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Keywords: enzyme activity
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Articles
Biochem J (2021) 478 (11): 2081–2099.
Published: 08 June 2021
... 6 5 2021 6 5 2021 Correspondence: Vinay Kumar Nandicoori ( vinaykn@nii.ac.in ) cell wall synthesis enzyme activity GlmU protein–protein interactions specificity tuberculosis References 1 WHO . ( 2019 ) Global Tuberculosis Report Geneva , World Health...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (6): 1179–1201.
Published: 27 March 2020
... at −80°C until measurement of enzyme activity. Table 2. Real-time PCR conditions and primer sequences for analysis of gene expression Gene Primers Amplicon (bp) FUT1 5′-ACTATTGGCACCTTTGGCT-3′ 5′-ATTGATGCCCACCCACTCAG-3′ 144 FUT2 5′-AATAGTGAAGCTCCAAACCC-3′ 5...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (5): 953–970.
Published: 06 March 2020
... 15 2 2020 18 2 2020 18 2 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 catalysis enzyme activity fluorescence resonance energy transfer plasminogen streptokinase Human plasminogen (HPG), a single chain...
Articles
Biochem J (2019) 476 (20): 2939–2952.
Published: 18 October 2019
... enzymes exhibited high affinity for oxaloacetate and ATP, reinforcing their role as decarboxylases. We employed a high-throughput screening for putative allosteric regulators using differential scanning fluorometry and confirmed their effect on enzyme activity by performing enzyme kinetics. Ath PEPCK1 and...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (18): 2579–2594.
Published: 20 September 2019
... Society 2019 biosynthesis enzyme activity evolutionary biology genomics metabolism polyamines Cellular growth and proliferation in all eukaryotes, most archaea and many bacteria are likely contingent on the presence of the polyamine spermidine [ 1 ]. The pyruvoyl-dependent enzyme S...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (4): 665–682.
Published: 19 February 2019
... antibody–drug conjugation enzyme activity Gram-positive bacteria sorting transpeptidation Sortases are bacterial enzymes, which anchor proteins to the cell wall of Gram-positive bacteria or participate in pilus subunit polymerization. Both enzymatic activities have an important physiological...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (21): 3377–3391.
Published: 09 November 2018
... analysis indicated this region to be intrinsically disordered. The present study provides information on the structural and functional regulation, protein-substrate(s) interactions and dynamics, and the role of non-catalytic domains in regulating the activity of FgNRS. enzyme activity intrinsically...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (20): 3275–3291.
Published: 31 October 2018
... and oxidation, and that these modifications regulate enzyme activity by preventing the formation of the active tetrameric form. The biotin-switch assay carried out on M1 and M2 isoforms showed that M2PYK is sensitive to nitrosation and that Cys326 is highly susceptible to redox modification...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (13): 2219–2233.
Published: 21 June 2017
... 10 5 2017 11 5 2017 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 enzyme activity enzymology molecular evolution protein structure ribonuclease RNA Vertebrate animals possess a distinct, conserved family of secreted...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (21): 3837–3854.
Published: 27 October 2016
...] indicate the concentrations of total SiR and free Fd in the reaction mixture, respectively. electron transfer complex enzyme activity ferredoxin isothermal titration calorimetry nuclear magnetic resonance sulfite reductase The physicochemical and structural aspects of the surfaces and...
Includes: Supplementary data
Articles
Biochem J (2013) 451 (2): 217–226.
Published: 28 March 2013
... structure enzyme activity protein engineering substrate specificity transition state analogue Cephalosporins are currently the most widely prescribed class of antibiotics on the market and are used to treat both Gram-positive and Gram-negative bacteria (reviewed in [ 1 ]). They act by inhibiting...
Includes: Supplementary data
Articles
Biochem J (2009) 421 (1): 107–118.
Published: 12 June 2009
.... falciparum PRMT1) has authentic type I PRMT activity to form monomethylarginines and asymmetric dimethylarginines. Compared with mammalian PRMT1s, PfPRMT1 possesses a distinctive N-terminal sequence that is ∼50 amino acids longer and is essential for enzyme activity. Recombinant PfPRMT1 methylated histones...
Includes: Supplementary data
Articles
Biochem J (2009) 417 (1): 55–64.
Published: 12 December 2008
... used throughout. enzyme activity glutathione transferase (GST) protein folding protein structure GSTs (glutathione transferases; EC 2.5.1.18) are a large family of enzymes involved in cellular detoxification; they catalyse the reduction of electrophilic substrates into non-harmful...
Articles
Biochem J (2007) 402 (2): 331–337.
Published: 12 February 2007
...Michelle E. Peterson; Roy M. Daniel; Michael J. Danson; Robert Eisenthal Traditionally, the dependence of enzyme activity on temperature has been described by a model consisting of two processes: the catalytic reaction defined by Δ G Dagger cat , and irreversible inactivation defined by Δ G Dagger...
Articles
Biochem J (2005) 386 (2): 263–270.
Published: 22 February 2005
...) was from Sigma (St. Louis, MO, U.S.A.). All other chemicals were of reagent grade and purchased from Nacalai Tesque (Kyoto, Japan). All spectrophotometric measurements were done with a Shimadzu UV-2200 spectrophotometer (Kyoto, Japan). enzyme activity matrix metalloproteinase matrilysin...
Articles
Biochem J (2004) 382 (2): 725–731.
Published: 24 August 2004
... TALΔS171 had no enzymic activity. TALΔS171 was effectively translated in vitro using rabbit reticulocyte lysates, indicating that the absence of TAL-H protein in TALΔS171 fibroblasts and lymphoblasts may be attributed primarily to rapid degradation. Treatment with cell-permeable proteasome inhibitors led...
Articles
Biochem J (2000) 352 (2): 251–255.
Published: 24 November 2000
... multiple-site mutant. 1 To whom correspondence should be addressed (e-mail joze.pungercar@ijs.si ). 7 8 2000 18 8 2000 The Biochemical Society, London © 2000 2000 enzyme activity receptor-binding affinity site-directed mutagenesis Vipera ammodytes ammodytes Biochem. J...
Articles
Biochem J (2000) 349 (3): 813–819.
Published: 25 July 2000
... unique ligand binding properties, HSA also possesses an esterase-like activity, and studies with p -nitrophenyl acetate as a substrate showed that, although Arg-410 is important, the enzymic activity of HSA is much more dependent on the presence of Tyr-411. A minor activity could be registered when...
Articles
Biochem J (2000) 345 (2): 351–356.
Published: 10 January 2000
...). 3 3 1999 10 9 1999 5 11 1999 The Biochemical Society, London © 2000 2000 enzyme activity fluorescent kinetic assay metallopeptidase Biochem. J. (2000) 345, 351 356 (Printed in Great Britain) 351 Development and characterization of novel potent and stable inhibitors of...
Articles
Biochem J (1999) 341 (1): 139–145.
Published: 24 June 1999
..., which is 17 times less toxic. Two mutants of AtxA have been produced in bacteria and characterized. The specific enzymic activity of the K128 → E mutant on mixed phosphatidylcholine/Triton X-100 micelles is similar to that of the wild type. The K108 → N/K111 → N mutant, however, possesses 160% of the...
Articles
Biochem J (1999) 337 (3): 503–506.
Published: 25 January 1999
... © 1999 1999 enzyme activity β-fructofuranosidase fructosyltransferase LsdA site-directed mutagenesis Biochem. J. (1999) 337, 503 506 (Printed in Great Britain) 503 Substitution of Asp-309 by Asn in the Arg-Asp-Pro (RDP) motif of Acetobacter diazotrophicus levansucrase affects sucrose...