... visualized GPCR signaling dynamics of five dopamine receptors and 12 serotonin receptors, and found heterogeneity between GPCRs and between cells. Even when the same Gα proteins were known to be coupled, the patterns of dynamics in GPCR downstream signaling, including the signal strength and duration, were...

... and maintenance. dopamine haemoglobin oxidative stress sympathoadrenal lineage tyrosine hydroxylase To construct a vector expressing both α-globin ( Hba-a2 ) and Eraf in mammalian cells, the EGFP sequence was removed from the pIRES2-EGFP vector (Clontech) by BstXI/NotI digestion. The BstXI end...

... dopamine and were classified as sensory neurons [ 22 ]. Moreover, in the nematode, dopamine is the only catecholamine synthesized (no adrenaline and/or noradrenaline have been detected so far) [ 23 ]. Experiments with a cat-2 -deletion mutant revealed that TH is required for the mechanosensation...

... toward nitrotyrosine. The pH-dependence and kinetic constants of SULT1A3 with nitrotyrosine or dopamine as substrate were determined. To examine whether the sulfation of nitrotyrosine occurs in the context of cellular physiology, HepG2 cells labelled with [ 35 S]sulfate were treated with SIN-1...

... that the N-terminal domain binds five Cu 2+ ions. In the present study, we have investigated copper-catalysed oxidation of a recombinant mouse prion protein, PrP 23–231 . The copper-loaded PrP 23–231 was found to be carbonylated by incubation with dopamine. Besides the formation of carbonyls, a cross-linked...

... gland extracts indicated the presence of dopa (2.18±0.82 nmol/mg of protein) and DA (dopamine, 0.06±0.02 nmol/mg of protein), but no detectable noradrenaline or adrenaline. DA was shown to derive from l -tyrosine, according to experiments performed by incubating intact ink glands with [ l - 14 C...

.... The structure–activity relationships of the human SULTs have been investigated with focus on the amino acid 146 in hSULT1A3 and its impact on dopamine/PNP ( p -nitrophenol) specificity. In the present study, we have generated canine SULT1D1 (cSULT1D1) variants with mutations at amino acid residues...

... rearrangement of dopachrome, as well as an isomerization/tautomerization of dopamine chrome and dopa methyl ester chrome. Northern hybridization revealed the transcription of yellow -f in larvae and pupae, but a high abundance of mRNA was observed in later larval and early pupal stages. In contrast, yellow-f2...

... of the endogenous cannabinoid system (ECS). Here we synthesized a series of N -acyl-dopamines (NADAs) and studied their effects on the anandamide membrane transporter, the anandamide amidohydrolase (fatty acid amide hydrolase, FAAH) and the two cannabinoid receptor subtypes, CB 1 and CB 2 . NADAs competitively...

...Lulu A. BRIX; Ronald G. DUGGLEBY; Andrea GAEDIGK; Michael E. McMANUS Human aryl sulphotransferase (HAST) 1, HAST3, HAST4 and HAST4v share greater than 90% sequence identity, but vary markedly in their ability to catalyse the sulphonation of dopamine and p -nitrophenol. In order to investigate...