1-50 of 99
Keywords: endoplasmic reticulum
Close
Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Articles
Biochem J (2020) 477 (17): 3183–3197.
Published: 04 September 2020
... biotinylation, Ca 2+ imaging as well as protein knockdown and overexpression of a dominant-negative TRPC6 mutant (TRPC6dn) to show that TRPC6 and STIM2 are required for the maintenance of cytosolic and endoplasmic reticulum Ca 2+ content under resting conditions in ER+ breast cancer MCF7 cells. These cells...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (21): 3241–3260.
Published: 11 November 2019
...Sindhu Wisesa; Yasunori Yamamoto; Toshiaki Sakisaka The tubular network of the endoplasmic reticulum (ER) is formed by connecting ER tubules through three-way junctions. Two classes of the conserved ER membrane proteins, atlastins and lunapark, have been shown to reside at the three-way junctions...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (13): 1929–1942.
Published: 09 July 2019
... believed to be derived by a budding and scission process from the surface of the endoplasmic reticulum, and this occurs concomitantly with the accumulation of neutral lipids, most often triacylglycerols and steryl esters. Overall, the mechanisms underlying LD biogenesis are difficult to generalize, in part...
Articles
Biochem J (2018) 475 (6): 1037–1057.
Published: 20 March 2018
...Alex B. Addinsall; Craig R. Wright; Sof Andrikopoulos; Chris van der Poel; Nicole Stupka Chronic metabolic stress leads to cellular dysfunction, characterized by excessive reactive oxygen species, endoplasmic reticulum (ER) stress and inflammation, which has been implicated in the pathogenesis of...
Articles
Biochem J (2018) 475 (5): 873–886.
Published: 06 March 2018
... mutants, but impair the secretion of others. However, ectopic glycans that enhanced secretion could not functionally replace a native N -glycan in the same domain. Secretion-deficient mutants, but not mutants with elevated secretion levels, show increased association with the endoplasmic reticulum...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (4): 827–838.
Published: 28 February 2018
... function. The enzyme methionine sulfoxide reductase (Msr) reverses this modification. Here, we characterise the mammalian enzyme Msr B3. There are two splice variants of this enzyme that differ only in their N-terminal signal sequence, which directs the protein to either the endoplasmic reticulum (ER) or...
Articles
Biochem J (2017) 474 (18): 3179–3188.
Published: 08 September 2017
.... Taken together, our results demonstrate that the variable domain can influence the folding of the C H 1 domain, so that it can fold in the absence of its cognate LC. antibodies antibody folding antibody production antibody secretion endoplasmic reticulum The ability to humanise...
Articles
Biochem J (2016) 473 (7): 851–858.
Published: 29 March 2016
... topology, with the large loop between transmembrane one and two facing the lumen of the endoplasmic reticulum (ER). We used a redox sensitive green fluorescent protein (GFP) fused to the N- or C-terminus to show that these regions face the cytosol, and introduction of glycosylation sites along with mixed...
Articles
Biochem J (2016) 473 (4): 397–410.
Published: 09 February 2016
...Qin Wang; Yongqiang Wang; Gregory P. Downey; Sergey Plotnikov; Christopher A. McCulloch Ca 2+ release is tightly sequestered in eukaryotic cells to enable fine spatio-temporal control of signalling but how Ca 2+ release from the endoplasmic reticulum (ER) is linked to cell adhesions is not defined...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (3): 233–244.
Published: 25 January 2016
... that impair biogenesis of the transporter cause type I cystinuria. It has been shown that upon assembly, b 0,+ AT prevents degradation and promotes folding of rBAT; then, rBAT traffics b 0,+ AT from the endoplasmic reticulum (ER) to the plasma membrane. The role of the N-glycans of rBAT and of its C...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (1): 1–5.
Published: 09 December 2015
...G. Cristina Brailoiu; Elena Deliu; Linda M. Console-Bram; Jonathan Soboloff; Mary E. Abood; Ellen M. Unterwald; Eugen Brailoiu Sigma-1 receptor (Sig-1R) is an intracellular chaperone protein with many ligands, located at the endoplasmic reticulum (ER). Binding of cocaine to Sig-1R has previously...
Articles
Biochem J (2015) 469 (2): 279–288.
Published: 06 July 2015
... exchange reactions. We show in the present study that disulfide exchange can occur directly between individual PDI proteins. Implication is that only certain members need to be oxidized or reduced to maintain function. disulfide formation electron transfer endoplasmic reticulum protein disulfide...
Articles
Biochem J (2015) 466 (3): 455–465.
Published: 06 March 2015
...+ probes to specifically measure Ca 2+ in the cis/cis- to -medial -Go (cGo) or the trans -Go (tGo). Ca 2+ homoeostasis in these compartments and in the endoplasmic reticulum (ER) has been studied and compared. Moreover, the relative size of each subcompartment was estimated from aequorin consumption. We...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2014) 464 (3): 401–411.
Published: 05 December 2014
...Silvia Hüttner; Christiane Veit; Ulrike Vavra; Jennifer Schoberer; Martina Dicker; Daniel Maresch; Friedrich Altmann; Richard Strasser N -glycosylation of proteins plays an important role in the determination of the fate of newly synthesized glycoproteins in the endoplasmic reticulum (ER). Specific...
Articles
Biochem J (2014) 464 (2): 241–249.
Published: 14 November 2014
...Ji Won Park; James R. Reed; Lauren M. Brignac-Huber; Wayne L. Backes Cytochrome P450 (P450) function is dependent on the ability of these enzymes to successfully interact with their redox partners, NADPH-cytochrome P450 reductase (CPR) and cytochrome b 5 , in the endoplasmic reticulum (ER). Because...
Includes: Supplementary data
Articles
Biochem J (2014) 464 (1): e5–e6.
Published: 23 October 2014
...Saverio Marchi; Paolo Pinton In this issue of the Biochemical Journal , Wu et al. describe the characterization of new low-affinity Ca 2+ indicators for monitoring Ca 2+ levels in both the ER (endoplasmic reticulum) and mitochondria. In contrast with other ER Ca 2+ sensors, these indicators emit in...
Articles
Biochem J (2014) 463 (3): 373–381.
Published: 10 October 2014
... by STIM1 is poorly understood. STIM1 is a single transmembrane protein that communicates the filling state of the endoplasmic reticulum to store-operated channels. STIM1 has been reported to regulate the activity of all of the TRPC family members, except TRPC7. TRPC6 has been predominantly associated...
Articles
Biochem J (2014) 462 (1): 133–142.
Published: 24 July 2014
... channel trafficking generally result in an increase in retention of the channel complex in the ER (endoplasmic reticulum) [ 11 ]. Impaired channel assembly tends to be associated with nonsense mutations because they truncate the protein before helices C and D which are critical for tetramerization [ 12...
Articles
Biochem J (2014) 461 (1): 107–113.
Published: 13 June 2014
...Colin Shepherd; Ojore B. V. Oka; Neil J. Bulleid Disulfide formation within the endoplasmic reticulum is a complex process requiring a disulfide exchange protein such as PDI (protein disulfide-isomerase) and a mechanism to form disulfides de novo . In mammalian cells, the major pathway for de novo...
Articles
Biochem J (2014) 459 (1): 47–58.
Published: 14 March 2014
... non-polar hydrophobic chemical composition of TAGs and the aqueous environment of the blood, but it is also significant as it creates a process which can be regulated, fitting to the variable dietary needs of organisms. VLDLs are synthesized in the ER (endoplasmic reticulum) and transported to the...
Articles
Biochem J (2014) 457 (1): 99–105.
Published: 10 December 2013
...Thea Bismo Strøm; Kristian Tveten; Trond P. Leren PCSK9 (proprotein convertase subtilisin/kexin type 9) binds to the LDLR (low-density lipoprotein receptor) at the cell surface and disrupts recycling of the LDLR. However, PCSK9 also interacts with the LDLR in the ER (endoplasmic reticulum). In the...
Includes: Supplementary data
Articles
Biochem J (2013) 456 (2): 297–309.
Published: 08 November 2013
...Helen R. Watson; Lydia Wunderley; Tereza Andreou; Jim Warwicker; Stephen High The majority of the polytopic proteins that are synthesized at the ER (endoplasmic reticulum) are integrated co-translationally via the Sec61 translocon, which provides lateral access for their hydrophobic TMs...
Includes: Supplementary data
Articles
Biochem J (2013) 451 (3): 345–352.
Published: 12 April 2013
... Pex3 as a docking factor for Pex19 and its designation as a class II PMP [ 67 ]. biogenesis endoplasmic reticulum peroxin peroxisome peroxisome membrane protein quality control Peroxisomes are multifunctional dynamic organelles present in all eukaryotes with the exception of the...
Articles
Biochem J (2013) 450 (2): 321–332.
Published: 15 February 2013
...Nader T. Amin; A. Katrine Wallis; Stephen A. Wells; Michelle L. Rowe; Richard A. Williamson; Mark J. Howard; Robert B. Freedman ERp27 (endoplasmic reticulum protein 27.7 kDa) is a homologue of PDI (protein disulfide-isomerase) localized to the endoplasmic reticulum. ERp27 is predicted to consist of...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (1): 127–139.
Published: 26 April 2012
...Elena S. Dremina; Victor S. Sharov; Christian Schöneich We have demonstrated previously that Bcl-2 and Bcl-2Δ21, a C-terminally truncated Bcl-2 sequence, inactivate SERCA (sarcoplasmic/endoplasmic reticulum Ca 2+ -ATPase) 1 in isolated SR (sarcoplasmic reticulum), accompanied by a translocation...
Articles
Biochem J (2012) 442 (3): 639–648.
Published: 24 February 2012
... tools for the dissection of complex biological processes. ES I (eeyarestatin I) is a novel modulator of ER (endoplasmic reticulum) function. In the present study, we show that in addition to acutely inhibiting ERAD (ER-associated degradation), ES I causes production of mislocalized polypeptides that are...
Includes: Supplementary data
Articles
Biochem J (2012) 441 (2): 707–717.
Published: 21 December 2011
... network). In the present study, brefeldin A treatment of tendon explant cultures showed that N-proteinase activity is present in the resulting fused ER (endoplasmic reticulum)–Golgi compartment, but that C-proteinase activity is restricted to the TGN in embryonic chick tendon fibroblasts. In late...
Articles
Biochem J (2012) 441 (1): 105–112.
Published: 14 December 2011
...Gaëlle Tahay; Elsa Wiame; Donatienne Tyteca; Pierre J. Courtoy; Emile Van Schaftingen Aspartate N-acetyltransferase (NAT8L, N-acetyltransferase 8-like), the enzyme that synthesizes N -acetylaspartate, is membrane-bound and is at least partially associated with the ER (endoplasmic reticulum). The...
Includes: Supplementary data
Articles
Biochem J (2011) 437 (3): 469–475.
Published: 13 July 2011
...Sonia Gallego-Sandín; María Teresa Alonso; Javier García-Sancho CALHM1 (calcium homoeostasis modulator 1), a membrane protein with similarity to NMDA ( N -methyl- D -aspartate) receptor channels that localizes in the plasma membrane and the ER (endoplasmic reticulum) of neurons, has been shown to...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (2): 509–518.
Published: 29 March 2011
... demonstrated delayed glycan maturation kinetics upon substitution of the lysine residues. Moreover, VSVG-wt EPO-R, but not VSVG-5KR EPO-R, displayed endoplasmic reticulum-associated ubiquitination. Despite similar cell-surface EPO-binding levels of both receptors and the lack of EPO-induced ubiquitination by...
Articles
Biochem J (2011) 435 (1): 227–235.
Published: 15 March 2011
... Ca 2+ stores using targeted aequorins for selective measurements in different subcellular compartments. Both, HEK-293T [HEK (human embryonic kidney)-293 cells expressing the large T-antigen of SV40 (simian virus 40)] and HeLa cells accumulated Ca 2+ into the ER (endoplasmic reticulum) to near...
Includes: Supplementary data
Articles
Biochem J (2011) 434 (2): 181–188.
Published: 11 February 2011
...Min Ni; Yi Zhang; Amy S. Lee GRP78 (glucose-regulated protein of 78 kDa) is traditionally regarded as a major ER (endoplasmic reticulum) chaperone facilitating protein folding and assembly, protein quality control, Ca 2+ binding and regulating ER stress signalling. It is a potent anti-apoptotic...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2010) 430 (3): 497–510.
Published: 27 August 2010
...Yiguo Zhang; John D. Hayes Nrf1 [NF-E2 (nuclear factor-erythroid 2)-related factor 1] is a CNC (cap'n'collar) bZIP (basic-region leucine zipper) transcription factor that is tethered to ER (endoplasmic reticulum) and nuclear envelope membranes through its N-terminal signal peptide (residues 1–30...
Includes: Supplementary data
Articles
Biochem J (2010) 427 (3): 513–521.
Published: 14 April 2010
...Richard S. Marshall; Lorenzo Frigerio; Lynne M. Roberts The ER (endoplasmic reticulum) has long been considered the plant cell compartment within which protein disulfide bond formation occurs. Members of the ER-located PDI (protein disulfide isomerase) family are responsible for oxidizing, reducing...
Articles
Biochem J (2010) 426 (2): 135–145.
Published: 09 February 2010
...Josh Duffy; Bhargavi Patham; Kojo Mensa-Wilmot N-terminal signal peptides direct secretory proteins into the ER (endoplasmic reticulum) of eukaryotes or the periplasmic space of prokaryotes. A hydrophobic core (h-region) is important for signal sequence function; however, the mechanism of h-region...
Includes: Supplementary data
Articles
Biochem J (2010) 425 (1): 61–74.
Published: 14 December 2009
...Evangelia Pantazaka; Colin W. Taylor Targeting of IP 3 R (inositol 1,4,5-trisphosphate receptors) to membranes of the ER (endoplasmic reticulum) and their retention within ER or trafficking to other membranes underlies their ability to generate spatially organized Ca 2+ signals. N-terminal...
Includes: Supplementary data
Articles
Biochem J (2010) 425 (1): 195–208.
Published: 14 December 2009
...Doris Roth; Emily Lynes; Jan Riemer; Henning G. Hansen; Nils Althaus; Thomas Simmen; Lars Ellgaard The thiol-disulfide oxidoreductases of the PDI (protein disulfide isomerase) family assist in disulfide-bond formation in the ER (endoplasmic reticulum). In the present study, we have shown that the...
Includes: Supplementary data
Articles
Biochem J (2009) 423 (2): 145–155.
Published: 25 September 2009
...Imogen A. Sparkes; Lorenzo Frigerio; Nicholas Tolley; Chris Hawes The ER (endoplasmic reticulum) in higher plants forms a pleomorphic web of membrane tubules and small cisternae that pervade the cytoplasm, but in particular form a polygonal network at the cortex of the cell which may be anchored to...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2009) 418 (3): 553–566.
Published: 25 February 2009
...Joel Castro; Edoardo C. Aromataris; Grigori Y. Rychkov; Greg J. Barritt The question of whether the activation of SOCs (store-operated Ca 2+ channels) requires the whole or part of the ER (endoplasmic reticulum) has not been fully resolved. The role of a putative sub-compartment of the ER in SOC...
Includes: Supplementary data
Articles
Biochem J (2008) 410 (3): 463–472.
Published: 27 February 2008
... characterize the localization and dynamics of ACBP in living cells. Results showed that ACBP targeted to the ER (endoplasmic reticulum) and Golgi in a ligand-binding-dependent manner. A variant Y28F/K32A-FACI-50, which is unable to bind acyl-CoA, did no longer show association with the ER and became segregated...
Includes: Supplementary data
Articles
Biochem J (2008) 410 (2): 409–416.
Published: 12 February 2008
... present study, we addressed the role of the EPO-R intracellular domain in exit from the ER (endoplasmic reticulum) and surface expression. A fusion protein between the thermo-reversible folding mutant of VSVG (vesicular-stomatitis-virus glycoprotein) (VSVGtsO45) and the EPO-R cytosolic domain [VSVG-WT...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2008) 410 (1): 93–100.
Published: 29 January 2008
... classified into Q-SNARE and R-SNARE based on the structural property of their motifs. Assembly of four SNARE motifs (Qa, b, c and R) is supposed to trigger membrane fusion. We have previously shown that ER (endoplasmic reticulum)-localized syntaxin 18 (Qa) forms a complex with BNIP1 (Qb), p31/Use1 (Qc...
Articles
Biochem J (2007) 408 (2): 161–172.
Published: 14 November 2007
... homology box 1; residues 11–30), which tethers Nrf1 to the ER (endoplasmic reticulum). Electrophoresis resolved Nrf1 into two major bands of approx. 95 and 120 kDa. The 120-kDa Nrf1 form represents a glycosylated protein that was present exclusively in the ER and was converted into a substantially smaller...
Includes: Supplementary data
Articles
Biochem J (2007) 404 (3): 467–476.
Published: 29 May 2007
...Kevin Larade; Zhi-gang Jiang; Andre Dejam; Hao Zhu; H. Franklin Bunn The novel reductase NCB5OR (NADPH cytochrome b 5 oxidoreductase) resides in the ER (endoplasmic reticulum) and may protect cells against ER stress. Levels of BiP (immunoglobulin heavy-chain-binding protein), CHOP (CCAAT/enhancer...
Articles
Biochem J (2007) 401 (3): 701–709.
Published: 12 January 2007
... oil tree [ Vernicia ( Aleurites ) fordii ] tail-anchored Cb5 (cytochrome b 5 ) target specifically to ER (endoplasmic reticulum) membranes both in vivo and in vitro [Hwang, Pelitire, Henderson, Andrews, Dyer and Mullen (2004) Plant Cell 16 , 3002–3019]. In the present study, we examine the targeting...
Includes: Supplementary data
Articles
Biochem J (2007) 401 (2): 607–612.
Published: 21 December 2006
... single membrane spanning domain, and its C-terminus located in the ER (endoplasmic reticulum) lumen. However, in the present study we show that torsinA is not in fact an integral membrane protein. Instead we find that the mature protein associates peripherally with the ER membrane, most likely through an...
Articles
Biochem J (2006) 399 (3): 373–385.
Published: 13 October 2006
... controlled by the NTD (N-terminal domain) through its ability to direct Nrf1 to the endoplasmic reticulum. Ectopic expression of wild-type Nrf1 and mutants lacking either the NTD or portions of its Neh2-like subdomain into wild-type and mutant mouse embryonic fibroblasts indicated that Keap1 controls neither...
Includes: Supplementary data
Articles
Biochem J (2006) 396 (2): 265–275.
Published: 15 May 2006
... associated with the endoplasmic reticulum and with Rab5-positive vesicles. However, this mutant is complex-glycosylated like the wt protein. D157G and G323V mutants have a defective iron export capacity as judged by their inability to deplete the intracellular ferritin content, whereas Q182H and delV162 have...
Includes: Supplementary data
Articles
Biochem J (2006) 396 (1): 173–182.
Published: 26 April 2006
...Karin Osibow; Sasa Frank; Roland Malli; Rudolf Zechner; Wolfgang F. Graier Considering the physiological Ca 2+ dynamics within the ER (endoplasmic reticulum), it remains unclear how efficient protein folding is maintained in living cells. Thus, utilizing the strictly folding-dependent activity and...
Articles
Biochem J (2006) 395 (2): 249–258.
Published: 28 March 2006
... expression of a novel human mRNA variant encoding a yet uncharacterized SERCA [SR (sarcoplasmic reticulum)/ER (endoplasmic reticulum) Ca 2+ -ATPase] protein, SERCA2c [Gélébart, Martin, Enouf and Papp (2003) Biochem. Biophys. Res. Commun. 303 , 676–684]. In the present study, we have analysed the expression...
Includes: Supplementary data