... or by identifying mixed disulfide formation within cells grown in culture. We show that disulfide exchange occurs efficiently and reversibly between specific PDIs. These results have allowed us to define a hierarchy for members of the PDI family, in terms of ability to act as electron acceptors or donors during...

...Colin Shepherd; Ojore B. V. Oka; Neil J. Bulleid Disulfide formation within the endoplasmic reticulum is a complex process requiring a disulfide exchange protein such as PDI (protein disulfide-isomerase) and a mechanism to form disulfides de novo . In mammalian cells, the major pathway for de novo...

... ill-defined. The function of QSOX1 is likely to involve disulfide formation in proteins entering the secretory pathway or outside the cell. In the present study, we show that this enzyme is efficiently secreted from mammalian cells despite the presence of a transmembrane domain. We identify internal...

... disulfide formation glycosylation integral membrane protein prosequence protein assembly uroplakin The urothelial plaques that cover almost the entire mammalian urothelial apical surface provide a unique model system for studying the mechanisms and regulation of membrane assembly. Urothelial...