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Articles

Importance of accessibility to the extracellular juxtamembrane stalk region of membrane protein for substrate recognition by viral ubiquitin ligase K5

Mizuho Kajikawa, Mizuki Hata, Maho Ishimura, Nanae Imaizumi, Minako Kimura, Kei Miyano, Toyoyuki Ose, Daisuke Asai, Satoshi Ishido, Taisei Kanamoto

Biochem J (2022) 479 (20): 2261–2278.

https://doi.org/10.1042/BCJ20220288

Published: 28 October 2022

... of the Biochemical Society 2022 endocytosis Kaposi's sarcoma-associated herpesvirus molecular interactions transmembrane proteins ubiquitin ligases Ubiquitination is a post-translational protein modification that is highly conserved in eukaryotic cells. This modification controls various...

View article titled, Importance of accessibility to the extracellular juxtamembrane stalk region of membrane protein for substrate recognition by viral ubiquitin ligase K5

Includes: Supplementary data

Articles

Clathrin: the molecular shape shifter

Katherine M. Wood, Corinne J. Smith

Biochem J (2021) 478 (16): 3099–3123.

https://doi.org/10.1042/BCJ20200740

Published: 26 August 2021

...Katherine M. Wood; Corinne J. Smith Clathrin is best known for its contribution to clathrin-mediated endocytosis yet it also participates to a diverse range of cellular functions. Key to this is clathrin's ability to assemble into polyhedral lattices that include curved football or basket shapes...

View article titled, Clathrin: the molecular shape shifter

Articles

Crystal structure of human APPL BAR-PH heterodimer reveals a flexible dimeric BAR curve: implication in mutual regulation of endosomal targeting

Yujie Chen, Wen Zhang, Bin Chen, Ying Liu, Yuhui Dong, Aimin Xu, Quan Hao

Biochem J (2020) 477 (24): 4769–4783.

https://doi.org/10.1042/BCJ20200438

Published: 24 December 2020

... Press Limited on behalf of the Biochemical Society 2020 appl1 appl2 crystallography curvature endocytosis heterodimer To over-express APPL2 BAR-PH homodimer, the constructed modified pET28a encoding APPL2 BAR-PH domain was transformed into E. coli strain BL21(DE3). APPL2 protein...

View article titled, Crystal structure of human APPL BAR-PH heterodimer reveals a flexible dimeric BAR curve: implication in mutual regulation of endosomal targeting

Includes: Supplementary data

Articles

Molecular mechanism of Fast Endophilin-Mediated Endocytosis

Alessandra Casamento, Emmanuel Boucrot

Biochem J (2020) 477 (12): 2327–2345.

https://doi.org/10.1042/BCJ20190342

Published: 26 June 2020

...Alessandra Casamento; Emmanuel Boucrot Endocytosis mediates the cellular uptake of micronutrients and cell surface proteins. Clathrin-mediated endocytosis (CME) is the housekeeping pathway in resting cells but additional Clathrin-independent endocytic (CIE) routes, including Fast Endophilin...

View article titled, Molecular mechanism of Fast Endophilin-Mediated Endocytosis

Articles

The role of PTB domain containing adaptor proteins on PICALM-mediated APP endocytosis and localization

Lisa Merthan, Amelie Haller, Dietmar R. Thal, Bjoern von Einem, Christine A.F. von Arnim

Biochem J (2019) 476 (14): 2093–2109.

https://doi.org/10.1042/BCJ20180840

Published: 31 July 2019

... be endocytosed from the cell surface. The phosphatidylinositol binding clathrin assembly protein (PICALM) is involved in clathrin-mediated endocytosis and polymorphisms in and near the gene locus were identified as genetic risk factors for AD. PICALM overexpression enhances APP internalization and Aβ production...

View article titled, The role of PTB domain containing adaptor proteins on PICALM-mediated APP endocytosis and localization

Includes: Supplementary data

Articles

Insulin-induced exocytosis regulates the cell surface level of low-density lipoprotein-related protein-1 in Müller Glial cells

Virginia Actis Dato, Rubén A. Grosso, María C. Sánchez, Claudio M. Fader, Gustavo A. Chiabrando

Biochem J (2018) 475 (9): 1669–1685.

https://doi.org/10.1042/BCJ20170891

Published: 15 May 2018

... (LI-COR) and were quantified by densitometric analysis using Image Studio Software (LI-COR). endocytosis GTPases intracellular signaling membranes trafficking Low-density lipoprotein (LDL) receptor-related protein-1 (LRP1) is a member of the LDL receptor gene family and is expressed...

View article titled, Insulin-induced exocytosis regulates the cell surface level of low-density lipoprotein-related protein-1 in Müller Glial cells

Includes: Supplementary data

Articles

P2X7 receptor cross-talk regulates ATP-induced pannexin 1 internalization

Andrew K.J. Boyce, Leigh Anne Swayne

Biochem J (2017) 474 (13): 2133–2144.

https://doi.org/10.1042/BCJ20170257

Published: 13 June 2017

...-mediated, cholesterol-dependent P2X7R–Panx1 clustering and endocytosis. These findings have important implications for understanding the role of Panx1 in the nervous system and provide important new insights into Panx1–P2X7R cross-talk. Correspondence: Leigh Anne Swayne ( [email protected] ) 5 4...

View article titled, P2X7 receptor cross-talk regulates ATP-induced pannexin 1 internalization

Includes: Supplementary data

Articles

Deubiquitylating enzymes in receptor endocytosis and trafficking

Aidan P. McCann, Christopher J. Scott, Sandra Van Schaeybroeck, James F. Burrows

Biochem J (2016) 473 (24): 4507–4525.

https://doi.org/10.1042/BCJ20160826

Published: 09 December 2016

...Aidan P. McCann; Christopher J. Scott; Sandra Van Schaeybroeck; James F. Burrows In recent times, our knowledge of the roles ubiquitin plays in multiple cellular processes has expanded exponentially, with one example being the role of ubiquitin in receptor endocytosis and trafficking. This has...

View article titled, Deubiquitylating enzymes in receptor endocytosis and trafficking

Articles

The intricate regulation and complex functions of the Class III phosphoinositide 3-kinase Vps34

Jonathan M. Backer

Biochem J (2016) 473 (15): 2251–2271.

https://doi.org/10.1042/BCJ20160170

Published: 28 July 2016

... phosphoinositide 3-kinase endocytosis phosphoinositide 3-kinase phosphorylation/dephosphorylation Vps34 Vps34 (vacuolar protein sorting 34) is a member of the phosphoinositide 3-kinase (PI3K) family of lipid kinases, and it acts by phosphorylating phosphatidylinositol at the 3′ position on the inositol...

View article titled, The intricate regulation and complex functions of the Class III phosphoinositide 3-kinase Vps34

Articles

Septin oligomerization regulates persistent expression of ErbB2/HER2 in gastric cancer cells

Elizabeth A. Marcus, Elmira Tokhtaeva, Shahlo Turdikulova, Joseph Capri, Julian P. Whitelegge, David R. Scott, George Sachs, Fedor Berditchevski, Olga Vagin

Biochem J (2016) 473 (12): 1703–1718.

https://doi.org/10.1042/BCJ20160203

Published: 10 June 2016

..., septins protect ErbB2 from ubiquitylation, endocytosis and lysosomal degradation. The FCF-induced degradation pathway is distinct from and additive with the degradation induced by inhibiting ErbB2 chaperone Hsp90. These results identify septins as novel regulators of ErbB2 expression that contribute...

View article titled, Septin oligomerization regulates persistent expression of ErbB2/HER2 in gastric cancer cells

Includes: Supplementary data

Articles

Interdependence of ATP signalling and pannexin channels; the servant was really the master all along?

Michael F. Jackson

Biochem J (2015) 472 (3): e27–e30.

https://doi.org/10.1042/BJ20151016

Published: 27 November 2015

... by operating grant support to M.F.J. from the Canadian Institutes of Health Research (CIHR) [grant number MOP-125901]. CIE clathrin-independent endocytosis CME clathrin-mediated endocytosis EEA1 early endosome antigen 1 ER endoplasmic reticulum NMDARs N-Methyl-D-aspartic acid...

View article titled, Interdependence of ATP signalling and pannexin channels; the servant was really the master all along?

Articles

Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: a potential novel strategy to enhance zinc absorption by ZIP4 targeting

Ayako Hashimoto, Katsuma Ohkura, Masakazu Takahashi, Kumiko Kizu, Hiroshi Narita, Shuichi Enomoto, Yusaku Miyamae, Seiji Masuda, Masaya Nagao, Kazuhiro Irie, Hajime Ohigashi, Glen K. Andrews, Taiho Kambe

Biochem J (2015) 472 (2): 183–193.

https://doi.org/10.1042/BJ20150862

Published: 13 November 2015

... such soybean extracts were identified that were specifically able to decrease mZip4 endocytosis in response to zinc. These soybean extracts also effectively increased the abundance of apically localized mZip4 in transfected polarized Caco2 and Madin–Darby canine kidney cells and, moreover, two apically...

View article titled, Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: a potential novel strategy to enhance zinc absorption by ZIP4 targeting

Includes: Supplementary data

Articles

A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I

Michael D.J. Parkinson, Siân C. Piper, Nicholas A. Bright, Jennifer L. Evans, Jessica M. Boname, Katherine Bowers, Paul J. Lehner, J. Paul Luzio

Biochem J (2015) 471 (1): 79–88.

https://doi.org/10.1042/BJ20150336

Published: 21 September 2015

... ligase that promotes Lys 63 -linked polyubiquitination of MHC class I, providing the signal for clathrin-mediated endocytosis. Endocytosis is followed by sorting into the intralumenal vesicles (ILVs) of multivesicular bodies (MVBs) and eventual delivery to lysosomes. The sorting of MHC class I into MVBs...

View article titled, A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I

Articles

ATP stimulates pannexin 1 internalization to endosomal compartments

Andrew K.J. Boyce, Michelle S. Kim, Leigh E. Wicki-Stordeur, Leigh Anne Swayne

Biochem J (2015) 470 (3): 319–330.

https://doi.org/10.1042/BJ20141551

Published: 04 September 2015

... be rapidly elevated in the extracellular space. adenosine 5′-triphosphate (ATP) endocytosis endosomes pannexin 1 pannexin1 trafficking Six individual pannexin 1 (Panx1) proteins come together to form channels that facilitate the regulated passage of Ca 2+ and small molecules across...

View article titled, ATP stimulates pannexin 1 internalization to endosomal compartments

Includes: Multimedia, Supplementary data

Articles

Inositol lipid phosphatases in membrane trafficking and human disease

Peter G. Billcliff, Martin Lowe

Biochem J (2014) 461 (2): 159–175.

https://doi.org/10.1042/BJ20140361

Published: 26 June 2014

... To whom correspondence should be addressed (email [email protected] ). endocytosis human disease inositol phosphatase membrane traffic phosphoinositide secretory pathway Figure 3 Cellular distribution of inositol phosphatases Schematic depiction of the localization...

View article titled, Inositol lipid phosphatases in membrane trafficking and human disease

Articles

Trafficking modulator TENin1 inhibits endocytosis, causes endomembrane protein accumulation at the pre-vacuolar compartment and impairs gravitropic response in Arabidopsis thaliana

Rupesh Paudyal, Adam Jamaluddin, James P. Warren, Siamsa M. Doyle, Stéphanie Robert, Stuart L. Warriner, Alison Baker

Biochem J (2014) 460 (2): 177–185.

https://doi.org/10.1042/BJ20131136

Published: 13 May 2014

...) and are essential for the gravitropic response in plants. The present study characterizes an inhibitor of endomembrane protein trafficking, TE1 (trafficking and endocytosis inhibitor 1/TENin1) that reduces gravitropic root bending in Arabidopsis thaliana seedlings. Short-term TE1 treatment causes accumulation of PM...

View article titled, Trafficking modulator TENin1 inhibits endocytosis, causes endomembrane protein accumulation at the pre-vacuolar compartment and impairs gravitropic response in Arabidopsis thaliana

Includes: Supplementary data

Articles

Beyond dsRNA: Toll-like receptor 3 signalling in RNA-induced immune responses

Megumi Tatematsu, Tsukasa Seya, Misako Matsumoto

Biochem J (2014) 458 (2): 195–201.

https://doi.org/10.1042/BJ20131492

Published: 14 February 2014

... through clathrin- and raftlin-dependent endocytosis and delivered to endosomal TLR3. The dsRNA uptake machinery, in addition to TLR3, is critical for extracellular viral RNA-induced immune responses. A wide spectrum of TLR3 ligand structures beyond dsRNA and their delivery systems provide new insights...

View article titled, Beyond dsRNA: Toll-like receptor 3 signalling in RNA-induced immune responses

Articles

βTrCP interacts with the ubiquitin-dependent endocytosis motif of the GH receptor in an unconventional manner

Ana C. da Silva Almeida, Henry G. Hocking, Rolf Boelens, Ger J. Strous, Agnes G. S. H. van Rossum

Biochem J (2013) 453 (2): 291–301.

https://doi.org/10.1042/BJ20121843

Published: 28 June 2013

... by the endocytosis of the receptor via βTrCP (β-transducin repeat-containing protein). In the present study, we show that βTrCP interacts directly via its WD40 domain with the UbE (ubiquitin-dependent endocytosis) motif in GHR, promoting GHR ubiquitination in vitro . NMR experiments demonstrated that the UbE motif...

View article titled, βTrCP interacts with the ubiquitin-dependent endocytosis motif of the GH receptor in an unconventional manner

Articles

The Arf GAP AGAP2 interacts with β-arrestin2 and regulates β 2 -adrenergic receptor recycling and ERK activation

Yuanjun Wu, Yu Zhao, Xiaojie Ma, Yunjuan Zhu, Jaimin Patel, Zhongzhen Nie

Biochem J (2013) 452 (3): 411–421.

https://doi.org/10.1042/BJ20121004

Published: 31 May 2013

... ). 21 6 2012 7 3 2013 26 3 2013 26 3 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 AGAP2 β-arrestin2 Arf GTPase-activating protein endocytosis G-protein-coupled receptor recycling endosome β 2 ARs (β 2 -adrenergic receptors) are members...

View article titled, The Arf GAP AGAP2 interacts with β-arrestin2 and regulates β 2 -adrenergic receptor recycling and ERK activation

Articles

Tumour cells can employ extracellular Ins(1,2,3,4,5,6) P 6 and multiple inositol-polyphosphate phosphatase 1 (MINPP1) dephosphorylation to improve their proliferation

Sabine Windhorst, Hongying Lin, Christine Blechner, Werner Fanick, Laura Brandt, Maria A. Brehm, Georg W. Mayr

Biochem J (2013) 450 (1): 115–125.

https://doi.org/10.1042/BJ20121524

Published: 24 January 2013

... correspondence should be addressed (email [email protected] ). 3 10 2012 15 11 2012 28 11 2012 28 11 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 endocytosis Ins(1,2,3,4,5,6) P 6 (InsP 6 ) micro nutrients myo -inositol phytase phytate...

View article titled, Tumour cells can employ extracellular Ins(1,2,3,4,5,6) P 6 and multiple inositol-polyphosphate phosphatase 1 (MINPP1) dephosphorylation to improve their proliferation

Includes: Supplementary data

Articles

EHD2 shuttles to the nucleus and represses transcription

Olga Pekar, Sigi Benjamin, Hilla Weidberg, Silvia Smaldone, Francesco Ramirez, Mia Horowitz

Biochem J (2012) 444 (3): 383–394.

https://doi.org/10.1042/BJ20111268

Published: 29 May 2012

.... There are four EHD proteins in mammalian cells, EHD1–EHD4, each with diverse roles in the recycling pathway of endocytosis. EHD2 is a plasma-membrane-associated member of the EHD family that regulates internalization. Since several endocytic proteins have been shown to undergo nucleocytoplasmic shuttling...

View article titled, EHD2 shuttles to the nucleus and represses transcription

Includes: Supplementary data

Articles

Dab2 is a key regulator of endocytosis and post-endocytic trafficking of the cystic fibrosis transmembrane conductance regulator

Lianwu Fu, Andras Rab, Li Ping Tang, Steven M. Rowe, Zsuzsa Bebok, James F. Collawn

Biochem J (2012) 441 (2): 633–643.

https://doi.org/10.1042/BJ20111566

Published: 21 December 2011

...Lianwu Fu; Andras Rab; Li Ping Tang; Steven M. Rowe; Zsuzsa Bebok; James F. Collawn CFTR (cystic fibrosis transmembrane conductance regulator) is expressed in the apical membrane of epithelial cells. Cell-surface CFTR levels are regulated by endocytosis and recycling. A number of adaptor proteins...

View article titled, Dab2 is a key regulator of endocytosis and post-endocytic trafficking of the cystic fibrosis transmembrane conductance regulator

Articles

EHD2 mediates trafficking from the plasma membrane by modulating Rac1 activity

Sigi Benjamin, Hilla Weidberg, Debora Rapaport, Olga Pekar, Marina Nudelman, Daniel Segal, Koret Hirschberg, Shulamit Katzav, Marcelo Ehrlich, Mia Horowitz

Biochem J (2011) 439 (3): 433–445.

https://doi.org/10.1042/BJ20111010

Published: 13 October 2011

...Sigi Benjamin; Hilla Weidberg; Debora Rapaport; Olga Pekar; Marina Nudelman; Daniel Segal; Koret Hirschberg; Shulamit Katzav; Marcelo Ehrlich; Mia Horowitz EHDs [EH (Eps15 homology)-domain-containing proteins] participate in different stages of endocytosis. EHD2 is a plasma-membrane-associated EHD...

View article titled, EHD2 mediates trafficking from the plasma membrane by modulating Rac1 activity

Includes: Supplementary data

Articles

Ubiquitin-mediated internalization of connexin43 is independent of the canonical endocytic tyrosine-sorting signal

Steve Catarino, José S. Ramalho, Carla Marques, Paulo Pereira, Henrique Girão

Biochem J (2011) 437 (2): 255–267.

https://doi.org/10.1042/BJ20102059

Published: 28 June 2011

... receptor substrate 15. 1 To whom correspondence should be addressed (email [email protected] ). 16 12 2010 21 4 2011 9 5 2011 9 5 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 connexin43 (Cx43) endocytosis epidermal growth factor...

View article titled, Ubiquitin-mediated internalization of connexin43 is independent of the canonical endocytic tyrosine-sorting signal

Includes: Multimedia, Supplementary data

Articles

Functional analysis of Dictyostelium IBARa reveals a conserved role of the I-BAR domain in endocytosis

Douwe M. Veltman, Giulio Auciello, Heather J. Spence, Laura M. Machesky, Joshua Z. Rappoport, Robert H. Insall

Biochem J (2011) 436 (1): 45–52.

https://doi.org/10.1042/BJ20101684

Published: 27 April 2011

... with clathrin plaques, although its homologues IRSp53 and IRTKS (insulin receptor tyrosine kinase substrate) associate with other punctate structures. The results from the present study suggest that I-BAR-containing proteins help generate the mem-brane curvature required for endocytosis and implies...

View article titled, Functional analysis of Dictyostelium IBARa reveals a conserved role of the I-BAR domain in endocytosis

Includes: Supplementary data

Articles

Visualization of retrovirus uptake and delivery into acidic endosomes

Kosuke Miyauchi, Mariana Marin, Gregory B. Melikyan

Biochem J (2011) 434 (3): 559–569.

https://doi.org/10.1042/BJ20101588

Published: 24 February 2011

...Kosuke Miyauchi; Mariana Marin; Gregory B. Melikyan Diverse enveloped viruses enter cells by endocytosis and fusion with intracellular compartments. Recent evidence suggests that HIV also infects permissive cell lines by fusing with endosomes in a pH-independent manner. This finding highlights...

View article titled, Visualization of retrovirus uptake and delivery into acidic endosomes

Includes: Supplementary data

Articles

The deubiquitinating enzyme DUB2A enhances CSF3 signalling by attenuating lysosomal routing of the CSF3 receptor

Annemarie Meenhuis, Carola Verwijmeren, Onno Roovers, Ivo P. Touw

Biochem J (2011) 434 (2): 343–351.

https://doi.org/10.1042/BJ20101628

Published: 11 February 2011

... 2011 endocytosis deubiquitinating enzyme (DUB) granulocyte colony-stimulating factor 3 (CSF3) receptor signal transducer and activator of transcription (STAT) 5 suppressor of cytokine signalling (SOCS) 3 HEK-293T (human embryonic kidney cells expressing the large T-antigen of simian virus...

View article titled, The deubiquitinating enzyme DUB2A enhances CSF3 signalling by attenuating lysosomal routing of the CSF3 receptor

Articles

The myotubularin family of lipid phosphatases in disease and in spermatogenesis

Dolores D. Mruk, C. Yan Cheng

Biochem J (2011) 433 (2): 253–262.

https://doi.org/10.1042/BJ20101267

Published: 22 December 2010

... in spermatogenic defects. Other studies have shown that MTMR2 functions in endocytosis and membrane trafficking. In the testis, MTMR2 interacts and co-localizes with c-Src/phospho-Src-(Tyr 416 ), a non-receptor protein tyrosine kinase that regulates the phosphorylation state of proteins at the apical ES...

View article titled, The myotubularin family of lipid phosphatases in disease and in spermatogenesis

Includes: Supplementary data

Articles

Specific amino acids in the BAR domain allow homodimerization and prevent heterodimerization of sorting nexin 33

Bastian Dislich, Manuel E. Than, Stefan F. Lichtenthaler

Biochem J (2011) 433 (1): 75–83.

https://doi.org/10.1042/BJ20100709

Published: 15 December 2010

...Bastian Dislich; Manuel E. Than; Stefan F. Lichtenthaler SNX33 (sorting nexin 33) is a homologue of the endocytic prote-in SNX9 and has been implicated in actin polymerization and the endocytosis of the amyloid precursor protein. SNX33 belongs to the large family of BAR (Bin/amphiphysin/Rvs) domain...

View article titled, Specific amino acids in the BAR domain allow homodimerization and prevent heterodimerization of sorting nexin 33

Includes: Supplementary data

Articles

Endocytosis of hyaluronidase-1 by the liver

Marie-Christine Gasingirwa, Jacqueline Thirion, Jeannine Mertens-Strijthagen, Simone Wattiaux-De Coninck, Bruno Flamion, Robert Wattiaux, Michel Jadot

Biochem J (2010) 430 (2): 305–313.

https://doi.org/10.1042/BJ20100711

Published: 13 August 2010

...Marie-Christine Gasingirwa; Jacqueline Thirion; Jeannine Mertens-Strijthagen; Simone Wattiaux-De Coninck; Bruno Flamion; Robert Wattiaux; Michel Jadot It has been suggested that intracellular Hyal-1 (hyaluronidase-1), which is considered a lysosomal enzyme, originates via endocytosis of the serum...

View article titled, Endocytosis of hyaluronidase-1 by the liver

Articles

Transferrin-iron routing to the cytosol and mitochondria as studied by live and real-time fluorescence

Maya Shvartsman, Eitan Fibach, Z. Ioav Cabantchik

Biochem J (2010) 429 (1): 185–193.

https://doi.org/10.1042/BJ20100213

Published: 14 June 2010

...Maya Shvartsman; Eitan Fibach; Z. Ioav Cabantchik In the present study we analysed the mechanism of intracellular routing of iron acquired by erythroid cells via receptor-mediated endocytosis of Tf-Fe [Tf (transferrin)-iron]. Using real-time fluorimetry and flow cytometry, in conjunction...

View article titled, Transferrin-iron routing to the cytosol and mitochondria as studied by live and real-time fluorescence

Articles

Adaptor protein 2 regulates receptor-mediated endocytosis and cyst formation in Giardia lamblia

Maria R. Rivero, Cecilia V. Vranych, Mariano Bisbal, Belkys A. Maletto, Andrea S. Ropolo, Maria C. Touz

Biochem J (2010) 428 (1): 33–45.

https://doi.org/10.1042/BJ20100096

Published: 28 April 2010

..., suggesting its involvement in endocytosis. Uptake experiments using standard molecules for the study of endocytosis revealed that gAP2 specifically participated in the endocytosis of LDL (low-density lipoprotein). Targeted down-regulation of the gene encoding gμ2 in growing and encysting trophozoites...

View article titled, Adaptor protein 2 regulates receptor-mediated endocytosis and cyst formation in Giardia lamblia

Includes: Supplementary data

Articles

Analysis of the dual function of the ESCRT-III protein Snf7 in endocytic trafficking and in gene expression

Peter Weiss, Stefanie Huppert, Ralf Kölling

Biochem J (2009) 424 (1): 89–97.

https://doi.org/10.1042/BJ20090957

Published: 23 October 2009

... © The Authors Journal compilation © 2009 Biochemical Society 2009 endocytosis endosomal sorting complex required for transport (ESCRT) multivesicular body Rim101 pathway Snf7/chromatin modifying protein 4 (CHMP4) vacuolar protein sorting During endocytosis, cell surface proteins...

View article titled, Analysis of the dual function of the ESCRT-III protein Snf7 in endocytic trafficking and in gene expression

Articles

Dynasore inhibits removal of wild-type and ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) from the plasma membrane

Andrew Young, Martina Gentzsch, Cynthia Y. Abban, Ying Jia, Patricio I. Meneses, Robert J. Bridges, Neil A. Bradbury

Biochem J (2009) 421 (3): 377–385.

https://doi.org/10.1042/BJ20090389

Published: 15 July 2009

...Andrew Young; Martina Gentzsch; Cynthia Y. Abban; Ying Jia; Patricio I. Meneses; Robert J. Bridges; Neil A. Bradbury Dynasore, a small molecule inhibitor of dynamin, was used to probe the role of dynamin in the endocytosis of wild-type and mutant CFTR (cystic fibrosis transmembrane conductance...

View article titled, Dynasore inhibits removal of wild-type and ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) from the plasma membrane

Articles

Low concentration thresholds of plasma membranes for rapid energy-independent translocation of a cell-penetrating peptide

Catherine L. Watkins, Dirk Schmaljohann, Shiroh Futaki, Arwyn T. Jones

Biochem J (2009) 420 (2): 179–191.

https://doi.org/10.1042/BJ20090042

Published: 13 May 2009

... min prior to incubation with 2 μM R L 8–Alexa488 at 4 °C, and analysed as described above. © The Authors Journal compilation © 2009 Biochemical Society 2009 cell-penetrating peptide (CPP) cholesterol endocytosis fluorescence microscopy methyl-β-cyclodextrin (MβCD) octa-arginine...

View article titled, Low concentration thresholds of plasma membranes for rapid energy-independent translocation of a cell-penetrating peptide

Includes: Multimedia, Supplementary data

Articles

Identification of a conserved F-box protein 6 interactor essential for endocytosis and cytokinesis in fission yeast

Isabelle Jourdain, Nathalie Spielewoy, James Thompson, Susheela Dhut, John R. Yates, III, Takashi Toda

Biochem J (2009) 420 (2): 169–180.

https://doi.org/10.1042/BJ20081659

Published: 13 May 2009

... are defective in endocytosis. Furthermore, towards the end of cytokinesis, constriction of the actomyosin ring and dissociation of type II myosin and septum materials are substantially delayed in the absence of functional Sip1. These results indicate that the conserved Sip1 protein comprises a novel non-SCF F...

View article titled, Identification of a conserved F-box protein 6 interactor essential for endocytosis and cytokinesis in fission yeast

Includes: Multimedia, Supplementary data

Articles

Channel-interacting PDZ protein, ‘CIPP’, interacts with proteins involved in cytoskeletal dynamics

Emanuele Alpi, Elena Landi, Manuela Barilari, Michela Serresi, Piero Salvadori, Angela Bachi, Luciana Dente

Biochem J (2009) 419 (2): 289–300.

https://doi.org/10.1042/BJ20081387

Published: 27 March 2009

... acid. The tryptic digests where desalted and concentrated when needed, before loading sample on to the target. © The Authors Journal compilation © 2009 Biochemical Society 2009 actin/tubulin remodelling channel-interacting PDZ protein (CIPP) Cypin endocytosis insulin receptor tyrosine...

View article titled, Channel-interacting PDZ protein, ‘CIPP’, interacts with proteins involved in cytoskeletal dynamics

Includes: Supplementary data

Articles

Dab2 regulates clathrin assembly and cell spreading

David Chetrit, Naomi Ziv, Marcelo Ehrlich

Biochem J (2009) 418 (3): 701–715.

https://doi.org/10.1042/BJ20081288

Published: 25 February 2009

... phosphoprotein and a monomeric adaptor for clathrin-mediated endocytosis. In the present study, we employed GFP (green fluorescent protein) fusion constructs of different isoforms and mutants of rat Dab2 and characterized their effect on the size, distribution and dynamics of clathrin assemblies. Enhanced levels...

View article titled, Dab2 regulates clathrin assembly and cell spreading

Includes: Multimedia, Supplementary data

Articles

Janus kinases promote cell-surface expression and provoke autonomous signalling from routing-defective G-CSF receptors

Annemarie Meenhuis, Mahban Irandoust, Albert Wölfler, Onno Roovers, Marijke Valkhof, Ivo P. Touw

Biochem J (2009) 417 (3): 737–746.

https://doi.org/10.1042/BJ20081153

Published: 16 January 2009

...). In addition to their role in phosphorylation of receptor tyrosine residues and downstream signalling substrates, JAKs have recently been implicated in controlling expression of cytokine receptors, predominantly by masking critical motifs involved in endocytosis and lysosomal targeting. In the present study...

View article titled, Janus kinases promote cell-surface expression and provoke autonomous signalling from routing-defective G-CSF receptors

Articles

Glycosylphosphatidylinositol-specific phospholipase C regulates transferrin endocytosis in the African trypanosome

Sandesh Subramanya, C. Frank Hardin, Dietmar Steverding, Kojo Mensa-Wilmot

Biochem J (2009) 417 (3): 685–694.

https://doi.org/10.1042/BJ20080167

Published: 16 January 2009

... the virulence of a pleomorphic strain of the parasite, for reasons that are not clear. In the present paper, we report that GPI-PLC stimulates endocytosis of transferrin by 300–500%. Surprisingly, GPI-PLC is not detected at endosomes, suggesting that the enzyme does not interact directly with the endosomal...

View article titled, Glycosylphosphatidylinositol-specific phospholipase C regulates transferrin endocytosis in the African trypanosome

Articles

Sorting out PtdIns(4,5) P 2 and clathrin-coated vesicles in plants

Charles Brearley

Biochem J (2008) 415 (3): e1–e3.

https://doi.org/10.1042/BJ20081830

Published: 15 October 2008

... in plants. A study in this issue of the Biochemical Journal by König et al. reports for the first time in this kingdom the association of PtdIns(4,5) P 2 with an endomembrane fraction enriched for clathrin. This work is discussed in the context of current evidence for constitutive and evoked endocytosis...

View article titled, Sorting out PtdIns(4,5) P 2 and clathrin-coated vesicles in plants

Articles

CD95 ligation and intracellular membrane flow

Roland Reinehr, Dieter Häussinger

Biochem J (2008) 413 (3): e11–e12.

https://doi.org/10.1042/BJ20081094

Published: 15 July 2008

.... report another aspect on endocytosis in response to CD95 ligation in type II cells, namely a caspase-independent endocytosis with vesicle translocation to the mitochondrial compartment, suggestive of an interplay between both organelles in the sense of an ‘organelle scrambling’. Thus early effects...

View article titled, CD95 ligation and intracellular membrane flow

Articles

Endosomal compartment contributes to the propagation of CD95/Fas-mediated signals in type II cells

Paola Matarrese, Valeria Manganelli, Tina Garofalo, Antonella Tinari, Lucrezia Gambardella, Kenneth Ndebele, Roya Khosravi-Far, Maurizio Sorice, Mauro Degli Esposti, Walter Malorni

Biochem J (2008) 413 (3): 467–478.

https://doi.org/10.1042/BJ20071704

Published: 15 July 2008

... encompasses a series of subcellular changes that are mandatory for, or even bolster, the apoptotic cascade. In the present study, we analysed the role of endocytosis in the propagation of cell death signalling after CD95/Fas engagement in type II cells (CEM cells). We show that this receptor–ligand...

View article titled, Endosomal compartment contributes to the propagation of CD95/Fas-mediated signals in type II cells

Articles

Hepatocyte growth factor activator inhibitor-1 has a complex subcellular itinerary

Sine Godiksen, Joanna Selzer-Plon, Esben D. K. Pedersen, Kathrine Abell, Hanne B. Rasmussen, Roman Szabo, Thomas H. Bugge, Lotte K. Vogel

Biochem J (2008) 413 (2): 251–259.

https://doi.org/10.1042/BJ20071496

Published: 26 June 2008

... plasma membrane. 1 To whom correspondence should be addressed (e-mail [email protected] ). endocytosis hepatocyte growth factor activator inhibitor-1 (HAI-1) lysosomal degradation proteolytic release transcytosis 31 10 2007 7 4 2008 10 4 2008 10 4 2008 ©...

View article titled, Hepatocyte growth factor activator inhibitor-1 has a complex subcellular itinerary

Articles

Focusing on clathrin-mediated endocytosis

Joshua Z. Rappoport

Biochem J (2008) 412 (3): 415–423.

https://doi.org/10.1042/BJ20080474

Published: 28 May 2008

...Joshua Z. Rappoport Investigations into the mechanisms which regulate entry of integral membrane proteins, and associated ligands, into the cell through vesicular carriers (endocytosis) have greatly benefited from the application of live-cell imaging. Several excellent recent reviews have detailed...

View article titled, Focusing on clathrin-mediated endocytosis

Articles

Lactoferrin receptor mediates apo- but not holo-lactoferrin internalization via clathrin-mediated endocytosis in trophoblasts

Veronica Lopez, Shannon L. Kelleher, Bo Lönnerdal

Biochem J (2008) 411 (2): 271–278.

https://doi.org/10.1042/BJ20070393

Published: 27 March 2008

... to a LfR of ∼105 kDa. Apo-Lf treatment significantly increased LfR abundance at the plasma membrane and internalization probably occurs via clathrin-mediated endocytosis through early and recycling endosomes, as LfR was co-localized with EEA1 (early endosome antigen 1) and TfR (transferrin receptor) using...

View article titled, Lactoferrin receptor mediates apo- but not holo-lactoferrin internalization via clathrin-mediated endocytosis in trophoblasts

Articles

Enhanced cell-surface stability of rescued ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) by pharmacological chaperones

Karoly Varga, Rebecca F. Goldstein, Asta Jurkuvenaite, Lan Chen, Sadis Matalon, Eric J. Sorscher, Zsuzsa Bebok, James F. Collawn

Biochem J (2008) 410 (3): 555–564.

https://doi.org/10.1042/BJ20071420

Published: 27 February 2008

... show that rΔF508 CFTR endocytosis is 6-fold more rapid (∼30% per 2.5 min) than WT (wild-type, ∼5% per 2.5 min) CFTR at 37 °C in polarized airway epithelial cells (CFBE41o − ). We also investigated rΔF508 CFTR endocytosis under two further conditions: in culture at the permissive temperature (27 °C...

View article titled, Enhanced cell-surface stability of rescued ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) by pharmacological chaperones

Articles

Regulation of clathrin-dependent endocytosis by diacylglycerol kinase δ: importance of kinase activity and binding to AP2α

Takumi Kawasaki, Takeshi Kobayashi, Takehiko Ueyama, Yasuhito Shirai, Naoaki Saito

Biochem J (2008) 409 (2): 471–479.

https://doi.org/10.1042/BJ20070755

Published: 21 December 2007

... the molecular mechanism of DGKδ-mediated regulation of clathrin-dependent endocytosis that controls the internalization, recycling and degradation of receptors. Involvement of DGKδ in the regulation of clathrin-dependent endocytosis was previously proposed following genome-wide RNAi (RNA interference) screening...

View article titled, Regulation of clathrin-dependent endocytosis by diacylglycerol kinase δ: importance of kinase activity and binding to AP2α

Articles

Hetero-oligomerization of the P2Y 11 receptor with the P2Y 1 receptor controls the internalization and ligand selectivity of the P2Y 11 receptor

Denise Ecke, Theodor Hanck, Mohan E. Tulapurkar, Rainer Schäfer, Matthias Kassack, Rolf Stricker, Georg Reiser

Biochem J (2008) 409 (1): 107–116.

https://doi.org/10.1042/BJ20070671

Published: 11 December 2007

... internalization of the P2Y 11 receptor. This is remarkable because the P2Y 11 receptor by itself is not able to undergo endocytosis. Co-internalization of these receptors was also seen in 1321N1 astrocytoma cells co-expressing both P2Y 11 and P2Y 1 receptors, upon stimulation with ATP or the P2Y 1 receptor...

View article titled, Hetero-oligomerization of the P2Y 11 receptor with the P2Y 1 receptor controls the internalization and ligand selectivity of the P2Y 11 receptor

Includes: Supplementary data

Articles

Epithelial sodium channel (ENaC) is multi-ubiquitinated at the cell surface

Dominik Wiemuth, Ying Ke, Meino Rohlfs, Fiona J. Mc Donald

Biochem J (2007) 405 (1): 147–155.

https://doi.org/10.1042/BJ20060747

Published: 13 June 2007

... for their internalization by clathrin-mediated endocytosis and subsequent trafficking, our results support a model whereby ubiquitin and clathrin adaptor binding sites act in concert to remove ENaC from the cell surface. 1 Present address: Department of Physiology II, Institute of Physiology, University...

View article titled, Epithelial sodium channel (ENaC) is multi-ubiquitinated at the cell surface

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