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Keywords: double-mutant cycle
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Do Soo JANG, Hyung Jin CHA, Sun-Shin CHA, Bee Hak HONG, Nam-Chul HA, Ja Young LEE, Byung-Ha OH, Heung-Soo LEE, Kwan Yong CHOI
Journal:
Biochemical Journal
Biochem J (2004) 382 (3): 967–973.
Published: 07 September 2004
... ···Tyr 30 , connects two critical catalytic residues, Tyr 14 and Asp 99 , with Tyr 30 , Tyr 55 and a water molecule in the highly apolar active site of the Pseudomonas putida KSI. In order to characterize the interactions among these amino acids in the hydrogen bond network of KSI, double-mutant cycle...
