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Keywords: domain swapping
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Articles

Interrogation of 3D-swapped structure and functional attributes of quintessential Sortase A from Streptococcus pneumoniae

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Tora Biswas, Anurag Misra, Sreetama Das, Prity Yadav, Suryanarayanarao Ramakumar, Rajendra P. Roy
Journal: Biochemical Journal
Biochem J (2020) 477 (24): 4711–4728.
https://doi.org/10.1042/BCJ20200631
Published: 18 December 2020
... dimeric species in solution as well. crystallography domain swapping peptide substrate sortase transpeptidase SpSrtA sequence (Uniport ID: Q8DPM3) corresponding to residues, 60–247 and 82–247, respectively, was expressed in E. coli BL-21 cells as described previously [ 17...
View article titled, Interrogation of 3D-swapped structure and functional attributes of quintessential Sortase A from Streptococcus pneumoniae
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Includes: Supplementary data
Articles

Onconase dimerization through 3D domain swapping: structural investigations and increase in the apoptotic effect in cancer cells

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Andrea Fagagnini, Andrea Pica, Sabrina Fasoli, Riccardo Montioli, Massimo Donadelli, Marco Cordani, Elena Butturini, Laura Acquasaliente, Delia Picone, Giovanni Gotte
Journal: Biochemical Journal
Biochem J (2017) 474 (22): 3767–3781.
https://doi.org/10.1042/BCJ20170541
Published: 06 November 2017
... about four times after lyophilization from acetic acid solutions. Differently from RNase A (bovine pancreatic ribonuclease) and the bovine seminal ribonuclease, which produce N- and C-terminal domain-swapped conformers, ONC forms only one dimer, here named ONC-D. Cross-linking with divinylsulfone...
View article titled, Onconase dimerization through 3D domain swapping: structural investigations and increase in the apoptotic effect in cancer cells
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Includes: Supplementary data
Articles

Rational engineering of plasticity residues of sesquiterpene synthases from Artemisia annua : product specificity and catalytic efficiency

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Jian-Xu Li, Xin Fang, Qin Zhao, Ju-Xin Ruan, Chang-Qing Yang, Ling-Jian Wang, David J. Miller, Juan A. Faraldos, Rudolf K. Allemann, Xiao-Ya Chen, Peng Zhang
Journal: Biochemical Journal
Biochem J (2013) 451 (3): 417–426.
https://doi.org/10.1042/BJ20130041
Published: 12 April 2013
... synthase from A. annua , AaBOS ( A. annua α-bisabolol synthase), which has high sequence identity with AaADS ( A. annua amorpha-4,11-diene synthase), a key enzyme in artemisinin biosynthesis. Comparative analysis of the two enzymes by domain-swapping and structure-based mutagenesis led...
View article titled, Rational engineering of plasticity residues of sesquiterpene synthases from Artemisia annua : product specificity and catalytic efficiency
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Includes: Supplementary data
Articles

Phenylalanine ammonia-lyase (PAL) from tobacco ( Nicotiana tabacum ): characterization of the four tobacco PAL genes and active heterotetrameric enzymes

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Angelika I. Reichert, Xian-Zhi He, Richard A. Dixon
Journal: Biochemical Journal
Biochem J (2009) 424 (2): 233–242.
https://doi.org/10.1042/BJ20090620
Published: 11 November 2009
... database has given rise to a randomly shuffled tentative consensus sequence. The PAL2 previously described in the literature was shown, by domain swapping experiments with PAL1 , to possess a single nucleotide substitution leading to an inactive enzyme. The altered amino acid resulting from...
View article titled, Phenylalanine ammonia-lyase (PAL) from tobacco ( Nicotiana tabacum ): characterization of the four tobacco PAL genes and active heterotetrameric enzymes
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Includes: Supplementary data
Articles

Identification of dimer interactions required for the catalytic activity of the TRPM7 alpha-kinase domain

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Scott W. Crawley, Graham P. Côté
Journal: Biochemical Journal
Biochem J (2009) 420 (1): 115–122.
https://doi.org/10.1042/BJ20081405
Published: 28 April 2009
[email protected] ). 9 7 2008 3 2 2009 19 2 2009 19 2 2009 © The Authors Journal compilation © 2009 Biochemical Society 2009 alpha-kinase atypical protein kinase domain swapping ion channel transient receptor potential (TRP) channel transient receptor potential melastatin 7...
View article titled, Identification of dimer interactions required for the catalytic activity of the TRPM7 alpha-kinase domain
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Articles

Control of domain swapping in bovine odorant-binding protein

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Roberto RAMONI, Florence VINCENT, Alison E. ASHCROFT, Paolo ACCORNERO, Stefano GROLLI, Christel VALENCIA, Mariella TEGONI, Christian CAMBILLAU
Journal: Biochemical Journal
Biochem J (2002) 365 (3): 739–748.
https://doi.org/10.1042/bj20011631
Published: 01 August 2002
...Roberto RAMONI; Florence VINCENT; Alison E. ASHCROFT; Paolo ACCORNERO; Stefano GROLLI; Christel VALENCIA; Mariella TEGONI; Christian CAMBILLAU As revealed by the X-ray structure, bovine odorant-binding protein (OBPb) is a domain swapped dimer [Tegoni, Ramoni, Bignetti, Spinelli and Cambillau (1996...
View article titled, Control of domain swapping in bovine odorant-binding protein
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