as 13 amino acid residues at the very C-terminus in the coiled-coil/BAR domain of SH3PX1 resulted in no dimerization, no ACK2-catalysed and EGF-stimulated tyrosine phosphorylation and no interaction with ACK2. The intracellular...
Articles
Hannelore PETERS, Yuri Y. KUSOV, Sonja MEYER, Andrew J. BENIE, Englbert BÄUML, Maike WOLFF, Christoph RADEMACHER, Thomas PETERS, Verena GAUSS-MÜLLER
Journal:
Biochemical Journal
Biochem J (2005) 385 (2): 363–370.
Published: 07 January 2005
...-24→Ser), C172A and C24S/C172A] to proteolysis assays with peptide substrates, and to surface plasmon resonance binding studies with peptides and viral RNA. We report that the enzyme readily forms dimers via disulphide bridges involving Cys-24. Dissociation constants ( K D ) for peptides were...
Articles
Journal:
Biochemical Journal
Biochem J (2004) 384 (3): 515–525.
Published: 07 December 2004
... show that, as the pH is lowered, the (pro)caspase dissociates, and the mutations increase the pH-dependent instability of the dimer. Overall, the results suggest that the contacts lost in the procaspase as a result of replacing Lys 242 and Glu 246 are compensated partially in the mature caspase...
Articles
David E. LANCASTER, Luke A. McNEILL, Michael A. McDONOUGH, Robin T. APLIN, Kirsty S. HEWITSON, Christopher W. PUGH, Peter J. RATCLIFFE, Christopher J. SCHOFIELD
Journal:
Biochemical Journal
Biochem J (2004) 383 (3): 429–437.
Published: 26 October 2004
... structures of FIH indicate that it is homodimeric. Site-directed mutants of FIH at residues Leu-340 and Ile-344, designed to disrupt dimerization, were generated in order to examine the importance of the dimeric state in determining FIH activity. A single point mutant, L340R (Leu-340→Arg), was shown...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2004) 378 (1): 151–159.
Published: 15 February 2004
... (from 1 to 1.7) without changing the V max . When fusion proteins were photolabelled with 8-azido-[α- 32 P]ATP, PKA phosphorylation increased the apparent k d for nucleotide binding and it caused binding to become co-operative. PKA phosphorylation also resulted in dimerization of NBD1–R–GST but not of R...
Articles
Hiroko KAWATA, Kazuya YAMADA, Zhangfei SHOU, Tetsuya MIZUTANI, Takashi YAZAWA, Miki YOSHINO, Toshio SEKIGUCHI, Takashi KAJITANI, Kaoru MIYAMOTO
Journal:
Biochemical Journal
Biochem J (2003) 373 (3): 747–757.
Published: 01 August 2003
... these come under the ZHX family. The minimal functional domains of ZHX2 were then characterized. The dimerization domain with both ZHX1 and ZHX2 is the region containing HD1, the domain that interacts with NF-YA is the HD1 to HD2 region, the repressor domain is the HD1 to a proline-rich region. Lastly, using...
Articles
Martin E. LIDELL, Malin E. V. JOHANSSON, Matthias MÖRGELIN, Noomi ASKER, James R. GUM, Jr., Young S. KIM, Gunnar C. HANSSON
Journal:
Biochemical Journal
Biochem J (2003) 372 (2): 335–345.
Published: 01 June 2003
... showed a rapid conversion of the C-terminal monomer into a dimer in both Chinese-hamster ovary-K1 and LS 174T cells. Disulphide-bond-stabilized dimers secreted into the media of both cell lines had a higher apparent molecular mass compared with the intracellular forms. The MUC2 C-terminus was purified...
Articles
Journal:
Biochemical Journal
Biochem J (2002) 368 (3): 799–808.
Published: 15 December 2002
... are the predominant MM-CKs, whereas the M3M3-CK achieves its homodimeric state at lower temperature. We postulated testable homology models to investigate the chemical properties of these dimeric interfaces. M1M1-CK was used as a reference to compare the structural differences with the M3M3-CK dimer. The calculated...
Articles
Journal:
Biochemical Journal
Biochem J (2002) 365 (2): 429–440.
Published: 15 July 2002
.... Interactions recorded between certain GPCR family members in heterologous expression systems are likely to be artefacts of extreme levels of overexpression. adrenaline dimerization energy transfer G-protein opioid Abbreviations used: BRET, bioluminescence resonance energy transfer; DADLE, [ d...
Articles
Shabih-e-Hassnain SYED, Brian TRINNAMAN, Stephen MARTIN, Sarah MAJOR, Jon HUTCHINSON, Anthony I. MAGEE
Journal:
Biochemical Journal
Biochem J (2002) 362 (2): 317–327.
Published: 22 February 2002
..., London ©2002 2002 Ca 2+ binding cell adhesion desmocollin desmoglein dimerization Abbreviations used: Dsg, desmoglein; Dsc, desmocollin; NC1, first domain of N-cadherin; Ecad1-2, the two N-terminal domains of E-cadherin; Dsg2(1-2), the two N-terminal domains of desmoglein2; Dsc2(1-2...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 360 (3): 645–649.
Published: 10 December 2001
... that both cysteine residues were involved in disulphide interactions. Substitution of either cysteine residue resulted in a reduction or loss of oligomers, although dimers of the secretory form of EGFP remained. Mutation of these residues did not adversely affect the fluorescence of EGFP. EGFP oligomers...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 357 (3): 803–810.
Published: 25 July 2001
... it appeared as monomers and dimers, but not as oligomers with high molecular masses. Therefore oligomerization is not the basis for correct localization of FT3 in the Golgi. The enzyme has been purified, with a final yield of 2% and a total purification of 2900-fold, by DEAE-Sepharose, SP-Sepharose, GDP...
Articles
Stefan PFLANZ, Thomas KERNEBECK, Bernd GIESE, Andreas HERRMANN, Michael PACHTA-NICK, Jürgen STAHL, Axel WOLLMER, Peter C. HEINRICH, Gerhard MÜLLER-NEWEN, Joachim GRÖTZINGER
Journal:
Biochemical Journal
Biochem J (2001) 356 (2): 605–612.
Published: 24 May 2001
... be addressed (e-mail [email protected] ). 2 1 2001 28 2 2001 27 3 2001 The Biochemical Society, London ©2001 2001 cytokines dimerization receptors signal transduction Abbreviations used: CBM, cytokine-binding module; FNIII, fibronectin type III; G-CSFR...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 356 (1): 87–96.
Published: 08 May 2001
.... The number of EGFR molecules was increased in cells overexpressing [K44A]dynamin, while the number of proto-oncoprotein ErbB2 molecules was unaltered. EGF-induced receptor dimerization was not influenced by overexpression of [K44A]dynamin. ErbB2–EGFR heterodimer formation was found to be ligand-independent...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 356 (1): 105–110.
Published: 08 May 2001
... reduction. The apparent K i values for nitrocatecholamine inhibition of enzyme activation by BH 4 were 18 μ M for 6-nitrodopamine and 40 μ M for 6-nitronoradrenaline. Both nitrocatecholamines antagonized the dimerization of nNOS induced by BH 4 and by l -arginine, the effect being reversed by BH 4 (more...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 353 (3): 611–620.
Published: 25 January 2001
... the sc ARE, but, although the AR fragment dimerizes on the element, the GR fragment does not. Comparing the affinities of the DNA-binding domains for mutant forms of the sc ARE revealed that dimeric GR binding is actively excluded by the left hexamer and more precisely by the presence of a G residue...
Articles
Journal:
Biochemical Journal
Biochem J (2000) 350 (1): 53–60.
Published: 09 August 2000
...Mark T. YOUNG; Roland BECKMANN; Ashley M. TOYE; Michael J. A. TANNER We have examined the mechanism by which glycophorin A (GPA) facilitates the movement of the human red-cell anion exchanger (band 3, AE1) to the cell surface. GPA itself forms stable dimers in membranes and detergent solution. Four...
Articles
Mark K. BOEHM, Adam L. CORPER, Tommy WAN, Maninder K. SOHI, Brian J. SUTTON, Jeremy D. THORNTON, Patricia A. KEEP, Kerry A. CHESTER, Richard H. J. BEGENT, Stephen J. PERKINS
Journal:
Biochemical Journal
Biochem J (2000) 346 (2): 519–528.
Published: 22 February 2000
...-mail [email protected]). The identification code for the MFE-23 co-ordinates in the Protein Data Bank is 1qok. 10 9 1999 12 11 1999 6 12 1999 The Biochemical Society, London © 2000 2000 antibody-combining site dimerization humanization immunoglobulin Biochem. J. (2000...
Articles
Nancy BURTON-WURSTER, Rina GENDELMAN, Hao CHEN, Da-Nian GU, Jonathan W. TETREAULT, George LUST, Jean E. SCHWARZBAUER, James N. MACLEOD
Journal:
Biochemical Journal
Biochem J (1999) 341 (3): 555–561.
Published: 26 July 1999
...-terminal cysteine residues essential for dimerization, but cellular and/or structural constraints appear to influence homo- and heterodimerization patterns. Dimerization patterns of the (V+C) - isoform were studied under native conditions within canine articular cartilage and experimentally in COS-7, NIH...
Articles
Journal:
Biochemical Journal
Biochem J (1999) 341 (3): 523–528.
Published: 26 July 1999
... receptor, although less efficiently than in heterodimers of wild-type receptors. The kinase-active receptor was only minimally phosphorylated. Thus the phosphorylation within the receptor dimer occurred in trans between the components. Analyses of the abilities of heterodimeric receptor complexes of one...
Articles
Journal:
Biochemical Journal
Biochem J (1999) 338 (1): 131–138.
Published: 08 February 1999
... m for ATP 9-fold. WT activity displayed a hyperbolic dependence on enzyme concentration, consistent with a requirement for dimerization or aggregation for activity. This activity was also enhanced by anti-Flag antibodies. In contrast, the dependence of DY814 activity on enzyme concentration...